Difference between revisions of "Os02g0726700"
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| − | + | OsbHLH133 is a regulator of iron distribution between roots and shoots in Oryza sativa. | |
==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
| − | + | OsbHLH133 was found to be induced by Fe-deficiency conditions in Oryza sativa. Insertional inactivation of OsbHLH133 (bhlh133) resulted in growth retardation, with enhanced Fe concentration seen in shoots, and reduced Fe concentration in roots. Overexpression of OsbHLH133 had the opposite effect, that is resulted in an enhanced Fe concentration in roots and reduced Fe concentration in shoots and also in xylem sap. Microarray analysis showed that some of the genes encoding Fe-related functions were up-regulated under Fe-sufficient conditions, in bhlh133 mutant plants compared to wild-type plants. Significant differential expression of a number of signalling pathways, including calcium signalling, was also seen in bhlh133 plants compared to wild-type plants, independent of Fe conditions. | |
| + | |||
| + | 1.RT-PCR analyses showed that the transcript abundance of OsbHLH133 was significantly induced by Fe deficiency in rice roots and slightly in leaves. | ||
| + | |||
===Expression=== | ===Expression=== | ||
Revision as of 13:15, 6 June 2014
OsbHLH133 is a regulator of iron distribution between roots and shoots in Oryza sativa.
Contents
Annotated Information
Function
OsbHLH133 was found to be induced by Fe-deficiency conditions in Oryza sativa. Insertional inactivation of OsbHLH133 (bhlh133) resulted in growth retardation, with enhanced Fe concentration seen in shoots, and reduced Fe concentration in roots. Overexpression of OsbHLH133 had the opposite effect, that is resulted in an enhanced Fe concentration in roots and reduced Fe concentration in shoots and also in xylem sap. Microarray analysis showed that some of the genes encoding Fe-related functions were up-regulated under Fe-sufficient conditions, in bhlh133 mutant plants compared to wild-type plants. Significant differential expression of a number of signalling pathways, including calcium signalling, was also seen in bhlh133 plants compared to wild-type plants, independent of Fe conditions.
1.RT-PCR analyses showed that the transcript abundance of OsbHLH133 was significantly induced by Fe deficiency in rice roots and slightly in leaves.
Expression
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Evolution
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Labs working on this gene
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References
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Structured Information
| Gene Name |
Os02g0726700 |
|---|---|
| Description |
Basic helix-loop-helix dimerisation region bHLH domain containing protein |
| Version |
NM_001054528.1 GI:115448426 GeneID:4330593 |
| Length |
1936 bp |
| Definition |
Oryza sativa Japonica Group Os02g0726700, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 2:31120147..31122082 |
| Sequence Coding Region |
