Difference between revisions of "Os06g0133000"
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Carbohydrate analysis indicated that the content of amylose in GM077 seeds was significantly reduced, while that of amylopectin significantly rose as compared to the wild type BP034. The content of glucose, sucrose, total starch, cell-wall polysaccharides and oil were only slightly affected in the mutant as compared to the wild type. Suppression subtractive hybridization (SSH) experiments generated 116 unigenes in the mutant on the wild-type background. Among the 116 unigenes, three, AGP, ISA1 and SUSIBA2-like, were found to be directly involved in amylopectin synthesis, indicating their possible roles in redirecting carbon flux from amylose to amylopectin. A bioinformatics analysis of the putative SUSIBA2-like binding elements in the promoter regions of the upregulated genes indicated that the SUSIBA2-like transcription factor may be instrumental in promoting the carbon reallocation from amylose to amylopectin. | Carbohydrate analysis indicated that the content of amylose in GM077 seeds was significantly reduced, while that of amylopectin significantly rose as compared to the wild type BP034. The content of glucose, sucrose, total starch, cell-wall polysaccharides and oil were only slightly affected in the mutant as compared to the wild type. Suppression subtractive hybridization (SSH) experiments generated 116 unigenes in the mutant on the wild-type background. Among the 116 unigenes, three, AGP, ISA1 and SUSIBA2-like, were found to be directly involved in amylopectin synthesis, indicating their possible roles in redirecting carbon flux from amylose to amylopectin. A bioinformatics analysis of the putative SUSIBA2-like binding elements in the promoter regions of the upregulated genes indicated that the SUSIBA2-like transcription factor may be instrumental in promoting the carbon reallocation from amylose to amylopectin. | ||
| − | ===Expression=== | + | [[File:Example.jpg]]===Expression=== |
Please input expression information here. | Please input expression information here. | ||
Analyses of carbohydrate and oil fractions and gene expression profiling on a global scale in the rice waxy mutant GM077 revealed several candidate genes implicated in the carbon reallocation response to an amylose deficiency, including genes encoding AGPase and SUSIBA2-like. We believe that AGP and SUSIBA2 are two promising targets for classical breeding and/or transgenic plant improvement to control the carbon flux between starch and other components in cereal seeds | Analyses of carbohydrate and oil fractions and gene expression profiling on a global scale in the rice waxy mutant GM077 revealed several candidate genes implicated in the carbon reallocation response to an amylose deficiency, including genes encoding AGPase and SUSIBA2-like. We believe that AGP and SUSIBA2 are two promising targets for classical breeding and/or transgenic plant improvement to control the carbon flux between starch and other components in cereal seeds | ||
Revision as of 16:38, 23 May 2014
Please input one-sentence summary here.
Contents
Annotated Information
Function
Please input function information here. Carbohydrate analysis indicated that the content of amylose in GM077 seeds was significantly reduced, while that of amylopectin significantly rose as compared to the wild type BP034. The content of glucose, sucrose, total starch, cell-wall polysaccharides and oil were only slightly affected in the mutant as compared to the wild type. Suppression subtractive hybridization (SSH) experiments generated 116 unigenes in the mutant on the wild-type background. Among the 116 unigenes, three, AGP, ISA1 and SUSIBA2-like, were found to be directly involved in amylopectin synthesis, indicating their possible roles in redirecting carbon flux from amylose to amylopectin. A bioinformatics analysis of the putative SUSIBA2-like binding elements in the promoter regions of the upregulated genes indicated that the SUSIBA2-like transcription factor may be instrumental in promoting the carbon reallocation from amylose to amylopectin.
===Expression===
Please input expression information here.
Analyses of carbohydrate and oil fractions and gene expression profiling on a global scale in the rice waxy mutant GM077 revealed several candidate genes implicated in the carbon reallocation response to an amylose deficiency, including genes encoding AGPase and SUSIBA2-like. We believe that AGP and SUSIBA2 are two promising targets for classical breeding and/or transgenic plant improvement to control the carbon flux between starch and other components in cereal seeds
Evolution
Please input evolution information here.
