Difference between revisions of "Os08g0509600"
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===Mutation=== | ===Mutation=== | ||
| − | Jiao et al. demonstrate that a point mutation in OsSPL14 perturbs OsmiR156-directed regulation of OsSPL14, generating an‘ideal’ rice plant with a reduced tiller number, increased lodging resistance and enhanced grain yield<ref name="ref3" />. | + | *The white boxes in figure 2 represent the 5′and 3′untranslated regions, the black boxes in figure 2 represent the coding sequences and lines between boxes represent introns. The red asterisk in figure 2 indicates the OsmiR156 target site<ref name="ref3" />. |
| + | |||
| + | *Previous studies suggested that 11 OsSPL genes were putative targets of OsmiR156, a member of an miRNA family consisting of 19–25-base-pair noncoding single-stranded regulatory RNAs. Bioinformatic analysis indicated that OsSPL14 has the OsmiR156 complementary site in the coding region<ref name="ref3" />. | ||
| + | |||
| + | *Fig. 3b, indicating that perturbing the OsmiR156 cleavage of OsSPL14 even without changing the resulting amino acid sequence leads to alteration of the plant architecture in SNJ. Fig. 3c and 3d, suggesting that the point mutation may also perturb the OsmiR156-directed translation repression of OsSPL14. Therefore, the point mutation in the OsmiR156-complementary site of OsSPL14 perturbs the OsmiR156-directed transcriptional cleavage and translation repression in rice<ref name="ref3" />. | ||
| + | |||
| + | *Jiao et al. demonstrate that a point mutation in OsSPL14 perturbs OsmiR156-directed regulation of OsSPL14, generating an‘ideal’ rice plant with a reduced tiller number, increased lodging resistance and enhanced grain yield<ref name="ref3" />. | ||
===Expression=== | ===Expression=== | ||
Revision as of 08:38, 6 June 2014
OsSPL14, a member of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes[1], and plays an important role in controlling rice plant architecture as well as yield [2].
Contents
Annotated Information
Function
- The rice gene OsSPL14 plays an important role in controlling rice plant architecture as well as yield. OsSPL14 encodes a SQUAMOSA promoter-binding-like (SPL) protein, as a gene that can affect plant architecture[2].Figure 1 shows that OsSPL14 regulates plant morphology and influences rice grain yield. (a) Classic rice plants are highly tillered with relatively small panicles. (b) In contrast, the new plant type (NPT) architecture is characterized by fewer tillers and more productive panicles. Normally, OsmiR156 induces cleavage of OsSPL14 transcripts, leading to a repression of OsSPL14 activity. However, mutation of an OsmiR156 binding site within OsSPL14 increases the levels of OsSPL14 and results in rice plants with fewer tillers but more productive panicles and consequently higher grain yields[2].
Figure 1. OsSPL14 regulates plant morphology and influences rice grain yield(from reference)[2].
- Higher expression of OsSPL14 in the reproductive stage promotes panicle branching and higher grain yield in rice. OsSPL14 controls shoot branching in the vegetative stage and is affected by microRNA excision[3].
Mutation
- The white boxes in figure 2 represent the 5′and 3′untranslated regions, the black boxes in figure 2 represent the coding sequences and lines between boxes represent introns. The red asterisk in figure 2 indicates the OsmiR156 target site[4].
- Previous studies suggested that 11 OsSPL genes were putative targets of OsmiR156, a member of an miRNA family consisting of 19–25-base-pair noncoding single-stranded regulatory RNAs. Bioinformatic analysis indicated that OsSPL14 has the OsmiR156 complementary site in the coding region[4].
- Fig. 3b, indicating that perturbing the OsmiR156 cleavage of OsSPL14 even without changing the resulting amino acid sequence leads to alteration of the plant architecture in SNJ. Fig. 3c and 3d, suggesting that the point mutation may also perturb the OsmiR156-directed translation repression of OsSPL14. Therefore, the point mutation in the OsmiR156-complementary site of OsSPL14 perturbs the OsmiR156-directed transcriptional cleavage and translation repression in rice[4].
- Jiao et al. demonstrate that a point mutation in OsSPL14 perturbs OsmiR156-directed regulation of OsSPL14, generating an‘ideal’ rice plant with a reduced tiller number, increased lodging resistance and enhanced grain yield[4].
Expression
Luo et al. confirm that OsSPL14 is expressed in the leaf primordia but is excluded from the meristematic cells[1].
