Difference between revisions of "Os11g0117400"
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radiation. The transient expression analysis of the OsWRKY89–eGFP reporter in onion epidermal cells revealed that OsWR KY89 was targeted to nuclei. Transcriptional activity assays of OsWRKY89 and its mutants fused with a GAL4 DNA binding domain indicated that the 67 C-terminal amino acids were required for the transcrip tional activation and that the leucine zipper region at the N-term inus enhanced its transcriptional activity. Overexpression of OsWRKY89 led to growth retardation at the early stage and reduction of internode length. Scanning electron microscopy revealed an increase in wax deposition on leaf surfaces of the OsWRKY89 overexpression lines and a decrease in wax loading in the RNAi-me diated OsW RKY89 suppression lines. Moreover, extractable and cell-wall-bound phenolic compounds were decreased in the overexpressor lines, but its SA levels were increased. Lignin staining showed an increase in lignification inculms of the overexpressor lines. Intere stingly, overexpression of the OsWRKY89 gene enhanced resistance to the rice blast fungus and white-backed planthop per as well as tolerance to UV-B irradia-tion. These results suggest that OsW RKY89 plays an important role in response to biotic and abiotic stresses<ref name="ref1" />. | radiation. The transient expression analysis of the OsWRKY89–eGFP reporter in onion epidermal cells revealed that OsWR KY89 was targeted to nuclei. Transcriptional activity assays of OsWRKY89 and its mutants fused with a GAL4 DNA binding domain indicated that the 67 C-terminal amino acids were required for the transcrip tional activation and that the leucine zipper region at the N-term inus enhanced its transcriptional activity. Overexpression of OsWRKY89 led to growth retardation at the early stage and reduction of internode length. Scanning electron microscopy revealed an increase in wax deposition on leaf surfaces of the OsWRKY89 overexpression lines and a decrease in wax loading in the RNAi-me diated OsW RKY89 suppression lines. Moreover, extractable and cell-wall-bound phenolic compounds were decreased in the overexpressor lines, but its SA levels were increased. Lignin staining showed an increase in lignification inculms of the overexpressor lines. Intere stingly, overexpression of the OsWRKY89 gene enhanced resistance to the rice blast fungus and white-backed planthop per as well as tolerance to UV-B irradia-tion. These results suggest that OsW RKY89 plays an important role in response to biotic and abiotic stresses<ref name="ref1" />. | ||
| − | [[File:图片1.png]]Expression of | + | [[File:图片1.png]]Expression of OsWRKY89was induced by phytohormones and various stresses. (A)RNA gel-blot analysis ofOsWRKY8 9 expression in rice. Total RNA |
| − | OsWRKY89was induced by | ||
| − | phytohormones and various | ||
| − | stresses. (A)RNA gel-blot | ||
| − | analysis ofOsWRKY8 9 | ||
| − | expression in rice. Total RNA | ||
was isolated from leaves of rice | was isolated from leaves of rice | ||
seedlings subjected to different | seedlings subjected to different | ||
Revision as of 03:19, 30 May 2014
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Contents
Annotated Information
Function
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WRKY genes are a family of transcriptional regulatory factors that have specific functions in plants.Plant WRKY gene-encoded transcriptional regulators appear to be involved in various physiological programs, including disease-resistance,senescence,stress responses of biotic and abiotic, growth and development processes[1].
Expression
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Expression of OsW89p::GUS was observed in leaves, stems, and sheaths; pollens hadn't expression; mature seeds hadn't expression; the embryos of germinating seeds had expression; primary roots, adventitious roots and their lateral roots had expression but not in root tips of seedling stage; only lateral roots had expressin in roots of maturation phase[1].
Analyzing inducible expression pattern in T1 transgenic seedlings, the results showed: expression level of OsW89p::GUS can be increased by MeJA, IAA, ultraviolet, high temperature, low temperature and wounding treatment, inducible effects of MeJA and ultraviolet were distinct; and can be restrined by 2, 4-D; ABA and SA can't influence OsW89p::GUS expession; NaCl and PEG can restrain expression level of OsW89p::GUS in roots but increase expression level in leaves.GUS activity of OsW89p::GUS was higher than 35s::GUS in trangenic plants.In conclusion,we thought OsWRKY89 gene promoter was an inducible promoter with tissue specific expression characteristic[1].
