Difference between revisions of "Os08g0531600"
| Line 2: | Line 2: | ||
==Annotated Information== | ==Annotated Information== | ||
| + | qGW8 within a genetic window on the long arm of chromosome 8 defined by the markers RM80 and RM447.The progeny testing of homozygous recombinant plants allowed this region to be narrowed to an ~7.5-kb stretch flanked by RM502 and PSM711.Quantitative trait locus GW8 is synonymous with OsSPL16, which encodes a protein that is a positive regulator of cell proliferation.Higher expression of this gene promotes cell division and grain filling, with positive consequences for grain width and yield in rice. Conversely, a loss-of-function mutation in Basmati rice is associated with the formation of a more slender grain and better quality of appearance. The correlation between grain size and allelic variation at the GW8 locus suggests that mutations within the promoter region were likely selected in rice breeding programs. We also show that a marker-assisted strategy targeted at elite alleles of GS3 and OsSPL16 underlying grain size and shape can be effectively used to simultaneously improve grain quality and yield. | ||
===Function=== | ===Function=== | ||
[[File:Parental grains.jpg|frame|'''Figure1''' Parental grains. Scale bar, 3 mm.]] | [[File:Parental grains.jpg|frame|'''Figure1''' Parental grains. Scale bar, 3 mm.]] | ||
'''''OsSPL16''''' control of grain size, shape, and quality<ref name="ref1" />. | '''''OsSPL16''''' control of grain size, shape, and quality<ref name="ref1" />. | ||
| − | '''''OsSPL16''''' encodes a protein that is a positive regulator of cell proliferation. Higher expression of this gene promotes cell division and grain filling, with positive consequences for grain width and yield in rice. The candidate gene ''''''OsSPL16'''''' encodes squamosa promoter-binding protein-like 16, which belongs to the SBP domain family of transcription factors and shares homology with the product of '''''tga1''''',a domestication syndrome gene associated with the formation of grains in maize<ref name="ref1" />. Grain width and length were also altered in NIL-'''''qw8''''' plaints expressing the Basmati385 '''''OsSPL16''''' cDNA under the control of the native HJX74 promoter. | + | '''''OsSPL16''''' encodes a protein that is a positive regulator of cell proliferation. OsSPL16contributes to organ size through its effect on the cell cycle machinery.Higher expression of this gene promotes cell division and grain filling, with positive consequences for grain width and yield in rice. The candidate gene ''''''OsSPL16'''''' encodes squamosa promoter-binding protein-like 16, which belongs to the SBP domain family of transcription factors and shares homology with the product of '''''tga1''''',a domestication syndrome gene associated with the formation of grains in maize<ref name="ref1" />. Grain width and length were also altered in NIL-'''''qw8''''' plaints expressing the Basmati385 '''''OsSPL16''''' cDNA under the control of the native HJX74 promoter.The SPL genes have an important role in the control of flowering.The SPL genes have been shown to be regulated by microRNA miR156 (refs. 14–16,19–22), and OsSPL16contains an OsmiR156 target sequence. OsSPL16functions as a negative regulator of panicle branching. |
| Line 20: | Line 21: | ||
===Mutation=== | ===Mutation=== | ||
| − | + | Higher expression of this gene promotes cell division and grain filling, with positive consequences for grain width and yield in rice.A loss-of-function mutation in Basmati rice is assciated with the formation of a more slender grain and better quality of apperance. The correlation between grain size and allelic variation at the GW8 locus suggests that mutations within the promoter region were likely selected in rice breeding programs<ref name="ref1" />. | |
| − | A loss-of-function mutation in Basmati rice is assciated with the formation of a more slender grain and better quality of apperance. The correlation between grain size and allelic variation at the GW8 locus suggests that mutations within the promoter region were likely selected in rice breeding programs<ref name="ref1" />. | ||
| Line 50: | Line 50: | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, National | |
| + | Centre for Plant Gene Research, Beijing, China. | ||
| + | The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural | ||
| + | University, Guangzhou, China. | ||
| + | The State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China. | ||
| + | working | ||
| + | Development of the SSSL population.Fine mapping of qGW8.Transgene constructs.Expression analysis.Histological analysis.Transactivation activity assay.Analysis of transgenic plants expressing OsSPL16. | ||
==References== | ==References== | ||
Revision as of 15:11, 27 May 2014
Os08g0531600(OsSPL16) is a very important gene that encoding a positive regulator of cell prolifertion which can pormote cell division and grain filling.