Waxy mutants of cereals have a high content of amylopectin and have been well characterized. However, the allocation of carbon to other components, such as beta-glucan and oils, and the regulation of the altered carbon distribution to amylopectin in a waxy mutant are poorly understood. In this study, we used a rice mutant, GM077, with a low content of amylose to gain molecular insight into how a deficiency of amylose affects carbon allocation to other end products and to amylopectin. We used carbohydrate analysis, subtractive cDNA libraries, and qPCR to identify candidate genes potentially responsible for the changes in carbon allocation in GM077 seeds
Labs working on this gene
Please input related labs here. 1.State Key Laboratory of Rice Biology, China National Rice Research Institute, 31006 Hangzhou, China
2.Department of Plant Biology & Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, P.O. Box 7080, SE 75007, Uppsala, Sweden
References
Please input cited references here. 1. Ming-Zhou Zhang;Jie-Hong Fang;Xia Yan;Jun Liu;Jin-Song Bao;Gunnel Fransson;Roger Andersson;Christer Jansson;Per Åman;Chuanxin Sun
Molecular insights into how a deficiency of amylose affects carbon allocation -- carbohydrate and oil analyses and gene expression profiling in the seeds of a rice waxy mutant BMC Plant Biology, 2012, 12: 230
2. Yan Su;Yuchun Rao;Shikai Hu;Yaolong Yang;Zhenyu Gao;Guanghen Zhang;Jian Liu;Jiang Hu;Meixian Yan;Guojun Dong;Li Zhu;Longbiao Guo;Qian Qian;Dali Zeng
Map-based cloning proves qGC-6, a major QTL for gel consistency of japonica/indica cross, responds by Waxy in rice (Oryza sativa L.) Theoretical and Applied Genetics, 2011, 123(5): 859-867
3. 姚姝;陈涛;张亚东;朱镇;赵凌;赵庆勇;周丽慧;王才林
分子标记辅助选择聚合水稻暗胚乳突变基因Wx-mq和抗条纹叶枯病基因Stv-bi 中国水稻科学, 2010, 24(4): 341-347
4. Zhixi Tian;Qian Qian;Qiaoquan Liu;Meixian Yan;Xinfang Liu;Changjie Yan;Guifu Liu;Zhenyu Gao;Shuzhu Tang;Dali Zeng;Yonghong Wang;Jianming Yu;Minghong Gu;Jiayang Li
Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities Proceedings of the National Academy of Sciences, 2009, 106(51): 21760-21765
5. P. Jayamani;S. Negrão;C. Brites;M.M. Oliveira
Potential of Waxy gene microsatellite and single-nucleotide polymorphisms to develop japonica varieties with desired amylose levels in rice (Oryza sativa L.) Journal of Cereal Science, 2007, 46(2): 178-186
6. 张娅丽; 许明辉; 曾亚文; 姚春馨; 陈善娜
云南地方稻种Wx基因第一内含子供体+1位碱基变异与直链淀粉含量的关系 中国水稻科学, 2007, 21(1): 20-24
7. 刘玉花; 栾丽; 龙文波; 王兴; 孔繁伦; 何涛; 涂升斌
同源四倍体和二倍体水稻Wx基因与淀粉品质的遗传关系 中国水稻科学, 2007, 21(2): 143-149
