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| | ===Expression=== | | ===Expression=== |
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| − | Spatial pattern of OsIRO2 expression. During germination, OsIRO2 expression was detected in embryos(Fig.1). OsIRO2 expression in vegetative tissues was restricted almost exclusively to vascular bundles of roots(Fig.2) and leaves(Fig.3), and to the root exodermis under Fe-sufficient conditions, and expanded to all tissues of roots and leaves in responseto Fe deficiency. OsIRO2 expression was also detected inflowers and developing seeds(fi.4). Plants overexpressing OsIRO2 grew better, and OsIRO2-repressed plants showed poor growth compared to non-transformant rice after germination.[1]<ref name="OsIRO2 is responsible for iron utilization in rice and improves"> | + | Spatial pattern of OsIRO2 expression. During germination, OsIRO2 expression was detected in embryos(Fig.1). OsIRO2 expression in vegetative tissues was restricted almost exclusively to vascular bundles of roots(Fig.2) and leaves(Fig.3), and to the root exodermis under Fe-sufficient conditions, and expanded to all tissues of roots and leaves in responseto Fe deficiency. OsIRO2 expression was also detected inflowers and developing seeds(Fig.4). Plants overexpressing OsIRO2 grew better, and OsIRO2-repressed plants showed poor growth compared to non-transformant rice after germination.[1] |
| | [[File:D 1.JPG]] | | [[File:D 1.JPG]] |
| | [[File:D 2.JPG]] | | [[File:D 2.JPG]] |
| | [[File:D 3.JPG]] | | [[File:D 3.JPG]] |
| | [[File:D 4.JPG]] | | [[File:D 4.JPG]] |
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| − | ===Evolution===
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| − | Please input evolution information here.
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| − | You can also add sub-section(s) at will.
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| − | ==Labs working on this gene==
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| − | Institute of Crop Science, Chinese Academy of Agricultural Sciences
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| − | ==References==
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| − | Please input cited references here.
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| − | 1. Yuko Ogo;Reiko Nakanishi Itai;Hiromi Nakanishi;Haruhiko Inoue;Takanori Kobayashi;Motofumi Suzuki;Michiko Takahashi;Satoshi Mori;Naoko K. Nishizawa. Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants. Journal of Experimental Botany, 2006, 57(11): 2867-2878.
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| − | 2. Yuko Ogo;Reiko Nakanishi Itai;Hiromi Nakanishi;Takanori Kobayashi;Michiko Takahashi;Satoshi Mori;Naoko K. Nishizawa. The rice bHLH protein OsIRO2 is an essential regulator of the genes involved in Fe uptake under Fe-deficient conditions. The Plant Journal, 2007, 51(3): 366-377.
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| − | ==Structured Information==
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| − | {{JaponicaGene|
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| − | GeneName = Os01g0952800|
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| − | Description = Basic helix-loop-helix dimerisation region bHLH domain containing protein|
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| − | Version = NM_001051959.1 GI:115442288 GeneID:4325750|
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| − | Length = 6650 bp|
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| − | Definition = Oryza sativa Japonica Group Os01g0952800, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| − |
