Difference between revisions of "Os03g0111300"
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NsLTPs bind to a variety of lipid molecules and catalyze their transfer across membranes in vitro [2]. NsLTPs also have additional biological functions, including biosynthesis of cutin, involvement in defense against pathogens, and managing abiotic stress conditions imparted by temperature or drought [2] and [3]. The nsLTP superfamily possesses eight highly conserved cysteine residues forming four disulfide bonds [1] and [4]. NsLTPs are subdivided into two subfamilies that differ in molecular mass, nsLTP1 (9 kDa), and nsLTP2 (7 kDa) [1]. | NsLTPs bind to a variety of lipid molecules and catalyze their transfer across membranes in vitro [2]. NsLTPs also have additional biological functions, including biosynthesis of cutin, involvement in defense against pathogens, and managing abiotic stress conditions imparted by temperature or drought [2] and [3]. The nsLTP superfamily possesses eight highly conserved cysteine residues forming four disulfide bonds [1] and [4]. NsLTPs are subdivided into two subfamilies that differ in molecular mass, nsLTP1 (9 kDa), and nsLTP2 (7 kDa) [1]. | ||
| − | + | Rice seeds (100 g) suspended in 800 ml of 50 mM sulfuric acid were stirred for 4 h. The suspension was centrifuged at 9000g for 20 min to remove flocculent sediments. Supernatant was kept at 4 °C for 12 h, after adjusting the pH to 7. A clear solution was loaded onto a Sephadex column (C-25), prewashed with two bed volumes of 25 mM sodium phosphate buffer (pH 7.2). Low-affinity proteins were eluted with the same wash buffer. The proteins of interest, eluted with wash buffer containing 1 M NaCl, were desalted and concentrated using an Amicon concentrator. The concentrated protein solution was loaded onto a Source 15S cation exchange column (Pharmacia) to elute nsLTPs using a 0.2–0.3 M NaCl gradient. NsLTPs were separated with a reverse-phase HPLC using C18 semi-preparative column (Cosmosil) to get pure rice nsLTP2. The mobile phase used was acetonitrile containing 0.1% (v/v) trifluoroacetic acid. NsLTP2 was eluted at 20–25% acetonitrile while using linear acetonitrile–water gradient.[5] | |
| − | |||
===Evolution=== | ===Evolution=== | ||
Revision as of 13:06, 23 May 2014
Please input one-sentence summary here.
==Annotated Informatio
NsLTPs bind to a variety of lipid molecules and catalyze their transfer across membranes in vitro [2]. NsLTPs also have additional biological functions, including biosynthesis of cutin, involvement in defense against pathogens, and managing abiotic stress conditions imparted by temperature or drought [2] and [3]. The nsLTP superfamily possesses eight highly conserved cysteine residues forming four disulfide bonds [1] and [4]. NsLTPs are subdivided into two subfamilies that differ in molecular mass, nsLTP1 (9 kDa), and nsLTP2 (7 kDa) [1].
Rice seeds (100 g) suspended in 800 ml of 50 mM sulfuric acid were stirred for 4 h. The suspension was centrifuged at 9000g for 20 min to remove flocculent sediments. Supernatant was kept at 4 °C for 12 h, after adjusting the pH to 7. A clear solution was loaded onto a Sephadex column (C-25), prewashed with two bed volumes of 25 mM sodium phosphate buffer (pH 7.2). Low-affinity proteins were eluted with the same wash buffer. The proteins of interest, eluted with wash buffer containing 1 M NaCl, were desalted and concentrated using an Amicon concentrator. The concentrated protein solution was loaded onto a Source 15S cation exchange column (Pharmacia) to elute nsLTPs using a 0.2–0.3 M NaCl gradient. NsLTPs were separated with a reverse-phase HPLC using C18 semi-preparative column (Cosmosil) to get pure rice nsLTP2. The mobile phase used was acetonitrile containing 0.1% (v/v) trifluoroacetic acid. NsLTP2 was eluted at 20–25% acetonitrile while using linear acetonitrile–water gradient.[5]
Evolution
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Labs working on this gene
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[1]J.P Douliez, T Michon, K Elmorjani, D Marion Structure, biological and technological functions of lipid transfer proteins and indolines, the major lipid binding proteins from cereal kernels J. Cereal Sci., 32 (2000), pp. 1–20
[2]J.C Kader Lipid transfer proteins in plants Annu. Rev. Plant Physiol. Plant Mol. Biol., 47 (1996), pp. 627–654
[3]K.L Larsen, J.R Winther Surprisingly high stability of barley lipid transfer protein, LTP1, towards denaturant, heat, and proteases FEBS Lett., 488 (2001), pp. 145–148
[4]J.P Douliez, C Pato, H Rabesona, D Molle, D Marion Disulfide bond assignment, lipid transfer activity and secondary structure of a 7-kDa plant lipid transfer protein, LTP2 Eur. J. Biochem., 268 (2001), pp. 1400–1403
Structured Information
| Gene Name |
Os03g0111300 |
|---|---|
| Description |
Nonspecific lipid-transfer protein 2 (nsLTP2) (7 kDa lipid transfer protein) |
| Version |
NM_001055258.1 GI:115450244 GeneID:4331362 |
| Length |
476 bp |
| Definition |
Oryza sativa Japonica Group Os03g0111300, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 3:630670..631145 |
| Sequence Coding Region |
630809..631099 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008396:630670..631145 source=RiceChromosome03 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008396:630670..631145 source=RiceChromosome03 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgatgaggaagttggcggtgttggtgttggcggtggcgatggtggcggcgtgcggcggcggcgtcgtgggtgtagcgggggccggttgcaacgctgggcagctgacggtgtgcacgggggcgatcgcgggcggggcgcggccgacggcggcgtgctgctccagcctgcgggcgcagcagggctgcttctgccagttcgccaaggacccgcgctacgggcgctacgtcaacagccccaacgcccgcaaggccgtctcctcctgcggcatcgccctccccacctgccactga</cdnaseq> |
| Protein Sequence |
<aaseq>MMRKLAVLVLAVAMVAACGGGVVGVAGAGCNAGQLTVCTGAIAG GARPTAACCSSLRAQQGCFCQFAKDPRYGRYVNSPNARKAVSSCGIALPTCH</aaseq> |
| Gene Sequence |
<dnaseqindica>47..337#gtacctcgcagcaccaagctagctagcttcgatcagtagctggaggatgatgaggaagttggcggtgttggtgttggcggtggcgatggtggcggcgtgcggcggcggcgtcgtgggtgtagcgggggccggttgcaacgctgggcagctgacggtgtgcacgggggcgatcgcgggcggggcgcggccgacggcggcgtgctgctccagcctgcgggcgcagcagggctgcttctgccagttcgccaaggacccgcgctacgggcgctacgtcaacagccccaacgcccgcaaggccgtctcctcctgcggcatcgccctccccacctgccactgatccatccatccatcgtctcctactcttttattttgtgatgtggtgtacgtgttcgtagtattgtcttggcgtcatctcgtcacgacgcgtatatgcatgcgcagtgcggcttttgaataaaaggggatcgaccgatgtt</dnaseqindica> |
| External Link(s) |
