Difference between revisions of "Os09g0561600"
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==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
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| + | OsDEES1,a rice WAK-RLK gene,plays a role in rice sexual reproduction by regulating female gametophyte development.OsDEES1 silencing by RNA interference caused a high rate of female sterility. Crossing experiments showed that female reproductive organs lacking OsDEES1 carried a functional defect. | ||
| + | ===The phenotype of OsDEES1 RNA interference plants=== | ||
| + | the knockdown of OsDEES1 expression did not affect megasporogenesis | ||
| + | but that it disturbed female gametophyte formation, resulting in a degenerated embryo sac and defective seed formation.The RNAi transgenic | ||
| + | rice exhibited a near-normal morphology in terms of growth but a high rate of sterility (Fig. 1, A and B).Of 44 RNAi transgenic plants obtained over | ||
| + | three years (2005, 2006, and 2007), 33 had high rates of sterility (Fig. 1C).A semiquantitative reverse transcription (RT)-PCR assay revealed that the mRNA expression of endogenous OsDEES1 was significantly | ||
| + | down-regulated in our RNAi plants (Fig. 1D). | ||
| + | [[File:Fig 1.png|right|thumb|320px|'''Figure 1. Analysis of the sterile phenotype in the OsDEES1 RNAi transgenic rice plants.''' ]] | ||
===Expression=== | ===Expression=== | ||
Revision as of 09:10, 25 May 2014
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Contents
Annotated Information
Function
OsDEES1,a rice WAK-RLK gene,plays a role in rice sexual reproduction by regulating female gametophyte development.OsDEES1 silencing by RNA interference caused a high rate of female sterility. Crossing experiments showed that female reproductive organs lacking OsDEES1 carried a functional defect.
The phenotype of OsDEES1 RNA interference plants
the knockdown of OsDEES1 expression did not affect megasporogenesis but that it disturbed female gametophyte formation, resulting in a degenerated embryo sac and defective seed formation.The RNAi transgenic rice exhibited a near-normal morphology in terms of growth but a high rate of sterility (Fig. 1, A and B).Of 44 RNAi transgenic plants obtained over three years (2005, 2006, and 2007), 33 had high rates of sterility (Fig. 1C).A semiquantitative reverse transcription (RT)-PCR assay revealed that the mRNA expression of endogenous OsDEES1 was significantly down-regulated in our RNAi plants (Fig. 1D).
Expression
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Evolution
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Labs working on this gene
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References
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Structured Information
| Gene Name |
Os09g0561600 |
|---|---|
| Description |
EGF domain containing protein |
| Version |
NM_001070468.1 GI:115480678 GeneID:4347857 |
| Length |
2988 bp |
| Definition |
Oryza sativa Japonica Group Os09g0561600, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 9:23147405..23150392 |
| Sequence Coding Region |
