Os02g0771400

Protein Tyrosine Phosphatase

Annotated Information

Function

OsPFA-DSP2 act as negative regulators of the pathogen response in transgenic plants.Ectopic overexpression of OsPFA-DSP2 in rice increased sensitivity to Magnaporthe grisea (M. grisea Z1 strain), inhibited the accumulation of hydrogen peroxide (H2O2) and suppressed the expression of pathogenesis-related (PR) genes after fungal infection.

Expression

OsPFA-DSP2 was mainly expressed in calli, seedlings, roots, and young panicles, and localized in cytoplasm and nucleus.

Evolution

AtPFA-DSP4,which is homologous to OsPFA-DSP2,overexpressing in transgenic Arabidopsis plants,also exhibited sensitivity to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), reduced accumulation of H2O2 and decreased photosynthesic capacity after infection compared with Col-0.OsPFA-DSP2 and AtPFA-DSP4 act as negative regulators of the pathogen response in transgenic plants.

Gene Ontology Classification

GO accession	Type	Name	Code	With
GO:0009987[[1]]	biological_process	cellular process	IEA	TAIR:AT1G05000
GO:0008152[[2]]	biological_process	metabolic process	IEA	TAIR:AT1G05000
GO:0016787 [[3]]	molecular_function	hydrolase activity	IEA	TAIR:AT1G05000
GO:0005575[[4]]	cellular_component	cellular_component	IEA	TAIR:AT1G05000

PFAM hits

Accession	Name	Match Start	Match End	E-value
PF03162.6 [[5]]	Y_phosphatase2	46	198	9.8e-63

BlastP Searches (UniRef 100)

Accession	%Sim	Length	Description	P-value
UniRef100_Q0DX67 [[6]]	94.12	204	Os02g0771400 protein n=2 Tax=Oryza sativa RepID=Q0DX67_ORYSJ	4.2e-98
UniRef100_Q0DDQ5 [[7]]	89.44	161	Os06g0208700 protein n=2 Tax=Oryza sativa RepID=Q0DDQ5_ORYSJ	2.2e-69
UniRef100_Q6DL00 [[8]]	89.44	161	Dual-specificity phosphatase protein n=1 Tax=Oryza sativa Re	2.2e-69
UniRef100_A3B9I2[[9]]	89.44	161	Putative uncharacterized protein n=1 Tax=Oryza sativa Japoni	3.6e-69
UniRef100_B6U4B4[[10]]	74.77	214	Tyrosine specific protein phosphatase family protein n=1 Tax	7.5e-69
UniRef100_F2D6J8[[11]]	75.23	214	Predicted protein n=1 Tax=Hordeum vulgare subsp. vulgare Rep	7.5e-69
UniRef100_C4JAD1[[12]]	75.23	214	Putative uncharacterized protein n=1 Tax=Zea mays RepID=C4JA	4.1e-68
UniRef100_B6TUQ6[[13]]	74.65	217	Tyrosine specific protein phosphatase family protein n=1 Tax	2.9e-67
UniRef100_C5XTH0[[14]]	85.29	170	Putative uncharacterized protein Sb04g034500 n=1 Tax=Sorghum	6e-67
UniRef100_B4F971[[15]]	72.22	216	Putative uncharacterized protein n=1 Tax=Zea mays RepID=B4F9	3.3e-66
UniRef100_UPI00015CAEE8[[16]]	82.72	162	PREDICTED: hypothetical protein isoform 1 n=1 Tax=Vitis vini	3.2e-61
UniRef100_B6TNL4[[17]]	77.14	175	Tyrosine specific protein phosphatase family protein n=1 Tax	2.9e-60
UniRef100_B9T828[[18]]	86.54	156	Tyrosine-protein phosphatase SIW14, putative n=1 Tax=Ricinus	2.9e-60
UniRef100_Q9ZVN4[[19]]	85.99	157	Probable tyrosine-protein phosphatase At1g05000 n=2 Tax=Arab	5.9e-60
UniRef100_Q6K461[[20]]	84.18	158	Os09g0135700 protein n=2 Tax=Oryza sativa RepID=Q6K461_ORYSJ	9.7e-60
UniRef100_B9IAK7[[21]]	85.35	157	Predicted protein n=1 Tax=Populus trichocarpa RepID=B9IAK7_P	1.2e-59
UniRef100_UPI00015CD7AE[[22]]	82.39	159	PREDICTED: hypothetical protein n=1 Tax=Vitis vinifera RepID	1.6e-59
UniRef100_B9HZH6[[23]]	84.81	158	Predicted protein n=1 Tax=Populus trichocarpa RepID=B9HZH6_P	3.3e-59
UniRef100_B4FLL8[[24]]	82.5	160	Putative uncharacterized protein n=1 Tax=Zea mays RepID=B4FL	8.7e-59
UniRef100_B9N8H3[[25]]	84.81	158	Predicted protein n=1 Tax=Populus trichocarpa RepID=B9N8H3_P	8.7e-59

