Os02g0701300

The rice Os02g0701300 was reported as OsGRF4 in 2016 by researchers from China.

Annotated Information

Gene Symbol

• Os02g0701300 <=> OsGRF4,GRF4

Function

• OsGRF4 controls grain shape, panicle length and seed shattering in rice.

Phenotypic analysis

• A unique OsGRF4 mutation, which occurs at the OsmiRNA396 target site of OsGRF4, seems to be associated with high levels of OsGRF4 expression, and results in phenotypic difference.
• Further research showed that OsGRF4 regulated two cytokinin dehydrogenase precursor genes (CKX5 and CKX1) resulting in increased cytokinin levels, which might affect the panicle traits.

Expression

• We compared the expression profiles of OsGRF4 in various organs of NILs by quantitative RT-PCR analysis with total RNA. The OsGRF4 transcript levels varied drastically among the tissues.
• OsGRF4 was preferentially expressed in developing panicles, and the highest levels of expression were found in panicles of 7 cm in length.
• there was less transcript accumulation in the rice hull, root, stem and leaf sheath. In particular, the transcript was much more abundant in NIL-OsGRF4 than in NIL-Osgrf4 in the young panicles measuring 1 cm, 4 cm, 7 cm, 11 cm and 15 cm in length.
• The differences corresponded with the critical stages of panicle traits. The OsGRF4 effect on panicle traits might be attributed to differences in expression levels.

Subcellular localization

• To determine the sub-cellular localization of OsGRF4, the coding sequence of OsGRF4 was fused with yellow fluorescent protein (YFP). In contrast, Ghd7, which is a nuclear protein, was fused with cyan fluorescent protein (CFP). Both fluorescent proteins were individually driven by the constitutive 35S cauliflower mosaic virus promoter. The constructs were co-transfected into rice protoplasts of etiolated seedlings by polyethylene glycol. The result showed that OsGRF4 co-localized to the nucleus with Ghd7.

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Labs working on this gene

• State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
• Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
• State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
• State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
• State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.