Os04g0301500

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Annotated Information

The RERJ1 cDNA is 1353 bp in length, with an open reading frame (ORF) encoding a putative polypeptide of 310 amino acid residues. The nucleotide sequence data for the RERJ1 cDNA reported here will appear in the GSDB, DDJB, EMBL, and NCBI nucleotide sequence databases under Accession No. AB040744. The putative amino acid sequence of RERJ1 contains a bHLH motif characteristic of a superfamily of transcriptional regulators The entire amino acid sequence of RERJ1 shares less than 20% identity with bHLH proteins functionally characterized so far.

Introduction

Jasmonic acid (JA) is a plant hormone that regulates various developmental processes in plants and also acts as a signal molecule in plant defense against herbivores and pathogens. Treatment of suspension-cultured rice cells with a chitin elicitor (N-acetylchitoheptaose) induces the production of phytoalexins. JA is a key signal transducer between the recognition of N-acetylchitoheptaose and the production of the phytoalexin momilactone A in rice cells. Screened for cDNAs for early JA-responsive genes using a cDNA library constructed from suspension-cultured rice cells treated with JA for 1/2 h. This resulted in the isolation of a cDNA for RERJ1, which is also induced by treatment with JA. It was suggested that RERJ1 encodes a novel transcriptional regulator with a basic helix–loop–helix (bHLH) motif. Basic HLH proteins, which have been identified in all eukaryotes examined to date, form either homo- or hetero-dimers. These proteins constitute a superfamily of transcription factors that has been extensively characterized in non-plant eukaryotes, in particular in mammalian systems, in which they have important roles in neurogenesis, myogenesis, cell proliferation and differentiation, cell lineage establishment, sex determination,and other essential processes .

Function

Please input function information here. RERJ1, a JA-inducible transcription factor in rice, is up-regulated by exposure to wounding and drought stress.RERJ1 has self-activating ability. It is a upregulated genes in plants under the drought stress and the mechanical damage.And after mechanical damage, its expression is affected by a JA-dependent manner. RERJ1 only induced in place where mechanical damaged, but the drought make it expressed throughout the leaf area. Therefore, RERJ1 as a transcriptional activator can regulate gene expression induced by stress, and this stress related with JA accumulation areas.RERJ1 plays a role as a transcriptional activator for regulating stress-inducible gene expression, with a strong correlation to JA accumulation in the stressed region. The plant bHLH proteins that have been characterized to date function in transcriptional regulation associated with several physiological phenomena, including anthocyanin pigmentation , DELILA in snapdragon. The biological functions of most plant bHLH proteins remain unclear.The molecular cloning and characterization of the JA-responsive rice gene RERJ1, which encodes a bHLH protein.

Expression

Please input expression information here. The expression of RERJ1 after wounding is regulated in a JA-dependent manner in rice. RERJ1 expression was induced only at the region of injury after wounding, whereas expression was induced in the entire leaf after drought. According to JA measurements of stressed leaves, high accumulation of endogenous JA was only detected around the wound site in a rice leaves, whereas the drought treatment led to uniform accumulation of JA in the entire leaf, suggesting that RERJ1 will be a useful marker gene for studies on localization of JA in rice. It was suggested that RERJ1 encodes a novel transcriptional regulator with a basic helix–loop–helix(bHLH) motif.

Evolution

Please input evolution information here. AN1 and JAF13 in petunia GMYC1 in gerbera anthocyanin pigmentation/trichome differentiation in Arabidopsis, phytochrome signaling(PIF3 and HFR1), globulin expression (PG1in Phaseolus vulgaris) , and symbiotic ammonium transport.

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Labs working on this gene

Please input related labs here.  Plant materials  Microscopic observation  Scanning electron microscopy observation  Positional cloning  Promoter activity analysis  Subcellular localization  Real-time quantitative PCR  mRNA in situ hybridization

References

Please input cited references here. 1.Koji Miyamoto;Takafumi Shimizu;Susumu Mochizuki;Yoko Nishizawa;Eiichi Minami;Hideaki Nojiri;Hisakazu Yamane;Kazunori Okada

 Stress-induced expression of the transcription factor RERJ1 is tightly regulated in response to jasmonic acid accumulation in rice
 Protoplasma, 2013, 250(1): 241-249

