Difference between revisions of "Os04g0401000"
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Os04g0401000 is known as Pyricularia oryzae resistance-21('''''Pi-21''''') gene.It is quantitative trait loci (QTLs) conferring field resistance to rice blast. | Os04g0401000 is known as Pyricularia oryzae resistance-21('''''Pi-21''''') gene.It is quantitative trait loci (QTLs) conferring field resistance to rice blast. | ||
==Annotated Information== | ==Annotated Information== | ||
| − | '''''Pi21''''' was identifed in ''Owarihatamochi'' (Japanese upland rice).The resistance gene, designated pi21, was mapped on chromosome 4 as a single recessive gene between RFLP marker loci G271 and G317 at a distance of 5.0 cM and 8.5 cM, respectively,using RFLP and SSR markers.the location of '''''Pi21''''' is showed | + | [[File:QQ截图20140523142047ddddd.jpg|right|thumb|430px|'''''Pi21''''' was identifed in ''Owarihatamochi'' (Japanese upland rice).The resistance gene, designated pi21, was mapped on chromosome 4 as a single recessive gene between RFLP marker loci G271 and G317 at a distance of 5.0 cM and 8.5 cM, respectively,using RFLP and SSR markers.the location of '''''Pi21''''' is showed on the right. The introgressions on chromosome 4 from Owarihatamochi in two BC1 F1 plants, 96BC131 (A) and 96BC122 (B), respectively. The locations of RFLP marker loci and a gene for field resistance, pi21, are indicated with an open ellipse. Genetic distances among them in BC1 F3 populations are shown right on right in centimorgans. The map of rice chromosome 4 is shown on the left, indicating the location of putative QTLs with shaded boxes<ref name="ref1" />. |
| − | + | ||
===Function=== | ===Function=== | ||
| − | '''''Pi21''''' encodes a proline-rich protein that includes a putative heavy metal–binding domain and putative protein-protein interaction motifs. Wild-type '''''Pi21''''' appears to slow the plant’s defense responses, which may support optimization of defense mechanisms. Deletions in its proline-rich motif inhibit this slowing. '''''Pi21''''' is separable from a closely linked gene conferring poor flavor. The resistant pi21 allele, which is found in some strains of japonica rice, could improve blast resistance of rice worldwide. | + | '''''Pi21''''' encodes a proline-rich protein that includes a putative heavy metal–binding domain and putative protein-protein interaction motifs<ref name="ref1" />. Wild-type '''''Pi21''''' appears to slow the plant’s defense responses, which may support optimization of defense mechanisms. Deletions in its proline-rich motif inhibit this slowing<ref name="ref2" />. '''''Pi21''''' is separable from a closely linked gene conferring poor flavor. The resistant pi21 allele, which is found in some strains of japonica rice, could improve blast resistance of rice worldwide<ref name="ref2" />. |
| − | Leaves of AA-pi21, a near-isogenic line (NIL) carrying '''''Pi21''''' in the genetic background of a susceptible cultivar, had smaller lesions than did the susceptible cultivar (AA) in both a field evaluation and a greenhouse inoculation test. The resistant '''''Pi21''''' allele had a consistent effect against all 10 of the widely distributed races of studied, although the resistance was incomplete as compared with that triggered by '''''R''''' genes.Histological examination showed that cells infected with a virulent race were still alive and intact in both AA and AA-pi21 40 hours after inoculation, in contrast to those infected with an avirulent race, which induces '''''R''''' gene–dependent hypersensitive cell death. However, cytoplasmic granules, the first sign of cell death, were frequently observed | + | Leaves of AA-pi21, a near-isogenic line (NIL) carrying '''''Pi21''''' in the genetic background of a susceptible cultivar, had smaller lesions than did the susceptible cultivar (AA) in both a field evaluation and a greenhouse inoculation test. The resistant '''''Pi21''''' allele had a consistent effect against all 10 of the widely distributed races of studied, although the resistance was incomplete as compared with that triggered by '''''R''''' genes.Histological examination showed that cells infected with a virulent race were still alive and intact in both AA and AA-pi21 40 hours after inoculation, in contrast to those infected with an avirulent race, which induces '''''R''''' gene–dependent hypersensitive cell death. However, cytoplasmic granules, the first sign of cell death, were frequently observed in AA-pi21 96 hours after inoculation .The rate of penetration of hyphae into host cells in AA-pi21 did not differ from that in AA, but the rate of invasion of hyphae from penetrated cells into adjacent cells, as an indicator of hyphal growth, was significantly lower in AA-pi21 , which is consistent with the observations in the greenhouse inoculation test. These observations suggest that '''''Pi21''''' confers non–race-specific resistance, which is possibly associated with a slow induction of resistance.The resistant '''''Pi21''''' allele did not affect resistance to other fungal and bacterial pathogens<ref name="ref2" />. |