Contents
Annotated Information
qGW8 within a genetic window on the long arm of chromosome 8 defined by the markers RM80 and RM447.The progeny testing of homozygous recombinant plants allowed this region to be narrowed to an ~7.5-kb stretch flanked by RM502 and PSM711.Quantitative trait locus GW8 is synonymous with OsSPL16, which encodes a protein that is a positive regulator of cell proliferation.Higher expression of this gene promotes cell division and grain filling, with positive consequences for grain width and yield in rice. Conversely, a loss-of-function mutation in Basmati rice is associated with the formation of a more slender grain and better quality of appearance. The correlation between grain size and allelic variation at the GW8 locus suggests that mutations within the promoter region were likely selected in rice breeding programs. We also show that a marker-assisted strategy targeted at elite alleles of GS3 and OsSPL16 underlying grain size and shape can be effectively used to simultaneously improve grain quality and yield.
Function
OsSPL16 control of grain size, shape, and quality[1]. OsSPL16 encodes a protein that is a positive regulator of cell proliferation. OsSPL16contributes to organ size through its effect on the cell cycle machinery.Higher expression of this gene promotes cell division and grain filling, with positive consequences for grain width and yield in rice. The candidate gene 'OsSPL16' encodes squamosa promoter-binding protein-like 16, which belongs to the SBP domain family of transcription factors and shares homology with the product of tga1,a domestication syndrome gene associated with the formation of grains in maize[1]. Grain width and length were also altered in NIL-qw8 plaints expressing the Basmati385 OsSPL16 cDNA under the control of the native HJX74 promoter.The SPL genes have an important role in the control of flowering.The SPL genes have been shown to be regulated by microRNA miR156 (refs. 14–16,19–22), and OsSPL16contains an OsmiR156 target sequence. OsSPL16functions as a negative regulator of panicle branching.
Mutation
Higher expression of this gene promotes cell division and grain filling, with positive consequences for grain width and yield in rice.A loss-of-function mutation in Basmati rice is assciated with the formation of a more slender grain and better quality of apperance. The correlation between grain size and allelic variation at the GW8 locus suggests that mutations within the promoter region were likely selected in rice breeding programs[1].
Expression
The expression profiles of OsSPL16in various organs of HJX74 were examined by quantitative RT-PCR analysis. OsSPL16 was preferentially expressed in developing panicles, and the highest levels of OsSPL16expression were found in panicles of 7 cm in length, whereas there was less transcript accumulation in the root, culm, leaf sheath, shoot meristem and young panicle(of <1 cm in length)[1]
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this gene
The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, National Centre for Plant Gene Research, Beijing, China. The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China. The State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China. working Development of the SSSL population.Fine mapping of qGW8.Transgene constructs.Expression analysis.Histological analysis.Transactivation activity assay.Analysis of transgenic plants expressing OsSPL16.
