Os03g0315400

Its gene name OsMYB2, is a R2R3-type MYB gene, expression a MYB-type transcription factor plays a important role in the tolerance to abiotic stress of plants.

Annotated Information

Function

1、Overexpression of OsMYB2 enhanced tolerance to salt cold, and dehydration stress（figure 1）.

The involvement of OsMYB2 in salt, cold, and osmotic stress was investigated by exposing wild-type and the transgenic plants grown in hydroponic solution with NaCl, low temperature, and PEG. There was no difference between transgenic and wild-type plants when grown under normal, non-stressed conditions in hydroponic solution (A). Phenotypically, most OsMYB2-overexpressing seedlings remained green and showed continuous growth, whereas both wild-type and RNAi seedlings showed severe leaf rolling and wilting after exposure to the salt, cold, and osmotic stress (B-D).In addition, the two transgenic rice lines overexpressing OsMYB2 grown in soil also exhibited greater tolerance to NaCl, cold, and drought stress than wild-type and RNAi plants（data not shown）

WT:wildtype; OE2 and OE3: OsMYB2 overexpression strain; Ri1 and Ri3: OsMYB2 RNAi strain;

2、Overexpression of OsMYB2 altered sensitivity of seed germination and growth to salt stress and ABA(figure 2).

This study also determined the survival rate for wild-type and transgenic plants grown in both hydroponic solution and soil challenged with salt, cold, and osmotic stress.the survival rate of overexpressing lines was significantly higher than that of wild-type and RNAi seedlings when exposed to salt stress (200 mM NaCl for 2 d), cold stress (5 �C for 3 d), and osmotic stress (20% PEG6000 for 2 d). The survival rates of the two overexpressing lines higher than those of wild-type and RNAi plants were also observed when rice seedlings grown in soil were challenged by salt, cold, and drought stress（D-F）

3、OsMYB2-overexpression plants accumulated greater amount of proline and soluble sugars(figure 3).

4、OsMYB2-overexpression plants accumulated less H2O2 and MDA under salt stress(figure 4).

5、OsMYB2-overexpression plants affect some genes' expression profiles. Some genes upregulation can contribute to enhanced tolerance of plants to salt stress and some may involved in stress response.

Expression

OsMYB2 was detected in roots,shoots,leaves, and flowers, but it was specifically located in the nucleus(figure 5)

a、b、c: localization of GFP ; e、d、f:localization of GFP-OsMYB2

The response of OsMYB2 expression to salt, cold, and dehydration stress was monitored by real-time RT-PCR. An increase in the OsMYB2 transcript was observed after 30 min of exposure to salt stress. The salt stress-induced increase in the OsMYB2 transcript peaked after 5 h of salt stress, and thereafter the transcript declined gradually under salt stress . A similar increase in the OsMYB2 transcript was also observed when rice seedlings were exposed to low temperature (2℃) or osmotic stress (20% PEG) . In addition, treatment of rice seedlings with ABA also led to an increase in expression of OsMYB2. In contrast, exogenous application of salicylic acid reduced the expression of OsMYB2, while no effect of indoleacetic acid and brassinosteroids on the OsMYB2 transcript was observed. OsMYB2 was detected in roots, shoots, leaves, and flowers under non-stressed conditions, with the expression being greatest in leaves, followed by roots and shoots . The strong induction of this gene by abiotic stress prompted this study to check its promoter sequence (1500 bp upstream from the transcription start site) by searching the promoter sequence against the PLACE database [(http://www.dna.affrc.go.jp/PLACE/)]. The promoter of OsMYB2 contains stress-responsive related cis-elements, such as ABRE and MYB and MYC recognition sites

Evolution

Phylogenetic tree of MYB proteins(figure 6).

OsMYB2 representative by LOC_Os3g20090 is belong to C12, and of which are involved in stress response.

Labs working on this gene

1 State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, PR China

2 Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China

References

A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice.An Yang1,2, Xiaoyan Dai1 and Wen-Hao Zhang*,1.Journal of Experimental Botany, Vol. 63, No. 7, pp. 2541–2556, 2012.