Figure 2 shows expression pattern of OsSPL14. (A, B, D, E, G and H) RNA in situ hybridization with an OsSPL14 probe. (C, F and I) RNA in situ hybridization using an OSH1 probe. (A) The shoot apical meristem at transition. (B) and (C) A panicle at the secondary branch initiation stage. (D) Transverse view of the panicle at the secondary branch initiation stage. (E) and (F) Immature spikelets. (G) Axillary meristem. (H) and (I) Axillary meristem at a later stage. SAM, shoot apical meristem; br, bract primordia; fm, flower meristem; le, lemma; pa, palea; sl, sterile lemma; rg, rudimentary glumes; AM, axillary meristem; lp, leaf primordia. Asterisks indicate the primary and secondary branch meristems[1].
Evolution
SPL protein are plant-specific transcription factors containing a highly conserved DNA-binding domain called an SBP-box. The founder members of the SPL family, SBP1 and SBP2, were identified in Antirrhinum majus as proteins that bind to a sequence motif present in the promoter of SQUAMOSA (SQUA), the MADS-box gene which specifies flower meristem identity. SPL genes are widely conserved in both dicots and monocots. In maize, a few SPL genes have been identified, including liguleless1 (LG1), teosinte glume architecture 1 (tga1) and tasselsheath4 (TSH4)[1].
The rice OsSPL14 gene is the closest homolog of the Arabidopsis SPL9 and SPL15 genes and one of the 11 OsmiR156-targeted SPL genes of rice[1].
Localization
OsSPL14 is localized to the nucleus (Figure 3), consistent with a role as a transcription factor[4].
Knowledge Extension
The SPL genes make up a plant-specific multigene family of transcription factors that can play roles in regulating plant morphology and the decision to flower[2].
Some of the SPL genes are controlled by miR156 that controls phase transitions in plants. In Arabidopsis, 10 out of 16 SPL genes broadly control developmental transition and are targets of miR156. The expression level of miR156 is high in the early stage of shoot development, and decreases with time. Accordingly, the mRNA level of miR156-targeted SPL genes gradually increases as development proceeds, in agreement with its role in the temporal control of shoot development. In Arabidopsis, the targets of miR156 are divided into two classes, one containing SPL3, SPL4 and SPL5, encoding small proteins, and the other containing genes encoding longer proteins. Generally, genes in both groups are involved in the control of temporal development; in particular, in phase transition and flowering. Among members of the second group, SPL9 and SPL15 are expressed during the vegetative phase and function in the control of leaf initiation[1].
Labs working on this gene
- State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
- Graduate School of Agriculture and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8657, Japan
- Department of Bioscience, Fukui Prefectural University, 4-1-1 Matsuoka Kenjyojima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan
- Bioscience and Biotechnology Center, Nagoya University, Nagoya, Aichi 464-8601, Japan
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Luo L, Li W, Miura K, Ashikari M, Kyozuka J. Control of tiller growth of rice by OsSPL14 and Strigolactones, which work in two independent pathways[J]. Plant & cell physiology, 2012,53(10):1793-1801.
- ↑ 2.0 2.1 2.2 2.3 2.4 Springer N. Shaping a better rice plant[J]. Nat Genet, 2010,42(6):475-476.
- ↑ Miura K, Ikeda M, Matsubara A, Song X-J, Ito M, Asano K, et al. OsSPL14 promotes panicle branching and higher grain productivity in rice[J]. Nat Genet, 2010,42(6):545-549.
- ↑ 4.0 4.1 4.2 4.3 4.4 4.5 Jiao Y, Wang Y, Xue D, Wang J, Yan M, Liu G, et al. Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice[J]. Nat Genet, 2010,42(6):541-544.
