AB013448

Annotated Information

Function

Rice is a good material for studying positional cloning of disease resistance genes because its ratio of physical to genetic distance is small (100 to 300 kb/cM), and abundant genetic information is available. As a first step in cloning, the rice blast resistance gene Pi-b was finely mapped near the terminal region of chromosome 2 using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. Various near-isogenic lines (NILs) developed by the introgression of the indica-derived Pi-b gene into japonica recurrent parents facilitated its rapid and accurate localization and also the F2 analyses. Although Pi-b was introgressed independently from four cultivars, all the donors showed the same RFLP profile for all the nearby probes used, whereas there was some polymorphism among japonica recurrent parents, suggesting a single origin of the resistance gene. Pi-b cosegregated with RAPD (b-1) and RFLP (RZ123) markers, and it was bracketed between flanking RFLP markers at 0.5 cM on the centromeric side, and 1.9 cM on the telomeric side. The centromeric and telomeric limits of the indica regions of NILs BL-1 and BL-7 were closer to Pi-b than the flanking nucleic markers on each side, respectively, providing better (<0.5 and <1.9 cM) delineating markers. A long-range Southern blot with rare-cutter restriction enzymes revealed an 85-kb common band between the two 0-cM markers. These markers provide an excellent environment for the positional cloning of Pi-b.

常规信息

Pib ，水稻稻瘟病抗性基因，来自品种"BL1"，对日本大多数稻瘟病菌小种有抗性，对中国的菌株ZB13 和ZC15 也表现抗病反应。

基因的定位

Pib 基因定位于水稻第2 染色体长臂近末端区域，与RFLP标记RZ123, C379, C2782B 等连锁(Miyamoto et al., 1996; Monna et al., 1997)。 Pib 基因定位于水稻第2 染色体上RFLP 标记S1916 和G7030 之间，遗传距离分别为0.015 cM 和0.045 cM，并与RFLP 标记G7010, G7021 和G7023 共分离(Wang et al., 1999)。对应于日本晴测序图谱的位置(5'-3')在35109965 - 35109117 区间(Rice Genome Annotation Project: TIGR version6)。

基因克隆与生物学功能分析

Pib 基因属于"NBS-LRR"类抗病基因，包含有4个内含子(164 bp, 810 bp, 1340 bp, 308 bp)，全长cDNA由306bp 的5'非翻译区(UTR, untranslated regions)、3753bp 的ORF 和229bp 的3'非翻译区等组成。Pib 编码一个由1251 个氨基酸组成的蛋白产物，该产物包含一个核苷酸结合位点(nucleotide binding site, NBS)和17个富亮氨酸重复序列(leucine-rich repeats, LRRs)，其中，氨基端的NBS 区存在激酶1a, 2 和3a 结构域单位，LRRs 中部有8 个成簇的半胱氨酸残基(Wang et al., 1999)。 Pib 基因会因环境条件的变化而诱导调控，如温度、光照等条件的改变都将影响该基因的表达(Wang et al., 1999)。

分子标记辅助选择育种

利用抗稻瘟病基因Pib 自身序列及其等位的感病基因序列建立的分子标记结合运用，可有效地从水稻种质资源中快速而准确地选择出抗稻瘟病基因Pib，并能在分离世代群体中选择出含抗稻瘟病基因Pib 的纯合单株(刘洋等, 2008)。

Expression

The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.

Evolution

Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.

