Difference between revisions of "Os09g0395300"
Chenyaping (talk | contribs) |
Chenyaping (talk | contribs) |
||
| Line 8: | Line 8: | ||
===Expression=== | ===Expression=== | ||
| − | + | [[File:ZhangF5.jpg|right|thumb|200px|Fig 5. ''SLL1'' Expression Pattern Analysis <ref name=''ref1'' />.]] | |
| − | + | Quantitative real-time RT-PCR (qRT-PCR) analysis revealed the expression of ''SLL1'' in various tissues, including roots, stems, leaves, flowers, and seedlings, with relatively high expression in leaf and seedlings (Figure 5A , top panel). With an emphasis on the leaf, analysis by qRT-PCR showed that ''SLL1'' is ubiquitously expressed in shoot apical meristem (SAM), leaf blade, or leaf sheath at different developmental stages (Figure 5A, bottom panel). | |
| − | The spatial and temporal localization of SLL1 during leaf development was further examined by in situ hybridization analysis. The mRNA expression of SLL1 was detected throughout the young leaf primordium (plastochrons 1 to 3) and was more intense in abaxial cell layer through leaf development (plastochrons 4 and 5) (Figure 5D, panel 2). However, the SLL1 transcript did not demonstrate apical/basal polarity and did not accumulate at the apex of the meristem (Figure 5D, panel 3). Cross-section analysis of the shoot apex showed that SLL1 was more highly expressed in the abaxial cell layer, including the epidermis and vasculature of the early leaf blade (Figure 5D, panels 5 and 6). In the mature leaf, SLL1 mainly accumulated at the abaxial epidermis, abaxial mesophyll cells, and vasculature (Figure 5D, panels 8 and 9). In contrast with the sense probe (Figure 5D, panels 4 and 7), SLL1 was transcribed at a relatively low level throughout other leaf positions including the adaxial epidermis (Figure 5D, panels 5 and 6). | + | To assess the expression pattern comprehensively, β-glucuronidase (GUS) activity was examined histochemically in transgenic plants carrying an ''SLL1'' promoter-GUS reporter gene. Results showed that ''SLL1'' was transcribed in stem (Figure 5B, panels 1 and 2), anthers of young or mature flowers (Figure 5B, panels 3 to 5), pistil tip (Figure 5B, panel 6), glume (Figure 5B, panel 7), vascular tissues of mature seeds (Figure 5B, panels 8 and 9), coleoptile and embryonic root of germinating seedlings (Figure 5B, panel 10), and root vascular tissues (Figure 5B, panel 11). In addition, ''SLL1'' is highly transcribed in leaf veins and leaf sheath (Figure 5C, panels 1 to 3), guard cells, and tracheal elements (Figure 5C, panels 4 and 5). |
| + | |||
| + | The spatial and temporal localization of ''SLL1'' during leaf development was further examined by in situ hybridization analysis. The mRNA expression of ''SLL1'' was detected throughout the young leaf primordium (plastochrons 1 to 3) and was more intense in abaxial cell layer through leaf development (plastochrons 4 and 5) (Figure 5D, panel 2). However, the ''SLL1'' transcript did not demonstrate apical/basal polarity and did not accumulate at the apex of the meristem (Figure 5D, panel 3). Cross-section analysis of the shoot apex showed that ''SLL1'' was more highly expressed in the abaxial cell layer, including the epidermis and vasculature of the early leaf blade (Figure 5D, panels 5 and 6). In the mature leaf, ''SLL1'' mainly accumulated at the abaxial epidermis, abaxial mesophyll cells, and vasculature (Figure 5D, panels 8 and 9). In contrast with the sense probe (Figure 5D, panels 4 and 7), ''SLL1'' was transcribed at a relatively low level throughout other leaf positions including the adaxial epidermis (Figure 5D, panels 5 and 6). | ||
===Evolution=== | ===Evolution=== | ||
Revision as of 11:02, 30 May 2014
The rice SHALLOT-LIKE1 ( SLL1) gene, a member of KANADI family, is a key gene controlling rice leaf rolling.