8. WAN Ying-xiu;DENG Qi-ming;WANG Shi-quan;LIU Ming-wei;ZHOU Hua-qiang;LI Ping
Genetic Polymorphism of Wx Gene and Its Correlation with Main Grain Quality Characteristics in Rice Rice Science, 2007, 14(2): 85-93
9. 陈刚; 王忠; 刘巧泉; 熊飞; 顾蕴洁; 顾国俊
转反义Wx基因水稻颖果的发育及物质积累 中国水稻科学, 2006, 20(3): 277-282
10. 万映秀;邓其明;王世全;刘明伟;周华强;李平
水稻Wx基因的遗传多态性及其与主要米质指标的相关性分析 中国水稻科学, 2006, 20(6): 603-609
11. CHEN Gang; WANG Zhong; LIU Qiao-quan; XIONG Fei; GU Yun-jie; GU Guo-jun
Development and Substance Accumulation of Caryopsis in Transgenic Rice with Antisense Wx Gene Rice Science, 2006, 13(2): 106-112
12. ZENG Rui-zhen; ZHANG Ze-min; HE Feng-hua; XI Zhang-ying; Akshay TALUKDAR; SHI Jun-qiong; QIN Li-jun; HUANG Chao-feng; ZHANG Gui-quan
Identification of Multiple Alleles at the Wx Locus and Development of Single Segment Substitution Lines for the Alleles in Rice Rice Science, 2006, 13(1): 9-14
13. 曾瑞珍; 张泽民; 何风华; 席章营; Akshay TALUKDAR; 施军琼; 秦利军; 黄朝锋; 张桂权
水稻Wx复等位基因的鉴定及单片段代换系的建立 中国水稻科学, 2005, 19(6): 495-500
14. MAO Xing-xue; LIU Yan-zhuo; XIAO Xin; CHEN Jian-wei; LUO Wen-yong; LI Xiao-fang
A One-Step PCR Method for Detecting the First Base of Splice Donor of Wx Intron 1 in Rice Rice Science, 2004, 11(5-6): 342-344
15. Yamanaka-S;Nakamura-I;Watanabe-K-N;Sato-Y-I
Identification of SNPs in the waxy gene among glutinous rice cultivars and their evolutionary significance during the domestication process of rice Theoretical and Applied Genetics, 2004, 108(7): 1200-1204
16. Zhongyi Li;Fei Sun;Shoumin Xu;Xiusheng Chu;Y. Mukai;M. Yamamoto;Shahjahan Ali;Lynette Rampling;Behjat Kosar-Hashemi;Sadequr Rahman;Matthew K. Morell
The structural organisation of the gene encoding class II starch synthase of wheat and barley and the evolution of the genes encoding starch synthases in plants Functional & Integrative Genomics, 2003, 3(1-2): 76-85
17. Y. Sato;T. Nishio
Mutation detection in rice waxy mutants by PCR-RF-SSCP Theoretical and Applied Genetics, 2003, 107: 560-567
18. Hiroyuki Sato; Yasuhiro Suzuki; Makoto Sakai and Tokio Imbe
Molecular Characterization of Wx-mq, a Novel Mutant Gene for Low-amylose Content in Endosperm of Rice (Oryza sativa L.) Breeding Science, 2002, 52(2): 131-135
19. 于恒秀; 刘巧泉; 陈秀花; 陆美芳; 王兴稳; 王宗阳; 顾铭洪
根癌农杆菌介导的水稻转化系统的优化及转反义Wx基因植株的获得 中国水稻科学, 2002, 16(4): 304-310
20. Ichiho Mikami;Munetoshi Aikawa;Hiro-Yuki Hirano and Yoshio Sano
Altered tissue-specific expression at the Wx gene of the opaque mutants in rice Euphytica, 1999, 105(2): 91-97
21. Hiro-Yuki Hirano;Mitsugu Eiguchi;Yoshio Sano
