OrfB

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Annotated Information

Function

Please input function information here. The editing of the orfB gene ~1.1 kb transcript co-segregated with fertility restoring alleles in a segregating population of F2 progeny of restored hybrid F1 plants.

Expression

Please input expression information here. Editing of the orfB transcripts (a) The fertile line Mitochondrial RNA editing of the orfB transcript was assessed in the fertile rice line. Fourteen cDNA clones obtained from cDNA library of fertile rice line were sequenced. Determination of the orfB cDNA sequence from overlapping clones from the cDNA library showed four C®T conversions within the coding region relative to the orfB genomic sequence.

Evolution

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You can also add sub-section(s) at will. orfB transcripts of the sterile line have identical 3’ ends with that of transcripts from the fertile lines The basis of the observed differences in the orfB gene transcripts between the sterile and fertile lines was determined using 3’ RACE. The forward primer OGSP1 (Figure 4) annealed 180 bp downstream of the initiation codon in the coding region of the orfB gene. In both the fertile and sterile rice lines, one amplified band of ~400 bp was obtained

Labs working on this gene

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References

Please input cited references here. 1. Eckardt NA: Cytoplasmic male sterility and fertility restoration. Plant Cell 2006, 18:515-517. 2. Hanson MR: Plant mitochondrial mutation and male sterility. Annu Rev Genet 1991, 25:461-486. 3. Hanson MR, Bentolila S: Interactions of mitochondrial and nuclear genes that affect male gametophyte development. Plant Cell 2004, 16:154-169. 4. Schnable PS, Wise RP: The molecular basis of cytoplasmic male sterility and fertility restoration. Trends Plant Sci 1998, 3:175-180. 5. Chase CD, Babay-Laughnan S: Cytoplasmic male sterility and fertility restoration by nuclear genes. Molecular Biology and Biotechnology of Plant Organelles Springer-VerlagDaniell H, Chase CD 2004, 593-622. 6. Gutierres S, Combettes B, De Paepe R: In the Nicotiana sylvestris CMSII mutant, a recombination-mediated change 5’ to the first exon of the mitochondrial nad1 gene is associated with lack of the NADH: ubiquinone oxidoreductase (complex I) NADI subunit. Eur J Biochem 1999, 216:361-370. 7. Unseld M, Marienfeld JR, Brandt P, Brennicke A: The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides. Nature Genet 1997, 15:57-61.

Gene Name	orfB
Description	ORFB protein
Version	GeneID:6450152
Length	468 bp
Definition	Oryza sativa Japonica Group orfB, Mitochondrion gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Mitochondrion
Location	Mitochondrion:53424..53891
Sequence Coding Region	53424..53891
Genome Context	<gbrowseImage3> name=NC_011033:53424..53891 source=Rice_Japonica_Mitochondrion preset=GeneLocation </gbrowseImage3>
Gene Structure (RNA Editing)	<gbrowseImage2> name=NC_011033:53424..53891 source=Rice_Japonica_Mitochondrion preset=GeneLocation </gbrowseImage2>
Protein Sequence	<aaseq>MPQLDKLTYFSQFFWLCLFFFSFYILLLNNNNGILGISRILKLRNQLLSHRGNKIRFKDPKNLEDILRKGFSTGLSYMYSSLSEVSQWCKTVDYLGKRRKITLISDFGEISGSRGMERQILYLISKSSYNTSSSRITCWKKIMLTHVLHGQGSII</aaseq>
Gene Sequence	<dnaseqindica>1..468#atgcctcaacttgataaattgacttatttctcacaattcttctggttatgccttttcctctttagtttttatattctcttattaaataataataatggaatacttggaattagcagaattctcaaactacggaaccaactgctttcgcaccgggggaacaagatccgtttcaaggaccctaagaatttggaagatatctcgagaaaaggttttagcaccggtctctcatatatgtactccagtttatccgaagtatcccaatggtgtaagaccgtcgactatttgggaaaaaggaggaaaatcactctgatctcagatttcggagaaataagtggctcacgaggaatggagagacagattctctatttgatctcgaagtcctcatataacacttcttccagtcggatcacttgttggaaaaaaataatgctcacacatgttccacacgggcaaggaagcataatctaa</dnaseqindica>
External Link(s)	NCBI Gene:orfB