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Labs working on this gene

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References

1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675

2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14

3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64

4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176

5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13

Structured Information

Gene Name	AB013448
Description	10322 bp DNA linear PLN 15-FEB-2008.
Version	AB013448.1 GI:4521189
Length	10322 bp
Definition	Oryza sativa Japonica Group gene for Pib, complete cds.
Source	Oryza sativa Japonica Group (Japanese rice) ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 2
Location	Chromosome 2:2293..9202
Sequence Coding Region	2293..9202
Expression	GEO Profiles:AB013448
Genome Context	{{{GCID}}}
Gene Structure	{{{GSID}}}
Coding Sequence	{{{CDNA}}}
Protein Sequence	<aaseq>MEATALSVGKSVLNGALGYAKSAFAEEVALQLGIQKDHTFVADE LEMMRSFMMEAHEEQDNSKVVKTWVKQVRDTAYDVEDSLQDFAVHLKRPSWWRFPRTL LERHRVAKQMKELRNKVEDVSQRNVRYHLIKGSAKATINSTEQSSVIATAIFGIDDAR RAAKQDNQRVDLVQLINSEDQDLKVIAVWGTSGDMGQTTIIRMAYENPDVQIRFPCRA WVRVMHPFSPRDFVQSLVNQLHATQGVEALLEKEKTEQDLAKKFNGCVNDRKCLIVLN DLSTIEEWDQIKKCFQKCRKGSRIIVSSTQVEVASLCAGQESQASELKQLSADQTLYA FYDKGSQIIEDSVKPVSISDVAITSTNNHTVAHGEIIDDQSMDADEKKVARKSLTRIR TSVGASEESQLIGREKEISEITHLILNNDSQQVQVISVWGMGGLGKTTLVSGVYQSPR LSDKFDKYVFVTIMRPFILVELLRSLAEQLHKGSSKKEELLENRVSSKKSLASMEDTE LTGQLKRLLEKKSCLIVLDDFSDTSEWDQIKPTLFPLLEKTSRIIVTTRKENIANHCS GKNGNVHNLKVLKHNDALCLLSEKVFEEATYLDDQNNPELVKEAKQILKKCDGLPLAI VVIGGFLANRPKTPEEWRKLNENINAELEMNPELGMIRTVLEKSYDGLPYHLKSCFLY LSIFPEDQIISRRRLVHRWAAEGYSTAAHGKSAIEIANGYFMELKNRSMILPFQQSGS SRKSIDSCKVHDLMRDIAISKSTEENLVFRVEEGCSAYIHGAIRHLAISSNWKGDKSE FEGIVDLSRIRSLSLFGDWKPFFVYGKMRFIRVLDFEGTRGLEYHHLDQIWKLNHLKF LSLRGCYRIDLLPDLLGNLRQLQMLDIRGTYVKALPKTIIKLQKLQYIHAGRKTDYVW EEKHSLMQRCRKVGCICATCCLPLLCEMYGPLHKALARRDAWTFACCVKFPSIMTGVH EEEGAMVPSGIRKLKDLHTLRNINVGRGNAILRDIGMLTGLHKLGVAGINKKNGRAFR LAISNLNKLESLSVSSAGMPGLCGCLDDISSPPENLQSLKLYGSLKTLPEWIKELQHL VKLKLVSTRLLEHDVAMEFLGELPKVEILVISPFKSEEIHFKPPQTGTAFVSLRVLKL AGLWGIKSVKFEEGTMPKLERLQVQGRIENEIGFSGLEFLQNINEVQLSVWFPTDHDR IRAARAAGADYETAWEEEVQEARRKGGELKRKIREQLARNPNQPIIT</aaseq>
Gene Sequence	<dnaseqJaponica>1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341# 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421#3481..3541#3601..3661#3721..3781#3841..3901#3961..4021#4081..4141#4201..4261# cggccgcata atacgactca ctatagggat ctcctctaga gttactttgc cgtgctccat actatcctat tctatattgg attatactat cgatccaacg attcagatta actctatact agtgaagtct acacttatgg tatgggtaat atacatatgt agtatagtat agcataaggg tatttcattt tgcaggttag ccgtttatct gctggtgctc ctcttgctgt agtagtgttg ttggtgttgc tgctgatgac ctaaaatgct tgcatgtttc tatcatgttc tccataatgt agtatcatgt actccatctt ccttgttggt ttttgtccat aatctccacc ttggcagctt gcatcatctt actctcgagc ttgtccacct tgagattcaa ctcctggaac gcggctccca gttcatccac cctcttctcc acggcaggaa tccgtgactc caccgtacgc ttgagatctt ggtactccgc cctggtgcgc tcatcagcct caactcgttt cttctcattc ccttccacaa gttgcagaag gaggtccaac ttcttatcag tctccatggc ctcggatctg gatcaggtac ctactgctct cgctccgaat tccgcgaacc ttagggggca agtttccttt tcgcggtgcc gatccgaaga tcagctccaa tccaccccaa ggaacaattt caccgcagaa tcaagagaat ttgagaagca agagaggctc tgataccaga ttgtcaggat ctcaagaaat cagcaaagaa caacaagaac acacaaggat tcaggcaact agtttggatt gatctgctcc aacccaacag gattgagcct tccgccgcca ccgccaccga gttgccagtt catagttgtc tttctcgagt tcatcttatt tatacagtag tatctcccta ctcacacgac acacacagta gccagctgta caacagatag ctgggctacg caacccactc ggacccatgg taacgaggat tgggctttgg ccctcttgtg ggtcttgctc ttcctggagt agtagtctgt atctcctcct cctggacttc agcttctgct tcatcaggtt ctccttcttc aggttccttc tctccctgtt cagcttctgc ttcatcaggt tctccttctt cagtacccat agtgacaggc aggttcctga caaaattctg ctcgtttgcg accaatggta gtgatcatag ttgcaaccag gagggggggg ggggaaatcg ccgtcccctc cgctcctctc ccgtcgtccc caacgcctcg ttcgcgcatt tcgttgaaca ccatgacggc gccgaattcg cagtgtccgc acatctcctc ctcccccgtc ctctccaaac cccaaaccct atctccaccc ccgaggcagg cgcccccatg cacttgtaag tcgattggat gtcctgtccc agaagacata tcgagcgagg aggcggaggg ggacgaaggg aacatatcga gcgaggaagc ggagggtgga tcggcatccc ccatttcaag gtactatact agtccattat agtagtagtg cttttgcatc ttagaaaaaa aaatatgttc attagccatt gagagcttct gaagttgttg attttgttcc aaccccaact gtgagtttca gttcaggtca tccactgatt ttcactatgc caattctctg aaacaacttt accactgtca catgaacaca ctgaaacagt ttggtgtaga cgtgtagtga agaatgtagc atatatacct tcacttaatt tttcttgcaa ttattggcca ttactagtta tgcgaggtag aagtgttcta aggtactgta tcatttttat gtactaatta attaagttta ataaaaactt ttattatcta aaaataaatg actattacta gctcggtact ccctttattt tatattataa gacgttttga tttttttata tacaactttc tttaagtttg attatactta taaaaaatta gcaaacatat atattttttt tacattaata gtgcaagtga gcacgcttaa atgcattgta cttccttcgt aaaaaaacat caaactttta cggacgaata tggataaatg catatctaaa ttcatcctca ataattgatt ctttttggag gagtacaatt ggttggtgcg ctttgtcctt ggaccctaca ataatgatga ttgtttcttt aatctattga ccttgactta ccacatgggc tatgtttatc ccttcctgaa tcctgagcac tgactaccga ggcaccgagt gtgagcggca acggcggtca gggagcaggc gtggctcgtc ggcgagcggc tacgggcaac ggcgccttgg cgtcaggcat ccgccgtcac tcacctcaag cttgcgggct ctgcgaccac cctctcatag tcataggcca cagaaggtgt agtagtactt catacatttc gagcagtttc tttcagattg tttgtttttg agcttctaat tttgggatgc attagatagt gatgaaagcc tgaattattg gaattttggt gttggtactc acactctcac agtcagaaca tactcctata tattttgcag cacatttgcc ttgtgcgtgc tgttcgtctg ttccactcgt gaacatcaga cgcgaagatt atagattcac ccctgttcac agattcaggt actgccaatt gcctggatga acaccagtcc atttgctctc tttcgcctta caatttttct ctgcattgta ctagcagccg tagctcgaaa gcctcgaata tgattccttt tcaagatttt atatttatgg aatataattc acttttaaga tgccttgatg gtgaaatagt agacatgtga gactccaaat ctcgtcctaa aagagcatgg aggtaaaaaa agaaaaaggt agacatcgct attgtagaca tggagagctg gaatacgatt actttcaaga tattatattc aatgagcatt cattcttaca catatgccac