Os05g0586200

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==Annotated Information

Function

Figure1:Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference).

Figure2:The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference).

OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice by the use of an osjar1 Tos17 mutant.It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that OsJar1participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.OsJar1 modulates light and JA signalling in the photomorphogenesis of rice. Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. OsJAR1 is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS.

Expression

(1)OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA).The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile.

(2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.

For RT-PCR, first-strand cDNA synthesis kit for RT-PCR (Roche, Mannheim, Germany) was performed according to the manufacturer's description. A 1 µL aliquot of a 1:10 dilution of the RT product was used as a template in the subsequent PCR reaction. For amplification of OsJAR1 cDNA, the primers JAR1F1 and JAR1R2 were used, for ubiquitin Rubg237F and Rubg 304R. The PCR conditions were 1 min 94 °C, 30× (94 °C, 1 min; 60 °C, 1 min; 72 °C, 2 min), 72 °C, 7 min.

Primers used in real-time PCR and RT-PCR:

JAR1F1: TAAGGCATTACGCAGGGAAC

JAR1F2: AGTGCTGAGCATCAACATCG

JAR1R1: CCAGATAACAGCACCAGAAGC

JAR1R2: CACCACCAAGGCTTAGGAAA

Rubg237F: GAGCCTCTGTTCGTCAAGTA

Rubg304R: ACTCGATGGTCCATTAAACC

Evolution

OsJar1 belongs to the GH3 family in rice. Another member of this family in Arabidopsis, DWARF IN LIGHT 2 (DFL2), showed 47% identities. Dfl2 was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003). OsJAR1 contained three domains described to be important for the enzymatic function of GH3 proteins (Chang, Xiang & Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).

