Difference between revisions of "Os02g0580300"
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==References== | ==References== | ||
| − | <ref name="ref1"> Sasaki A.;Itoh H.;Gomi K.;Ueguchi-Tanaka M.;Ishiyama K.;Kobayashi M. Jeong DH.;An G.;Kitano H.;Ashikari M.;Matsuoka M Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant. Science, 2003, 299: 1896-1898. | + | <ref name="ref1"> Sasaki A.;Itoh H.;Gomi K.;Ueguchi-Tanaka M.;Ishiyama K.;Kobayashi M. Jeong DH.;An G.;Kitano H.;Ashikari M.;Matsuoka M Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant. Science, 2003, 299: 1896-1898.</ref> |
| − | <ref name="ref2"> Nicholas P. Harberd. Relieving DELLA Restraint . Science, 2003, 299(5614): 1853-1854. | + | <ref name="ref2"> Nicholas P. Harberd. Relieving DELLA Restraint . Science, 2003, 299(5614): 1853-1854.</ref> |
| − | <ref name="ref3"> Kenji Gomi;Akie Sasaki;Hironori Itoh;Miyako Ueguchi-Tanaka;Motoyuki Ashikari;Hidemi Kitano;Makoto Matsuoka. GID2, an F-box subunit of the SCF E3 complex, specifically interacts with phosphorylated SLR1 protein and regulates the gibberellin-dependent degradation of SLR1 in rice. The Plant Journal, 2004, 37(4): 626-634 | + | <ref name="ref3"> Kenji Gomi;Akie Sasaki;Hironori Itoh;Miyako Ueguchi-Tanaka;Motoyuki Ashikari;Hidemi Kitano;Makoto Matsuoka. GID2, an F-box subunit of the SCF E3 complex, specifically interacts with phosphorylated SLR1 protein and regulates the gibberellin-dependent degradation of SLR1 in rice. The Plant Journal, 2004, 37(4): 626-634.</ref> |
==Structured Information== | ==Structured Information== | ||
Revision as of 08:54, 17 May 2014
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Contents
Annotated Information
Function
The GID2 gene encoded a 639-base-pair open reading frame capable of producing a polypeptide of 212 amino acid residues. The deduced amino acid sequence of GID2 contained an F-box domain[1].F-box domains are found in specific components of the multisubunit SCF E3 ubiquitin ligase complex.[2]The F-box protein in the SCFcomplex functions as a receptor that selectively recuits target proteins into the complex to degrade those proteins through ubiquitination[1]. GID2 influences the rice height through regulating GA signaling. GA are essential regulators of diverse growth and developmental process in plants. A GAsignal promotes the degradation of SLR1 by the post-translational modification to be targeted by GID2[3]. GID2 is a component of the SCF complex through an inieraction with a rice ASK1 homolog,OsSkp 15.GID2 is a positive regulator of GA signaling and that regulated degradation of SLR1 is initiated through GA-dependent phosphorylation and finalized by an SCFGID2-proteasome pathway.The SLR1 protein functions as a repressor of GA signaling in rice and its degradation is essential for downstream action of GA. The SCF-GID2 complex mediate the ubiquition/26S proteasome pathway[2][3].
Domain analysis of GID2
Comparison of the primary structure between GID2 and Arabidopsis ortholog protein, SLR1, revealed that these F-box proteins share three conserved domains, the F-box, GGF, and LSL domains. In addition to these domains, GID2 has a unique N-terminal region(VR1), which is not shared with SLY1. The dwarf phenotype of gid2 was not rescued by the introduction of contructs such as ΔF,ΔGGF,or ΔLSL; however, it was rescued by the intact GID2 and ΔVR1. This result demonstrates thar all the conversed domains pointed out earlier are essential for the function of GID2 unlike the N-terminal region of GID2, which is not shared with SLY1[3].
Expression
The GID2 gene is expressed at different levels in all organs, preferentially expressed in unopened flowers, shoot apices, and elongation stem, and at lower levels in the leaf blades, leaf sheaths, roots, and rachis. Expression of GID2 preferentially occurs in rice organs actively synthesizing GA[3].