Structured Information
| Gene Name |
Os08g0509600 |
|---|---|
| Description |
Similar to Squamosa-promoter binding-like protein 8 |
| Version |
NM_001068739.1 GI:115477215 GeneID:4345998 |
| Length |
4156 bp |
| Definition |
Oryza sativa Japonica Group Os08g0509600, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 8:25362546..25366701 |
| Sequence Coding Region |
25362793..25363463,25363566..25363699,25366130..25366578 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008401:25362546..25366701 source=RiceChromosome08 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008401:25362546..25366701 source=RiceChromosome08 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggagatggccagtggaggaggcgccgccgccgccgccggcggcggagtaggcggcagcggcggcggtggtggtggaggggacgagcaccgccagctgcacggtctcaagttcggcaagaagatctacttcgaggacgccgccgcggcagcaggcggcggcggcactggcagtggcagtggcagcgcgagcgccgcgccgccgtcctcgtcttccaaggcggcgggtggtggacgcggcggagggggcaagaacaaggggaagggcgtggccgcggcggcgccaccgccgccgccgccgccgccgcggtgccaggtggaggggtgcggcgcggatctgagcgggatcaagaactactactgccgccacaaggtgtgcttcatgcattccaaggctccccgcgtcgtcgtcgccggcctcgagcagcgcttctgccagcagtgcagcaggttccacctgctgcctgaatttgaccaaggaaaacgcagctgccgcagacgccttgcaggtcataatgagcgccggaggaggccgcaaacccctttggcatcacgctacggtcgactagctgcatctgttggtgagcatcgcaggttcagaagctttacgttggatttctcctacccaagggttccaagcagcgtaaggaatgcatggccagcaattcaaccaggcgatcggatctccggtggtatccagtggcacaggaacgtagctcctcatggtcactctagtgcagtggcgggatatggtgccaacacatacagcggccaaggtagctcttcttcagggccaccggtgttcgctggcccaaatctccctccaggtggatgtctcgcaggggtcggtgccgccaccgactcgagctgtgctctctctcttctgtcaacccagccatgggatactactacccacagtgccgctgccagccacaaccaggctgcagccatgtccactaccaccagctttgatggcaatcctgtggcaccctccgccatggcgggtagctacatggcaccaagcccctggacaggttctcggggccatgagggtggtggtcggagcgtggcgcaccagctaccacatgaagtctcacttgatgaggtgcaccctggtcctagccatcatgcccacttctccggtgagcttgagcttgctctgcaggggaacggtccagccccagcaccacgcatcgatcctgggtccggcagcaccttcgaccaaaccagcaacacgatggattggtctctgtag</cdnaseq> |
| Protein Sequence |
<aaseq>MEMASGGGAAAAAGGGVGGSGGGGGGGDEHRQLHGLKFGKKIYF EDAAAAAGGGGTGSGSGSASAAPPSSSSKAAGGGRGGGGKNKGKGVAAAAPPPPPPPP RCQVEGCGADLSGIKNYYCRHKVCFMHSKAPRVVVAGLEQRFCQQCSRFHLLPEFDQG KRSCRRRLAGHNERRRRPQTPLASRYGRLAASVGEHRRFRSFTLDFSYPRVPSSVRNA WPAIQPGDRISGGIQWHRNVAPHGHSSAVAGYGANTYSGQGSSSSGPPVFAGPNLPPG GCLAGVGAATDSSCALSLLSTQPWDTTTHSAAASHNQAAAMSTTTSFDGNPVAPSAMA GSYMAPSPWTGSRGHEGGGRSVAHQLPHEVSLDEVHPGPSHHAHFSGELELALQGNGP APAPRIDPGSGSTFDQTSNTMDWSL</aaseq> |
| Gene Sequence |
<dnaseqindica>3239..3909#3003..3136#124..572#ttccgtctctttcctctctcttctctctccccctctcctggaggagagagaggagaagaggagggggggccgcgccaagagccacgcgcgctacagtctccttcccacccgcgaccgcgagcaatggagatggccagtggaggaggcgccgccgccgccgccggcggcggagtaggcggcagcggcggcggtggtggtggaggggacgagcaccgccagctgcacggtctcaagttcggcaagaagatctacttcgaggacgccgccgcggcagcaggcggcggcggcactggcagtggcagtggcagcgcgagcgccgcgccgccgtcctcgtcttccaaggcggcgggtggtggacgcggcggagggggcaagaacaaggggaagggcgtggccgcggcggcgccaccgccgccgccgccgccgccgcggtgccaggtggaggggtgcggcgcggatctgagcgggatcaagaactactactgccgccacaaggtgtgcttcatgcattccaaggctccccgcgtcgtcgtcgccggcctcgagcagcgcttctgccagcagtgcagcaggtcactctctcactcacctcgccattgctgatgtcaccactgcttttgctttgctttgcttgctctccctcctctttcacctatctctcttgtttatttgcttcttgttcttgtttagtgctagtacatgtgttgttattgttgtgccgttttgtcttttgggttattgtgttgttgttactactcgttttactataggtttttaaggtttatgagcacggccaccacattagatgcactgtcaagtggtgtgtgtgggacctttcctgctaaaacaagctgatttcaactctctgaaacttcctgcatttcatctatttttatctttgattgtgttgggagtactacactagtagtgttaatattttgactggtgcttatgagatttttaagttggtaggttgatgaggaaaatactcctttatatggttgagtgatgtgacttgcctgtctgcctgcctgcctgccgctttgcataagattcctctgtgttagtaagagccactgtttatttgtactggtgcttactctacttagttaattagccattagctataaaattccgttgatgttgcaagcttagcaatggccacggtaagaatgggagagagaagttggctaaagctgttgctttgtagtttgtactatatatgtgtctttgtgttgcaagatatgcaactcctactatgctgtgacttgagctcaaggttttcagttatctatagatccttactactactgagcatactaccacttctgtatggtagcatatggtagcatagtccaagttccaacgcctcgccagttgttcataatctatactaccacttctgtgcatttgttacttttatttaatagtttgtctcattagctgacaagcatatgcctgttttgatatctgcccctcttgtaatagtctatggatagcttggactgtttgatgctttaattttttactagcaacacttagggcccctttgaaatggaggattagcaaaggaattttggaggattcattttcctaaggattttttcctatagagccctttgattcatagaaagaggataggaaaacttccgtaggattgcattcctatgatcaattccataggaaaataagcaagaggttagacctcttgtgaaactttcctttgttgagtgtatcttgtggtataatcaaagggctcttctctccatttcatgtgttttcaattcctgtaggattggaaaaacatacaacttcaattcctacgtttttcctattcctatgtttttcctatcctgcgtttcaaaggggcccttaaggatgaagggaagtaagagaaacatactagagaatatgtagtagtatttctacattccatatttgtagcactagcccacaaatatctttgccttgtacttacttcataccagttccccccttttcagagcaaaccaacaatttctgttgccttatatatctagtgtcttcgtactaatatatctgttccaaaatgtacctgtccaaattcatagctagaaatagctttatttaggacggaagtaataactgttgttagagacttggttcagacttttggttatgttgaggctactatcatttcctttacgggccaaattactacaaatgagaattcataaaaatgtcaagattttatgattgttgtagctttatttaggacggaggtagtaattgttgttagagacttggttcagacttttggttacgttgaagctactatcatttcctttatggtcaaattactaacaatgagtattcataaaaatgtcaagattttataattgagctgtgccagtgctaagtgtgtcactatctgatgccataatgcatcattataaaagccagatggaccattagcttttatgtgtaggacacctgccgtccaattagatggataaccatctagtgtttgtgtactgttattttaagcccgacatctcacaactccatgaatgattacagtcttcctttcacatggtgtccttttgttgtgttaggaatagcattttttatttatgggtgtaattatgaaaggcactaggagagttgctgctttatcttgatgggatttgtagtaataccatctttaggatgacaagaaatcttgttctgagttagcatgggctgccttttgacctgagctacggtttgctatgtttggcttgcatcatgcagatctattaggataataagcatataaaagttgcttgcattgtgcattgcttgttttaccttgattcatgtaggagtaatttgctcgccatgcctcgttttgctttctgagtcaacagccaaatttagatgatgtaccttctgttgcttcaaaaactcagtcactgcacagcagcagtggataggattcagaatcaatctatccatgattctctgttcacataatatgacaggttccacctgctgcctgaatttgaccaaggaaaacgcagctgccgcagacgccttgcaggtcataatgagcgccggaggaggccgcaaacccctttggcatcacgctacggtcgactagctgcatctgttggtggtatcatcagaggctcttgttttctttgcatcttgtgtgtttgttggtaactactggttgcattcgctgatgtgttgtttgttgcgattcttgatccagaagagcatcgcaggttcagaagctttacgttggatttctcctacccaagggttccaagcagcgtaaggaatgcatggccagcaattcaaccaggcgatcggatctccggtggtatccagtggcacaggaacgtagctcctcatggtcactctagtgcagtggcgggatatggtgccaacacatacagcggccaaggtagctcttcttcagggccaccggtgttcgctggcccaaatctccctccaggtggatgtctcgcaggggtcggtgccgccaccgactcgagctgtgctctctctcttctgtcaacccagccatgggatactactacccacagtgccgctgccagccacaaccaggctgcagccatgtccactaccaccagctttgatggcaatcctgtggcaccctccgccatggcgggtagctacatggcaccaagcccctggacaggttctcggggccatgagggtggtggtcggagcgtggcgcaccagctaccacatgaagtctcacttgatgaggtgcaccctggtcctagccatcatgcccacttctccggtgagcttgagcttgctctgcaggggaacggtccagccccagcaccacgcatcgatcctgggtccggcagcaccttcgaccaaaccagcaacacgatggattggtctctgtagaggctgttccagctgccatcgatctgtcgtcccgcaaggcgagtcatggaactgaagaacctcatgctgcctgcccttattttgtgttcaaattttcctttccagtatggaaaggaaattctaaggtgactggcgattaatctccctgtgatgaataataatgcgcgcccttgaactcaattaattgctgtgccgcatccatctatgtaactctccatgaatttttaagtatcagtgttaatgctgt</dnaseqindica> |
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