Difference between revisions of "Os01g0742500"
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===Mutation=== | ===Mutation=== | ||
| − | The expression of OsHXK5, OsHXK6, or their mutant alleles complemented the glucose insensitive2-1 mutant, thereby resulting in wild-type characteristics in seedling development, Glc-dependent gene expression, and plant growth. | + | The expression of OsHXK5, OsHXK6, or their mutant alleles complemented the glucose insensitive2-1 mutant, thereby resulting in wild-type characteristics in seedling development, Glc-dependent gene expression, and plant growth. |
| − | |||
===Orientation=== | ===Orientation=== | ||
Revision as of 16:29, 24 May 2014
Please input one-sentence summary here.
Contents
Annotated Information
Introduction
Hexokinase(HXK) plays a significant role in Glucose metabolism and Glucose signal transduction. Rice has ten HXK genes including OsHXK1~10[1][2]. The hexokinase 1 (AtHXK1) is recognized as an important glucose (Glc) sensor. It was also proved that OsHXK5 and OsHXK6 are evolutionarily related to AtHXK1.
Function
These OsHXKs including OsHXK6 retain a dual-targeting ability to mitochondria and nuclei. Some results also provide evidence that rice OsHXK5 and OsHXK6 can function as Glc sensors.
Expression
Transgenic rice plants overexpressing OsHXK5 or OsHXK6 exhibited hypersensitive plant growth retardation and enhanced repression of the photosynthetic gene RbcS in response to Glc treatment.
Mutation
The expression of OsHXK5, OsHXK6, or their mutant alleles complemented the glucose insensitive2-1 mutant, thereby resulting in wild-type characteristics in seedling development, Glc-dependent gene expression, and plant growth.
Orientation
Transient expression analyses using GFP fusion constructs revealed that OsHXK5 and OsHXK6 are associated with mitochondria.
Labs working on this gene
Please input related labs here.
References
Please input cited references here.
Structured Information
| Gene Name |
Os01g0742500 |
|---|---|
| Description |
Similar to Hexokinase |
| Version |
NM_001050749.1 GI:115439868 GeneID:4326547 |
| Length |
4329 bp |
| Definition |
Oryza sativa Japonica Group Os01g0742500, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 1:32765472..32769800 |
| Sequence Coding Region |
32765921..32766268,32766361..32766442,32766656..32766786,32766860..32766958,32767099..32767254 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008394:32765472..32769800 source=RiceChromosome01 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008394:32765472..32769800 source=RiceChromosome01 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggggaaggggacggtagtggggacggcggtggtggtgtgcgctgcagcggccgcggcggttggggtggcggtggtggtgtcgcggaggaggaggagcaagcgggaggcggaggaggagcggcggaggagggccgccgctgtgatcgaggaggtggagcagaggttctcgacgcccacggcgctgctgcgcggcatcgcggacgccatggtggaggagatggagcgcggcctccgcgccgaccctcacgccccgctcaagatgctcatcagctacgtcgacaacctccccaccggggatgagcacggactgttctatgctctcgatcttgggggcaccaatttccgtgttatacgtgttcagcttggaggaagggaaaagcgtgttgttagtcaacagtacgaagaggttgccattccacctcacctgatggttgggacttctatggaactgtttgacttcattgcggctgagttggaaagttttgtcaagaccgagggagaggatttccacttgccagagggcaggcagagagagttaggcttcaccttttctttcccagtgcaccaaacatcaatatcatcaggcactcttattaagtggacaaagggattttccatcaatggcacggtgggggaagatgttgtggctgaattgagcagggctatggaaaggcaagggcttgatatgaaagttacagctcttgttaatgacactgtaggcacattggctggcggaagatatgttgataatgacgttgctgctgctgtaatattaggcactggcacaaacgcagcctacgtggagcatgcaaatgcaattccaaaatggactggattactacctagatcaggaaatatggtgattaacatggaatggggaaacttcaagtcagaaaggcttcctcgttcagattacgataatgccttggactttgaaagtttaaacccaggcgagcagatatatgaaaagatgatttccggcatgtatcttggagagattgtgcgcagaatcttgcttaagcttgctcatgatgcttccttgtttggagatgttgttccaacaaagctggagcagcgctttatactgaggacgccggacatgtcagcgatgcatcatgatacctcacatgatctgaaacacctgggagctaagctgaaggatatcctgggggtcgctgatacttccctggaagcacgatacatcacccttcatgtctgcgacctcgttgcagagagaggtgcacgcttagctgctgctggtatatatggcattctaaagaagctgggcagggacagagtgccaagtgacggtagtcaaaagcagaggactgtcattgctctggatggtggtctctatgagcattacaagaagttcagaacctgcctagaagcaacgcttgcagacctgcttggagaggaggctgcctcatcagttgttgtcaagttggcaaacgatggctctggcatcggagctgcacttcttgcagcatctcactcccagtatgctagcgtcgagtag</cdnaseq> |
| Protein Sequence |
<aaseq>MGKGTVVGTAVVVCAAAAAAVGVAVVVSRRRRSKREAEEERRRR AAAVIEEVEQRFSTPTALLRGIADAMVEEMERGLRADPHAPLKMLISYVDNLPTGDEH GLFYALDLGGTNFRVIRVQLGGREKRVVSQQYEEVAIPPHLMVGTSMELFDFIAAELE SFVKTEGEDFHLPEGRQRELGFTFSFPVHQTSISSGTLIKWTKGFSINGTVGEDVVAE LSRAMERQGLDMKVTALVNDTVGTLAGGRYVDNDVAAAVILGTGTNAAYVEHANAIPK WTGLLPRSGNMVINMEWGNFKSERLPRSDYDNALDFESLNPGEQIYEKMISGMYLGEI VRRILLKLAHDASLFGDVVPTKLEQRFILRTPDMSAMHHDTSHDLKHLGAKLKDILGV ADTSLEARYITLHVCDLVAERGARLAAAGIYGILKKLGRDRVPSDGSQKQRTVIALDG GLYEHYKKFRTCLEATLADLLGEEAASSVVVKLANDGSGIGAALLAASHSQYASVE</aaseq> |
| Gene Sequence |
<dnaseqindica>3533..3880#3359..3440#3015..3145#2843..2941#2547..2702#2262..2336#1477..1659#1230..1380#186..481#agaggagagagactgagggagggaggcaggaggaaggaggaggaggaggacgctgcagtggtgggtggcgtagctcccgatccgggaagccgacccggtctgggggatttgctcctggcgcgcgctcgatcgagagggaggccagggttgggttggggggagcgtgaagaagcgcgcgcgcggctatggggaaggggacggtagtggggacggcggtggtggtgtgcgctgcagcggccgcggcggttggggtggcggtggtggtgtcgcggaggaggaggagcaagcgggaggcggaggaggagcggcggaggagggccgccgctgtgatcgaggaggtggagcagaggttctcgacgcccacggcgctgctgcgcggcatcgcggacgccatggtggaggagatggagcgcggcctccgcgccgaccctcacgccccgctcaagatgctcatcagctacgtcgacaacctccccaccgggtaggcctccctccgctccgcttgtgcgatttttatgatttctggggtgtacgtgacacgagaaagcttgggaatagttgttactgtgttcgcgaggggtgtttctcttcgggggcgaatcggttggaaatccctagcggcttagcgcttgcttaggttcggatttggcataaatgtggcattgatcttcggttgttgagatggaggtgcgacatattgatatcaagagcactgtgccttgacacggccttttagctcttttcttattgatcacggaagaatgatgagacctattcgtttcctatggaattttgatctactgagtggaggtttctcacggtggtagcagaaagtggccaccaggatagacttgacataatttttttttcgcagtgaagactccagacagaattataaaatgaacaattaggttccggtcacttttgacaactatatatatctgtgtgtccattttgtaatttgtgccttcatgctgggcttacgatcagtcgatcacagaacaatcgttcttttttttgtaccatgtgaattttgtaggaaatgtcatgaaaggtttatgaataagtttagagaaagaaatggtcaaggactcgaggcttaaaagtttcagtgagtttgacagtttattatatgtatccataggcgtatggaaaatgaaaaatgttgagcctatcacttcatgaaatcttcttttatgcttcctctgatttgcatgccacttgtgcagggatgagcacggactgttctatgctctcgatcttgggggcaccaatttccgtgttatacgtgttcagcttggaggaagggaaaagcgtgttgttagtcaacagtacgaagaggttgccattccacctcacctgatggttgggacttctatggtcagtggcttattcctttcaactgttatgtttcaattctcctgtgtttgcacaatttctttcatggttactcaaatttcagaaatatgagtacaggaactgtttgacttcattgcggctgagttggaaagttttgtcaagaccgagggagaggatttccacttgccagagggcaggcagagagagttaggcttcaccttttctttcccagtgcaccaaacatcaatatcatcaggcactcttattaagtggacaaagggattttccatcaatggcacggtaaaattcgaatggccttagtataaatttcatggctaaatgatctaggaattatttattaccttgacgatcagtcttgattagaaaccacgttgtgtcagcaaacgaattctcactagctgtgtcacttttgacttttgggttccctgcattgaccaagtgaccagccgttgcccttcattttcctttcagaatatttggactgatgtttggttatccataaattaacatgtttggtaaagttattcttagatgtgatataaacgggacatggtggcatatgtccccatttatccaattcacaagtatcttataattgcttctaaatcctgcggcgattgtgaatgcagatgttacaattagagctgtgtttgctatacaacacttgtaaccataaaaaaatgtatttgcttatcctgcacttcttaaatttgaacctccaagaatgaagcactctagtcatatttctgtttcctacttttaatgtggcaatttctagcctctcaagacaaatgtggcatcaacactgcaatatcttttttaattttataatgttatgcccataattttgatgtatagactaatgatttctgtgaactcaggtgggggaagatgttgtggctgaattgagcagggctatggaaaggcaagggcttgatatgaaagttacagctcttgtaagtaaattcttggttcatgactctgttaaaattatttatcatctgaaactttcccatgcctgctactgctatatgctgatgggtaaatacattggcattcctatgaaaatgctgactttggagtttttcaatgttctatgagtttggatgtttactagtatatcaccagactgtgtcatgtaaattgacaactaatattttgcccaggttaatgacactgtaggcacattggctggcggaagatatgttgataatgacgttgctgctgctgtaatattaggcactggcacaaacgcagcctacgtggagcatgcaaatgcaattccaaaatggactggattactacctagatcaggaaatatggttagtgactgatttcgttatacttgtagcattaaatgtctttgaaccttgtataaatgtatgtatccacttcaacttctgcactggatgattgtcacatcaattaatattataacatttggatctttggtatgatgaaggtgattaacatggaatggggaaacttcaagtcagaaaggcttcctcgttcagattacgataatgccttggactttgaaagtttaaacccaggcgagcaggtattgtttattttatctttttattttccaactatatgtgggcatgaaagaatttcggtcttcttttcctcagatatatgaaaagatgatttccggcatgtatcttggagagattgtgcgcagaatcttgcttaagcttgctcatgatgcttccttgtttggagatgttgttccaacaaagctggagcagcgctttatactgaggtgcacttctatgtcccctatgttgatcatctaagtatctagcttttcatatatgcagcacagaactatcaaaataagataaaatttcaacatagactgtatggtgtgcattatgcgttccttgatcatggtagcacagaactatcaaaataagataaattcatgtaactatgtagaaaatttcttaacggaactccaattgtggtattgcaggacgccggacatgtcagcgatgcatcatgatacctcacatgatctgaaacacctgggagctaagctgaaggatatcctgggggtatgataatacatatcttgaaaataggatactatcaaatctaattttcaagaaccattgattacttacgatatgcctgaacttatgtttaggtcgctgatacttccctggaagcacgatacatcacccttcatgtctgcgacctcgttgcagagagaggtgcacgcttagctgctgctggtatatatggcattctaaagaagctgggcagggacagagtgccaagtgacggtagtcaaaagcagaggactgtcattgctctggatggtggtctctatgagcattacaagaagttcagaacctgcctagaagcaacgcttgcagacctgcttggagaggaggctgcctcatcagttgttgtcaagttggcaaacgatggctctggcatcggagctgcacttcttgcagcatctcactcccagtatgctagcgtcgagtagtaacaggagctcatgggactgagctcccagtgtagcttgttttcctcccattttccccgtttctttccaatgggagttcgtttccctcctgcgattcgcatctccttttgctattctgcagtcacataaacgagtgcctgtgcagcgggatgtagctagtatggcgccaaagagtttgcagttatcacatgaacaagcatttgcaactgcagggaagtgaaaacgggggcttgaatgatgccgttcttttcctgcaaattattttccccctttccctgtaagtttgtattgtgatgcgatgtcgcaaaccaatcacagcggtttcgcgtgtagccttttgtcattcagatttcagaataaagagggggacataatttcacttttcgtatgtcagtttgaactatgtatgtactcttacacactggtatcaaagctcaaatgatctgatc</dnaseqindica> |
| External Link(s) |