| − | in AA-pi21 96 hours after inoculation .The rate of penetration of hyphae into host cells in AA-pi21 did not differ from that in AA, but the rate of invasion of hyphae from penetrated cells into adjacent cells, as an indicator of hyphal growth, was significantly lower in AA-pi21 , which is consistent with the observations in the greenhouse inoculation test. These observations suggest that '''''Pi21''''' confers non–race-specific resistance, which is possibly associated with a slow induction of resistance.The resistant '''''Pi21''''' allele did not affect resistance to other fungal and bacterial pathogens. | + | [[File:QQ截图20140523144535.jpg]] |
| − | [[File: | + | [[File:QQ截图20140523145616.jpg]] |
| + | |||
===Expression=== | ===Expression=== | ||
| − | + | The full lenth of cDNA of ｗild-type '''''Pi21''''' is 1109bp, which includes 3 exons，Encoding a proline-rich protein product of 266 amino acids containing heavy metal binding domain and protein-protein interaction domains and other functional domains. Compared with the susceptible cultivar, there are 21bp and 48bp missing of '''''Pi21''''' gene in resistant varieties Owarihatamochi, which is the cause of different resistance<ref name="ref2" />. | |
| − | The full lenth of cDNA of ｗild-type '''''Pi21''''' is 1109bp, which includes 3 exons，Encoding a proline-rich protein product of 266 amino acids containing heavy metal binding domain and protein-protein interaction domains and other functional domains. Compared with the susceptible cultivar, there are 21bp and 48bp missing of '''''Pi21''''' gene in resistant varieties Owarihatamochi, which is the cause of different resistance. | + | [[File:QQ截图20140523150944.jpg]] |
| − | + | [[File:QQ截图20140523151005.jpg]] | |
| − | |||
| − | |||
| − | |||
==Labs working on this gene== | ==Labs working on this gene== | ||
| Line 27: | Line 25: | ||
==References== | ==References== | ||
| + | <references> | ||
| + | <ref name="ref1"> | ||
| + | S.Fukuoka;K.Okuno QTL analysis and mapping of pi21, a recessive gene for field resistance to rice blast in Japanese upland rice Theoretical and Applied Genetics, 2001, 103(2-3): 185-190 | ||
| + | </ref> | ||
| + | <ref name="ref2"> | ||
Shuichi Fukuoka;Norikuni Saka;Hironori Koga;Kazuko Ono;Takehiko Shimizu;Kaworu Ebana;Nagao Hayashi;Akira Takahashi;Hirohiko Hirochika;Kazutoshi Okuno;Masahiro Yano Loss of Function of a Proline-Containing Protein Confers Durable Disease Resistance in Rice Science, 2009, 325(5943): 998-1001 | Shuichi Fukuoka;Norikuni Saka;Hironori Koga;Kazuko Ono;Takehiko Shimizu;Kaworu Ebana;Nagao Hayashi;Akira Takahashi;Hirohiko Hirochika;Kazutoshi Okuno;Masahiro Yano Loss of Function of a Proline-Containing Protein Confers Durable Disease Resistance in Rice Science, 2009, 325(5943): 998-1001 | ||
| − | + | </ref> | |
| + | </references> | ||
==Structured Information== | ==Structured Information== | ||
Revision as of 07:46, 23 May 2014
Os04g0401000 is known as Pyricularia oryzae resistance-21(Pi-21) gene.It is quantitative trait loci (QTLs) conferring field resistance to rice blast.
Contents
Annotated Information
[[File:QQ截图20140523142047ddddd.jpg|right|thumb|430px|Pi21 was identifed in Owarihatamochi (Japanese upland rice).The resistance gene, designated pi21, was mapped on chromosome 4 as a single recessive gene between RFLP marker loci G271 and G317 at a distance of 5.0 cM and 8.5 cM, respectively,using RFLP and SSR markers.the location of Pi21 is showed on the right. The introgressions on chromosome 4 from Owarihatamochi in two BC1 F1 plants, 96BC131 (A) and 96BC122 (B), respectively. The locations of RFLP marker loci and a gene for field resistance, pi21, are indicated with an open ellipse. Genetic distances among them in BC1 F3 populations are shown right on right in centimorgans. The map of rice chromosome 4 is shown on the left, indicating the location of putative QTLs with shaded boxes[1].