aaaggtaaaa aaaaacagag aaagagagag agaggggaaa gaagccaagt tctttcttct actatcattt aggttgagtt cgtttgttaa ggttcccaac ctacgattcc tcgtttcccg cgtgcacgat tcccaaacta ctaaatggta tgctttttaa aatatttcgt agaaaaattg ctttaaaaaa tcatattaat ttatttttta agttgtttag ctaatactca attaatcatg cattaatttg ccgctccgtt ttagtggaag tcatctgaaa ggatcaaagg aagcaacacc aagtccttat ttcgactccg actctctcac tctcgccatt tattcttttc tttctgttat tttaaaagtt gctactttag cttcagccac gtgaattctt gatatttcat tatttttctc atcaaacaat agcatcttct tctggaaatc gaattcaggg cttatatgtt gcttattctg atatataggt ctgtcacgag gcgtatgatc atcaactctg ccacaaaatc cattcaaaaa tagaacagag caatggaggc gacggcgctg agtgtgggca aatccgtgct gaatggagcg cttggctacg caaaatctgc atttgctgag gaggtggcct tgcagcttgg tatccagaaa gaccacacat ttgttgcaga tgagcttgag atgatgaggt ctttcatgat ggaggcgcac gaggagcaag ataacagcaa ggtggtcaag acttgggtga agcaagtccg tgacactgcc tatgatgttg aggacagcct ccaggatttc gctgttcatc ttaagaggcc atcctggtgg cgatttcctc gtacgctgct cgagcggcac cgtgtggcca agcagatgaa ggagcttagg aacaaggtcg aggatgtcag ccagaggaat gtgcggtacc acctcatcaa gggctctgcc aaggccacca tcaattccac tgagcaatct agcgttattg ctacagccat attcggcatt gacgatgcaa ggcgtgccgc aaagcaggac aatcagagag tggatcttgt ccaactaatc aacagtgagg atcaggacct aaaagtgatc gcggtctggg gaacaagtgg tgatatgggc caaacaacaa taatcaggat ggcttatgag aacccagatg tccaaatcag attcccatgc cgtgcatggg taagggtgat gcatcctttc agtccaagag actttgtcca gagcttggtg aatcagcttc atgcaaccca aggggttgaa gctctgttgg 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341# 4321 agaaagagaa gacagaacaa gatttagcta agaaattcaa tggatgtgtg aatgatagga 4381 agtgtctaat tgtgcttaat gacctatcca ccattgaaga gtgggaccag attaagaaat 4441 gcttccaaaa atgcaggaaa ggaagccgaa tcatagtgtc aagcactcaa gttgaagttg 4501 caagcttatg tgctgggcaa gaaagccaag cctcagagct aaagcaattg tctgctgatc 4561 agacccttta cgcattctac gacaaggtaa tatacttgct cttcaagcat acctctcgat 4621 atcattttta attcagttat gcctttagta atttctaatt caattgtgta taggctagtt 4681 gaagtgcgtg ggagttacca ttccattaga aacacatgac ctaatgcaac taacaagtgc 4741 tcctcctgtt ctctctcatt tgccttttgg gaatgcatgc actcaacatt ttaagattac 4801 agccaaaata tatgtatttg gatttgtcaa aacaaagatg tatgctagaa aaagaaatgg 4861 tctaatacag gtttacaaat aagacaacga tgcaaaaagg gcaactaaaa acatattgat 4921 tccctcatct gccactgcaa ttgccttaaa ttctagtcca ttctactatc tccgtttcat 4981 attataagtc actctagttt ttttccagtc aaacttcttt agtttgacca agtttataca 5041 aaaatttagc aacatatcca acacgaaatt agtttcatta aatgtagcat tgaatatatt 5101 ttgatagtat gtttgttttg tgttgaaaat gctgctatat tttttaaaaa aacttggtca 5161 aacctaaaca agtttgacta ggagaaaagt cgaaacgact tataatatga aatagaggga 5221 gaatgttcga agtttggcta acggtcaatg ctagtgcttt aagtgggtaa gccgcaaatc 5281 caattatagg ccaaaataca tgggtttgtg gcttattttg gctataagtg ggtttcgcgg 5341 gttagccact tacaccccta gtcaatgcta atgaaagtag aagtgatgct attcaaggaa 5401 aatgtattgg ataccgagat tgccttgaat aaagaataaa attgaggtag tagattggat 5461 aatagattga cccacaaaat tgtacaagta tgtaatgtag