23147423..23148569,23149076..23150091 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008402:23147405..23150392 source=RiceChromosome09 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008402:23147405..23150392 source=RiceChromosome09 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggcgatggcgatggcgatggcgctgctgctgctgctgcttctgcagctgtggtcggtggaagcgcaggtcgcagcgccgccgccggcgagctgcccggacaggtgcggcgacgtgagcgtgccgtacccgttcggcatccgcgacggctgccacctcccgggcttccgcctcacctgcgacgccacccacaccccgccgcgcctgatgctcggcaacggcaccctccaggtcgtcgacatctccctggccaactccaccgtgcgcgccctcgacctcgccggcgccgtcaacttcacctacgacgtgtcgaagctcgcccccagcggcagcggcacgtggtccagcctcggcaccgtcgccggcgccggcccgtacgtcgtctccgagcagcgcaaccggctcgtcgtcacgggctgcaacgtccaggccacgctcgccggggagaacaccaacatcatcggcggctgctcctccttctgcccggtcagcgagatgttcaccagcgtggcggcgaccgtccccgtcgtccccggcgccggcgccgacaacgccaccgatggcggcttcatctgctccggcaccagctgctgcgagacgcccatcgccatcggccgcccctcctacctcgtgcagttcctcagccttgaccagaaccaggagctcaccggcaagctccccgtcgccgtgcgcatcgccgagcgtgggtggttcgagggcgtcgccggcgagctgctcaacaccagctccgactccgccgccgccctccggacgccggtcccggtggtgctggagtgggtggtgtcgccgacgctggaggcggtgctgcagggcgtcaccgggcagttcgccgacgaccgcaactggtcgtgccccgcggacgcggcgaggagcgcatgccggagcagcaacagcttctgcagcaacgtcaccggcaactaccgccgcggctacgtgtgcaggtgccggcgaggctacggcgggaacccctacgtcgccggcggatgccaagacatcgacgagtgcaagctcgccgggaggtgctacggcgagtgcaccaacacgccgggagattaccagtgccggtgcccgcgcggcgctcgtggcgacccgcgcattccaaacggctgcgtcaaaactaatctaggtttaagtgttggtattggagttggcagtggagctgggcttttggtaatgggactgggcgctgcctttttaaaacgtaaggttaagaaacagagagcaagaatgctgaggcagaagttcttcaagcagaaccgaggtcatttgttgcaacaactagtgtctcagaaggctgacattgctgaaaggatgatcatccccttgtcagaactggagaaggccacaaacaattttgataaatcacgtgagcttggaggaggggggcacggcaccgtgtacaaagggattttatcggatcttcatgttgtcgcgatcaagaaatctaaggaggcagttcaaagagagattgacgagttcataaatgaggtagccattctctcgcaaatcaaccatcgtaatgtggtcaaactttttggatgttgtcttgagacagaagtgccattgctggtttatgaattcatatcaaatggtaccctttaccaccatcttcatgttgaaggaccaatgtcattgccatgggaagataggctcagaattgcaaccgaaaccgctagagcacttgcctaccttcactcagctgtgtcgttccctataatccacagggatatcaaatcccataacatcctattagatggttcactaacaacaaaagtgtccaattttggagcttcaaggtgcattccagctgaacaaactgggataacaaccgttgttcaaggaacactaggatatctggaccccatgtactactacacagggcgtctcactgagaaaagcgatgttttcagcttcggcgtcgttctaatagagttgcttactaggaagaaaccatactcatacagatcgcctgatgatgaaagtcttgtaacacatttcactgccctactcacacaaggcaacttgggcgacatacttgatcctcaggtcaaggaagagggaggcgaagaagttaaagagatagctgtgttagctgtggcatgtgccaagctgaaagtagaggagcggcctactatgaggtag</cdnaseq> |
| Protein Sequence |
<aaseq>MAMAMAMALLLLLLLQLWSVEAQVAAPPPASCPDRCGDVSVPYP FGIRDGCHLPGFRLTCDATHTPPRLMLGNGTLQVVDISLANSTVRALDLAGAVNFTYD VSKLAPSGSGTWSSLGTVAGAGPYVVSEQRNRLVVTGCNVQATLAGENTNIIGGCSSF CPVSEMFTSVAATVPVVPGAGADNATDGGFICSGTSCCETPIAIGRPSYLVQFLSLDQ NQELTGKLPVAVRIAERGWFEGVAGELLNTSSDSAAALRTPVPVVLEWVVSPTLEAVL QGVTGQFADDRNWSCPADAARSACRSSNSFCSNVTGNYRRGYVCRCRRGYGGNPYVAG GCQDIDECKLAGRCYGECTNTPGDYQCRCPRGARGDPRIPNGCVKTNLGLSVGIGVGS GAGLLVMGLGAAFLKRKVKKQRARMLRQKFFKQNRGHLLQQLVSQKADIAERMIIPLS ELEKATNNFDKSRELGGGGHGTVYKGILSDLHVVAIKKSKEAVQREIDEFINEVAILS QINHRNVVKLFGCCLETEVPLLVYEFISNGTLYHHLHVEGPMSLPWEDRLRIATETAR ALAYLHSAVSFPIIHRDIKSHNILLDGSLTTKVSNFGASRCIPAEQTGITTVVQGTLG YLDPMYYYTGRLTEKSDVFSFGVVLIELLTRKKPYSYRSPDDESLVTHFTALLTQGNL GDILDPQVKEEGGEEVKEIAVLAVACAKLKVEERPTMR</aaseq> |
