|
|
| Line 1: |
Line 1: |
| | + | |
| | Please input one-sentence summary here. | | Please input one-sentence summary here. |
| | | | |
| | ==Annotated Information== | | ==Annotated Information== |
| | ===Function=== | | ===Function=== |
| − | Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice<ref name="ref1" />. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.
| + | Please input function information here. |
| − | [[File:1.jpg]]
| + | |
| − | The rice blast resistance gene was identified by analyzing 281 recombinant inbred lines (RILs) of an F7 recombinant inbred population of the Moroberekan/CO39 cross with 127 restriction fragment length polymorphism (RFLP) markers<ref name="ref2" />.
| |
| | ===Expression=== | | ===Expression=== |
| | + | Please input expression information here. |
| | + | |
| | ===Evolution=== | | ===Evolution=== |
| | + | Please input evolution information here. |
| | + | |
| | + | You can also add sub-section(s) at will. |
| | + | |
| | ==Labs working on this gene== | | ==Labs working on this gene== |
| | + | Please input related labs here. |
| | + | |
| | ==References== | | ==References== |
| − | <references>
| + | Please input cited references here. |
| − | <ref name="ref1">Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to ''Magnaporthe oryzae'' requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes[J]. Genetics, 2009, 181(4): 1627-1638.</ref>
| |
| − | <ref name="ref2">Wang G L, Mackill D J, Bonman J M, et al. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar[J]. Genetics, 1994, 136(4): 1421-1434.</ref>
| |
| − | </references>
| |
| | | | |
| | ==Structured Information== | | ==Structured Information== |
| − | | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 09]] |
| − | [[Category:Genes]] | |
| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]] | |
| − | [[Category:Japonica Chromosome 9]]
| |
| − | [[Category:Chromosome 9]]
| |
Please input one-sentence summary here.
Annotated Information
Function
Please input function information here.
Expression
Please input expression information here.
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this gene
Please input related labs here.
References
Please input cited references here.
Structured Information