cacaagtcct ctttgcacaa 5521 ttaaaatttt gaagctccta tttcacaaat aattttgata tggattaatt gatttcatat 5581 ccaattcgca cagtttattg aatttggaga tttatttcct ctatatgtga gagatgattg 5641 taaaatgggc aaatctagca aatgcatcct ctcatccttt ggattaaatg tagtgtactt 5701 atcccattat tttaaagtta aattaataca tattttattg aacagtcaga tatacgtttt 5761 tcaaaatagg atccaaaact aaggtttata ctagactgca aattaatgaa aggaattatc 5821 attattgttt tgtatacttt catgaccgaa aacaaggcta aacactatcc atgtatgaaa 5881 atttaaggct aaaagttgtt cttaatcatt gctccctttt gtttagggtt cccaaattat 5941 agaggattca gtgaagccag tgtctatctc ggatgtggcc atcacaagta caaacaatca 6001 tacagtggcc catggtgaga ttatagatga tcaatcaatg gatgctgatg agaagaaggt 6061 ggctagaaag agtcttactc gcattaggac aagtgttggt gcttcggagg aatcacaact 6121 tattgggcga gagaaagaaa tatctgaaat aacacactta attttaaaca atgatagcca 6181 gcaggttcag gtgatctctg tgtggggaat gggtggcctt ggaaaaacca ccctagtaag 6241 cggtgtttat caaagcccaa ggctgagtga taagtttgac aagtatgttt ttgtcacaat 6301 catgcgtcct ttcattcttg tagagctcct taggagtttg gctgagcaac tacataaagg 6361 atcttctaag aaggaagaac tgttagaaaa tagagtcagc agtaagaaat cactagcatc 6421 gatggaggat accgagttga ctgggcagtt gaaaaggctt ttagaaaaga aaagttgctt 6481 gattgttcta gatgatttct cagatacctc agaatgggac cagataaaac caacgttatt 6541 ccccctgttg gaaaagacaa gccgaataat tgtgactaca agaaaagaga atattgccaa 6601 ccattgctca gggaaaaatg gaaatgtgca caaccttaaa gttcttaaac ataatgatgc 6661 attgtgcctc ttgagtgaga aggtaatata agtgtgctcc atttttcttg gtttgatatt 6721 cttttaatca tttgagttat ccaatcaaga tgatatttgt gcatgcagaa atagcatata 6781 ctagattcat atacaactta atctgttctc acaacaatag caatgcagtt cctaaaatga 6841 cctgcattgg atggacgtta gatgtgactt tgtttttgta tgtaatggtg gccttcattc 6901 cttagtttta atagtaaaga cgtatttcta aatttaattt tttttgtttt actttagagc 6961 acaataaagc ttaaattgta tcaatgtcag gtatttgagg aggctacata tttggatgat 7021 cagaacaatc cagagttggt taaagaagca aaacaaatcc taaagaagtg cgatggactg 7081 ccccttgcaa tagttgtcat aggtggattc ttggcaaacc gaccaaagac cccagaagag 7141 tggagaaaat tgaacgagaa tatcaatgct gagttggaaa tgaatccaga gcttggaatg 7201 ataagaaccg tccttgaaaa aagctatgat ggtttaccat accatctcaa gtcatgtttt 7261 ttatatctgt ccattttccc tgaagaccag atcattagtc gaaggcgttt ggtgcatcgt 7321 tgggcagcag aaggttactc aactgcagca catgggaaat ctgccattga aatagctaac 7381 ggctacttca tggaactcaa gaatagaagc atgattttac cattccagca atcaggtagc 7441 agcaggaaat caattgactc ttgcaaagtc catgatctca tgcgtgacat cgccatctca 7501 aagtcaacgg aggaaaacct tgtttttagg gtggaggaag gctgcagcgc gtacatacat 7561 ggtgcaattc gtcatcttgc tataagtagc aactggaagg gagataagag tgaattcgag 7621 ggcatagtgg acctgtcccg aatacgatcg ttatctctgt ttggggattg gaagccattt 7681 tttgtttatg gcaagatgag gtttatacga gtgcttgact ttgaagggac tagaggtcta 7741 gaatatcatc accttgatca gatttggaag cttaatcacc taaaattcct ttctctacga 7801 ggatgctatc gtattgatct actgccagat ttactgggca acctgaggca actccagatg 7861 ctagacatca gaggtacata tgtaaaggct ttgccaaaaa ccatcatcaa gcttcagaag 7921 ctacagtaca ttcatgctgg