Labs working on this gene

• Key Laboratory of Gene Engineering of Ministry of Education
• State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources
• School of Life Sciences,Sun Yat-sen University, Guangzhou, People’s Republic of China

Structured Information

Gene Name	Os02g0771400
Description	Putative tyrosine phosphatase family protein
Version	NM_001054792.2 GI:297599967 GeneID:4330871
Length	3886 bp
Definition	Oryza sativa Japonica Group Os02g0771400, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 2
Location	Chromosome 2:33425073..33428958
Sequence Coding Region	33425223..33425396,33425500..33425543,33425630..33425687,33428497..33428590,33428703..33428947
Expression	GEO Profiles:Os02g0771400
Genome Context	<gbrowseImage1> name=NC_008395:33425073..33428958 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008395:33425073..33428958 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atgcagctggagatttcgccgagacagaggagccagcagcagaaggaggaggaaggcgagcaccagcagcgcgccggcgaggaggcggtgggcgccgtcttcagcattgagccgtgggtggacgccgcggcggtgctggtgccgccgcttaacttcgccgaggtgaacgacggcatcttccgctccgggttccccgccgccgacaacttcgcgttcctcctctccctcaagctacgctcgatcgtgtacctgtgcccggagccgtacccggaggagaacacgcggtttctggagcagaacggcatcaagcttcaccagttcggtattgacggcagcaaggagctgcttgtaaacatccctgaagaaaaaatccgagaagcactcaaagttattcttgacgtaaggaatcaaccggtgctcatccactgcaagagaggcaagcaccggactggctgcgtcgttgggtgcctgagaaagttgcagaaatggtgcctaacttcagttttcgacgagtaccagcattttgcagcggcgaaggcgagaagtactgatcagagattcatggagctgtttgacacatccagcttgatgcacctgacggcctcacagtgttaa</cdnaseq>
Protein Sequence	<aaseq>MQLEISPRQRSQQQKEEEGEHQQRAGEEAVGAVFSIEPWVDAAA VLVPPLNFAEVNDGIFRSGFPAADNFAFLLSLKLRSIVYLCPEPYPEENTRFLEQNGI KLHQFGIDGSKELLVNIPEEKIREALKVILDVRNQPVLIHCKRGKHRTGCVVGCLRKL QKWCLTSVFDEYQHFAAAKARSTDQRFMELFDTSSLMHLTASQC</aaseq>
Gene Sequence	<dnaseqindica>3563..3736#3416..3459#3272..3329#369..462#12..256#aagaggaggatatgcagctggagatttcgccgagacagaggagccagcagcagaaggaggaggaaggcgagcaccagcagcgcgccggcgaggaggcggtgggcgccgtcttcagcattgagccgtgggtggacgccgcggcggtgctggtgccgccgcttaacttcgccgaggtgaacgacggcatcttccgctccgggttccccgccgccgacaacttcgcgttcctcctctccctcaagctacgctcgatcgtgtgagtcctatacctaggatctctttctcggccacggcgaccgtggcggcgagctcgtcgattacgtgttctgacggcggcggcgacgccttggcgtggttgatcggtgcaggtacctgtgcccggagccgtacccggaggagaacacgcggtttctggagcagaacggcatcaagcttcaccagttcggtattgacggcagcaaggtaaatgaacaagtcaaaaatcatatgcgttgagtcctttgattttctgctttattttccacggatggcaaagtcgatcttcgcatcgaattgttttttggtttttcggtcgatttggtgcgttctgcttgtggagataaataatttgcacacataatttgcaccgagaaatagcaactggtatcgcattacagtggatgcgagggttactttaggtagaacgcgctgtttacctgtttgacatgagcacgcacttgcgttttctgcgcgttgcctcgtgtcgtatggccatgagctcgttcgactactttccgttaaaaaaacatgggaactgacaactgatattgctgcttgcttcggcaaaacacttgggggcaacgcaactagggcaaacaacaactcaactagaacaaacaaaagtccctttcttgctttatctttgcccctttcagttagggcatttgcttgagtctttcaggctgaaaatgtttatcgtttttgggttttgttaacctaaattgacaaggttacgtggtgcatggtgactataaaacaccctgtttgagcaaaatgcatctagtgcgctactatttttatgttcttatttcactaattcgttttcccttctcacaaaatagtgtagaaaatctcattatggtaggagttattttttttacaatgcaaaacaacagtgtcagttaagcgggttcttgcgtcagcccagcacccatagaagattagaagttgtttacaatgctgcaaaagcgtatcttgctactggcctactgccattagcaacagtaaagcttatctttgttatgattattctggtagcgttgtgctacttttctagaagctagttcaaactgattggtagactcatggctaaggaaaatattttctatttcagcatcacgcatttcagggttaggagatttttgtctgcattatgccagtctggttctgagttttactgaatactgcaagctttaagggtaaaaaaaaaaaaagaaaacaccttaagcttaagtgatcagtgatgcagtacagcacaagttctactccctctatcaaaaaaaaaactaatcctaaatttgaatctagaaagacggagggagtagctgagaaatgatgggcttgtggagtctcctctgtgaattgggtgcctaaacaaagatcaagttgacttatgcccattattttttttaagagaacttatgtgaaaaaaaaagaggatcaagttgacttatagtataatcgcacctgtcttttaactgtcgagtgacgtctgaccagcgtaattctttcataaatttgttggccatgactttaattccgttattaaccagccacccaagattaccatttctctgtgtgccatgccatatttattctcattttactagtccttcactgaaagccattagtacggttttgtcaagctgtccatattaaaagttcatttaggcatagacgcatagtagccttggagtatgtcggtatattactccatctcgcaactttagtactcgtgtcatcgcgtggtagcatccaccgcaagtctgcaacaatgagaacatattccactgtcagttctgggttcatgaaggcatgaactgccacggcaaatggtcaaagttggtcagcgtcggcgacttaaggctgaggtcgttgcttgggttaggtattcatccctagttcatgcaaaatgacataactcattaacgtatgattaattaagttttagtttttttaaaaaaataaaattaatatgaatataaaatttaaattaactttcatatagatttcatatagatttttgttaaaaaaaacaccgttcagtaattaaaaaaatgtgcatgtgaaaatcgagtgagttaagttgggaccttcaaacaaaaaaaaactttcatcgttcattggcgattgttgctgtcgatgataaacaacacagatgtgctgaccaactttagcattcccagcaaaaaagaaatatatcgctgagtgaaaagacactgatcaggtggctgaccttactacaagtgtgtgaatggaaattagtgctcactttgacattgtctggagatgatgcttctttttcgccggactcaagctaggtgtctgaaaccactgaattacgagggtcaccggtaataattgtgtcactgctagatggattctaggatggtcactgctagatggattgtacggtggtgtttggatcaaggactaaactttagctcctgtcatatcggatgaatttagagtattaaacatagactacttataaaactaattacataaatgagagctaattcgcgtgacaaaatttttaagcctaattaatccataattagcgcatgtttactgtagcatcacataggctaattatgaattaattaggtttaatagattcgtctcgcgaattagtccggggtcatataataggttttattaatagtctatgtttaatatttataattagtgtccaaacatacgatgtgatatggactaaaaagttttagtcccatctaaacagggtctaagcttgtttctttgttaactagaaccaataatgtagtacatacttgagtggcatgacgatatctttgcatctgaactttcgataaaggtgaaccttatcagttaccgcattagcctgaaactgtgaaacaaattaaaacatccttctattgctataccatatcatcagtaattcagtatgcatccaactgaccattctcttcaatcctgttgactcttaaagttgatgcatagcctgacaataatctgaaccttgctgttacaggagctgcttgtaaacatccctgaagaaaaaatccgagaagcactcaaagttattcttggtatctgcctctacaaagatatatccaatgtctcaaaatttcatcacactatctgtatctaactcttatcgttttgatcaattcagacgtaaggaatcaaccggtgctcatccactgcaagagaggcaaggtagctatatctatttagcattgcacatccctgatatcccattttcttgaaaccttttgctacatgggaattcgacggtttcctgatattccacatgtttcagcaccggactggctgcgtcgttgggtgcctgagaaagttgcagaaatggtgcctaacttcagttttcgacgagtaccagcattttgcagcggcgaaggcgagaagtactgatcagagattcatggagctgtttgacacatccagcttgatgcacctgacggcctcacagtgttaaccgaaccgaatgttttttcatatccggtcccgatctgtaaccatgctgtctacctagcaacatattgtaatgttgctattgaaacaattcatcagagaataatgttagaaccatcattatcctcataaaagtatccatcaaaatacaagg</dnaseqindica>
External Link(s)	NCBI Gene:Os02g0771400, RefSeq:Os02g0771400