Os02g0771400

Protein Tyrosine Phosphatase

Annotated Information

Function

OsPFA-DSP2 act as negative regulators of the pathogen response in transgenic plants.Ectopic overexpression of OsPFA-DSP2 in rice increased sensitivity to Magnaporthe grisea (M. grisea Z1 strain), inhibited the accumulation of hydrogen peroxide (H2O2) and suppressed the expression of pathogenesis-related (PR) genes after fungal infection.

Expression

OsPFA-DSP2 was mainly expressed in calli, seedlings, roots, and young panicles, and localized in cytoplasm and nucleus.

Evolution

AtPFA-DSP4,which is homologous to OsPFA-DSP2,overexpressing in transgenic Arabidopsis plants,also exhibited sensitivity to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), reduced accumulation of H2O2 and decreased photosynthesic capacity after infection compared with Col-0.OsPFA-DSP2 and AtPFA-DSP4 act as negative regulators of the pathogen response in transgenic plants.

Gene Ontology Classification

GO accession	Type	Name	Code	With
GO:0009987[[1]]	biological_process	cellular process	IEA	TAIR:AT1G05000
GO:0008152[[2]]	biological_process	metabolic process	IEA	TAIR:AT1G05000
GO:0016787 [[3]]	molecular_function	hydrolase activity	IEA	TAIR:AT1G05000
GO:0005575[[4]]	cellular_component	cellular_component	IEA	TAIR:AT1G05000

PFAM hits

Accession	Name	Match Start	Match End	E-value
PF03162.6 [[5]]	Y_phosphatase2	46	198	9.8e-63

BlastP Searches (UniRef 100)

Accession	%Sim	Length	Description	P-value
UniRef100_Q0DX67 [[6]]	94.12	204	Os02g0771400 protein n=2 Tax=Oryza sativa RepID=Q0DX67_ORYSJ	4.2e-98
UniRef100_Q0DDQ5 [[7]]	89.44	161	Os06g0208700 protein n=2 Tax=Oryza sativa RepID=Q0DDQ5_ORYSJ	2.2e-69
UniRef100_Q6DL00 [[8]]	89.44	161	Dual-specificity phosphatase protein n=1 Tax=Oryza sativa Re	2.2e-69
UniRef100_A3B9I2[[9]]	89.44	161	Putative uncharacterized protein n=1 Tax=Oryza sativa Japoni	3.6e-69
UniRef100_B6U4B4[[10]]	74.77	214	Tyrosine specific protein phosphatase family protein n=1 Tax	7.5e-69
UniRef100_F2D6J8[[11]]	75.23	214	Predicted protein n=1 Tax=Hordeum vulgare subsp. vulgare Rep	7.5e-69
UniRef100_C4JAD1[[12]]	75.23	214	Putative uncharacterized protein n=1 Tax=Zea mays RepID=C4JA	4.1e-68
UniRef100_B6TUQ6[[13]]	74.65	217	Tyrosine specific protein phosphatase family protein n=1 Tax	2.9e-67
UniRef100_C5XTH0[[14]]	85.29	170	Putative uncharacterized protein Sb04g034500 n=1 Tax=Sorghum	6e-67
UniRef100_B4F971[[15]]	72.22	216	Putative uncharacterized protein n=1 Tax=Zea mays RepID=B4F9	3.3e-66
UniRef100_UPI00015CAEE8[[16]]	82.72	162	PREDICTED: hypothetical protein isoform 1 n=1 Tax=Vitis vini	3.2e-61
UniRef100_B6TNL4[[17]]	77.14	175	Tyrosine specific protein phosphatase family protein n=1 Tax	2.9e-60
UniRef100_B9T828[[18]]	86.54	156	Tyrosine-protein phosphatase SIW14, putative n=1 Tax=Ricinus	2.9e-60
UniRef100_Q9ZVN4[[19]]	85.99	157	Probable tyrosine-protein phosphatase At1g05000 n=2 Tax=Arab	5.9e-60
UniRef100_Q6K461[[20]]	84.18	158	Os09g0135700 protein n=2 Tax=Oryza sativa RepID=Q6K461_ORYSJ	9.7e-60
UniRef100_B9IAK7[[21]]	85.35	157	Predicted protein n=1 Tax=Populus trichocarpa RepID=B9IAK7_P	1.2e-59
UniRef100_UPI00015CD7AE[[22]]	82.39	159	PREDICTED: hypothetical protein n=1 Tax=Vitis vinifera RepID	1.6e-59
UniRef100_B9HZH6[[23]]	84.81	158	Predicted protein n=1 Tax=Populus trichocarpa RepID=B9HZH6_P	3.3e-59
UniRef100_B4FLL8[[24]]	82.5	160	Putative uncharacterized protein n=1 Tax=Zea mays RepID=B4FL	8.7e-59
UniRef100_B9N8H3[[25]]	84.81	158	Predicted protein n=1 Tax=Populus trichocarpa RepID=B9N8H3_P	8.7e-59