Function
Pi21 encodes a proline-rich protein that includes a putative heavy metal–binding domain and putative protein-protein interaction motifs[1]. Wild-type Pi21 appears to slow the plant’s defense responses, which may support optimization of defense mechanisms. Deletions in its proline-rich motif inhibit this slowing[2]. Pi21 is separable from a closely linked gene conferring poor flavor. The resistant pi21 allele, which is found in some strains of japonica rice, could improve blast resistance of rice worldwide[2].
Leaves of AA-pi21, a near-isogenic line (NIL) carrying Pi21 in the genetic background of a susceptible cultivar, had smaller lesions than did the susceptible cultivar (AA) in both a field evaluation and a greenhouse inoculation test. The resistant Pi21 allele had a consistent effect against all 10 of the widely distributed races of studied, although the resistance was incomplete as compared with that triggered by R genes.Histological examination showed that cells infected with a virulent race were still alive and intact in both AA and AA-pi21 40 hours after inoculation, in contrast to those infected with an avirulent race, which induces R gene–dependent hypersensitive cell death. However, cytoplasmic granules, the first sign of cell death, were frequently observed in AA-pi21 96 hours after inoculation .The rate of penetration of hyphae into host cells in AA-pi21 did not differ from that in AA, but the rate of invasion of hyphae from penetrated cells into adjacent cells, as an indicator of hyphal growth, was significantly lower in AA-pi21 , which is consistent with the observations in the greenhouse inoculation test. These observations suggest that Pi21 confers non–race-specific resistance, which is possibly associated with a slow induction of resistance.The resistant Pi21 allele did not affect resistance to other fungal and bacterial pathogens[2].
Expression
The full lenth of cDNA of ｗild-type Pi21 is 1109bp, which includes 3 exons，Encoding a proline-rich protein product of 266 amino acids containing heavy metal binding domain and protein-protein interaction domains and other functional domains. Compared with the susceptible cultivar, there are 21bp and 48bp missing of Pi21 gene in resistant varieties Owarihatamochi, which is the cause of different resistance[2].
Labs working on this gene
1.QTL Genomics Research Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 3058602, Japan. 2.Mountainous Region Agricultural Research Institute, Aichi Agricultural Research Center, Inahasi, Toyota, Aichi 4412513, Japan. 3Bioproduction Sciences, Ishikawa Prefectural University, Suematsu 1-308, Nonoichi-machi, Ishikawa 921-8836, Japan. 4.Institute of the Society for Techno-Innovation of Agriculture, Forestry and Fisheries, Ippaizuka, Tsukuba, Ibaraki 3050854, Japan. 5.Division of Plant Sciences, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 3058602, Japan. 6.Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Kannondai 2-1-2,Tsukuba, Ibaraki 305-8602, Japan. 7.Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennohdai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
References
- ↑ 1.0 1.1 S.Fukuoka;K.Okuno QTL analysis and mapping of pi21, a recessive gene for field resistance to rice blast in Japanese upland rice Theoretical and Applied Genetics, 2001, 103(2-3): 185-190
- ↑ 2.0 2.1 2.2 2.3 Shuichi Fukuoka;Norikuni Saka;Hironori Koga;Kazuko Ono;Takehiko Shimizu;Kaworu Ebana;Nagao Hayashi;Akira Takahashi;Hirohiko Hirochika;Kazutoshi Okuno;Masahiro Yano Loss of Function of a Proline-Containing Protein Confers Durable Disease Resistance in Rice Science, 2009, 325(5943): 998-1001