RNA gel blot analysis indicated that OsWRK Y89 was strongly induced by treatments of methyl jasmonate and UV-B radiation. The transient expression analysis of the OsWRKY89–eGFP reporter in onion epidermal cells revealed that OsWR KY89 was targeted to nuclei. Transcriptional activity assays of OsWRKY89 and its mutants fused with a GAL4 DNA binding domain indicated that the 67 C-terminal amino acids were required for the transcrip tional activation and that the leucine zipper region at the N-term inus enhanced its transcriptional activity. Overexpression of OsWRKY89 led to growth retardation at the early stage and reduction of internode length. Scanning electron microscopy revealed an increase in wax deposition on leaf surfaces of the OsWRKY89 overexpression lines and a decrease in wax loading in the RNAi-me diated OsW RKY89 suppression lines. Moreover, extractable and cell-wall-bound phenolic compounds were decreased in the overexpressor lines, but its SA levels were increased. Lignin staining showed an increase in lignification inculms of the overexpressor lines. Intere stingly, overexpression of the OsWRKY89 gene enhanced resistance to the rice blast fungus and white-backed planthop per as well as tolerance to UV-B irradia-tion. These results suggest that OsW RKY89 plays an important role in response to biotic and abiotic stresses[2].
Expression of OsWRKY89was induced by phytohormones and various stresses. (A)RNA gel-blot analysis ofOsWRKY8 9 expression in rice. Total RNA
was isolated from leaves of rice
seedlings subjected to different
treatments at different time
points. Equal loading of 10lg
total RNA to each lane was
checked by ethidium bromide
staining.
32
P-labeled cDNAs of
OsWRKY89, OsBBPI, or PAL
fragments were used for
hybridizations. Rice seedlings
were treated with
phytohormones ABA (100lM)
and MeJA (100lM), or with
UV-B and mechanical
wounding. MES buffer, or 0.1%
methanol treatment was used as
the controls. (B–F)
Histochemical staining of the
transgenic rice seedlings
carrying theOsWRKY89
promoter-gusfusion construct.
The four-day-old rice seedling
(B) and its section (D), the four-day-old rice seedling treated
with UV-B for 15 min and
sampled 6 h after the treatment
(C) and its section (E) were
stained. (F)GUS staining of a
3-week-old leaf. (G)transverse
section of a root, showing
expression ofGUSin the
epidermal cells. Bars = 0.5 cm
in (B, C) and 0.1 cm in (D–G)
Evolution
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Knowledge Extension
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You can also add sub-section(s) at will.
WRKY genes encode transcription factors that are involved in the regulation of various biological processes. These zinc-finger proteins, especially those members mediating stress responses, are uniquely expanded in plants[3].
The WRKY gene family, important for plant development and responses to both biotic and abiotic stresses, has encoun-tered several duplication and deletion events in very recent evolutionary history in Oryza sativa. Completing the annotation of this family in rice will enable the determination of whether these changes are simply neutral occurrences in a heavily redundant family of transcription factors or whether they ac-tively contributed to the variation between modern rice sub-species and cultivars. Mounting evidence indicates that WRKY genes modulate the signaling networks for all hormones and regulate the biosynthesis of starch, sesquiterpene, and alka-loids in a variety of plants, including important crops. There is no doubt that studies of this family of transcription factors will not only further our understanding of the fundamental pro-cesses that are controlled by WRKY genes, but also help en-hance agricultural productivity[3].
Labs working on this gene
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Department of Plant Pathology, State Key Laboratory of Agrobiotechnology, China Agricultural University, Yuanmenyuan West Rd. 2, Beijing 100094, China
Biotechnology Institute, Zhejiang University, Hangzhou 310029, China
Department of Biological Sciences, Hunan University of Science and Technology, Xiangtan 411201, China
Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China
Bioinformatics Core, School of Life Sciences, University of Nevada, Las Vegas, Nevada 89154, USA
College of Agriculture and Biotechnology Zhejiang University, Hangzhou, P. R. China May. 2005
References
- ↑ 1.0 1.1 1.2 郝中娜 (2005). 水稻 WRKY19 和 WRKY89 基因启动子的分析, 浙江大学.
- ↑ Haihua Wang;Junjie Hao;Xujun Chen;Zhongna Hao;Xia Wang;Yonggen Lou;Youliang Peng;Zejian Guo Overexpression of rice WRKY89 enhances ultraviolet B tolerance and disease resistance in rice plants Plant Molecular Biology, 2007, 65(6): 799-815.