Contents
Annotated Information
Function
sll1, a rice mutant with shallot-like Leaves, displays abnormal sclerenchymatous cell development in the abaxial cell layers, altered mesophyll cell distribution, increased amounts of chlorophyll, and enhanced photosynthesis (Fig. 1&2).
Expression
Quantitative real-time RT-PCR (qRT-PCR) analysis revealed the expression of SLL1 in various tissues, including roots, stems, leaves, flowers, and seedlings, with relatively high expression in leaf and seedlings (Figure 5A , top panel). With an emphasis on the leaf, analysis by qRT-PCR showed that SLL1 is ubiquitously expressed in shoot apical meristem (SAM), leaf blade, or leaf sheath at different developmental stages (Figure 5A, bottom panel).
To assess the expression pattern comprehensively, β-glucuronidase (GUS) activity was examined histochemically in transgenic plants carrying an SLL1 promoter-GUS reporter gene. Results showed that SLL1 was transcribed in stem (Figure 5B, panels 1 and 2), anthers of young or mature flowers (Figure 5B, panels 3 to 5), pistil tip (Figure 5B, panel 6), glume (Figure 5B, panel 7), vascular tissues of mature seeds (Figure 5B, panels 8 and 9), coleoptile and embryonic root of germinating seedlings (Figure 5B, panel 10), and root vascular tissues (Figure 5B, panel 11). In addition, SLL1 is highly transcribed in leaf veins and leaf sheath (Figure 5C, panels 1 to 3), guard cells, and tracheal elements (Figure 5C, panels 4 and 5).
The spatial and temporal localization of SLL1 during leaf development was further examined by in situ hybridization analysis. The mRNA expression of SLL1 was detected throughout the young leaf primordium (plastochrons 1 to 3) and was more intense in abaxial cell layer through leaf development (plastochrons 4 and 5) (Figure 5D, panel 2). However, the SLL1 transcript did not demonstrate apical/basal polarity and did not accumulate at the apex of the meristem (Figure 5D, panel 3). Cross-section analysis of the shoot apex showed that SLL1 was more highly expressed in the abaxial cell layer, including the epidermis and vasculature of the early leaf blade (Figure 5D, panels 5 and 6). In the mature leaf, SLL1 mainly accumulated at the abaxial epidermis, abaxial mesophyll cells, and vasculature (Figure 5D, panels 8 and 9). In contrast with the sense probe (Figure 5D, panels 4 and 7), SLL1 was transcribed at a relatively low level throughout other leaf positions including the adaxial epidermis (Figure 5D, panels 5 and 6).
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this gene
- State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China
- National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