A single base change altered the regulation of the Waxy gene at the posttranscriptional level during the domestication of rice Molecular Biology and Evolution, 1998, 15(8): 978-987
22. Masayuki Isshiki;Kazuko Morino;Midori Nakajima;Ron J. Okagaki;Susan R. Wessler;Takeshi Izawa;Ko Shimamoto
A naturally occurring functional allele of the rice waxy locus has a GT to TT mutation at the 5' splice site of the first intron The Plant Journal, 1998, 15(1): 133-138
23. N. M. Ayres;A. M. McClung;P. D. Larkin;H. F. J. Bligh;C. A. Jones;W. D. Park
Microsatellites and a single-nucleotide polymorphism differentiate apparentamylose classes in an extended pedigree of US rice germ plasm Theoretical and Applied Genetics, 1997, 94(6-7): 773-781
24. Zong-Yang Wang;Fei-Qin Zheng;Ge-Zhi Shen;Ji-Ping Gao;D. Peter Snustad;Min-Gang Li;Jing-Liu Zhang;Meng-Min Hong
The amylose content in rice endosperm is related to the post-transcriptional regulation of the waxy gene The Plant Journal, 1995, 7(4): 613-622
25. 何祖华; 华金渭; 申宗坦
水稻wx基因对籽粒糖类积累和萌发种子淀粉酶活力的影响 中国水稻科学, 1994, 8(1): 21-26
26. 郑柔; 奚永安; 林贻滋
利用水稻糯与非糯(近)等基因系探讨WX基因对成熟胚培养力的影响 中国水稻科学, 1993, 7(1): 7-10
27. Zong-yang Wang; Zhi-liang Wu; Yan-yan Xing; Fei-gin Zheng; Xiao-Ii Guo; Wei-guo Zhang and Meng-min Hong
Nucleotide sequence of rice waxy gene Nucleic Acids Research, 1990, 18(19): 5898-
28. Yoshio Sano;Mitsuko Katsumata;Kazutoshi Okuno
Genetic studies of speciation in cultivated rice. 5. Inter- and intraspecific differentiation in the waxy gene expression of rice Euphytica, 1986, 35(1): 1-9
29. Y. Sano;M. Maekawa;H. Kikuchl
Temperature effects on the Wx protein level and amylose content in the endosperm of rice Journal of Heredity, 1985, 76(3): 221-222
30. Yoshio Sano
Differential regulation of waxy gene expression in rice endosperm Theoretical and Applied Genetics, 1984, 68(5): 467-473
Structured Information
| Gene Name |
Os06g0133000 |
|---|---|
| Description |
Granule-bound starch synthase I, chloroplast precursor (EC 2.4.1.21) |
| Version |
NM_001063239.1 GI:115466209 GeneID:4340018 |
| Length |
4953 bp |
| Definition |
Oryza sativa Japonica Group Os06g0133000, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 6:1764623..1769575 |
| Sequence Coding Region |
1765922..1766260,1766374..1766454,1766562..1766660,1766757..1766846,1766946..1767009 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008399:1764623..1769575 source=RiceChromosome06 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008399:1764623..1769575 source=RiceChromosome06 