AY986492

Function

Resistant and susceptible alleles of Xa27 encode identical proteins. However, expression of only the resistant allele occurs when a rice plant is challenged by bacteria harbouring avrXa27, whose product is a nuclear localized type-III effector. Induction of Xa27 occurs only in the immediate vicinity of infected tissue, whereas ectopic expression of Xa27 resulted in resistance to otherwise compatible strains of the pathogen. Thus Xa27 specificity towards incompatible pathogens involves the differential expression of the R gene in the presence of the AvrXa27 effector.

Expression

The Xa27(t) locus conferred a high level of resistance to 27 strains and moderate resistance to three strains. Resistance of the Xa27(t) gene was developmentally regulated in IRBB27 and showed semidominant or a dosage effect in the cv. CO39 genetic background. As a prelude to cloning Xa27(t), a molecular mapping strategy was employed with a large mapping population consisting of 3,875 gametes

Evolution

Induction of Xa27 occurs only in the immediate vicinity of infected tissue, whereas ectopic expression of Xa27 resulted in resistance to otherwise compatible strains of the pathogen. Thus Xa27 specificity towards incompatible pathogens involves the differential expression of the R gene in the presence of the AvrXa27 effector.

Structured Information

Gene Name	AY986492
Description	2361 bp DNA linear PLN 24-JUN-2005.
Definition	Oryza sativa (indica cultivar-group) Xa27 (Xa27) gene, Xa27-IRBB27 allele, complete cds.
LocusTag	{{{tag}}}
Ensembl Transcript ID	{{{tid}}}
Ensembl Protein ID	{{{pid}}}
Length	2361 bp
Source	Oryza sativa Indica Group ORGANISM Oryza sativa Indica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 6
Location	Chromosome 6:1558..1899
Sequence Coding Region	1558..1899
Genome Context	<gbrowseImage1> name=NC_008395:1558..1899 source=RiceChromosome06 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008395:1558..1899 source=RiceChromosome06 preset=GeneLocation </gbrowseImage2>
Coding Sequence	{{{CDNA}}}
Protein Sequence	<aaseq>MADWAMHHYLLLANQQRHRALADVAVRRRQLLLDSGRVFMLLGA VILMHMLTTTGGGASSGCTRGAEPCVALLLWLLGAALAMLSLVAGRFPVLAAAIAEEL GDHLLGGLWSL</aaseq>
Gene Sequence	<dnaseqindica>1 ctgcagctga accaaacagt tttagctcca tcgaagaaag gagttatact gattggaatg 61 ctctcacagt aaaaaaaaca aggaagtaga gctggatttt agacagttct acaagaagtt 121 agaactctac caaaattgga attttggatg atggtctttt aaaaactcga ttgcaggaat 181 aaaattttac ggcttgaaac ttacaaaatg attagaaaag ataacatgcc tcagcgattt 241 gtaaaaaagt gaacaaataa aaatctacaa taccactaaa ctattgcttt attttgggga 301 cattgcttac cattgaaaaa acaactaacc gtaaatacga acacccatat caaatatact 361 atcactgata aaataatcaa ttgtaaattc aagcacacat attagtatag tactttaact 421 cgattggata gaagaaacct aactaattta agctatgcct cacaacaaaa aggtataaat 481 tttttaaggc ttcttttttt ttcttgcgtt tgctagttta tgcttttaag atgtttatac 541 cttttactcc cctcattcac tgtttaaata caatgggaat tagtgaaatc aatgagagtt 601 caaacttcga aacactgaat acatgttatt ttggattgaa atcaaatcga atcagtcaaa 661 ttcaaatagg aggaggaaca taggcattct tcctttcttc agcgggcacc