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| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
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| − | AP = Chromosome 1:43766291..43772940|
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| − | CDS = 43770559..43770642,43771987..43772343,43772434..43772736|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008394:43766291..43772940
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| − | source=RiceChromosome01
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008394:43766291..43772940
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| − | source=RiceChromosome01
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>atggagcagctgttcgtcgacgacccagccttcgcgagcagcatgtcgtcgcttgaggcggacatcttctccggcgccggccagctgccgtcctcgccgtggctggacctagacctcgacgacgatgtccaagacctctccatggcgccgacgacggcgaacgcggtgtcctccggctacggctccggcggatccggctcccacaggaagctcagccacaacgcctacgagcgcgaccgccggaagcagctcaacgagctctactcctccctccgcgctctcctccccgacgccgatcacactaagctgagcatcccgacgacggtgtctcgcgtgctcaagtacatacccgagctgcagaagcaggtggagaatctggagaggaagaagaaggagctgacgacgacgagcaccaccaactgcaaaccaggagtgttggggagccagctgatgagcgagggcatggctcccatcgtttcggctacctgcatcaatgacatggagatcatggttcaggtcagcttgttgagcaatgtggcgggttcagttcttcctctctccaagtgtatcaaagtactggagaacgaaggtcttcacttcatcagttcatcgacttcctccggatttgggaacaggacattctacagtatccatcttcagagaagtgaaggaacgatcaacgaggagtgcccagcattttgtgaaaggttggagaaagtcgtcaggaacaaagcaaagctttaa</cdnaseq>|
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| − | AA = <aaseq>MEQLFVDDPAFASSMSSLEADIFSGAGQLPSSPWLDLDLDDDVQ DLSMAPTTANAVSSGYGSGGSGSHRKLSHNAYERDRRKQLNELYSSLRALLPDADHTK LSIPTTVSRVLKYIPELQKQVENLERKKKELTTTSTTNCKPGVLGSQLMSEGMAPIVS ATCINDMEIMVQVSLLSNVAGSVLPLSKCIKVLENEGLHFISSSTSSGFGNRTFYSIH LQRSEGTINEECPAFCERLEKVVRNKAKL</aaseq>|
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| − | DNA = <dnaseqindica>2299..2382#598..954#205..507#gctggcctaagctaactcaatcaaacctccctagcttggcagaatagttaatgttaatcacctgattaaaattagattagtcccctcctacccagctaactactgcttctatttcacggagaaaaatgtcttcctaggctttctctttcagctcgatcgatccacagctcagctagctctctctctctctagcgacaagcaagcatggagcagctgttcgtcgacgacccagccttcgcgagcagcatgtcgtcgcttgaggcggacatcttctccggcgccggccagctgccgtcctcgccgtggctggacctagacctcgacgacgatgtccaagacctctccatggcgccgacgacggcgaacgcggtgtcctccggctacggctccggcggatccggctcccacaggaagctcagccacaacgcctacgagcgcgaccgccggaagcagctcaacgagctctactcctccctccgcgctctcctccccgacgccgatcacactgtaatttaatttacacaaaaccaatcaaatccagtacaccaaattaaccatgcatatgcatgagatcttgatgtgattttgctgcagaagaagctgagcatcccgacgacggtgtctcgcgtgctcaagtacatacccgagctgcagaagcaggtggagaatctggagaggaagaagaaggagctgacgacgacgagcaccaccaactgcaaaccaggagtgttggggagccagctgatgagcgagggcatggctcccatcgtttcggctacctgcatcaatgacatggagatcatggttcaggtcagcttgttgagcaatgtggcgggttcagttcttcctctctccaagtgtatcaaagtactggagaacgaaggtcttcacttcatcagttcatcgacttcctccggatttgggaacaggacattctacagtatccatcttcaggtacacattatacacacagctctatgctagaacgtttaattaatatttcttatggtaatatatttttgaaaagaaaatccctctatactatctccattctaaaatataaaaacatagagcccggacagactgtctatccagatttgtagtacgagtaattaaatgtctcatacgattctattaggttttttatattttagaatggatggagtattttcgatcaacaataacctagcatcaaatcaaggactagagtcaggttaagatccgggtaaattagataaagggtgctatagtacaatctagtgatactactactctgccttaaaatagtttctttttagctatttactcgtatatataccactgttcaagttcataactaaacatataggttttttaagatgaaggtagtagtaaatatgagtaaattttatacaactatgagtatattgaataatattatagaactatacttttatatagcacactgtcataaaattagagacttaatactacacatttaaaccacaataacacgaactatatagttgtatatatggcacaaatctagtatagaatatatcatagaactacatattttaataagccatggatctagtaacacaaattacagatttaaatagtggtaaattttattaaaagttatatatattctaaaatttctatcgaaacaacaatgttagctagggatctactatttacaaattacaatatactattttgcaacaaatttgatgctaggtccatagtttttcataggtggtttaatcttgcaaaattgaaaaaacttgtggcaaatttgcaaatgcccctttaatctaccgtttatgctcatagtttgatgttcaatccataattatgtaatattttgcatagtaacagatgagagaaattataagtgaataagatgaaattctactataatcatgagagatatagtagtccctgaatcgtgtgtggaattcatcagctagttgtttttaagatttcaattagagctgaccttcttatgataaaaaccaatgtttcttagtcacaaatctcaaaatatacttgttaaatcgaagatatatgaatcacatgataattgtaaaaatatatgtagcgttactcatgatactaatacttgagataaccctctaaaaatacgggatattacatatgaaagtttctccttgtttgggttaaaattgacagtagagcatgtttgtgtgtatatatagcgtacaattagaccgttatttagttgtcatagaagttcggtgttctgaaaattactcacagttttcatttatatatatgtatctaatgttgaaacagagaagtgaaggaacgatcaacgaggagtgcccagcattttgtgaaaggttggagaaagtcgtcaggaacaaagcaaagctttaagcactagtaccgcgcgcgcgtgcctatgataatcctgaattaatcaagggccaaaatctaggtaaaaatgaccatgcgcagttgttaaatatagcatttggttagctgataataatgatatataagtagtgtgctcgatttatattaactaatttctatgacatatatactatgttgctcgatgtagcttttgtacatatttcatgcatgactctcagtgtattaattaattatattatgagatttgcaaaatgttaatttttgcaatgcttaacgttgctggaggaaaatcaaaccttaagaatatattactttcgtccgctaatataagggattttgacattttacttgtactgtttggccattcgtgttattcaaaaaaatttagaattattatttattttatttgtgacttactttattatccaaagtactttaagcacaacttctctttttttatatttgcacaatttttttaaataagacgagtggtcaaacagtgcaaacaaaatgtcaaaaatcccttatattaggggacggccggagggagtaagtatgtttccggacatgctatatatcactcgacatattttaaagagagatcatacgaatttctaatcttaagatctacttcacgacctccttctccagctttgacaaactctaccgagctccctagtggtcaattagctagggttgtggagtgggtggtgttccacggtttatagggatgctaattaatctactccctccgtccctaaatattttgacgtcgttgacttctaaatatgtttaaccattcgtcttaatcaaaaaatttaagtaattattaattctttttctatccttcgattcaatgttaaatatacttttatgtatacatatagttttacatatttcataaaagttttttaataagacaaacagttaaacatgtttaaaaaagtcaacggcgataaatatttagggacggatggagtatatttttgcgcaagttatgggaggaaaatatgaggacgcacatcctgaagagtacttaaaagaagataatgcatgcaaatacactgcaacctgcatccatggattaattgggatcttattttttcgcttatgcttatgcttatcaaccaaaatttaaattttcaatcttaaatttggatctaattttgatagttttttcattaaagtttatttttcagcctttgcttttagatcgctaagaatacatatataaaagttttattcacaaattatttttcgtttgtaaatatgtcgtttggcttatttcacgaataagcgaaacaatgacgctcttggattcttctccttaggtactactctctctattctaaaatgtggtgtactactagtatttcatctgaaaattaaccgagcaccagctgacagtacttgcacatctacattacagtatgtactagcagaagtaaatgtacttgcgcgctctgtactagcaaccaggagcaagctgatcgtactcacatgcaacaagtttgtggataaggcgtactcctacgtttttgcagttaattaacataaatagggcgagagaggaggattagtgtaattatatatgcaaagactaaagacctttcctgcaaatatttctgaggatgtcgaaggtgttatcaaaaagtgtcttttccattgcaattccagactaggcacaaattaaatttcgtaggctttgggggctttctgcgtgttatgaggaccaataatgaggtctcatcctagctaatttgctgaaatcggccgagatcttatagcagaccatatggaccgcatcatagcatgaactatgcttaggtttggctcttactttaaccgggtaaagagttgaatcgtaccaaacatgtcacgagccacatccaaatttgatttttttttcgcaaacgtgcaaaaaaattgcacgtcaatatattagaagaagagagcatagtttaattacacaacacacacggtcagaaggccgttgccaggggtcaaagaaaaaaagaaaaaaaacagataagctactaagaaagtaaaaactcatatcgcaagagagggtggggtagggggtcctactacactctggcctgcgccagaggcagcgagtcggagaagagagaagagaaggtcattgcgtgatctggagggaggggttgagagaagaggccgttgtaagccgcaacagcctcagttgacacaggacccttctcagacaccattccaagcttgcgcttcagattgtgctaggcgcgtgaggtcatgtcactcgggcaggcgagcgcgagcttggccgcaatcctcttgctacttttcggcacttcttccacggtggcatgcaattcgtggatccggtggcactaatacagttttgagatttcgtggatgatttcatctaaatgaagtccaggatctggtggcgattctatctatattgaagtccatgacatttatactctctttgtttcagactataaattcattttgactttagtcaaagtcaaactactttaagtttagttaagtttataaagagatatagtactcatatttacagcaccaaattagtttcattaaatcaataattgaatatatttatcttgggctaaaatgttactattttttcattaaacttaattaaacttaaaacaatttaactttgacaaaaaccacaatgacttatagtctgaaatggaggaagtatcgtttaatttgagactttgaatacttaatttacaaagaagtgtgtatttgtgccgtgttttctcgctctatatatatcgcagtttcacccatctaattagttgaacagtgcgtcttaatctcaatctccattcgccaattgactgtacatgtctgaatggaatccttctatgtattatctgcacagctagaatcctacaactagcgtagtgtggtggtggtcgtcttcgagttcgacgcgccgccgccgccgctcatcgcgacgacaagagcctcgccagcgtccgcagtagcagcttcctagtcttgttgcactccgagatcgtctccagcctcggcgaccacggctggttcctccgccccatcagcagcgcgtaatgccgcttcagctccggcgtgctgcacagctccgtcgccgtcgccgtcgccgtcgtcgacggcttccggcggcgcgcaccgccgccgttctcgtcgcagctgatcaccttctcgatgaactccgggaggactcggatgagcgtggcgccgccctcgcccttgacgtactcgaacgggcactgccccggcccagccacggacacctttttcgacggcgccggcgacagcgacgcgagcgccgcggcggtgagcaccttgaagcaggagaagcgctcggcggggaggacgaagtacgtccgccccgccatcagctcctcgccggcggacaccaccgggatcgggaggccgacgtagaaggagtcggccgcgcagacgacgtggtcaccggggagctccgccgtgacgtcccccgccacggcgcacaccccgccatcgtcgtccagcatcctcgtctgcccgccccagtacaccagcttcacccccgccgccgccgtcgccggcaggtgcatgcacggggagaggccgttgcccatggcaactgatgtgtctagaaccttctagaacgagctcgagcttgcagatggaagaagaggagaagaggagatgcttgtgtgaattggggctcgtggttgcgagaggggaatatatataagcgagcgttgaacggggttgactttggaaaattcaaagatggaggaggtaaatactcctactactactggtctggcttggtcagcggtcaaaggcgatggcttttgagacggaagcgggtcacgtggcagctgccgattgggccgcggagggggagaagcgagttgcgcgtgcgcatcgtcgttggtcggtgtcgccgatcgggttggatccaaccagatattcagaatttcagatcagatgcacagtagtacagttgcaagttgcaactagaagttgccgattaatttgacagcgcctggctgcaggctgctaccgcactccaacgccgacatggcaggtgtttttttttaaaaatcttgttccaaatttggagctgactgaagagagttttatactccgtattatgggtatgagttcatttatactcctatgtcacttatcatcgatttgttttcggacttattcactttgtagctattttcaaccattacaaaatttaacaattcccataattttagcaaatt</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051959.1 RefSeq:Os01g0952800]|
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| − | }}
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| − | [[Category:Genes]]
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| − | [[Category:Japonica mRNA]]
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| − | [[Category:Oryza Sativa Japonica Group]]
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| − | [[Category:Japonica Genes]]
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| − | [[Category:Japonica Chromosome 1]]
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| − | [[Category:Chromosome 1]]
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Please input one-sentence summary here.
Annotated Information
Function
OsIRO2, an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcription factor, is responsible for regulation of the genes involved in Fe homeostasis in rice. This gene has the activity of transcription, and is an important regulatory factor of grasses in the iron reaction, to be responsible for regulation of the key genes involved in Fe absorption. Under iron deficiency conditions, OsIRO2 is an indispensable factor for effective absorption to iron. In plants of OsIRO2 RNAi, due to the expression of OsIRO2 declining, the synthesis of mugineic acid is decreased. Meanwhile, the secretion of 2'-deoxy mugineic acid is reduced, resulting in a decrease of iron absorption, so that the iron content in the plants drops, leaves yellowing and its growth is inhibited. Under conditions of iron deficiency, unknown transcription factor binds into the promoter of iron deficiency OsIRO2 response cis-acting elements (IDE), to adjust the expression of OsIRO2, then OsIRO2 binds to the related gene or promoter sequences which is CACGTGG about iron absorption, regulating the expression of these genes, and promoting the absorption of iron in rice under iron deficiency conditions.
Expression
Spatial pattern of OsIRO2 expression. During germination, OsIRO2 expression was detected in embryos(Fig.1). OsIRO2 expression in vegetative tissues was restricted almost exclusively to vascular bundles of roots(Fig.2) and leaves(Fig.3), and to the root exodermis under Fe-sufficient conditions, and expanded to all tissues of roots and leaves in responseto Fe deficiency. OsIRO2 expression was also detected inflowers and developing seeds(Fig.4). Plants overexpressing OsIRO2 grew better, and OsIRO2-repressed plants showed poor growth compared to non-transformant rice after germination.[1]