References
<references>
Structured Information
| Gene Name |
Os08g0531600 |
|---|---|
| Description |
Similar to Squamosa-promoter binding-like protein 3 |
| Version |
NM_001068869.1 GI:115477476 GeneID:4346133 |
| Length |
5032 bp |
| Definition |
Oryza sativa Japonica Group Os08g0531600, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 8:26589172..26594203 |
| Sequence Coding Region |
26589228..26589718,26592621..26592751,26593194..26593939 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008401:26589172..26594203 source=RiceChromosome08 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008401:26589172..26594203 source=RiceChromosome08 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggagtgggatctcaagatgccgccggcggcgagctgggagctagccgacgagctggagaacagcggcggcgggggtgtaccggcggcggtatcgtcgtcatcggctgcggttggtggcggcgtcaatgcggggggtggtggcaggcaggagtgctcggtcgacctcaagctcggcgggttgggggagttcggcggcggcggcgcgcagccgcgggtcgccgtggcgggcgagccggccaaggggaaggggccagcggccgccgccacgggagcagcagcagcagcgtcgtcggcgccggcgaagcggccgcgcggtgcggcggcggcggggcagcagcagtgcccgtcgtgcgcggtggacgggtgcaaggaggacctgagcaagtgccgcgactaccatcgccggcacaaggtgtgcgaggcccactccaagacccccctcgtcgtcgtctccggccgcgagatgcgcttctgccagcagtgcagcaggtttcacttgcttcaggagtttgatgaggccaagcgcagctgtagaaagcgactagatgggcacaaccgtcgccgcaggaagccacagccagatcccatgaactctgcaagttatcttgcaagccaacaaggggcaagattctcaccgttcgcgacgccgagaccggaggcaagctggacagggatgatcaaaaccgaggagagcccatactacacgcaccaccaaatccctcttggcatcagcagcaggcagcagcatttcgttggctccacctctgacggcggccgccgcttccctttcctccaggaaggcgagatcagcttcggcaccggcgccggcgccggcggcgtgccaatggatcaggcagcagctgctgctgctgcttcagtgtgccagccacttctgaagacggtagctcctcctcctcctcctcatggcggcggcggcagcggcggcggcaagatgttctccgatggtgggttgacacaagtgctcgactccgattgtgctctctctcttctgtcagctccggcgaactccacggccatcgacgtcggcggtggccgggtggtcgtccagccgaccgagcacatccccatggcgcagcctctcatctctggccttcagttcggcggcggcggcggcagctcagcctggttcgcggcgcggccgcatcatcaggcggccaccggcgccgccgccaccgccgtcgtcgtctcgacggccggtttctcctgcccggtggtggagagcgagcagctgaacacagtcctgagctccaatgacaatgagatgaactacaatgggatgtttcacgtcggcggcgaaggctcatcggatggcacgtcgtcgtctctgccgttctcatggcagtag</cdnaseq> |
| Protein Sequence |
<aaseq>MEWDLKMPPAASWELADELENSGGGGVPAAVSSSSAAVGGGVNA GGGGRQECSVDLKLGGLGEFGGGGAQPRVAVAGEPAKGKGPAAAATGAAAAASSAPAK RPRGAAAAGQQQCPSCAVDGCKEDLSKCRDYHRRHKVCEAHSKTPLVVVSGREMRFCQ QCSRFHLLQEFDEAKRSCRKRLDGHNRRRRKPQPDPMNSASYLASQQGARFSPFATPR PEASWTGMIKTEESPYYTHHQIPLGISSRQQHFVGSTSDGGRRFPFLQEGEISFGTGA GAGGVPMDQAAAAAAASVCQPLLKTVAPPPPPHGGGGSGGGKMFSDGGLTQVLDSDCA LSLLSAPANSTAIDVGGGRVVVQPTEHIPMAQPLISGLQFGGGGGSSAWFAARPHHQA ATGAAATAVVVSTAGFSCPVVESEQLNTVLSSNDNEMNYNGMFHVGGEGSSDGTSSSL PFSWQ</aaseq> |
| Gene Sequence |
