Difference between revisions of "Os09g0106700"

Revision as of 12:53, 8 May 2017

The rice Os09g0106700 was reported as PutAKT1 in 2009 ^[1] by researchers from China.

PutAKT1 is involved in mediating K+ uptake (i) both in low- and in high-afﬁnity K+ uptake range, and (ii)in P. tenuiﬂora , even under saltstress condition.The notion that AKT1-type channels are the main K+ uptake pathway into the plant root was based on their dominant expression in the roots and studies of AKT1-disrupted mutants.
PutAKT1 not only increases tissue K+ accumulation, but also decreases Na+^[2].

The AKT1 is preferentially expressed in peripheral root cell layers and root hairs.
Expression of PutAKT1 increased the K+ content under normal K+ -starvation,and NaCl-stress conditions.
The expression of PutAKT1 was induced by K+-starvation stress in the roots and was not downregulated by the presence of excess Na+.Over-expressing PutAKT1 showed enhanced salt tolerance compared to wild-type plants as shown by their shoot phenotype and dry weight.
Expression of PutAKT1 also showed a decrease in Na+ accumulation both in the shoot and in the root.

Subjected to NaCl and K+-starvation stresses for 24 h, PutAKT1 was predominantly expressed in the roots under all conditions tested (about two-fold higher than in the shoots). To gain further insight into ionic stress regulation of PutAKT1 expression in roots, PutAKT1 expression was monitored over a 24-h period.
In rice, the expression level of PutAKT1 is down-regulated by excessive external NaCl. Under K+-starvation stress, PutAKT1 was dramatically induced at 24 h of stress.

PutAKT1 belongs to the AKT1-subfamily in the Shaker K+ channel family. PutAKT1 was localized in the plasma membrane and it was preferentially expressed in the roots.there are three AKT1-type K+ channel genes in Arabidopsis and two in rice.Genomic Southern-hybridizations using DIGlabeled PutAKT1 were performed with probes from the coding region of the PutAKT1. The result revealed a number of PutAKT1 hybridizing bands, suggesting that PutAKT1 belongs to a small gene family.

Asian Natural Environmental Science Center (ANESC),The University of Tokyo, 1-1-1 Midori-Cho, Nishitokyo,Tokyo 188-0002, Japan
Department of Agronomy and Horticulture,Bogor Agricultural University (IPB), Jl. Meranti, Kampus IPB, Darmaga Bogor 16680, Indonesia S. Liu
Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin 150040, China

↑ Ardie SW, Liu S, Takano T. Expression of the AKT1-type K(+) channel gene from Puccinellia tenuiflora, PutAKT1, enhances salt tolerance in Arabidopsis. Plant Cell Rep. 2010 Aug;29(8):865-74. doi: 10.1007/s00299-010-0872-2. PubMed PMID: 20532513.
↑ Cite error: Invalid <ref> tag; no text was provided for refs named ref_1

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 * Expression of ''PutAKT1'' also showed a decrease in Na+ accumulation both in the shoot and in the root.
 ====Expression in experiment====
-Subjected to NaCl and K+-starvation stresses for 24 h, ''PutAKT1'' was predominantly expressed in the roots under all conditions tested (about two-fold higher than in the shoots). To gain further insight into ionic stress regulation of PutAKT1 expression in roots, ''PutAKT1'' expression was monitored over a 24-h period.
+* Subjected to NaCl and K+-starvation stresses for 24 h, ''PutAKT1'' was predominantly expressed in the roots under all conditions tested (about two-fold higher than in the shoots). To gain further insight into ionic stress regulation of PutAKT1 expression in roots, ''PutAKT1'' expression was monitored over a 24-h period.
-*In rice, the expression
+* In rice, the expression level of ''PutAKT1'' is down-regulated by excessive external NaCl. Under K+-starvation stress, ''PutAKT1'' was dramatically induced at 24 h of stress.
-level of ''PutAKT1'' is down-regulated by excessive external NaCl. Under K+-starvation stress, ''PutAKT1'' was dramatically induced at 24 h of stress.
 ===Evolution===