Structured Information
| Gene Name |
Os04g0401000 |
|---|---|
| Description |
Heavy metal transport/detoxification protein domain containing protein |
| Version |
NM_001059220.1 GI:115458169 GeneID:4335725 |
| Length |
1679 bp |
| Definition |
Oryza sativa Japonica Group Os04g0401000, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 4:19856181..19857859 |
| Sequence Coding Region |
19856474..19856539,19856708..19857192,19857329..19857410 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008397:19856181..19857859 source=RiceChromosome04 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008397:19856181..19857859 source=RiceChromosome04 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgggtatattggtcatcttggtggacctgcaatgctgccgctgcgatgccaagatcaggaaggtcctgggctgccttgaagaggagtactgcatcgagaaggtggagtacgacgtgaagaacaacagggtgatcgtgcgcgggaagttcgacccggagaagctgtgcaagaagatctggtgcaaggccggcaagatcatcaaggagatcctcatcgtcgacgtctggccgccgccgctgccgcagccgccgccgccgtgcaagccgccgccgtgcgagaagcctccggaggactgcaagcccaagccctgccattgctgcagctgcgagaagcccaagcccaagcccaagccctgccactgcgagaagcccaagccctgtcactgcgagaagcccaagccatgcgagaagccgccgccgtgcaagccggaggagccgccgaagccgccgccggagaagccgccgccgaagccggagtgcaagctggtgccgtacccttacccggtgccgtacccgtacgccgggcagtggtgctgcccaaagcctgagccgccgaagccgccgccgcccgtctgcccgccgccgccgtggtgctacaccgaggacaacgccaacgcctgctccatcatgtga</cdnaseq> |
| Protein Sequence |
<aaseq>MGILVILVDLQCCRCDAKIRKVLGCLEEEYCIEKVEYDVKNNRV IVRGKFDPEKLCKKIWCKAGKIIKEILIVDVWPPPLPQPPPPCKPPPCEKPPEDCKPK PCHCCSCEKPKPKPKPCHCEKPKPCHCEKPKPCEKPPPCKPEEPPKPPPEKPPPKPEC KLVPYPYPVPYPYAGQWCCPKPEPPKPPPPVCPPPPWCYTEDNANACSIM</aaseq> |
| Gene Sequence |
<dnaseqindica>1321..1386#668..1152#450..531#atgcatcgcctccattattcatgcttcgattctccatgctttcctctactgctcattggtaacattcggcaaatttgacaggtgagctcagtattttaaatcttaatgtagtactttggtgtgctaatctttgctctgttcaaaagagaattctggtttctttgctattttgaaaagagaattttcgttacaggacttcaacttccataatacttttttttataattaaggcatgatatatatcttctttctgaattccacgggaattgcactttttcctgagaaatttgtaaagagcatgcctgttaattgcaaggggcccctaactctgttatgagaaaagagaactatataagatgctcaataagcacctctttttttttttctgttaactgaccaaagcctgtctatctgcatttttttttgttttttgtttttcttgtgtgcagatgggtatattggtcatcttggtggacctgcaatgctgccgctgcgatgccaagatcaggaaggtcctgggctgccttgaaggtataataaattctgcccgaatcgtccatgtttgattgaattttcaaggctaatcagcagtgttcctgctcaattgggagcaaaacctctgttaaaaagggtgtgtttgaatgaatataattgaatatgaacgcagaggagtactgcatcgagaaggtggagtacgacgtgaagaacaacagggtgatcgtgcgcgggaagttcgacccggagaagctgtgcaagaagatctggtgcaaggccggcaagatcatcaaggagatcctcatcgtcgacgtctggccgccgccgctgccgcagccgccgccgccgtgcaagccgccgccgtgcgagaagcctccggaggactgcaagcccaagccctgccattgctgcagctgcgagaagcccaagcccaagcccaagccctgccactgcgagaagcccaagccctgtcactgcgagaagcccaagccatgcgagaagccgccgccgtgcaagccggaggagccgccgaagccgccgccggagaagccgccgccgaagccggagtgcaagctggtgccgtacccttacccggtgccgtacccgtacgccgggcagtggtgctgcccaaagcctgagccgccgaagccgccgccggagccaccgaaggagccggagccgccgaagccgtgcgggtgctcgcacgccttcgtgtgcgtctgcaagccggcgccgccgccgccgccgccgtgcgggtgctcggggggccacgggaactgcggctgcggcatcaggccgtggccgccgcaggtgtggccgccgccgcccgtctgcccgccgccgccgtggtgctacaccgaggacaacgccaacgcctgctccatcatgtgatggccggccggcggtcggcgtcgatcacgatcatctctgctgcttaatttccttgcttgctactacctctgctcctttccttgcctcggaaatcggaataaattaaacacgaggctgatcgatgtgtttgtaattaatccatggtgtttgtgttgtgtgctgtgtgggctgtataataattaattacagtatgttcatgtaaatttgtttgtttgtttgtttatgttgttcgatatgtataattatgtacaataattaatcgtggagaggctcactaaactcataaactgtag</dnaseqindica> |
| External Link(s) |