References
<references> [2]
Structured Information
| Gene Name |
Os09g0395300 |
|---|---|
| Description |
Similar to CDPK substrate protein 1 |
| Version |
NM_001069632.1 GI:115479006 GeneID:4346973 |
| Length |
7672 bp |
| Definition |
Oryza sativa Japonica Group Os09g0395300, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 9:14411926..14419597 |
| Sequence Coding Region |
14411926..14412128,14413047..14413888,14413990..14414066,14414656..14414701,14414841..14415084 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008402:14411926..14419597 source=RiceChromosome09 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008402:14411926..14419597 source=RiceChromosome09 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggcaatggtgcgggagctcgagttgatgacgagctggagcaacagcatggggcgacaccgctacccaacgcgcatcctcgtcgattccttcgggcataagtgcagtgcctctgacaagggtgtgtggacctcctgcagcattcgcgcacccttgcaaggacgcggctccttccgacgtggcgcgaatattcgctttgggagcttgccgagcagcgcggcggtggcgacgtcggggggagggcgtggcggcggcggggtggtggtgggaggaggaggaggggacccgtggcggaggctggatgggtccacggcgtcgacggagttgtcgctgtcgccgccgccggcgcaggcggcgggtgggggaggtggtggtggtggagcggacgcgctgccgtggcggcaccgaccttcgccgccgtcgtcggcggtggccaccacgtctgccgccgccgcggcggcgctgatggcgccgatgatgctgcagccgctcgacgccggcggcggcgcgtcggcgccgccgccgccgatccgcgggatacctatctacaacggccccggcgggttcccgttcctgcagccgtcgcccaccgccggcgacgtcggccaccaccaccaccaccaccccaagatgggattctacagctcgtaccaccacccatccacgtggccctccacgtcgccgtccccgctcgcggcgccgccgggcgccgcgtcgtcgccgctcgaccccacggcggcgttcctctcctccccccaccaccggatgctgtccgccgcctcggggaggctcaacggcatgctctccgtctccgacaccctccgcagctacggcgtccccggcgccgccgcccccggcgtcatcggcggcgcgcaccaccaccaccaccacctccacggcggccagccgttcgtcggcgccctcgcgtcccgcttcatgcccaagctccccgccaagcgcagcatgcgcgcgccgcgcatgcgctggacgagcaccctccacgcccgcttcgtccacgccgtcgagctcctcggcggccacgagagggcgacgcccaagtcggtgctggagctcatggacgtcaaggatctgacgctagcgcatgtcaagagccacctccagatgtatcgcaccgtgaagagcactgacaagcctgcagcctcttcagggccggcggacggcggctccggcgacgaggagttcgccggcggcgggcaggcggcgtcgggcggcggcgacagcatgtgcctgaggggtggcggcggcggcggggtggccgcggcggcgttcgcggagcacggccggtcggcgtcggagggcgccgccagctcggtcggcggcggcggcggcggcgacatggaccagtcgtcggccggcaacaccagcaccaccaggtggagcaactcctcaagggacccatggctgtcgtccaattcttgcaacatggacgcccatcgctccgtaggattgtcttctcctattgaggttcttgcacttcctttagcctcattgatcatgtcagatactgcctacctgttcttttccagcaaccatggccggtgtgaaccggcctctcttgaccagaaactagcagatatctga</cdnaseq> |
| Protein Sequence |
<aaseq>MAMVRELELMTSWSNSMGRHRYPTRILVDSFGHKCSASDKGVWT SCSIRAPLQGRGSFRRGANIRFGSLPSSAAVATSGGGRGGGGVVVGGGGGDPWRRLDG STASTELSLSPPPAQAAGGGGGGGGADALPWRHRPSPPSSAVATTSAAAAAALMAPMM LQPLDAGGGASAPPPPIRGIPIYNGPGGFPFLQPSPTAGDVGHHHHHHPKMGFYSSYH HPSTWPSTSPSPLAAPPGAASSPLDPTAAFLSSPHHRMLSAASGRLNGMLSVSDTLRS YGVPGAAAPGVIGGAHHHHHHLHGGQPFVGALASRFMPKLPAKRSMRAPRMRWTSTLH ARFVHAVELLGGHERATPKSVLELMDVKDLTLAHVKSHLQMYRTVKSTDKPAASSGPA DGGSGDEEFAGGGQAASGGGDSMCLRGGGGGGVAAAAFAEHGRSASEGAASSVGGGGG GDMDQSSAGNTSTTRWSNSSRDPWLSSNSCNMDAHRSVGLSSPIEVLALPLASLIMSD TAYLFFSSNHGRCEPASLDQKLADI</aaseq> |