- ↑ 3.0 3.1 Christian A. Ross;Yue Liu;Qingxi J. Shen The WRKY Gene Family in Rice (Oryza sativa) Journal of Integrative Plant Biology, 2007, 49(6): 827-842.
Cite error: <ref> tag with name "ref3" defined in <references> is not used in prior text.
Structured Information
| Gene Name |
Os11g0117400 |
|---|---|
| Description |
DNA-binding WRKY domain containing protein |
| Version |
NM_001072123.2 GI:297611109 GeneID:4349611 |
| Length |
1015 bp |
| Definition |
Oryza sativa Japonica Group Os11g0117400, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 11:781474..782488 |
| Sequence Coding Region |
781474..781891,781997..782104,782198..782463 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008404:781474..782488 source=RiceChromosome11 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008404:781474..782488 source=RiceChromosome11 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgatagagcaccaaaaggctctcatggtggagctgcgtggcatggtcatgccattacttccgagcgacaatgaacaagccaagcttgctcttcaactcttgggagatatattgagttgctcagataaggctatctccatgctagaacttggtggagacactaaaaagctgaccaatcttgttggaggtaagagaaaaggtgacaagcatagcatggacaaccacaacttggaggaggaagccaaagaaagtgttagcaagagaagaaagaatgcagaacacacaggttcaactgtggctcaagcacctcacaatgatggacatcaatggaggaagtatggtcagaaatggatctctagagcaaaacattccaggagctactataggtgtgccaatagtaaagtgcaaggctgtcctgctacaaagacagtgcaacaaatggattccagtggaaatggaacatcaaagttgttcaacgttgactactatggccaacacacatgcaggggagatggcatagccgatccatatgttgtcgacacagcccatcacagtatggaacctatcaatcaaaacgaatgcaatagccctacacttgaacacgaagcccatgaagttcaagatgaaagatttgaaaacttgtgtatggtacaaaatatgccagagtatttgatagattttgaattggagagagccttcgagtttattgtgaactcaccattgggttctgagcattggacgttcgatgattcaataagatgtgagcacagtccaatatgcatatggggatga</cdnaseq> |
| Protein Sequence |
<aaseq>MIEHQKALMVELRGMVMPLLPSDNEQAKLALQLLGDILSCSDKA ISMLELGGDTKKLTNLVGGKRKGDKHSMDNHNLEEEAKESVSKRRKNAEHTGSTVAQA PHNDGHQWRKYGQKWISRAKHSRSYYRCANSKVQGCPATKTVQQMDSSGNGTSKLFNV DYYGQHTCRGDGIADPYVVDTAHHSMEPINQNECNSPTLEHEAHEVQDERFENLCMVQ NMPEYLIDFELERAFEFIVNSPLGSEHWTFDDSIRCEHSPICIWG</aaseq> |
| Gene Sequence |
<dnaseqindica>598..1015#385..492#26..291#tagtgactgtcaagtagtgatcaacatgatagagcaccaaaaggctctcatggtggagctgcgtggcatggtcatgccattacttccgagcgacaatgaacaagccaagcttgctcttcaactcttgggagatatattgagttgctcagataaggctatctccatgctagaacttggtggagacactaaaaagctgaccaatcttgttggaggtaagagaaaaggtgacaagcatagcatggacaaccacaacttggaggaggaagccaaagaaagtgttagcaagagaaggtatatgtatataaatacatgccactagaggaatgtccactagcttatcattcattgctaacgaaatttgattgatcctcttgattatgatagaaagaatgcagaacacacaggttcaactgtggctcaagcacctcacaatgatggacatcaatggaggaagtatggtcagaaatggatctctagagcaaaacattccaggtataaatgagcatatgaactcctgtttatttccattcctcgaagcatattggttttatctaacagcaaaatatatacatctaatgagctcatttgcttaagcaggagctactataggtgtgccaatagtaaagtgcaaggctgtcctgctacaaagacagtgcaacaaatggattccagtggaaatggaacatcaaagttgttcaacgttgactactatggccaacacacatgcaggggagatggcatagccgatccatatgttgtcgacacagcccatcacagtatggaacctatcaatcaaaacgaatgcaatagccctacacttgaacacgaagcccatgaagttcaagatgaaagatttgaaaacttgtgtatggtacaaaatatgccagagtatttgatagattttgaattggagagagccttcgagtttattgtgaactcaccattgggttctgagcattggacgttcgatgattcaataagatgtgagcacagtccaatatgcatatggggatga</dnaseqindica> |
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