Mutant
The gid2 is a rice GA-insensitive dwarf mutant. It is caused by the loss function of a putative F-box protein. The gid2 mutant shows a severe dwarf phenotype with wide leaf blades and dark green leaves, fetures typical of GA-related mutants such as d1 and d18. The gid2-1/slr-1 double mutants show a slender phenotype identical to that of the slr-1 single mutants, indicating that GID2 functions upstream of SLR1[1]. The gid2 mutants are dwarfed because they accumulate SLR1.The gid2 mutants exhibit reduced GA responses and don’t resume normal growth when treated with GA[2]. In gid2, a repressor for GA signaling, SLR1, is highly accumulated in a phosphorylated form and GA increases its concentration in the wild type.Dgradation of SLR1 protein does not occur in gid2 mutants. The accumulated SLR1 is located in the nuclei, as it is in the wild type[1].
Evolution
The F-box domain in GID2 is well conserved in other F-box proteins, with the greatest similarity to Arabidopsis SLY1, which is a positive regulator of GA signaling[1].F-box proteins occur widely throughout the eukaryote kingdom, in organisms ranging from yeast to humans, and they function as receptors that recuit proteins as substrates for ubiquitin-mediated degradation in the 26S proteasome[3]. The SCF-mediated signaling pathway is well conserved in yeast, mammals and higher plants. GA-dependent phosphorylation of SLR1 triggers the ubiquitin-mediated degradation in a manner similar to the SCF-mediated in yeast and animals[1].
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Labs working on this gene
1 BioScience Center, Nagoya University, Nagoya, Japan.
2 BioResources Center, Riken, Tsukuba, Japan.
3 Division of Molecular and Life Science, Pohang University of Science and Technology, Republic of Korea.
4 Graduate School of Bioagricultural Science, Nagoya University, Nagoya, Japan.
References
Structured Information
| Gene Name |
Os02g0580300 |
|---|---|
| Description |
Similar to 14-3-3 protein 6 |
| Version |
NM_001053769.1 GI:115446908 GeneID:4329775 |
| Length |
4099 bp |
| Definition |
Oryza sativa Japonica Group Os02g0580300, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 2:23191177..23195275 |
| Sequence Coding Region |
23193203..23193612,23193701..23193779,23193940..23194062,23194724..23194840,23194935..23194994 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008395:23191177..23195275 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008395:23191177..23195275 