| Gene Sequence |
<dnaseqindica>19..1165#1672..2687#atcattcaaccaccggctatggcgatggcgatggcgatggcgctgctgctgctgctgcttctgcagctgtggtcggtggaagcgcaggtcgcagcgccgccgccggcgagctgcccggacaggtgcggcgacgtgagcgtgccgtacccgttcggcatccgcgacggctgccacctcccgggcttccgcctcacctgcgacgccacccacaccccgccgcgcctgatgctcggcaacggcaccctccaggtcgtcgacatctccctggccaactccaccgtgcgcgccctcgacctcgccggcgccgtcaacttcacctacgacgtgtcgaagctcgcccccagcggcagcggcacgtggtccagcctcggcaccgtcgccggcgccggcccgtacgtcgtctccgagcagcgcaaccggctcgtcgtcacgggctgcaacgtccaggccacgctcgccggggagaacaccaacatcatcggcggctgctcctccttctgcccggtcagcgagatgttcaccagcgtggcggcgaccgtccccgtcgtccccggcgccggcgccgacaacgccaccgatggcggcttcatctgctccggcaccagctgctgcgagacgcccatcgccatcggccgcccctcctacctcgtgcagttcctcagccttgaccagaaccaggagctcaccggcaagctccccgtcgccgtgcgcatcgccgagcgtgggtggttcgagggcgtcgccggcgagctgctcaacaccagctccgactccgccgccgccctccggacgccggtcccggtggtgctggagtgggtggtgtcgccgacgctggaggcggtgctgcagggcgtcaccgggcagttcgccgacgaccgcaactggtcgtgccccgcggacgcggcgaggagcgcatgccggagcagcaacagcttctgcagcaacgtcaccggcaactaccgccgcggctacgtgtgcaggtgccggcgaggctacggcgggaacccctacgtcgccggcggatgccaagacatcgacgagtgcaagctcgccgggaggtgctacggcgagtgcaccaacacgccgggagattaccagtgccggtgcccgcgcggcgctcgtggcgacccgcgcattccaaacggctgcgtcaaaactaatctaggtaactacacctacatttttctgctttgacatagtccaatttatgcacagatttttcaaagaatggaaaactaatctaggtactaataactgaaatatcctgctttacattgtcagtcaaatttatgcagagagctctgcaatgtagttctgttttatacagcagtctttctttaactgttcttccaagtacttgcatcgactactctgttttcatagactgatcttgcttttcttattaaccaaatggctgataaattattttacttgatagtgctagctctgcaatgaaactctgtttagtacatcagtctttctttaacagttcagtcaagtactagcaccaactgctctgtttcataaacacaagcatactactctgttttttcatagactaatctttccataacgttgtttttcttattaaccaactcccatattctagttcataggtgaaatatctaccaactctgatggagatttttgccttctcttgtgtgctaccaggtttaagtgttggtattggagttggcagtggagctgggcttttggtaatgggactgggcgctgcctttttaaaacgtaaggttaagaaacagagagcaagaatgctgaggcagaagttcttcaagcagaaccgaggtcatttgttgcaacaactagtgtctcagaaggctgacattgctgaaaggatgatcatccccttgtcagaactggagaaggccacaaacaattttgataaatcacgtgagcttggaggaggggggcacggcaccgtgtacaaagggattttatcggatcttcatgttgtcgcgatcaagaaatctaaggaggcagttcaaagagagattgacgagttcataaatgaggtagccattctctcgcaaatcaaccatcgtaatgtggtcaaactttttggatgttgtcttgagacagaagtgccattgctggtttatgaattcatatcaaatggtaccctttaccaccatcttcatgttgaaggaccaatgtcattgccatgggaagataggctcagaattgcaaccgaaaccgctagagcacttgcctaccttcactcagctgtgtcgttccctataatccacagggatatcaaatcccataacatcctattagatggttcactaacaacaaaagtgtccaattttggagcttcaaggtgcattccagctgaacaaactgggataacaaccgttgttcaaggaacactaggatatctggaccccatgtactactacacagggcgtctcactgagaaaagcgatgttttcagcttcggcgtcgttctaatagagttgcttactaggaagaaaccatactcatacagatcgcctgatgatgaaagtcttgtaacacatttcactgccctactcacacaaggcaacttgggcgacatacttgatcctcaggtcaaggaagagggaggcgaagaagttaaagagatagctgtgttagctgtggcatgtgccaagctgaaagtagaggagcggcctactatgaggtaggtggagatgacactcgaaagtgttcgattgtcatcacagcagcaagagcatggtgtgggtgcaaagaaatccagggagaatcatgtctcctggagctacccggtaagtgagggtacaagtacacaatcaactagacaatatagccttgaagaagagtatttgttgtcatcaaggtttcccagataatggatttttttttctggagtggttttggatcaatagaaagagttgtcacaatgtacaaggcatatgatatgcatgattttcatagctaaatgtgcactgttttattgttcttctt</dnaseqindica> |
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