Difference between revisions of "Os10g0419200"
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==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
| − | Rice Indeterminate 1 ('''''RID1''''') gene , which encodes a Cys-2/His-2-type zinc finger transcription factor, acts as the master switch for the transition from the vegetative to reproductive phase. Firstly, '''''RID1''''' is controlling the phase transition and initiation of floral induction. In addition, the ''Hd3a/RFL1/FTL'' complex acts as the immediate inducer of flowering. Once the phase transition is induced with the activation of '''''RID1''''', flowering signal is transduced and regulated through the various pathways and eventually integrated with FT-like proteins to induce flowering<ref name="ref1" />. | + | Rice Indeterminate 1 ('''''RID1''''') gene , which encodes a Cys-2/His-2-type zinc finger transcription factor, acts as the master switch for the transition from the vegetative to reproductive phase. Firstly, '''''RID1''''' is controlling the phase transition and initiation of floral induction. In addition, the ''Hd3a/RFL1/FTL'' complex acts as the immediate inducer of flowering. Once the phase transition is induced with the activation of '''''RID1''''', flowering signal is transduced and regulated through the various pathways and eventually integrated with FT-like proteins to induce flowering <ref name="ref1" />. |
| + | |||
| + | ===Mutation=== | ||
| + | A '''''RID1''''' knockout (''rid1''), mutated by T-DNA insertion, never headed after growing for >500 days under a range of growth conditions and is thus referred to as a never-flowering phenotype. Figure 1 shows the phenotypes of ''rid1'' mutant (Left) and WT (Right) plants at maturity stage<ref name="ref1" />. | ||
| + | This mutation-suppressed expression of the genes is known to be involved in flowering regulation, especially in the ''Ehd1/Hd3a'' pathway and a series of ''RFT'' homologs<ref name="ref1" />. | ||
===Expression=== | ===Expression=== | ||
| − | + | The RT-PCR analysis revealed a low but detectable level of '''''RID1''''' transcript in immature leaves, but none in the root, mature leaves, SAM, or other tissues examined. When expressing the '''''RID1''''' fused in frame with a GFP, signal was clearly detected in the outer epidermal cell of immature leaves and in the region immediately beneath the meristem where internodes are visible but not in other tissues<ref name="ref1" />. | |
| − | === | + | ===Subcellular localization=== |
| − | + | The subcellular localization analysis by ''Changyin Wu et al''. suggests that '''''RID1''''' is a nuclear protein, which is expressed in nucleus<ref name="ref1" />. | |
| − | + | ===Extention=== | |
| + | ''Changyin Wu et al.'' proposed a model to place '''''RID1''''' in the molecular pathways of flowering regulation in rice (Fig.2). There are two indispensable elements in the system. The first is the '''''RID1''''' gene controlling the phase transition and initiation of floral induction. The other is the ''Hd3a/RFL1/FTL'' complex acting as the immediate inducer of flowering. Completely abolishing the function of either element would cause never-flowering. Once the phase transition is induced with the activation of '''''RID1''''', flowering signaling is transduced and regulated through the various pathways, including ones yet to be identified. Signals from these pathways are eventually integrated with proteins of FTL genes such as Hd3a and RFT1 and thus induce flowering<ref name="ref1" />. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
Revision as of 06:14, 16 May 2014
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Contents
Annotated Information
Function
Rice Indeterminate 1 (RID1) gene , which encodes a Cys-2/His-2-type zinc finger transcription factor, acts as the master switch for the transition from the vegetative to reproductive phase. Firstly, RID1 is controlling the phase transition and initiation of floral induction. In addition, the Hd3a/RFL1/FTL complex acts as the immediate inducer of flowering. Once the phase transition is induced with the activation of RID1, flowering signal is transduced and regulated through the various pathways and eventually integrated with FT-like proteins to induce flowering [1].
Mutation
A RID1 knockout (rid1), mutated by T-DNA insertion, never headed after growing for >500 days under a range of growth conditions and is thus referred to as a never-flowering phenotype. Figure 1 shows the phenotypes of rid1 mutant (Left) and WT (Right) plants at maturity stage[1]. This mutation-suppressed expression of the genes is known to be involved in flowering regulation, especially in the Ehd1/Hd3a pathway and a series of RFT homologs[1].
Expression
The RT-PCR analysis revealed a low but detectable level of RID1 transcript in immature leaves, but none in the root, mature leaves, SAM, or other tissues examined. When expressing the RID1 fused in frame with a GFP, signal was clearly detected in the outer epidermal cell of immature leaves and in the region immediately beneath the meristem where internodes are visible but not in other tissues[1].
Subcellular localization
The subcellular localization analysis by Changyin Wu et al. suggests that RID1 is a nuclear protein, which is expressed in nucleus[1].