attgaattca 721 gatactgctt cgcctagtct ctgtccaaga ctccacattt tctgatggtg atggggaact 781 ctgaaactat aggaggaaga ataaaatgaa gaatgcagaa atgaatagta atttgtgttt 841 tttaattctt cttcaattcc accttaggat ccaacttcag tccaaatcca aagtaatgca 901 actgccacta gatcaggcta gagcttcaaa ttcaactcca aaaacctccg taaagtggca 961 cacacagagg aaaaatcctg gattcgtcac tgcccatcaa catctgcttt cgcctcccaa 1021 ttcctgcttt ctgaaatctg ctttcgccga attcatgcct tcttgaatta tgctttctta 1081 gaccctcttt agatgggact aaaactttta ctctctatca catcggatgt ttggacacta 1141 attataaata ttaaacgtag actattaata aaacccatct ataatcttgt attaattcgc 1201 gagacgaatc tattgagcct aattaatcca tgattagcct atgtgatgct ataataaaca 1261 ttctctaatt ataaattaat tgggcttaaa aaatttgtct cgcgtattag ctttcattta 1321 tataattagt tttataaata gtctatattt aatactctaa attagtgtct aaatacaggg 1381 actaaagtta agtcactgga tccaaacacc acctaaggtt ttcttgtgta cttgtgaatt 1441 gtggttgact acgactacta gtgctataaa tagaagaaga gacccataga gagcatcaga 1501 gcaaagtact cctaaaagac agccacacac actgagacac ccaagaagct gcctccaatg 1561 gcggattggg cgatgcacca ctacctccta ctagccaacc agcaacgcca ccgagccctc 1621 gccgacgtcg ccgtccgccg ccgccagctg ctcctcgact ccggccgcgt cttcatgctc 1681 ctcggcgccg tcatcctcat gcacatgctc accactaccg gcggcggagc atcgtccggc 1741 tgcacccgcg gcgccgaacc ttgcgtcgcc ctcctcctgt ggctgctcgg cgcggcgctc 1801 gccatgctgt cgctcgtcgc cggccgattc cccgttctcg ctgccgccat tgctgaggag 1861 ctcggtgatc acctgcttgg tggtctctgg tctctctagt tctcctccgt gtccggtggt 1921 catcttcttc tccgtgcttt tgctctggag ttgagtacgg atctgtgtgt actgcattct 1981 tgcttaatta gtgccctaca cgttatgctt tcgaaacatc atcttttttc agtatagttc 2041 aataaatttc agctcaaatt tgtcctccaa gacgagttct ccatccaaac gaaacttatg 2101 gtgttccgtt gtttgggccg attttatatg ttggaaatgt acagacttca tagtactgtg 2161 tttctttttt ggaataagtt caccagaggt tccttaactt aacggcgata tttttttagg 2221 tcctttaacc acaaaaccag aaatgtgcac ccctaaactt tcacaatccg tgcacaagag 2281 gtcctatggc agtatacgtg ggtggtttcg ctgacgtgac atcctagtca gcaaaaataa 2341 ataaataagt aagtggggcc c</dnaseqindica>
External Link(s)	{{{Link}}}

Labs working on this gene

1. Temasek Life Sciences Laboratory, National University of Singapore, Singapore, 117604, Singapore

2. Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, Anhui, China

2. Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, Anhui, China

References

1. Yanchang Luo;Jatinder S. Sangha;Shouhai Wang;Zefu Li;Jianbo Yang;Zhongchao Yin

 Marker-assisted breeding of Xa4, Xa21 and Xa27 in the restorer lines of hybrid rice for broad-spectrum and enhanced disease resistance to bacterial blight
 Molecular Breeding, 2012, 30(4): 1601-1610

2. Keyu Gu;Bing Yang;Dongsheng Tian;Lifang Wu;Dongjiang Wang;Chellamma Sreekala;Fan Yang;Zhaoqing Chu;Guo-Liang Wang;Frank F. White;Zhongchao Yin

 R gene expression induced by a type-III effector triggers disease resistance in rice
 Nature, 2005, 435: 1122-1125

3. K.Gu;D.Tian;F.Yang;L.Wu;C.Sreekala;D.Wang;G.-L.Wang;Z.Yin

 High-resolution genetic mapping of Xa27(t), a new bacterial blight resistance gene in rice, Oryza sativa L.
 Theoretical and Applied Genetics, 2004, 108(5): 800-807