2.J.G. Turner, C. Ellis, A. Devoto, The jasmonate signal pathway,Plant Cell 14 (2002) S153–S164. 3.Kyoko Kiribuchia, Miho Sugimoria, Masayoshi Takedaa, Takashi Otani ,RERJ1, a jasmonic acid-responsive gene from rice, encodes a basic helix–loop–helix protein,Biochemical and Biophysical Research Communications 325 (2004) 857–863 4.M. Sugimori, K. Kiribuchi, C. Akimoto, T. Yamaguchi, E.Minami, N. Shibuya, H. Sobajima, E.-U. Cho, N. Kobashi, H.Nojiri, T. Omori, M. Nishiyama, H. Yamane, Cloning and characterization of cDNAs for the jasmonic acid-responsive genes RRJ1 and RRJ2 in suspension-cultured rice cells, Biosci. Biotechnol.Biochem. 66 (2002) 1140–1142. 5.C. Murre, P.S. McCaw, D. Baltimore, A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless,MyoD, and myc proteins, Cell 56 (1989) 777–783. 6.W.R. Atchley, W.M. Fitch, A natural classification of the basic helix–loop–helix class of transcription factors, Proc. Natl. Acad.Sci. USA 94 (1997) 5172–5176. 7.V. Ledent, O. Paquet, M. Vervoot, Phylogenetic analysis of the human basic helix–loop–helix proteins, Genome Biol. 3 (2002) 1–18.