Extention
Changyin Wu et al. proposed a model to place RID1 in the molecular pathways of flowering regulation in rice (Fig.2). There are two indispensable elements in the system. The first is the RID1 gene controlling the phase transition and initiation of floral induction. The other is the Hd3a/RFL1/FTL complex acting as the immediate inducer of flowering. Completely abolishing the function of either element would cause never-flowering. Once the phase transition is induced with the activation of RID1, flowering signaling is transduced and regulated through the various pathways, including ones yet to be identified. Signals from these pathways are eventually integrated with proteins of FTL genes such as Hd3a and RFT1 and thus induce flowering[1].
Labs working on this gene
- National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China
- Department of Crop Genetics, John Innes Centre, Norwich NR4 7UH, United Kingdom
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Changyin Wu, Changjun You, Caishun Li, Tuan Long, Guoxing Chen, Mary E. Byrne, and Qifa Zhang (2008). RID1, encoding a Cys2/His2-type zinc finger transcription factor, acts as a master switch from vegetative to floral development in rice. Proc. Natl. Acad. Sci. USA 105,12915 -12920.
Structured Information
| Gene Name |
Os10g0419200 |
|---|---|
| Description |
Zinc finger, C2H2-type domain containing protein |
| Version |
NM_001071151.1 GI:115482045 GeneID:4348644 |
| Length |
2849 bp |
| Definition |
Oryza sativa Japonica Group Os10g0419200, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 10:15197103..15199951 |
| Sequence Coding Region |
15197103..15197850,15199375..15199798,15199894..15199951 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008403:15197103..15199951 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008403:15197103..15199951 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggcggcggcgcaggagccgagagccaagaagaagaggagcctccccggcaacccagacccggaggcggaggtgatcgcgctgtcgccgcgggcgctggtggcgacgaaccgcttcgtctgcgaggtctgcaacaaggggttccagcgcgaccagaacctgcagctccaccgccgcggccacaacctcccctggaagctccgccaccgcgccgccgctgtctccgccgtgacgacagcggcgccggcgccgaggaagagggtgtacgtgtgcccggagccgacgtgcgtgcaccacgacccggctcgggcgctgggcgacctgaccgggatcaagaagcacttctcgcggaagcacggggagaagcggtggcggtgcgagcggtgcgggaagaggtacgccgtgcactccgactggaaggcgcacgtcaagaactgcggcacccgcgagtaccgctgcgactgcggcatcctcttctccaggaaggacagcctgctgacacacagggccttctgcgatgccctagctgaagagagtgcaaggcttcttgctgctgcaaacaacagcagcagcatcactactaccacctgcaacaacagcaacatcagcagcaacaacaacaacaacaacattaacagcattagcaatagcaataatcttctgatcaccagcagcagcagctcaccaccactgttcctgccattctccaccactcctgctgaaaaccctaaccctaatcagctcctgttcctgcaacaacaccaagcagctcaccaccagctgcttcttcctcagttccagcagccaccctcctcgccgccggcctacttcgatcacctcgcattcggcggcggcggcggcgtcatcaccggcagcagctgcaacgacgacaatagctcgatcgccggcgatgtcatggtggctgcaggtggtgacagtgtcagcttcgggcttacctcggaaggctccgtgaccatgcacgccggcgacgtcggacggcgccggctcactagggacttcctcggcgtcgatcacgacgccggcgaggtcgacgagctcgagcttgatgagctgccggctgatctgtccaccaccgccgccgcctgccagggttgcaacttcgccgccgccaccaccgccgcctgctgcgccaccgacttcaccaccggcagcaggcagtacctcggccggctgccgccggtgaacgagacgtggagccacaacttctaa</cdnaseq> |
| Protein Sequence |