| Gene Sequence |
<dnaseqindica>1..203#1122..1963#2065..2141#2731..2776#2916..3159#6515..6587#7556..7672#atggcaatggtgcgggagctcgagttgatgacgagctggagcaacagcatggggcgacaccgctacccaacgcgcatcctcgtcgattccttcgggcataagtgcagtgcctctgacaagggtgtgtggacctcctgcagcattcgcgcacccttgcaaggacgcggctccttccgacgtggcgcgaatattcgctttgggaggtatgagactacgctccatggtggagcgttgggcgtgaccttagtgtgcgcatatgtaagctgtatgcgtacgtataaatgttgagcttgcagtacatgtatacttgtttttttcttcttttttatgaggtcctttaattccattgtctgtattatttatgctagtatggaggatgatggtggcagtgacataaagtatacaaaaagcatagtatagtatacgtatggaaacaaaagcatgctacctttgactccacttgcattgcctgctaactgccattatttaaggaatacatggttgaatgcctgcctaaacatcaagataacctcacattctcctctctctctctcctaccttgcaattaagggtagcagcagtactctcctcactgctggctgactgctcccattttctctctctactctctctctctctctctcctctattccttttatacttgttgttggtagctaggagtatagcaggacagcacaaggaaccaattcatgcatatatccctggtgtctctcatgcatgttagtgtgaagaaagaagaagcttgagagagaagagagagttagagagagaaagtagagtgtttgtagagagagaaagtggtgtgtgtactagtagtaactccaagccagtgctagagacagagatacaaaaaggagacatcatccttctcttggccaaagcaagcaaccaagcaaagcttcctcttcttctccaagaactccaagaaccttctccttcatcttctccaccatgattccttccatcgcatgagctccatctccggcgtgtcgatcgagatcaccgttgctgctgccgaggtggagaaggaggtgtttgatcgatcggtcgatggaggcgagctcggcggcgacgccaccggacctgtcgctgcacatcagcttgccgagcagcgcggcggtggcgacgtcggggggagggcgtggcggcggcggggtggtggtgggaggaggaggaggggacccgtggcggaggctggatgggtccacggcgtcgacggagttgtcgctgtcgccgccgccggcgcaggcggcgggtgggggaggtggtggtggtggagcggacgcgctgccgtggcggcaccgaccttcgccgccgtcgtcggcggtggccaccacgtctgccgccgccgcggcggcgctgatggcgccgatgatgctgcagccgctcgacgccggcggcggcgcgtcggcgccgccgccgccgatccgcgggatacctatctacaacggccccggcgggttcccgttcctgcagccgtcgcccaccgccggcgacgtcggccaccaccaccaccaccaccccaagatgggattctacagctcgtaccaccacccatccacgtggccctccacgtcgccgtccccgctcgcggcgccgccgggcgccgcgtcgtcgccgctcgaccccacggcggcgttcctctcctccccccaccaccggatgctgtccgccgcctcggggaggctcaacggcatgctctccgtctccgacaccctccgcagctacggcgtccccggcgccgccgcccccggcgtcatcggcggcgcgcaccaccaccaccaccacctccacggcggccagccgttcgtcggcgccctcgcgtcccgcttcatgcccaagctccccgccaagcgcagcatgcgcgcgccgcgcatgcgctggacgagcaccctccacgcccgcttcgtccacgccgtcgagctcctcggcggccacgagagtacgcccccgccatcatcgtcgtcgtcctcctcctccttctcaccaccatcatcaacgagctcgatcaatctgatgagaatccaatcttgttcttggcaggggcgacgcccaagtcggtgctggagctcatggacgtcaaggatctgacgctagcgcatgtcaagagccacctccaggtatcccgccattgccgaccaattctcacagctcgatcgatcgatcaaacaccactcaccactgcaccatctctctctttctctctctcgatttcgattccattcagcttctgttcttgatcatcttgtttgggtgagacaaagatgccaatgcagtaactcctgagttagtgaagaaactgccattcttgggagcagagagagagagtgtgtgtgtgacatgtttggggatgtgtgtgcaagagagaagagagagagagaggggaaaaaagttgccatcattacacaataatgcattgcatctgcatctgtgctactagctccttccttagctctagtcatactagcgtatatgtgcacggcccaaagcactctcacacacaaacacaagagagagagagagagagagaagaagaagatgagagatgagagagagtaggacatattttgtgtgtgtttctttgctgcttgtcttttgcaatggcttcaacctcctggtttcattttgatctcacaaaaaatgtgtgtgtgtgtgtaaatatatatattgatcttctttttgttggacttttgtgttttattactgcagatgtatcgcaccgtgaagagcactgacaagcctgcagcctcttcaggtgattttttttaccatggcagctactagtagtagtagtagtagctaatttagctaagctccattttgcttttctttttttttggcgttgtgcattgcgttttacattacattgttgctatggtttttttgacgtgtagggccggcggacggcggctccggcgacgaggagttcgccggcggcgggcaggcggcgtcgggcggcggcgacagcatgtgcctgaggggtggcggcggcggcggggtggccgcggcggcgttcgcggagcacggccggtcggcgtcggagggcgccgccagctcggtcggcggcggcggcggcggcgacatggaccagtcgtcggccggcaacaccagcaccaccaggtggagcaactcctcaaggtacacacatggcagagttgcatttcaagtctttcagattaattatatatctacattgttatgccatctttaattaattaaaaatgaaattaatcaatcagaatgtcatgagatgatgatgatgatgataataatactaggataggaagaggatgatatgatggatgggtgtgctatgtgtgtttttaagtgtgttttagcttagaaccaaaggcaacaccaacaaactttggcatgcattgatgatgtatctaaatatgagttagtgacatgcaaaattaacctgccaagtacaacctctatttattaattcccaaaaaaatctcatggaggagaagggagagagagagatagtagtaatgtgtggtgatgcatggctaaaaaagcttagcaaaagaatgttttgggggcacacacacacacacctggagagggagttgcctatgtttaggtggccaatgttgttatgtcacacaaaagaccaaaaagtcacacaccccatggcttggcctcctcctccccccttgacctgtgcagttagctaggcttcttctcttctctccctctgtgtttggtttttgtggagctaaaaacattttaaaaatcttgcaaacattgtgtgtaaaaataggttcttgggctctgcccattgtcctctagcttctctcaaatctctctctctctctctctctctctctctctctttctcaaacctagggaggggagggttactcaggcagggctccattatcatttcttatcaatgcatggctctgattccagggggatggggctttgacctgacatgccctattgcctaggaatatgtaaccaaggacttgctgcattcttggttctttctccctctctcaccagtctcttcaatgcccttttcttccacctcttgggcaattattagcatggatggagctcaccttttcactctaccctctagctgtgattaaatcattgactaatcctaattcataaatataagggattcaactatgtaactagatgtactagatcctagtattactagtgtgttttgattctgttaggaggagtgattagcgtttgtcgtaagtactagtattaaactataggggattagtgtagatcatttttttaaaaattagtgagtgtgcttgggcagattttactccagccattggcgtgaaaactttgatctttgggcagcttggaagcaagtacagttcaacctgtccttggctgcattcgctgctttttctttctcaggactcactgcaactttcttgccatccccaacacctccctttcggtcgtgtttgatcagttgcattgctggtttcttcgccgctttaatttctccatctttcttctgttccttattggctaagtaaaaaatcattctcagcaatcaagttttgaaattcaaattagcttcaggttttatctatctaataattctctgactagttctctttctttcaagacctgttcttgttcagaactcttatgcagttgagagttcagttgttcatggaagtaatggcaatgcctggtctagtctttgagcagtatcctccttatgctcttgattttccttcactttagccgtttcagtcaaactttggggacaaattaaagtcagctggtcaagtgcatacataccacgtcatattttttagatgaatccattgtagtattaaatactagtgacaaaaaaatataactaaatggcattaaattgcaaccttcttccccaaaataattgggcacaactaccttgcttgcatatgctttatagtactcctacttattacttccttgaatccatgaggattccaggaatttggtcaggtcaaatgtccaggaggaattaggctatctttttttatcttcttctttttcttctaggtagtaggatcgttgtgcacatataaacaaaacaaaatagaaatttaagaggaaaccctcccaaaaaaaatacgactatcaattatgagtgattttacagttcttgaggaggtacaatgatacattttctattttaaaattcagtaactttgatactatgaggtacagaaatttgcacaaaaaaaatttggtacctctcaagtacttaaaatatcttatcaattatatttcagcaatgttgtcgttgctcgagttgtgcaaaaacatttctgatgaacaagtggagatgtatgcatatttagagagggaaacacatgcattttttaatatacattctcatgacaaattttggtgttctcgtctcattttagtctagcttagattaatggcatccatatattggtgaactgaaacagattgtatatgtccagtcttgaacaaacttcattatacaatgcataatcagaaaagatacagacatatattggcagatcacataaagtggtgtatctatgattgggtggtattttggacatacaaagtctactggtccctcatttgttaacagcatacatttgttcctatcaagacaaaagcactttacaacagtttggtagtaagttggtgttatgtgcgtgtctatttagatagagtggtagtaaaggaaggacactttttcttgatataccaaaatcacacataaaaaagaagtgtcattttcatgatcaacatgtggggtatgaacaaaaatgttctcccccctccaaccaacattgaagtcatcacacataaaaagcaaaaacatttaacaataatacatgcagacacatgtgcatgcatgtgtggatcacgcaaggacatactaattcaattaggtattcacaattaattagtgcaggaaatgacatgtaattacaattatatatgcacaacgatccttgtgtatttgtgtgacccattgtagtagcatttgattcctttcttgtgcctttctctacattgaattcgatctttgccaacagcggcttgctgatgagctcctgtgccattgcactgctgagctgcaccaaacagggacatcttttaagagcttgttgcctggaaatgatccatgtaaccaactgttcaggaagagcacactgtttgctgctcactttagctaaagcaaaactgtgtgcattatgcaatgcatatgcatccttccatctacccctgtgtcctttgccccccaaactgtgtaactacagtttaccatgcacatacatacaatgcacatctacccaattgtagcattgttgcaaggcctacttcagaattcagttagtccccaattgatccaactgcacacatatacatttacatatacatatacacaaacataccagcttggagaaaaggttgtaacaactttgctaaatgtgagatgtgtaatgtgtgttcttcctcttttcagggacccatggctgtcgtccaattcttgcaacatggacgcccatcgctccgtaggattgtcttctcctattgaggtatatatccttacattgttcaaagcttctttcgattcctagctaactgcatatccatgcagaaattcattagctcgcattcattctgaacatgatgtctgaaactctgaattttttttcgtttgtgcgtgtttcagaacttggaaccgtgcagatcgagcagctcgcaggtgtccaaccatgagctgagtagccctagtctcgagttcactctagggaggcctgactggcacggtgcagatcatgattagatggcaatcacagaggctaatgatcaaaaaaaaatttctcctgagatgtgaaatatatatgtgatatacaccaacctggtgctgatcatctttcgccaaccccagcttagccagagccagttcgtctgaagcttgtcatggcgaagaatgatcagcagcaatggcttgaaacgcacatcatatccactgcgtcgtcgtcatcgtcgtcgcaatcgatcggagaagagatatatatttcgcgtctcgatctcatcatcacaatttgggttttcttgattccttcttcttcttctttttgcaattaatatcatggatgcagctagagtttccttgtctgatctccatatttatttagttttgttcgccgtgccgggatcgatttacagtgtagttcagtggtgagctagctagctagagagtgagtgagtgagtgtcagctaggcagctggagatcgagagtgtgagtttggtgatgagttggttgtgtgtttgtcagtgtgtgtgaaagtgtgtgtgccattagcacatggaaagttggaaaggagagaaaggaagaaagaaaagggaattgagttcaggtgaagcaatctgccaatctatctagctacctatctatccatctacttagctcagttgctcagatgatgatctcagatttccatggcccttttttgtgtgcatgcatgccgcatatgcttctttctttaatccctgcaggttcttgcacttcctttagcctcattgatcatgtcagatactgcctacctgttcttttccagcaaccatggccggtgtgaaccggcctctcttgaccagaaactagcagatatctga</dnaseqindica> |
| External Link(s) |