Os06g0102100

The rice Os06g0102100 was reported as CYP93G2 in 2010 by researchers from China.

Annotated Information

Figure 5. Analysis of the rice CYP93G2 T-DNA mutant.

Gene Symbol

• Os06g0102100 <=> OsF2H,F2H,CYP93G2

Function

• Rice CYP93G2 Is a Close Homolog of Sorghum CYP93G3
• CYP93G2 is related to the CYP93B subfamily, which consists of dicot flavone synthase II enzymes.
• CYP93G2 generates 2-hydroxy-flavanones, which are modified by O-glycosylation in transgenic Arabidopsis (Arabidopsis thaliana).
• CYP93G2 is the first enzyme that channels flavanones to C-glycosylflavone biosynthesis in rice.

Expression

• The mutant plants did not show observable differences from the wild-type plants with regard to growth, flowering time, or seed yield.

Both tricin and isovitexin are frequently O-glycosylated (Cavaliere et al., 2005), and the aglycones can be released by acid treatment.

• In the acid-hydrolyzed methanolic extracts of CYP93G2 mutant leaves, the levels of tricin detected were comparable to those of wild-type samples (Fig. 5C). However, the accumulation of isovitexin was substantially reduced in the mutant plants, suggesting that CYP93G2 is required for C-glycosylflavone production in rice. In nonhydrolyzed leaf extracts, a range of potential flavone metabolites were detected in the wild-type samples following HPLC separation, which was monitored at 350 nm (Fig. 5D).
• On the other hand, the majority of these UV light-absorbing compounds eluted at 15 to 22 min were markedly reduced in absorbance in the mutant chromatogram.

Evolution

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Labs working on this gene

• School of Biological Sciences (Y.D., H.C., C.L.) and Department of Chemistry (I.K.C.), The University of Hong Kong, Hong Kong, China