31120423..31120583,31120671..31120848,31120955..31120993,31121072..31121167,31121247..31121314 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008395:31120147..31122082 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008395:31120147..31122082 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggagatccgcagatcactgaggccactggacctggccaagcagcgcggccgcagcagcggcaatggcacggcggcggggagtgccgtcgacggcgcgtcgccggcaggttcggactcggaggagcacgtgctaccgggcggcgtcgggacgttcagcatcaggcacgcgtccggtactccgtcgagagaagaggccggtagccatggcggcgtcaggaggagtgcgttcgcatttgcacctgcgcttcatggcgccaggatggagaatgcacatgaaacaggaggaagatctgggagtagggctcaccgtgcgccgtcgacgatgtggcaagattctgccattgatcaaagatccataggccagacaccatatgaagcaacaagagcagagggaagaagtagcgccagcagcgccgatcagggacccagcactccgagatcaaagcattcagcaactgagcagagaaggaggaccaagataaatgacaggcttgaaatacttcgtgaactcctgccacacactgatcagaagagagacaaagcttctttcctttcggaggttattgaatacataaggtttttgcaagagaaagtccaaaagtatgaggaagccgatccagaaaggaatcacgaggattccaaatccatgccatgggcaaaagtctactatagatcatgctggagaaacacaaagaacaccagtcaagtccagggggaagatttgtcaccatccacacaggacatgaacaacgaacagtacggtcccaagcacatatcggcagcacaacctgctctcttcaacacacagagtgtaaccagtacaaccaccagttcctcccacatggcaacaggcacaccacaaaatttggaaaagaacagcacacccagcaatcagccaccatggctgagcatgtcaacaatgcgccaagagtctgaaccaggcaacaaaatgccgaacaagcatgagaaacagacccttcacgatgaaaaccatagtatttctagcgcttactctcaagggtctgttccttaa</cdnaseq> |
| Protein Sequence |
<aaseq>MEIRRSLRPLDLAKQRGRSSGNGTAAGSAVDGASPAGSDSEEHV LPGGVGTFSIRHASGTPSREEAGSHGGVRRSAFAFAPALHGARMENAHETGGRSGSRA HRAPSTMWQDSAIDQRSIGQTPYEATRAEGRSSASSADQGPSTPRSKHSATEQRRRTK INDRLEILRELLPHTDQKRDKASFLSEVIEYIRFLQEKVQKYEEADPERNHEDSKSMP WAKVYYRSCWRNTKNTSQVQGEDLSPSTQDMNNEQYGPKHISAAQPALFNTQSVTSTT TSSSHMATGTPQNLEKNSTPSNQPPWLSMSTMRQESEPGNKMPNKHEKQTLHDENHSI SSAYSQGSVP</aaseq> |
| Gene Sequence |
<dnaseqindica>1500..1660#1235..1412#1090..1128#916..1011#769..836#466..579#1..379#atggagatccgcagatcactgaggccactggacctggccaagcagcgcggccgcagcagcggcaatggcacggcggcggggagtgccgtcgacggcgcgtcgccggcaggttcggactcggaggagcacgtgctaccgggcggcgtcgggacgttcagcatcaggcacgcgtccggtactccgtcgagagaagaggccggtagccatggcggcgtcaggaggagtgcgttcgcatttgcacctgcgcttcatggcgccaggatggagaatgcacatgaaacaggaggaagatctgggagtagggctcaccgtgcgccgtcgacgatgtggcaagattctgccattgatcaaagatccataggccagacaccatatgaaggttagtaccgcagttgtattgttgattctctagtctgtcacttcaattcaggcactgatggatttgatatatacatgtgcactcagcaacaagagcagagggaagaagtagcgccagcagcgccgatcagggacccagcactccgagatcaaagcattcagcaactgagcagagaaggaggaccaagataaatgacaggcaagctagtagaggtcaaacaccattttgaattattttctgcagagcaggaacaaaccaaccatgaagacgtagctagatagctcaacaagatatcatattagcagcatttaattccaagacaatttcatataagtatatggtgcaagaaatgtgttctaaaatgcaattcttcatgctctccttcaggcttgaaatacttcgtgaactcctgccacacactgatcagaagagagacaaagcttctttcctttcggaggtaaacagaatatcaattttggcactgtagtcagcaggctatattgcaatttctcccttgtgaagcattttgtttgcaggttattgaatacataaggtttttgcaagagaaagtccaaaagtatgaggaagccgatccagaaaggaatcacgaggattccaaatccatgccatgggtactatcatagccatgttagcatgttcatgacataattgtgcaacagtattcaccctctatttttttttcttggtaggcaaaagtctactatagatcatgctggagaaacacaaaggtaattccgtaaaataaaaacagcaactgaaagcgaatgcacaattcatcataatcatataccaatgatatgtacccatccttgtttcttgttcatggtctcacagaacaccagtcaagtccagggggaagatttgtcaccatccacacaggacatgaacaacgaacagtacggtcccaagcacatatcggcagcacaacctgctctcttcaacacacagagtgtaaccagtacaaccaccagttcctcccacatggcaacaggcacaccacaaaatttggaaagtaagtccatttcttatcacctttcaacagtcagaattcttgtaattaagaactagatccactaactttaatgtcttttaaacgcagagaacagcacacccagcaatcagccaccatggctgagcatgtcaacaatgcgccaagagtctgaaccaggcaacaaaatgccgaacaagcatgagaaacagacccttcacgatgaaaaccatagtatttctagcgcttactctcaagggtctgttccttaaccatttgttacttcaagactaaagttagcacaatatgtaactaacaggatactacttcaatttgcaacagattattcaacagattgacagaagctcttaaaaagtcaggactagatccatctcaagctaatatagctgtagagatcaacctggccaaacgagcaagagataatactagtgacaactcaaaggtatagagctttacaaagttgatttattcatgatgtttgaacatatagatcgtctgcttaataattattttgtacctttgtcctg</dnaseqindica> |
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