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgtcggctctcaccacgtcccagctcgccacctcggccaccggcttcggcatcgccgacaggtcggcgccgtcgtcgctgctccgccacgggttccagggcctcaagccccgcagccccgccggcggcgacgcgacgtcgctcagcgtgacgaccagcgcgcgcgcgacgcccaagcagcagcggtcggtgcagcgtggcagccggaggttcccctccgtcgtcgtgtacgccaccggcgccggcatgaacgtcgtgttcgtcggcgccgagatggccccctggagcaagaccggcggcctcggtgacgtcctcggtggcctcccccctgccatggctgcgaatggccacagggtcatggtgatctctcctcggtacgaccagtacaaggacgcttgggataccagcgttgtggctgagatcaaggttgcagacaggtacgagagggtgaggtttttccattgctacaagcgtggagtcgaccgtgtgttcatcgaccatccgtcattcctggagaaggtttggggaaagaccggtgagaagatctacggacctgacactggagttgattacaaagacaaccagatgcgtttcagccttctttgccaggcagcactcgaggctcctaggatcctaaacctcaacaacaacccatacttcaaaggaacttatggtgaggatgttgtgttcgtctgcaacgactggcacactggcccactggcgagctacctgaagaacaactaccagcccaatggcatctacaggaatgcaaaggttgctttctgcatccacaacatctcctaccagggccgtttcgctttcgaggattaccctgagctgaacctctccgagaggttcaggtcatccttcgatttcatcgacgggtatgacacgccggtggagggcaggaagatcaactggatgaaggccggaatcctggaagccgacagggtgctcaccgtgagcccgtactacgccgaggagctcatctccggcatcgccaggggatgcgagctcgacaacatcatgcggctcaccggcatcaccggcatcgtcaacggcatggacgtcagcgagtgggatcctagcaaggacaagtacatcaccgccaagtacgacgcaaccacggcaatcgaggcgaaggcgctgaacaaggaggcgttgcaggcggaggcgggtcttccggtcgacaggaaaatcccactgatcgcgttcatcggcaggctggaggaacagaagggccctgacgtcatggccgccgccatcccggagctcatgcaggaggacgtccagatcgttcttctgggtactggaaagaagaagttcgagaagctgctcaagagcatggaggagaagtatccgggcaaggtgagggccgtggtgaagttcaacgcgccgcttgctcatctcatcatggccggagccgacgtgctcgccgtccccagccgcttcgagccctgtggactcatccagctgcaggggatgagatacggaacgccctgtgcttgcgcgtccaccggtgggctcgtggacacggtcatcgaaggcaagactggtttccacatgggccgtctcagcgtcgactgcaaggtggtggagccaagcgacgtgaagaaggtggcggccaccctgaagcgcgccatcaaggtcgtcggcacgccggcgtacgaggagatggtcaggaactgcatgaaccaggacctctcctggaaggggcctgcgaagaactgggagaatgtgctcctgggcctgggcgtcgccggcagcgcgccggggatcgaaggcgacgagatcgcgccgctcgccaaggagaacgtggctgctccttga</cdnaseq> |
| Protein Sequence |
<aaseq>MSALTTSQLATSATGFGIADRSAPSSLLRHGFQGLKPRSPAGGD ATSLSVTTSARATPKQQRSVQRGSRRFPSVVVYATGAGMNVVFVGAEMAPWSKTGGLG DVLGGLPPAMAANGHRVMVISPRYDQYKDAWDTSVVAEIKVADRYERVRFFHCYKRGV DRVFIDHPSFLEKVWGKTGEKIYGPDTGVDYKDNQMRFSLLCQAALEAPRILNLNNNP YFKGTYGEDVVFVCNDWHTGPLASYLKNNYQPNGIYRNAKVAFCIHNISYQGRFAFED YPELNLSERFRSSFDFIDGYDTPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIA RGCELDNIMRLTGITGIVNGMDVSEWDPSKDKYITAKYDATTAIEAKALNKEALQAEA GLPVDRKIPLIAFIGRLEEQKGPDVMAAAIPELMQEDVQIVLLGTGKKKFEKLLKSME EKYPGKVRAVVKFNAPLAHLIMAGADVLAVPSRFEPCGLIQLQGMRYGTPCACASTGG LVDTVIEGKTGFHMGRLSVDCKVVEPSDVKKVAATLKRAIKVVGTPAYEEMVRNCMNQ DLSWKGPAKNWENVLLGLGVAGSAPGIEGDEIAPLAKENVAAP</aaseq> |
| Gene Sequence |