<aaseq>MAAAQEPRAKKKRSLPGNPDPEAEVIALSPRALVATNRFVCEVC NKGFQRDQNLQLHRRGHNLPWKLRHRAAAVSAVTTAAPAPRKRVYVCPEPTCVHHDPA RALGDLTGIKKHFSRKHGEKRWRCERCGKRYAVHSDWKAHVKNCGTREYRCDCGILFS RKDSLLTHRAFCDALAEESARLLAAANNSSSITTTTCNNSNISSNNNNNNINSISNSN NLLITSSSSSPPLFLPFSTTPAENPNPNQLLFLQQHQAAHHQLLLPQFQQPPSSPPAY FDHLAFGGGGGVITGSSCNDDNSSIAGDVMVAAGGDSVSFGLTSEGSVTMHAGDVGRR RLTRDFLGVDHDAGEVDELELDELPADLSTTAAACQGCNFAAATTAACCATDFTTGSR QYLGRLPPVNETWSHNF</aaseq> |
| Gene Sequence |
<dnaseqindica>2102..2849#154..577#1..58#atggcggcggcgcaggagccgagagccaagaagaagaggagcctccccggcaacccaggtgagcgagaaactgcctagctcttcttcttcttcttcttgcttgatcgatctcatctcaattctgctgtctcgccatggtgttggatcgatcagacccggaggcggaggtgatcgcgctgtcgccgcgggcgctggtggcgacgaaccgcttcgtctgcgaggtctgcaacaaggggttccagcgcgaccagaacctgcagctccaccgccgcggccacaacctcccctggaagctccgccaccgcgccgccgctgtctccgccgtgacgacagcggcgccggcgccgaggaagagggtgtacgtgtgcccggagccgacgtgcgtgcaccacgacccggctcgggcgctgggcgacctgaccgggatcaagaagcacttctcgcggaagcacggggagaagcggtggcggtgcgagcggtgcgggaagaggtacgccgtgcactccgactggaaggcgcacgtcaagaactgcggcacccgcgagtaccgctgcgactgcggcatcctcttctccaggtccggccacccccacgctcgccgttaaacacacacctttgattactatttattactactccctccatgctctaatacaacggattttgacattttacttgcactatttaactattcgtcttatttaaatttttttggaattattatttattttatttgtgacttactttattattcaaaatctttaaacacaacttctcgttttttatatttgcacaaattttttttaataagacgagtggtcaaaactacaaataaaatatcaaaatctcttatattagaggagagcgagtagctcttaattaaacttctttgcatatgctattgcattaaaaaagaacatctctgaaaaaaaaaacatggatggacactgatcaactaccaactgttaattcatcactacttcgagaggttttgagtattttggcagtataataccttgatatatatcttccaatttcgaagctagtgttcttcttcctgttcttgctcttgtttcctagctagcccaggctttctgaacttttagtccgaagttaagcatatactctcactggatctggtgttttatgtgaatttcatctgaaaattctgcatttgcaaacgggctttttgaaggtttcaatatgtgcttgttattaatctctaatgcatacatggtgcttatcatgtacgtcacacacacacacacacacacacacacacacacacacatatatatataatcatactcgaaagttttatatttcaaatatgtttttgaaaacctgcaaatcctgcatgcagctagctagctagtccggggcctttttcaatggttgggcatgcatgtggccatgagatctccatcagaatttcaaagccatcttcatcatcatatctgcatttgcaggaggcattgatggaaacacttcatttatagataatttgctgctgcagtaggatcgctttcttgctcccttttcttgccacagttcctgcattagcaattgaaatttaaggaggcagtttcaaggagcagatatagatatatattggcttatagttagctatagctagcagctgggggaccccaagatacacaataattatgtacactaagtttacacatgtaattaattatctatgcaacactaccaaaaatattgatctcaagaagttaattaattaaacagacactaaaaatctctaacatttaccctgcttgtagctctaactagcatgtaaatccatgaacctgaacatccaatgatcacacacgcgcacaaaccttggaaaagaaactaagctaatcatacggcaaggagacctttcttcaccaaagttaaatgcaactttacatgcatgcatgcgttcatcactgatcatgtgcacatcatcactactgttaccctacaaaatttgggaaatcaaacataaataatcactaaaaaactctttcatctgatcaatccacagtcgtccttaattaaattaacagttgacaaaaatacaataattccaattaattgttcatgcctgcaggaaggacagcctgctgacacacagggccttctgcgatgccctagctgaagagagtgcaaggcttcttgctgctgcaaacaacagcagcagcatcactactaccacctgcaacaacagcaacatcagcagcaacaacaacaacaacaacattaacagcattagcaatagcaataatcttctgatcaccagcagcagcagctcaccaccactgttcctgccattctccaccactcctgctgaaaaccctaaccctaatcagctcctgttcctgcaacaacaccaagcagctcaccaccagctgcttcttcctcagttccagcagccaccctcctcgccgccggcctacttcgatcacctcgcattcggcggcggcggcggcgtcatcaccggcagcagctgcaacgacgacaatagctcgatcgccggcgatgtcatggtggctgcaggtggtgacagtgtcagcttcgggcttacctcggaaggctccgtgaccatgcacgccggcgacgtcggacggcgccggctcactagggacttcctcggcgtcgatcacgacgccggcgaggtcgacgagctcgagcttgatgagctgccggctgatctgtccaccaccgccgccgcctgccagggttgcaacttcgccgccgccaccaccgccgcctgctgcgccaccgacttcaccaccggcagcaggcagtacctcggccggctgccgccggtgaacgagacgtggagccacaacttctaa</dnaseqindica> |
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