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Labs working on this gene

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References

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Structured Information

Gene Name	Os05g0586200
Description	GH3 auxin-responsive promoter family protein
Version	NM_001062995.1 GI:115465720 GeneID:4339756
Length	5516 bp
Definition	Oryza sativa Japonica Group Os05g0586200, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 5
Location	Chromosome 5:29281780..29287295
Sequence Coding Region	29284407..29284687,29284793..29284894,29285094..29285911,29286499..29287043
Expression	GEO Profiles:Os05g0586200
Genome Context	<gbrowseImage1> name=NC_008398:29281780..29287295 source=RiceChromosome05 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008398:29281780..29287295 source=RiceChromosome05 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccgtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttga</cdnaseq>
Protein Sequence	<aaseq>MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF GLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKP KFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATT NLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAH SLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIG LSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNI DPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIY EVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVE EASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDA GYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQIL SRNVTQSYFSTAYGF</aaseq>
Gene Sequence	<dnaseqindica>2628..2908#3014..3115#3315..4132#4720..5264#acccgctctctcctctcatctcctcctcctgtgttcgtgttcttcttcttcttgtagcagcgcttaaccaacccatcattagcgaccaaacctctctccctctccccctctgactaatcaaaattaaacaatttcgatctccaccttcctcttcttgattccagttccaccagagagagacagggtcttctctaatccatgtggtgcttgttggtctcgcttgcttcgtcttcgtcttcgtcttcctcttctcgcacctagtcccgcctcgtcgcctcctccggagaccggagccggacgggacggttgccggcgagcggcggtcgctttcttctctcggccctactcacggtcagtgattggctcccttccttcctggtgttctattgatcctttgtgggggaaaaaaggcttcgatttggggggcatgttcttgcctttttcctgttttgggggagacgagacggatttcaatatatttttttaatctggggggattatgtgtggaaaagattgttcctcgtttatctgggtagtatttggagggtcctatggggatattctttgtaaagctgggatttttaggtggtaaaataatgttgatatactttcagaaagaatatactgcagaaagtttttcagagataacagataagattaggccgtccttaatctcctttctttgcgtctgttccctccaactaggatggaaaagaatggcggattgattagtcagaaactggctgctgaatactctgagatttcatttcatttcagcctctgttgattcctcatggctgtctctaacttctttgcttgtgcttccgatttctttggcggtaaatagcctccacgaatctctggtcaacgaggtattaaaacttaaaagggcttttgtctcaggttgacccgagccatgattcgcccccctcccatgctttgcaaacaagatatgatacgcagtgctggtacaattcagagcatatgttctttcctcttttgctttgtttcagacagcaacttttagcagccacgcctctcacaagttgataaaaaaaaattttgtgttgctctcctactactcatcactttctagtgcgatatagtagtagtagtagtaggagtatatgctagcactatccacagtttagcacacagattggtggcacctgggcagatataaaagcttcacatttttatcaatgtctgcgcagttatattgtcgatttgattccttttatagatattactaggtcaagtcgtgtcgcaattactccccccctttcctccattgtagggtgttcctgtttagtccgcgggggatgctgctccctgagcctgtgtgtgtctcttgatttttctttctttgctcgcacgcagcagcagcagcatgttttgtgtcggtgttaaggaccctacataaataattcatcaaatcaagtatcttttgtctgttttggggaatctcctttgcggttcgtccaaaatttcgcgaaagaaacaattctatatgactgcttttgtcgagaggttagacatcactgctagagtagtagagcaatgctacacgtactaaatttttcttactaaacttttactaataatcatctcaaccgtttgatttaagtagatggaatttacaaaaaatctagatgcactcgtagcatgacacatcagtgttagtaagaatttagtaagaaaaagttagtacgtatagccttttccagagtagtactagcagagtgttagattgtatcacctgctcgggcagttgtaaatggtatggtgagttggtgactggtttttacagttttttcatacttgggtttatggaggatttactgttatggagacctgtaaaaaatgttacatatattgctactttctcatttggatttcggatggctgttttttttatcattatgttggtaaagttttgcatcaagaatctgaaccagcttagcaatactaatagtagatggttcagtaagctgcttccatgtccttgcaactttgcaatgctggcaattttttttcgaaaaagagcaaggctggcaatatgtgtatagcttttgtttggcaatatatgttgcgattctagcgctggtggtatctcaagatcagaaaaagtgagcatcaatgtcttactttattggttttctgactgattacattagtaaacgctgtgtccaaaagtatagagtaatactagttatattttcttaattgtcagtgactcagtatccgcagatggtttgtaaagtgcccttatttttcatggtcgttagctttagtaggtatatagtgtactcttgatggtttatcctttttttcgcccctttctcctttgtcatctgggcgcacgttgctatgcagtttcatgagatggttagtgcgtatccaactattctgtcggcaataaagttccacaatcttcaaaattttctaccattctgatgccaactcctactgcactatcagctcttggtttccagctcagttagtggttagaaattgcagcctacatgaaaaagtaactaatagattttctcgtctccaccttttatagttattttcagtgactcttcttccaaaccaagtggtaaagccaggattctaccattacaatgacgatctgcagctgtgaggagacgatcaatgagtttgagatgttaacgcgtgatgctgcgcgtgtgcagaaggatacactgaaaaagatccttgagataaatgctagtgctgagtacctgcagaactttgggcttggggggaggactgatgccgagagctacaaatcctgcatcccattgtgtgtgcacaatgatattgagccctatatccagcggattgttgatggcgatacctcaccagtggtcactggagaacccatcaccaacctctctctcaggtactgtacccattgcttgaaatttgtcaccttattatgcttatcgtttactagtttatgataataaatgttcatagtctggcttcttctgtggctttcatacagttccggtacaacacacggaaagcccaagttcattccattcaatgatgaattacttgagaccacacttcaaatataccgtacatcttacgcgtttaggaaccggtaaggatctctgatttattattatttatgctcaagtaatgctgctttttctgaatgttcaacaaactttgatgatcaggttcaacatgagttattgtttttatacactgttttgcatgctattaacttgttgcttctttgtgtattttttactctgcatcccagaatattagaataaccttctgttttgtgtttacagtgaataccctattggccaaggaaaagcactgcagtttgtttatggtagcaagcaagtcataacgaaaggtggcattcttgcaacaactgcgacaacaaacttgtataggagacagcgctataaggaaggcatgaaggatatccagtcgcagtgttgcagtcctgatgaagttatctttggccctgatttccaccaatccttgtactgtcacttgctgtgtgggttaatatactcagaggaggtccattcagtgttctcaacatttgctcacagcctagtccatgcattccaaacattcgaggaggtttgggaagatctatgcactgatataagagacggtgttctctcaaagaaagttacagcaccatcaattcgtgaggctgtttcaaaaatcctgaagcccaatcctgaacttgctgactcaatatacaagaagtgtattggtttgagcaattggtatggagtgatcccagcactgtggccaaatgcaaagtatgtgtatggtatcatgacaggatccatggagccatatctgaaaaaattaaggcattacgcagggaacttgccacttataagtgctgactatggcgcatcggaaggatgggttggctctaacatagaccctactgtgccaccagagcaggtgacatatgctgttctcccacaagttggttattttgagttcattcctttggagaaaccaataggggaggagacagaaaactccgcctccattcattacatagaatcagatccagttggcttgactgaagttgaggtcggcaaaatctatgaagttgtaataactaactttgcaggtatattctctcttttctatctattctgaatcttaaattgggttaaatctccattatctagaagtatagcaatgaaaattctacaaaaagcaaggggcagccatccctagttctgcttctggtgctgttatctggtcagacatgataccaacgggatttatcagaaataactagataagtttgtgtgacatattatttcaaaaaagaaatatttattagtcttgccccttcaatcttaaacctctttttgacaagagctccttattatgtaaaaaacataaaaatcacacggttgcaatgtatacatcaatattattaagcacatgggggagtaattattgtgaatgtttgtggtccactccttgaactctttgctgcttactcattatgattatcttaaaactctatcagatgatatatgtttctttttgtgtatgccaaccatgccagactcaccaataacatttttattctttaatatctctggctaacatacaattattttgttttaggacacagaaagtttacttattacggtgatgtttaagaaacactaaaatctttttcttttttctattttgttctaggtctatatcggtacaggctaggagatgtcgtgaagatagcacgcttccacaactccacacctgagctccaattcatctgtcgcagaagcctagtgctgagcatcaacatcgacaagaacaccgagaaagacctccagttggctgttgaggaggcatcaaagttcctggaaggagagaagctcgaagtcatggatttcacgagcttcgtggaacgatcaagcgatccagggcgctacgtgatcttctgggagctgagtggcgatgcgagtgatgaggtcttgagcagctgcgcaaatgccttggatctagccttcattgacgcaggctacacgggctcgaggaagatcaagacgatcggcccccttgagctccggatcctcagaaagggaaccttcaaggagatcctagatcatttcctaagccttggtggtgcggtgagccagttcaagacaccaaggtttgtgaacccatcaaacagcaaggtgttgcagatactgagtagaaatgtcacccaaagttatttcagtactgcctatggattttgatacaaagaaacatattagatgctagcagctgcatgtaattacctttttacctcttgtttttcagctttactgtagtctccaccggaagaaaacgtttggtttagtctcttgaataatctattttcttatcctgtttgtgactatctagaatctatgtaaatggcccagctctagtgaaagttatggtgatgttatcaggaaagaaaatgtggtgtttcccgtaacaagtgctgcaaagtattattcagactc</dnaseqindica>
External Link(s)	NCBI Gene:Os05g0586200, RefSeq:Os05g0586200