Structured Information

Gene Name	Os03g0315400
Description	Similar to Typical P-type R2R3 Myb protein (Fragment)
Version	NM_001056472.1 GI:115452672 GeneID:4332651
Length	2127 bp
Definition	Oryza sativa Japonica Group Os03g0315400, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 3
Location	Chromosome 3:11376759..11378885
Sequence Coding Region	11377201..11378033,11378520..11378676
Expression	GEO Profiles:Os03g0315400
Genome Context	<gbrowseImage1> name=NC_008396:11376759..11378885 source=RiceChromosome03 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008396:11376759..11378885 source=RiceChromosome03 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atggacatggcgcacgagagggacgcgagcagcgaggaggaggtgatgggcggcgacctgcgtcgcgggccgtggacggtggaggaggacctcctgctcgtcaactacatcgccgcgcacggcgagggccgctggaactcgctcgcccgatcagcagggctgaaacgcacaggcaagagctgccggctccggtggctgaactacctccgccccgacctccggcgaggcaacatcacgccgcaggagcagctgctcatcctggagctgcactcgcggtggggaaaccgctggtccaagatcgcgcagcacctcccgggacgcaccgacaacgagatcaagaactactggcgcacgcgggtgcagaagcacgccaagcagctcaagtgcgacgtcaacagccagcagttcaaggacgtcatgcgctacctctggatgccccgcctcgtcgagcgcatccaggccgccgccgccgggcagcagcagcagcaggaaggcggcaccgacacgccgcccctgtcgtggcagcacggcggctccgacgggctctacgagtcgccggagctcccggcgcccgatgccagctgctggccagccgagtactgcgcggcggccggcggcgcgcagtcgggcggcacgcctgcaccggagctgtcgagcaccacggccgggtcgtcgtcgctgtccacggactccggcgccggggcgcagcccagctggcccacgcaggccgacggcgccgagtggttcaccaccgcctgcgacgcctccagcgccaccggcggcgtggccatgcgcgacacggagctggagctggcccagccgccgtgccagggcgggcagacgtggacgacgtccgagtcgtcgctgcctggcctcaccttccccgacctcgccgtcgcggacttcgagatcggcggcttcgacgtcgatagcttctggacgagcatggaggacgaccagctgtggtgccccacccaggccgccgtgtga</cdnaseq>
Protein Sequence	<aaseq>MDMAHERDASSEEEVMGGDLRRGPWTVEEDLLLVNYIAAHGEGR WNSLARSAGLKRTGKSCRLRWLNYLRPDLRRGNITPQEQLLILELHSRWGNRWSKIAQ HLPGRTDNEIKNYWRTRVQKHAKQLKCDVNSQQFKDVMRYLWMPRLVERIQAAAAGQQ QQQEGGTDTPPLSWQHGGSDGLYESPELPAPDASCWPAEYCAAAGGAQSGGTPAPELS STTAGSSSLSTDSGAGAQPSWPTQADGAEWFTTACDASSATGGVAMRDTELELAQPPC QGGQTWTTSESSLPGLTFPDLAVADFEIGGFDVDSFWTSMEDDQLWCPTQAAV</aaseq>
Gene Sequence	<dnaseqindica>853..1685#210..366#agtttcatcgcagcacacatccatccatccatccatctatccagagagcacagcaacggcgcatatatagtacccctctaccaaagcacaacaaccagaatctcctgagctcgatctagctactagcttgatctatccgatcaatcgactggcccgcgaggatcgatcgagactcgaaagggagggattttgatccggatcggtcgacgatggacatggcgcacgagagggacgcgagcagcgaggaggaggtgatgggcggcgacctgcgtcgcgggccgtggacggtggaggaggacctcctgctcgtcaactacatcgccgcgcacggcgagggccgctggaactcgctcgcccgatcagcaggtaggatcgcgatctcgatcgatcgagctcccaattccaccataaattacatgcacgcacacatcgatcaattccatgagctgagtgtccgtacatgcttagcctgatgagcaattataggacgcaaagtaccagtcgctcttgcatctacatgctgatttctggaaacacagatagctacgagtctaccctgagttctctagcagccaaccagctaagctagaagctatagtgagtgagagggaaaggggagagaatttttagtggttagcagcgtagctagcattgttcatagggatatttttagttgctcatccctcccccgttacatcatccatcctgccgtcgtcgtatgcgtataactgcattagtatataattgattagttgctgcatactatgttttagcaatggtgtactacatgtgaatattttgatgtgacgtgaagagaaaaattaatcttggtttttggttgttgtcatgcagggctgaaacgcacaggcaagagctgccggctccggtggctgaactacctccgccccgacctccggcgaggcaacatcacgccgcaggagcagctgctcatcctggagctgcactcgcggtggggaaaccgctggtccaagatcgcgcagcacctcccgggacgcaccgacaacgagatcaagaactactggcgcacgcgggtgcagaagcacgccaagcagctcaagtgcgacgtcaacagccagcagttcaaggacgtcatgcgctacctctggatgccccgcctcgtcgagcgcatccaggccgccgccgccgggcagcagcagcagcaggaaggcggcaccgacacgccgcccctgtcgtggcagcacggcggctccgacgggctctacgagtcgccggagctcccggcgcccgatgccagctgctggccagccgagtactgcgcggcggccggcggcgcgcagtcgggcggcacgcctgcaccggagctgtcgagcaccacggccgggtcgtcgtcgctgtccacggactccggcgccggggcgcagcccagctggcccacgcaggccgacggcgccgagtggttcaccaccgcctgcgacgcctccagcgccaccggcggcgtggccatgcgcgacacggagctggagctggcccagccgccgtgccagggcgggcagacgtggacgacgtccgagtcgtcgctgcctggcctcaccttccccgacctcgccgtcgcggacttcgagatcggcggcttcgacgtcgatagcttctggacgagcatggaggacgaccagctgtggtgccccacccaggccgccgtgtgaaaagtcagcacggccgccatgggaatccgccgcggcgagcgagcgcgcgcgcgcgcgacacccgcgggtgcacaaccgccggggacgcgtagcggagcggagaagcggattagaagaaggagagaagctatctgggggattagaacaagattaatcgcctcacgatgccatttttggactcctagctcccagactattctaactccagttcttttccgtttctttctccttttttacttcctagggtaaaaaaaaagaagttaaagtgtagccgttatactagtgttgatgctgctgttactaaagtttgtccgttaaattttactcattctttttgagtaaatacagtactgccactactgtatgtacgagctgaactctgtaggattgacaggattactgtacacttctagaaaccggtaataaaagcaaagctccgacg</dnaseqindica>
External Link(s)	NCBI Gene:Os03g0315400, RefSeq:Os03g0315400