<dnaseqindica>1300..1638#1752..1832#1940..2038#2135..2224#2324..2387#2480..2580#2671..2780#2902..3145#3263..3439#3683..3874#3981..4067#4177..4305#4663..4779#accattccttcagttctttgtctatctcaagacacaaataactgcagtctctctctctctctctctctctctctctctctctctgcttcacttctctgcttgtgttgttctgttgttcatcaggaagaacatctgcaagttatacatatatgtttataattctttgtttcccctcttattcagatcgatcacatgcatctttcattgctcgtttttccttacaagtagtctcatacatgctaatttctgtaaggtgttgggctggaaattaattaattaattaattgacttgccaagatccatatatatgtcctgatattaaatcttcgttcgttatgtttggttaggctgatcaatgttattctagagtctagagaaacacacccaggggttttccaactagctccacaagatggtgggctagctgacctagatttgaagtctcactccttataattattttatattagatcattttctaatattcgtgtctttttttattctagagtctagatcttgtgttcaactctcgttaaatcatgtctctcgccactggagaaacagatcaggagggtttattttgggtataggtcaaagctaagattgaaattcacaaatagtaaaatcagaatccaaccaattttagtagccgagttggtcaaaggaaaatgtatatagctagatttattgttttggcaaaaaaaaatctgaatatgcaaaatacttgtatatctttgtattaagaagatgaaaataagtagcagaaaattaaaaaatggattatatttcctgggctaaaagaattgttgatttggcacaattaaattcagtgtcaaggttttgtgcaagaattcagtgtgaaggaatagattctcttcaaaacaatttaatcattcatctgatctgctcaaagctctgtgcatctccgggtgcaacggccaggatatttattgtgcagtaaaaaaatgtcatatcccctagccacccaagaaactgctccttaagtccttataagcacatatggcattgtaatatatatgtttgagttttagcgacaatttttttaaaaacttttggtcctttttatgaacgttttaagtttcactgtctttttttttcgaattttaaatgtagcttcaaattctaatccccaatccaaattgtaataaacttcaattctcctaattaacatcttaattcatttatttgaaaaccagttcaaattcttttaggctcaccaaaccttaaacaattcaattcagtgcagagatcttccacagcaacagctagacaaccaccatgtcggctctcaccacgtcccagctcgccacctcggccaccggcttcggcatcgccgacaggtcggcgccgtcgtcgctgctccgccacgggttccagggcctcaagccccgcagccccgccggcggcgacgcgacgtcgctcagcgtgacgaccagcgcgcgcgcgacgcccaagcagcagcggtcggtgcagcgtggcagccggaggttcccctccgtcgtcgtgtacgccaccggcgccggcatgaacgtcgtgttcgtcggcgccgagatggccccctggagcaagaccggcggcctcggtgacgtcctcggtggcctcccccctgccatggctgtaagcacacacaaacttcgatcgctcgtcgtcgctgaccgtcgtcgtcttcaactgttcttgatcatcgcattggatggatgtgtaatgttgtgttcttgtgttctttgcaggcgaatggccacagggtcatggtgatctctcctcggtacgaccagtacaaggacgcttgggataccagcgttgtggctgaggtaggagcatatgcgtgatcagatcatcacaagatcgattagctttagatgatttgttacatttcgcaagattttaacccaagtttttgtggtgcaattcattgcagatcaaggttgcagacaggtacgagagggtgaggtttttccattgctacaagcgtggagtcgaccgtgtgttcatcgaccatccgtcattcctggagaaggtggagtcatcattagtttaccttttttgtttttactgaattattaacagtgcatttagcagttggactgagcttagcttccactggtgatttcaggtttggggaaagaccggtgagaagatctacggacctgacactggagttgattacaaagacaaccagatgcgtttcagccttctttgccaggtcagtgattacttctatctgatgatggttggaagcatcacgagtttaccatagtatgtatggattcataactaattcgtgtattgatgctacctgcaggcagcactcgaggctcctaggatcctaaacctcaacaacaacccatacttcaaaggaacttatggtgagttacaattgatctcaagatcttataactttcttcgaaggaatccatgatgatcagactaattccttccggtttgttactgacaacaggtgaggatgttgtgttcgtctgcaacgactggcacactggcccactggcgagctacctgaagaacaactaccagcccaatggcatctacaggaatgcaaaggtctatgcttgttcttgccataccaactcaaatctgcatgcacactgcattctgttcagaaactgactgtctgaatctttttcactgcaggttgctttctgcatccacaacatctcctaccagggccgtttcgctttcgaggattaccctgagctgaacctctccgagaggttcaggtcatccttcgatttcatcgacgggtatgagtaagattctaagagtaacttactgtcaattcgccatatatcgattcaatccaagatccttttgagctgacaaccctgcactactgtccatcgttcaaatccggttaaatttcaggtatgacacgccggtggagggcaggaagatcaactggatgaaggccggaatcctggaagccgacagggtgctcaccgtgagcccgtactacgccgaggagctcatctccggcatcgccaggggatgcgagctcgacaacatcatgcggctcaccggcatcaccggcatcgtcaacggcatggacgtcagcgagtgggatcctagcaaggacaagtacatcaccgccaagtacgacgcaaccacggtaagaacgaatgcattcttcacaagatatgcaatctgaattttctttgaaaaagaaattatcatctgtcacttcttgattgattctgacaaggcaagaatgagtgacaaatttcaggcaatcgaggcgaaggcgctgaacaaggaggcgttgcaggcggaggcgggtcttccggtcgacaggaaaatcccactgatcgcgttcatcggcaggctggaggaacagaagggccctgacgtcatggccgccgccatcccggagctcatgcaggaggacgtccagatcgttcttctggtataatataatacactacaagacacacttgcacgatatgccaaaaattcagaacaaattcagtggcaaaaaaaaaactcgaatattagggaaggacctaataatatcaaataattagaaggggtgaggctttgaacccagatcgtctagtccaccaccttgtggagttagccggaagacctctgagcatttctcaattcagtggcaaatgatgtgtataattttgatccgtgtgtgtttcagggtactggaaagaagaagttcgagaagctgctcaagagcatggaggagaagtatccgggcaaggtgagggccgtggtgaagttcaacgcgccgcttgctcatctcatcatggccggagccgacgtgctcgccgtccccagccgcttcgagccctgtggactcatccagctgcaggggatgagatacggaacggtatacaatttccatctatcaattcgattgttcgatttcatctttgtgcaatgcaatgcaattgcaaatgcaaatgcatgatgattttccttgttgatttctccagccctgtgcttgcgcgtccaccggtgggctcgtggacacggtcatcgaaggcaagactggtttccacatgggccgtctcagcgtcgacgtaagcctatacatttacataacaatcagatatgacacatcctaataccgataagtcggtacactactacacatttacatggttgctggttatatggtttttttggcagtgcaaggtggtggagccaagcgacgtgaagaaggtggcggccaccctgaagcgcgccatcaaggtcgtcggcacgccggcgtacgaggagatggtcaggaactgcatgaaccaggacctctcctggaaggtataaattacgaaacaaatttaacccaaacatatactatatactccctccgcttctaaatattcaacgccgttgtcttttttaaatatgtttgaccattcgtcttattaaaaaaattaaataattataaattcttttcctatcatttgattcattgttaaatatacttatatgtatacatatagttttacatatttcataaaattttttgaacaagacgaacggtcaaacatgtgctaaaaagttaacggtgtcgaatattcagaaacggagggagtataaacgtcttgttcagaagttcagagattcacctgtctgatgctgatgatgattaattgtttgcaacatggatttcaggggcctgcgaagaactgggagaatgtgctcctgggcctgggcgtcgccggcagcgcgccggggatcgaaggcgacgagatcgcgccgctcgccaaggagaacgtggctgctccttgaagagcctgagatctacatatggagtgattaattaatatagcagtatatggatgagagacgaatgaaccagtggtttgtttgttgtagtgaatttgtagctatagccaattatataggctaataagtttgatgttgtactcttctgggtgtgcttaagtatcttatcggaccctg</dnaseqindica> 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