Structured Information

Gene Name	Os04g0301500
Description	Basic helix-loop-helix dimerisation region bHLH domain containing protein
Version	NM_001058941.1 GI:115457611 GeneID:4335417
Length	2402 bp
Definition	Oryza sativa Japonica Group Os04g0301500, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 4
Location	Chromosome 4:13515243..13517644
Sequence Coding Region	13515513..13515611,13516595..13517065,13517150..13517429,13517497..13517579
Expression	GEO Profiles:Os04g0301500
Genome Context	<gbrowseImage1> name=NC_008397:13515243..13517644 source=RiceChromosome04 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008397:13515243..13517644 source=RiceChromosome04 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atggacgccgagatggccatgggcgagagcttcgcctactactgggagacgcagcgctaccttgagtccgaggagctcgacagcatgtacctgccgacgcaggatgactccaactacgagtcgagctcgccggacgggtcgcactcgtcgtcggcgccggcgccggcggccgtgggcggggacgcagccgccgcggtggcgggtagcggtggcgggatgacgacgatgatgatgggcggcggcggcggcggtggcgacgacgccggtggcgcgaacaagaacatcctcatggagcgcgaccgccgccgcaagctcaacgagaagctctacgcgctccgcagtgtcgtgcccaacatcaccaagatggacaaggcgtcgatcatcaaggacgcgatcgagtacatccagcgcctgcaggcggaggagcagcagatgctccgggaggtcgccgcgctcgagtccgccgccgcggcgagcgccgccccggccgcggcgaacccgttcgccggcctcggcgccgacgaggaacacgagtacggccaccaccacccctcgtcgtcgtcggagcggacgaagaaggtgaagcgggcgctgtccgtgtcgtccatcagcgacgccctgctcgccgcggcggcgccggcgccgccggtggagatccaggagctgcgcgtgtcggaggtgggcgacagggtgctggtggtgagcgtgacgtgcagcaagcgccgcgacgccatggcccgggtgtgccgcgccctcgaggagctccgcctccgcgtcatcaccgccaacatcacctccgtcgccggctgcctcatgcacaccctcttcgtcgaggtagatcacatggatagtgtccaaatgaagcagatggtggaggctgctctctcccagcttgtggcgaccggcagtccgttgagctccatgagctattag</cdnaseq>
Protein Sequence	<aaseq>MDAEMAMGESFAYYWETQRYLESEELDSMYLPTQDDSNYESSSP DGSHSSSAPAPAAVGGDAAAAVAGSGGGMTTMMMGGGGGGGDDAGGANKNILMERDRR RKLNEKLYALRSVVPNITKMDKASIIKDAIEYIQRLQAEEQQMLREVAALESAAAASA APAAANPFAGLGADEEHEYGHHHPSSSSERTKKVKRALSVSSISDALLAAAAPAPPVE IQELRVSEVGDRVLVVSVTCSKRRDAMARVCRALEELRLRVITANITSVAGCLMHTLF VEVDHMDSVQMKQMVEAALSQLVATGSPLSSMSY</aaseq>
Gene Sequence	<dnaseqindica>2034..2132#580..1050#216..495#66..148#gtcggagagagacacaagccaacctcgaactcgaagcagagcaaacgcgaagcagagcaagcgccatggacgccgagatggccatgggcgagagcttcgcctactactgggagacgcagcgctaccttgagtccgaggagctcgacaggtgcgcctccgcctcgccgccgccgccgatccgccattgttgttgctgacatggtgtttggttgcagcatgtacctgccgacgcaggatgactccaactacgagtcgagctcgccggacgggtcgcactcgtcgtcggcgccggcgccggcggccgtgggcggggacgcagccgccgcggtggcgggtagcggtggcgggatgacgacgatgatgatgggcggcggcggcggcggtggcgacgacgccggtggcgcgaacaagaacatcctcatggagcgcgaccgccgccgcaagctcaacgagaagctctacgcgctccgcagtgtcgtgcccaacatcaccaaggtaagggagagaggagtagaggtgttcgtccatggcggcgacggcgacattgctgactgactgctcgtcggtggttgggcgcagatggacaaggcgtcgatcatcaaggacgcgatcgagtacatccagcgcctgcaggcggaggagcagcagatgctccgggaggtcgccgcgctcgagtccgccgccgcggcgagcgccgccccggccgcggcgaacccgttcgccggcctcggcgccgacgaggaacacgagtacggccaccaccacccctcgtcgtcgtcggagcggacgaagaaggtgaagcgggcgctgtccgtgtcgtccatcagcgacgccctgctcgccgcggcggcgccggcgccgccggtggagatccaggagctgcgcgtgtcggaggtgggcgacagggtgctggtggtgagcgtgacgtgcagcaagcgccgcgacgccatggcccgggtgtgccgcgccctcgaggagctccgcctccgcgtcatcaccgccaacatcacctccgtcgccggctgcctcatgcacaccctcttcgtcgaggtataatacaatctccatctccattgctgacgtcacgcacgatcactgagctcctcttcctttccttaggtttgaccttccaagtttgactttgaccgttcacacgcagtactacattttacttccatttttaggtagaaaaataacgttgcgatcacacgctgctccctttgcggcgtcagtatgttaggccaatcaaatcctcaccaatcctaaagcaaccgtcccgaatctcaaagcatcagaaaatccccttctccccgctttacgaatccacctgattcttcttcttctgttcttcgattgcaaaaagcgattgaaaaccacatgggattaatcgggtgcgcacgagggagaaatcgaacaattcgatggatggatggatcgcgcacgtgcttcagataataattaatgaattaattttttgggggacaaacgggagcactacatctacatgggcgtcacatcacgtgcgtgttttggttgggggaagccatggaaaacgattggcacgtgttcgattgcacgggagatgatttttttcccctcgtttttttatttgttgtgacgttaaattccacacttttctttaagctgatttgattacagatgtgaagacaacagtttttgggggatgttctgtgttttgtcctcttctagaaattaggtgtttgatacacattattgaggccgagttagttttttttttttggggattgaggctgagttagtttgttgacttccacgtgcatgcacgtttcttgaactgttaaatttttaaaagaaattatacaaaaattgttttaaataaatcataacaatatattttttaagatttttaaaatatatttaattaatcatgtgttaatccattactctattttacgtgcggagggaaggggttcgcaacctcttcaaaagaacaaaaccttagcattgttaattttggggttttggctgaataaagttgtttcaaatttcaggtagatcacatggatagtgtccaaatgaagcagatggtggaggctgctctctcccagcttgtggcgaccggcagtccgttgagctccatgagctattagttatgagctggatagatatatggagtcatgcgttttggccacctactttacctagtctcattcgcaataaagcgcatttttatgtatgcttggtgatgttgttgttgtcatttttgcgacaccccactacgtgatggaggtggttgttttcttgcgaggcaacgatttagtggctggatgatagatttaagtattttggaaatcttgagtacgctgacttgtttatgtagctattaatattgatgaataataaggtggtccagcttaatg</dnaseqindica>
External Link(s)	NCBI Gene:Os04g0301500, RefSeq:Os04g0301500