gcgcaaaaca gactatgtat gggaggaaaa gcatagttta 7981 atgcagaggt gtcgtaaggt gggatgtata tgtgcaacat gttgcctccc tcttctttgc 8041 gaaatgtatg gccctctcca taaggcccta gcccggcgtg atgcgtggac tttcgcttgc 8101 tgcgtgaaat tcccatctat catgacggga gtacatgaag aggaaggcgc tatggtgcca 8161 agtgggatta gaaaactgaa agacttgcac acactaagga acataaatgt cggaagggga 8221 aatgccatcc tacgagatat cggaatgctc acaggattac acaagttagg agtggctggc 8281 atcaacaaga agaatggacg agcgtttcgc ttggccattt ccaacctcaa caagctggaa 8341 tcactgtctg tgagttcagc agggatgccg ggcttgtgtg gttgcttgga tgatatatcc 8401 tcgcctccgg aaaacctaca gagcctcaag ctgtacggca gtttgaaaac gttgccggaa 8461 tggatcaagg agctccagca tctcgtgaag ttaaaactag tgagtactag gctattggag 8521 cacgacgttg ctatggaatt ccttggggaa ctaccgaagg tggaaattct agttatttca 8581 ccgtttaaga gtgaagaaat tcatttcaag cctccgcaga ctgggactgc ttttgtaagc 8641 ctcagggtgc tcaagcttgc aggattatgg ggcatcaaat cagtgaagtt tgaggaagga 8701 acaatgccca aacttgagag gctgcaggtc caagggcgaa tagaaaatga aattggcttt 8761 tctgggttag agtttctcca aaacatcaac gaagtccagc tcagtgtttg gtttcccacg 8821 gatcatgata ggataagagc cgcgcgcgcc gcgggcgctg attatgagac tgcctgggag 8881 gaagaggtac aggaagcaag gcgcaaggga ggtgaactga agaggaaaat ccgagaacag 8941 cttgctcgga atccaaacca acccatcatt acctgagctc ctttggagtt actttgccgt 9001 gctccatact atcctacaag tgagatcctc tgcagtactg catgctcact gacatgtgga 9061 cccgaggggc tgtggggccc acatgtcagt gagcagtact gtgcagtact gcagaggacc 9121 tgcatccact atcctatatt ataatggatt gtactatcga tccaactatt cagattaact 9181 ctatactagt gaacttattt ttttttgccg ggccggcaaa tagctggtcg atgtatatta 9241 agaataagaa agggaatgta caagatagcg cggtgcgtca atgcaccacc attacagacg 9301 taaaaggaaa gctaaaatct cacagaatga gttgctacag agtgacacat ggggctaaca 9361 agacctgcag ctatccaagt ctcccattca tcccccatgg cagaacagaa ctggggaacc 9421 gttgccgcga tcccttcaaa cacccttgcg tttcgctctt tcgaaatcaa ccaggttaca 9481 aggatcaccc ttgcatcgaa cgttttgcgg tcaaccttag caacagattt ccgggctgca 9541 agccaccaat cagcaaaatc agccgacgat gaggagcacg aaaggaccag gcgtgtgcgc 9601 acctgacctc aaatctcctg ggtgtaagag cagcccacga agatgtgctg gcaggtttcc 9661 ccgtcattgg agcagaaata gcacaccgga gcaagcttcc atctgtgacg ttgtagattg 9721 ttggcagtga ggcaagcatt gcgctcggcg agaaacataa agaacttaca tctcgccggg 9781 gcaagagact tccaaataat ggtatacata tgtagtatat agtatagtat agtatagtat 9841 aagggtattc attttgcagg ttagcggtta tctgctgctg ttcctcctgc tgcggcgtgc 9901 tggagtagtg ttgttggtgg tggtgctgat gacctaaaat gcttgcttgt ttctatcaag 9961 ttctccagaa tgtagtatgt actgcatctt gttgattttt gtccataaac ggattgcatt 10021 atctgtatat gacccaatca acaataaacg gtgttgcatt ttgttcctaa aagctcttag 10081 agtctgacca gttatctctg tacgcatctt catgctgttc tttgggcact ggtcatggtt 10141 aaatcacagt tcaccgaaac ttattttctg tagacttatt ctgaaatact gagaaattga 10201 aatgtagtaa ctattgtctg tagactgctt tctcgttttt cttttgcggt cgccatctcc 10261 agtcagtatc tacagaagaa gagccaatgc agcctattgt cctttttttg ccgggtcggc 10321 cg</dnaseqJaponica>
External Link(s)	NCBI Gene:AB013448, [1]