<dnaseqindica>57..547#3450..3580#4023..4768#acagctcaagcttacgcgggagctaagctgagctacagcgagcggcggcggcggccatggagtgggatctcaagatgccgccggcggcgagctgggagctagccgacgagctggagaacagcggcggcgggggtgtaccggcggcggtatcgtcgtcatcggctgcggttggtggcggcgtcaatgcggggggtggtggcaggcaggagtgctcggtcgacctcaagctcggcgggttgggggagttcggcggcggcggcgcgcagccgcgggtcgccgtggcgggcgagccggccaaggggaaggggccagcggccgccgccacgggagcagcagcagcagcgtcgtcggcgccggcgaagcggccgcgcggtgcggcggcggcggggcagcagcagtgcccgtcgtgcgcggtggacgggtgcaaggaggacctgagcaagtgccgcgactaccatcgccggcacaaggtgtgcgaggcccactccaagacccccctcgtcgtcgtctccggccgcgagatgcgcttctgccagcagtgcagcaggtaaccccccccccccccccccaaccattgtctccttccttcccgccaaattcactgcaaaacaaaaaaaaaatcgtagcccaaaacaccccaagacgtcatggcaattcgcatcaagaactgcatatatcaatttctccacttcttttcagcgtcactgtctctgatcattctctttgctgaacaaaagaaaaagaagataagcaagagtttttctcttttttttgctccttttttttttggctttgcacaatctcttcttgcttccagttgcaactgaccattgtgcagtacatgcatctgcatctactgattctaatttctacgctacttcggatcaaaattaattcagtactgcaaagcacaatttcattgatccatttcatccagcctcggactttgttcatcatcatctatctgtctcttacttcctttccattgggagcatactatccggctgtctcgtttcagggacgcacagctttgcctttaatggcatgccttttcagccttccctcatgctatcctttagctcggcaactcgtattaccccaaattattacctctttgctcgcctttagatttattactatcatcttttcttttcttttttatatctcttcttcaccagtagctgcactgtttttgcactgctcaagagcaaagcagctgctgtagttgttcagtgtttgttgcttactgagaaaaaaaagtgatagagacagaaaaaaaagtgagggagagaaaaaaaaaaaaacagaactgacgcctgaatctcatcagccagagatcacattaggcaatttaccaccagactgttatgatattattttcagtgtcctcctgtctgaatatgaccgtctgcttcctctaacaagaacaataaatcagcacctagttcagtactaactaattttctcatgaataaataaataaatatagtcactgtaattagtgacactactagcacggtagcacctggtttagtggttaacaatacttggttcttgcacttctccctgtcgatgttttttcgcgtgggggctagctatcgattgattgattcctcaactatggcatcgaaactggaagaacatatgcatactgggacacacaccctgcttgctttctgaatttctgatttctcctcaaggcagctggcctaccacatatatctgactgagctgtgctgcttcttgccatgagagctaagctaccttagcttagctactactaccacttactacgccgtctgttttggaagggaaaggcagatgtggatgcccaaacctagaaagatggttgtaccactgaaagagagagtttgtggatgtgatctgcactaaagcacccctgtacagggaaaggaccatgtagccctactacaagttcaccatttacacctctgttcctaaggttgggccacacatatatgaagcttttaatgtctcggtttgttggaaagggttttgcattgccattacaagccagcacagtggatacagatagccagggtgctctctattggagaagaaaaaaaatggagccctgaacaccctgattggatctcactattgcatgaaagaatgatgagatttcttgtcttataatttttaaagattttttttctaaagtcagtcttagttacattcatttgttatattccagtttcagacttattggtactaggttctgtgagatctttttttttttttacatcgtttgagtatcatagggtgattcagtaccaccttgacccctgtttttatcagagctctaaacttctaacaccacttctaacttttgagctagtcttctaaccttgctgttttctgaacaaagatgtatactcaagattggtcatagatggagatattctgtgaacagaactaacataatagcaccaaattagtcagacatactctttacaaaattactttggagtttgttgtccactccttgaactagtacaatattgtcctactgaatgccttcctgcctttcaacttgaaagttccctattttatctgttagttcttttataaaatgtaactgcacattgtcagaaggatttgcatcttatttcactttgcgccagttttaagtaatacatggtatattggcataagaccagactctaccattttttatcttgcagagacatagcaaacaactaagtactttttattgtggtgtgctcctttacacagtagcacaacttgtaggatgcttatgtgattgtctcatcaattattctctttatctttaaaaagagaatgatacaaaaaatctctttatctgagaatacacattacccagtggggacagtctttcaatgatttgattacttcgtcagtgtttgcaaactgggaagatcattatgctgctgcatgcagactttataaattaagtgatcttcagagtcagaacaagatgttagctttctatacctatggatccacatccactgtattgtggtccatgtacaagtggggttaaaatatttttctgccgttgacagaacttcagttcaataaatttatctaagatgaagtatccaagcacggaaagagctaattaactgatgaaattcctgtggtcccttgtgttggtatatgagtattctaagagagaatatggagacagtatattaaattattctgagaatacttatcctgacgtttctttagtgagaactgtggtgcatcgttacaaaacttcagatcatgtttcaggagtattttatcatgtaagaattttaaaaagacgtacatcctaggtacagtcatttcttaaggtttcatggtactgaatgattaaattacttcttctggattgggtttcaagcatcatttggctaatttcaatgcagttaaatgatcataagcttttctttcttcaggtttcacttgcttcaggagtttgatgaggccaagcgcagctgtagaaagcgactagatgggcacaaccgtcgccgcaggaagccacagccagatcccatgaactctgcaagttatcttgcaagccaacaaggtattttcttgtttattattaccactctatgatatcgcagttcatataagattaactgggatatagtcattcagacttcctaactattgttagactaggaaaaaaactatgaaacatgctaatagcatagataagtcatggtaaaaaaaaagtaaaagaaaatgaaactgtggttaaaaaaaaacgcaaatattagggaatgacctaatatcaaataattagaaggagtgaggcttcgaacccaggtcgtctagcccatcaccttttgaagctagccagaaaacccctgggcgtttctcagaactgtggttcagctatgactctgttctttcaatcctgacatcttgtaacatgtaatgcattctagtatacatctaatgcattgaaccatatcttatgtactaatttgtgctgatatatcaaacatcgcatcaaaattcaggggcaagattctcaccgttcgcgacgccgagaccggaggcaagctggacagggatgatcaaaaccgaggagagcccatactacacgcaccaccaaatccctcttggcatcagcagcaggcagcagcatttcgttggctccacctctgacggcggccgccgcttccctttcctccaggaaggcgagatcagcttcggcaccggcgccggcgccggcggcgtgccaatggatcaggcagcagctgctgctgctgcttcagtgtgccagccacttctgaagacggtagctcctcctcctcctcctcatggcggcggcggcagcggcggcggcaagatgttctccgatggtgggttgacacaagtgctcgactccgattgtgctctctctcttctgtcagctccggcgaactccacggccatcgacgtcggcggtggccgggtggtcgtccagccgaccgagcacatccccatggcgcagcctctcatctctggccttcagttcggcggcggcggcggcagctcagcctggttcgcggcgcggccgcatcatcaggcggccaccggcgccgccgccaccgccgtcgtcgtctcgacggccggtttctcctgcccggtggtggagagcgagcagctgaacacagtcctgagctccaatgacaatgagatgaactacaatgggatgtttcacgtcggcggcgaaggctcatcggatggcacgtcgtcgtctctgccgttctcatggcagtagttttttcagtaactgtatgttgctgccttagtttcagtagagttggttcttcatttcttttcagtgatcaaattattgtttctgttcttttctgccatggtaagttccttttttttttcttcttcttgccttcatttgagttaattacagcattgatttgtgtgaacaaaattcatcataaatcagttcctcgcgagatcattggtctcaacatgatggtgccaagtgagaactgcagtattgtgcagttttcagttttgagtc</dnaseqindica> |
| External Link(s) |
Gene Attributes Chromosome: Chr8 CDS Coordinates (5'-3'): 26501167 - 26506218 Nucleotide length: 1368 Predicted protein length: 456 Predicted molecular weight: 46578.9 Predicted pI: 7.502
Gene Structure Gene Attributes Chromosome: Chr8 CDS Coordinates (5'-3'): 26501167 - 26506218 Nucleotide length: 1368 Predicted protein length: 456 Predicted molecular weight: 46578.9 Predicted pI: 7.502
Gene Structure