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgtcgcagcctgctgagctttcccgtgaggagaatgtgtacatggctaagcttgcagagcaggccgagaggtatgaggagatggttgagttcatggagaaggttgctaagacagttgactctgaggagctcactgttgaggagcgcaaccttctatcagttgcttacaagaatgttattggtgctcgccgtgcgtcatggcgcatcatatcatccattgaacagaaggaagagagccgtggtaatgaggatcgttgcacgctcatcaaggaatacaggggaaagattgaaactgagctctccaagatctgtgatggcatcctcaagcttcttgactcccaccttgtgccttcatccactgctccagagtccaaggtcttctacctcaagatgaaaggcgactactacaggtacctcgcagagtttaagactggagctgagaggaaggatgctgctgagaacaccatggtggcatacaaagccgctcaggatattgccctggcagagttgcccccaactcatcctatcagacttgggctggccctcaacttctcggtgttttattacgagatcctcaactctcctgaccgtgcttgcaatcttgcaaagcaggctttcgatgaggctatctcagagctggacactctgagtgaggaatcctacaaggacagcactttgatcatgcagcttctgcgtgataacctgacgctgtggacttccgatatctcggaggatgctgctgaggaaatcaaggaggcccccaagggcgaatcaggagatggacagtga</cdnaseq> |
| Protein Sequence |
<aaseq>MSQPAELSREENVYMAKLAEQAERYEEMVEFMEKVAKTVDSEEL TVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEDRCTLIKEYRGKIETELSK ICDGILKLLDSHLVPSSTAPESKVFYLKMKGDYYRYLAEFKTGAERKDAAENTMVAYK AAQDIALAELPPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAFDEAISELDTLSE ESYKDSTLIMQLLRDNLTLWTSDISEDAAEEIKEAPKGESGDGQ</aaseq> |
| Gene Sequence |
<dnaseqindica>2027..2436#2525..2603#2764..2886#3548..3664#3759..3818#acaggagaagccgcagccactgaggtaaactgctcctcctcgccgatttcccccctcctttttctctaataccaaattactcgagaaattgtgttggttcggtccgcgggtagcgtgattcggccagatctggcgtctgtgtgtgtggagaattcgagggttttgtttgggtttgtggggggagtaaatccggacgaatctgtggtggtttgggggggaattcggtgtgtgtttgagttgatttgagttcgtggatgattccgttggatattgataggtcctagttctgatccagaagtggcggctacgcctttgctgtagcctcaccgatccgccgccgccgccgccatgaagttccgctctgattcgtcaggcggcgacgagccccgcgctccggctgccggcgacggcggcggcggcggcgacgagccggccaagaggcagcggaccgatccgtcctccagttcgagccagggcgaggcctcctcttcctcgcagccgccaccgcagcagcagcaggaggagcagcctccggaggatgcgggagagggcgagcagccgagggttccggatctcggggaggacctggtgttcgaggtgctgcggcgagcggaggcgcggacgctggcggccgcggcgtgcgtgagcagggggtggcggcagctcgcggaggacgagcggctctgggaggccgcgtgcgtgcgggagtgggcgaacctcggcttctccgagcggcagctccgggccgtggtgctctccctcggtggattccgccggctccacgctgtctacatccgccccctgcagtggcgtggcgccggcgtgcccaggcaacaggggaggcggcagccgccggtgaggttgggccgggaccaggttcagctctcgctgtcactgttctccattgggttctttcagaatatgccttgtcctaagaaagacaagggaaatgacagtgataagaatggagggggccaatgcgggtagtcgaatttgtggaataagcttcactccaacaagcatcatcctaagctaccatggcagttgccctattgttcacactggtgatgctagagagtgttggagagacgaacaggaaaatcggcatgcttgatttgggacatgagagaaattaagtggaattgcaagatctgaattggttcggttgtgttccgcacttgtaagatttatgtgatagtgtcacatggattgatggacgttgagtgctatgaggacagttgcgtgaatgctgatgacaagtgatttacttcaagagaagggagaaatggtttgcaattcgttctgctgttgctgatattctagttatcagctattgtgtcaacttgatatatctggcccaagagcagtatggactgtgtgccttcttgcgccggtaccaagctttagagggaaagctgatgcaaatagcttttgtttgcataacctttttcaattagggaaacataatttcccatattaggcatatggagaattatgtgaggtattgattgtttgtacaaaaataaccattttattcttgaatattgcattttcacatgttaactaaggttcttaagttgatctgactgccatgtacaatatggagcccaacaaggggtagtgacacatatttctcaacttcagtatgcaataagtcagtcattttatatgtttttacatgtggagctgcggataatgtgacaaacttatgtaatcgtgaatgatcttttgatgtttctctgtttttctttcatgtagtttgatataggattcctttatctggcactattgaagttttgttgtaaataatgttattcttgcaaagattatcttaaatcaatcatttctggatctgaagaggtattgtaacaatctgaagctttgcacaaccaattgcaactaaacttttgtatttcttagggtgaggacttgaggttggtgtgatgggcatttagctaactgaaatttctctcaaacctcacagatttgaccttctgtttctaccagaaaaacacaaacagtgaagatgtcgcagcctgctgagctttcccgtgaggagaatgtgtacatggctaagcttgcagagcaggccgagaggtatgaggagatggttgagttcatggagaaggttgctaagacagttgactctgaggagctcactgttgaggagcgcaaccttctatcagttgcttacaagaatgttattggtgctcgccgtgcgtcatggcgcatcatatcatccattgaacagaaggaagagagccgtggtaatgaggatcgttgcacgctcatcaaggaatacaggggaaagattgaaactgagctctccaagatctgtgatggcatcctcaagcttcttgactcccaccttgtgccttcatccactgctccagagtccaaggtcttctacctcaagatgaaaggcgactactacaggtattacaatagttcctggcatagcagtctttaatttctatttctttatattgtcaagttcatgtgtttgaatgtatgtgtttgataggtacctcgcagagtttaagactggagctgagaggaaggatgctgctgagaacaccatggtggcatacaaagccgctcaggtggtttccttaacagtgaatttgttgttgcggcatcctgtttgtttgtttgtgccattttgcaattttgattcggggtattcagctaaagttatttgtgatgtgttctcctttggtctgttttgtttttctgaatattgttggtgataaaatctttcaggatattgccctggcagagttgcccccaactcatcctatcagacttgggctggccctcaacttctcggtgttttattacgagatcctcaactctcctgaccgtgcttgcaatcttgcaaagcaggtttaatttctactgcattctatttcattatttgtgaattggaggaccatatggagttgagaagatattttgaagtttataccattgttgtgaaatataatgtcttagtaattgggattgaacaaataacatgtatgaattagtaatacacctggaacaggttctacttagttctgttagcaaatagcaaagtgtaggctccttaatgcaagtaagctgaacgggttaggtgcattgtcatgatggattttcttcattaagaatacttgatagaaaatgatgaatcaaataagaggtgtaagaaattatagtttacagttacagatttgcccaccttgaatacttgagtgcttctaaataactgatgaagtccgagcatcactatggccattgtgacgtggaacaagcttgtctcaatatggccatcgttcattcatagaggcctaccagtttgttctgtaatttgttaataatagatatctgatgtatatagaatggtttactgcagacaagtttgctataagttcactgctttgtccatggatataattgatcttggacttggagcacacattatgtgccttactttatgaaacttgcttgtgctagtacttatatttgccatcataatagaatgtgacagtaacctactttatctcaggctttcgatgaggctatctcagagctggacactctgagtgaggaatcctacaaggacagcactttgatcatgcagcttctgcgtgataacctgacgctgtggacttccgatatctcggtattttcttcgccgctagcatatatatttgcacttgcatcatattgtctttggaatcatcttaaatgcgtaaattccatgtgtgcggcttcaggaggatgctgctgaggaaatcaaggaggcccccaagggcgaatcaggagatggacagtgaacatgatcgaatgcgtgcgcccacaaactagaatagtgacgctgcaaatgtgctgtgggttatcgtttcattttatagattgtaatgtctcgtgatttgtcggaaacaggaggcgctccccatttatgttgtgctaggaaaccttgcagctgactcgctgcttggctggtggattctcgagctgcactttctttggggggagcctcagggtggaaccttcatgtcacttattggatcgattgttttcggtatgtcagttgtgagccttatctccagagtcc</dnaseqindica> |
| External Link(s) |
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Sasaki A.;Itoh H.;Gomi K.;Ueguchi-Tanaka M.;Ishiyama K.;Kobayashi M. Jeong DH.;An G.;Kitano H.;Ashikari M.;Matsuoka M Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant. Science, 2003, 299: 1896-1898.
- ↑ 2.0 2.1 2.2 2.3 Nicholas P. Harberd. Relieving DELLA Restraint . Science, 2003, 299(5614): 1853-1854.
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 Kenji Gomi;Akie Sasaki;Hironori Itoh;Miyako Ueguchi-Tanaka;Motoyuki Ashikari;Hidemi Kitano;Makoto Matsuoka. GID2, an F-box subunit of the SCF E3 complex, specifically interacts with phosphorylated SLR1 protein and regulates the gibberellin-dependent degradation of SLR1 in rice. The Plant Journal, 2004, 37(4): 626-634.
