Os12g0610200

OsPIL11, with accession number Os12g0610200, is one of six putative phytochrome-interacting factors(PIFs).

Annotated Information

Constitution

OsPIL11 contain a conserved sequence motif at their N-terminal regions, designated as the active phytochrome-binding (APB) motif (active phytochrome-binding protein, also named as PIL motif). Four invariant amino acid residues(ELxxxxGQ) are critical determinants of the APB motif. This motif is necessary for binding to the biologically active Pfr form of phyB .

Function

OsPIL11 is a member of the rice phytochrome-interacting factors (PIFs) family. Nakamura et al identified six candidate genes encoding PIFs, designated OsPIL11 to OsPIL16, via homologous analysis in rice genome. OsPIL11 contain APB motif at their N-termini, suggesting the possible interaction between PIF and phytochromes in rice .

PIFs, as a small subset of the basic helix-loop-helix (bHLH) transcription factor superfamily, have been found to bind to the G-box motif in the promoter region of light-regulated genes. Thus, PIFs constitute a signal transfer pathway from photoactivated phytochromes to the light-regulation of gene expression that controls photomorphogenesis in plants. Among phytochrome associated proteins, phytochrome-interacting factors (PIFs) are central player in phytochrome-mediated signal transduction . In addition, OsPIL11 plays important roles in light signal transduction in rice leaves and its development, and it may also involve in the regulation of plant hormones.

Expression

OsPIF11 gene is widely expressed in rice roots, stems, new leaves and old leaves. The OsPIFs11 transcription factor expression level in the leaves is significantly higher than in the roots and stems, and the expression is the lowest in roots.

The expression of OsPIL11 also is organ-specific and is regulated by leaf development, abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA). OsPIL11 is involved in red light-induced de-etiolation, but not in far-red lignt-induced de-etiolation in transgenic tobacco. OsPIL11 expressed higher in the new leaves than in the old leaves. Therefore, the expression of OsPIL11 gene was regulated by hormones in rice .

Labs working on this gene

·High-Tech Research Center, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China

·College of Life Science, Henan Normal University, Xinxiang 453007, China

·College of Life Sciences, Shandong Normal University, Jinan 250014, China

·Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan

·National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China