Labs working on this gene

Key Laboratory of Gene Engineering of Ministry of Education State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources School of Life Sciences,Sun Yat-sen University, Guangzhou, People’s Republic of China

Structured Information

Gene Name	Os02g0771400
Description	Putative tyrosine phosphatase family protein
Version	NM_001054792.2 GI:297599967 GeneID:4330871
Length	3886 bp
Definition	Oryza sativa Japonica Group Os02g0771400, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 2
Location	Chromosome 2:33425073..33428958
Sequence Coding Region	33425223..33425396,33425500..33425543,33425630..33425687,33428497..33428590,33428703..33428947
Expression	GEO Profiles:Os02g0771400
Genome Context	<gbrowseImage1> name=NC_008395:33425073..33428958 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008395:33425073..33428958 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atgcagctggagatttcgccgagacagaggagccagcagcagaaggaggaggaaggcgagcaccagcagcgcgccggcgaggaggcggtgggcgccgtcttcagcattgagccgtgggtggacgccgcggcggtgctggtgccgccgcttaacttcgccgaggtgaacgacggcatcttccgctccgggttccccgccgccgacaacttcgcgttcctcctctccctcaagctacgctcgatcgtgtacctgtgcccggagccgtacccggaggagaacacgcggtttctggagcagaacggcatcaagcttcaccagttcggtattgacggcagcaaggagctgcttgtaaacatccctgaagaaaaaatccgagaagcactcaaagttattcttgacgtaaggaatcaaccggtgctcatccactgcaagagaggcaagcaccggactggctgcgtcgttgggtgcctgagaaagttgcagaaatggtgcctaacttcagttttcgacgagtaccagcattttgcagcggcgaaggcgagaagtactgatcagagattcatggagctgtttgacacatccagcttgatgcacctgacggcctcacagtgttaa</cdnaseq>
Protein Sequence	<aaseq>MQLEISPRQRSQQQKEEEGEHQQRAGEEAVGAVFSIEPWVDAAA VLVPPLNFAEVNDGIFRSGFPAADNFAFLLSLKLRSIVYLCPEPYPEENTRFLEQNGI KLHQFGIDGSKELLVNIPEEKIREALKVILDVRNQPVLIHCKRGKHRTGCVVGCLRKL QKWCLTSVFDEYQHFAAAKARSTDQRFMELFDTSSLMHLTASQC</aaseq>
Gene Sequence	<dnaseqindica>3563..3736#3416..3459#3272..3329#369..462#12..256#aagaggaggatatgcagctggagatttcgccgagacagaggagccagcagcagaaggaggaggaaggcgagcaccagcagcgcgccggcgaggaggcggtgggcgccgtcttcagcattgagccgtgggtggacgccgcggcggtgctggtgccgccgcttaacttcgccgaggtgaacgacggcatcttccgctccgggttccccgccgccgacaacttcgcgttcctcctctccctcaagctacgctcgatcgtgtgagtcctatacctaggatctctttctcggccacggcgaccgtggcggcgagctcgtcgattacgtgttctgacggcggcggcgacgccttggcgtggttgatcggtgcaggtacctgtgcccggagccgtacccggaggagaacacgcggtttctggagcagaacggcatcaagcttcaccagttcggtattgacggcagcaaggtaaatgaacaagtcaaaaatcatatgcgttgagtcctttgattttctgctttattttccacggatggcaaagtcgatcttcgcatcgaattgttttttggtttttcggtcgatttggtgcgttctgcttgtggagataaataatttgcacacataatttgcaccgagaaatagcaactggtatcgcattacagtggatgcgagggttactttaggtagaacgcgctgtttacctgtttgacatgagcacgcacttgcgttttctgcgcgttgcctcgtgtcgtatggccatgagctcgttcgactactttccgttaaaaaaacatgggaactgacaactgatattgctgcttgcttcggcaaaacacttgggggcaacgcaactagggcaaacaacaactcaactagaacaaacaaaagtccctttcttgctttatctttgcccctttcagttagggcatttgcttgagtctttcaggctgaaaatgtttatcgtttttgggttttgttaacctaaattgacaaggttacgtggtgcatggtgactataaaacaccctgtttgagcaaaatgcatctagtgcgctactatttttatgttcttatttcactaattcgttttcccttctcacaaaatagtgtagaaaatctcattatggtaggagttattttttttacaatgcaaaacaacagtgtcagttaagcgggttcttgcgtcagcccagcacccatagaagattagaagttgtttacaatgctgcaaaagcgtatcttgctactggcctactgccattagcaacagtaaagcttatctttgttatgattattctggtagcgttgtgctacttttctagaagctagttcaaactgattggtagactcatggctaaggaaaatattttctatttcagcatcacgcatttcagggttaggagatttttgtctgcattatgccagtctggttctgagttttactgaatactgcaagctttaagggtaaaaaaaaaaaaagaaaacaccttaagcttaagtgatcagtgatgcagtacagcacaagttctactccctctatcaaaaaaaaaactaatcctaaatttgaatctagaaagacggagggagtagctgagaaatgatgggcttgtggagtctcctctgtgaattgggtgcctaaacaaagatcaagttgacttatgcccattattttttttaagagaacttatgtgaaaaaaaaagaggatcaagttgacttatagtataatcgcacctgtcttttaactgtcgagtgacgtctgaccagcgtaattctttcataaatttgttggccatgactttaattccgttattaaccagccacccaagattaccatttctctgtgtgccatgccatatttattctcattttactagtccttcactgaaagccattagtacggttttgtcaagctgtccatattaaaagttcatttaggcatagacgcatagtagccttggagtatgtcggtatattactccatctcgcaactttagtactcgtgtcatcgcgtggtagcatccaccgcaagtctgcaacaatgagaacatattccactgtcagttctgggttcatgaaggcatgaactgccacggcaaatggtcaaagttggtcagcgtcggcgacttaaggctgaggtcgttgcttgggttaggtattcatccctagttcatgcaaaatgacataactcattaacgtatgattaattaagttttagtttttttaaaaaaataaaattaatatgaatataaaatttaaattaactttcatatagatttcatatagatttttgttaaaaaaaacaccgttcagtaattaaaaaaatgtgcatgtgaaaatcgagtgagttaagttgggaccttcaaacaaaaaaaaactttcatcgttcattggcgattgttgctgtcgatgataaacaacacagatgtgctgaccaactttagcattcccagcaaaaaagaaatatatcgctgagtgaaaagacactgatcaggtggctgaccttactacaagtgtgtgaatggaaattagtgctcactttgacattgtctggagatgatgcttctttttcgccggactcaagctaggtgtctgaaaccactgaattacgagggtcaccggtaataattgtgtcactgctagatggattctaggatggtcactgctagatggattgtacggtggtgtttggatcaaggactaaactttagctcctgtcatatcggatgaatttagagtattaaacatagactacttataaaactaattacataaatgagagctaattcgcgtgacaaaatttttaagcctaattaatccataattagcgcatgtttactgtagcatcacataggctaattatgaattaattaggtttaatagattcgtctcgcgaattagtccggggtcatataataggttttattaatagtctatgtttaatatttataattagtgtccaaacatacgatgtgatatggactaaaaagttttagtcccatctaaacagggtctaagcttgtttctttgttaactagaaccaataatgtagtacatacttgagtggcatgacgatatctttgcatctgaactttcgataaaggtgaaccttatcagttaccgcattagcctgaaactgtgaaacaaattaaaacatccttctattgctataccatatcatcagtaattcagtatgcatccaactgaccattctcttcaatcctgttgactcttaaagttgatgcatagcctgacaataatctgaaccttgctgttacaggagctgcttgtaaacatccctgaagaaaaaatccgagaagcactcaaagttattcttggtatctgcctctacaaagatatatccaatgtctcaaaatttcatcacactatctgtatctaactcttatcgttttgatcaattcagacgtaaggaatcaaccggtgctcatccactgcaagagaggcaaggtagctatatctatttagcattgcacatccctgatatcccattttcttgaaaccttttgctacatgggaattcgacggtttcctgatattccacatgtttcagcaccggactggctgcgtcgttgggtgcctgagaaagttgcagaaatggtgcctaacttcagttttcgacgagtaccagcattttgcagcggcgaaggcgagaagtactgatcagagattcatggagctgtttgacacatccagcttgatgcacctgacggcctcacagtgttaaccgaaccgaatgttttttcatatccggtcccgatctgtaaccatgctgtctacctagcaacatattgtaatgttgctattgaaacaattcatcagagaataatgttagaaccatcattatcctcataaaagtatccatcaaaatacaagg</dnaseqindica>
External Link(s)	NCBI Gene:Os02g0771400, RefSeq:Os02g0771400