Os02g0799100

The Ossgo1gene encodes the protein OsSGO1 which maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis.

Annotated Information

Function

Os02g0799100 is the rice(Oryza sativa) homologue of the maize Sgo1 gene. It encodes an homolog of shugoshin protein ZmSGO1 in maize (Zea mays), named OsSGO1. The gene OsSGO1 is essential for rice meiosis and plays an important role in protecting centromeric cohesion during meiosis. The knockdown of OsSGO1 may cause precocious disassociation and random segregation of sister chromatids at telophaseⅠand anaphase II, respectively, which finally leads to sterile pollen formation.And the OsSGO1 localizes to centromere from the result of immunostaining experiments . In addition to the meiosisspecific maintenance of centromeric cohesion, OsSGO1 is required for the timely assembly and maintenance of SCs during early prophase I. Furthermore, the centromeric localization of OsSGO1 depends on OsAM1 . OsAM1 is the homolog of Arabidopsis SWI1 and maize AM1 in rice and is required for the leptotene–zygotene transition . OsSGO1 is specifically required to protect centromeric cohesion during meiosis.OsSGO1 transfers from nucleoli onto centromeres at the onset of prophase in both meiosis and mitosis.The relocalization of OsSGO1 onto centromeres is OsAM1-dependent.The maintenance of SCs in prophase I is affected in Ossgo1 .

Mutation

Schematic representation of OsSGO1 gene (from reference ).

An OsSGO1-RNAi vector is transformed into rice calli by Agrobacterium-mediated DNA transfer. OsSGO1-RNAi lines isobtained by screening the plants regenerated from the transformed calli by PCR. One single Tos17-insertion mutant line of the OsSGO1 gene, Ossgo1-1, is identified by screening the public insertion line collections. Sequence analysis of its PCR products confirmed that Tos17 is inserted into exon 15, only 5 bp upstream from the stop codon(Figure S1). .

Expression（Mutant VS Wild type）

Expression analysis of OsSGO1 (from reference ).

Characterization of the phenotype of Ossgo1 mutants (from reference ).

The defective cDNA sequence from Ossgo1-1 mutant (from reference ).

OsSGO1 is expressed most highly in the roots, secondly in the panicles, and at a relatively low level in leaves(Figure S3). The knockdown of OsSGO1 may cause precocious disassociation and random segregation of sister chromatids at telophase Ⅰand anaphase II, respectively, which finally leads to sterile pollen formation. The homozygous Ossgo1-1 mutant grows normally in the vegetative stage but is sterile during the flowering phase, and its pollen is completely non-viable when evaluated by 1% I2-KI solution staining (Figure S4). The transcription level of OsSGO1 is slightly decreased in the Ossgo1-1 mutant (Figure S3). Additionally, by performing RT-PCR, it is found that the Ossgo1-1 cDNA sequence is altered downstream of the Tos17-insertion position by the addition of 71 nucleotides, and the whole cDNA lacks a stop codon (Figure S5). Additionally, an allelic mutant of Ossgo1-1 with a deletion of 15 bp (nucleotides 1773–1787 in the gene) from the mutants induced by 60 Co~γ-ray radiation is identified and is named as Ossgo1-2 (Figure S1), which results in five amino acids missing in OsSGO1. The homozygous Ossgo1-2 mutant is also normal in the vegetative stage but completely sterile (Figure S4). Neither of the mutants set seeds when pollinated with pollen from the wild-type plants .

Primer	Forward primer	Reverse primer
Gene amplication	5'-CGAAACCTCATCGGATTCCT-3'	5'-GCCAATGGTGTTTGTGCTCT-3' (used to amplify the predicted coding regions of OsSGO1 )
	5'-ATTGTTAGGTTGCAAGTTAGTTAAGA'	5'-GCCTCGAACAAAGAGGACTG-3' (used to amplify the Tos17 inserted regions of ossgo1 )
	5'-GTGAATTCCCATTGAGGATCCAGAGCCACCA-3'	5'-AGTCTCGAGTACCTATCACCTGCTCGTCAGA-3' (used to amplify the fragment of OsSGO1 cDNA )
RT-PCR	5'-TCAATCAGCTGTGCCATCTT-3'	5'-CATCTTGCCACCACA AATCA-3'

Evolution

SGO1 gene is the first meiosis specificity expressed gene in yeast cDNA library screening conducted in Watanabe Lab, as well as to the lack of corresponding mutant phenotype analysis, identified as the homologous gene of Drosophila melanogaster Mei - S332 . Many researches of SGO function have been investigated in animals and yeast , while relatively few has been reported in plants. In plants, SGO1 gene is first found in maize, and the mutant of premature-dissociation centromeric cohesion which locates in the centromere at the stage of anaphase Ⅰ. And the phenotype is due to the deletion of ZmSGO1 protein. ZmSGO1 is found to be located in the centromere from the eptotene stage Previous studies indicates that ZmSGO1 plays an important role in protecting of centromeric cohesion during meiosis I .

Knowledge Extension

You can also add sub-section(s) at will.

Labs working on this gene

Please input related labs here.

Structured Information

Gene Name	Os02g0799100
Description	Hypothetical protein
Version	NM_001186253.1 GI:297721638 GeneID:9266998
Length	3610 bp
Definition	Oryza sativa Japonica Group Os02g0799100, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 2
Location	Chromosome 2:34917690..34921299
Sequence Coding Region	34920472..34920601,34920706..34920743,34920892..34921040,34921133..34921148
Expression	GEO Profiles:Os02g0799100
Genome Context	<gbrowseImage1> name=NC_008395:34917690..34921299 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008395:34917690..34921299 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atggcggccgctgcaggggcagcggcgcgcggtggcggtgtgatcccggccggtaagggtggcagccttcggtcgccggggaaacccgtcgtgctcgccgacatcaccaacaccgggaggcccaaccctactggatccgtccacgccatcgctgacgtcctcaaggagaacgccaagttgcgtcatctgcttgctgagcgaaacaaagtcattgaagtcagcagagtagagttgcagaaaatccgcctcgcactgcaagccatgcagcagaagaacttgcaactcgtgcaggctaattcgcagatgtttgcggtttgtctacttatccattag</cdnaseq>
Protein Sequence	<aaseq>MAAAAGAAARGGGVIPAGKGGSLRSPGKPVVLADITNTGRPNPT GSVHAIADVLKENAKLRHLLAERNKVIEVSRVELQKIRLALQAMQQKNLQLVQANSQM FAVCLLIH</aaseq>
Gene Sequence	<dnaseqindica>699..828#557..594#260..408#152..167#tctccgaaacctcatcggattcctctccgcgcggctaccggagcgccgcctctctctccccgcgcgcgcgctgcggctccgagggtcctcgccggcttcacctccggcggtggcgtgcgcaaccacaggccccgcggccgctgggccagccatggcggccgctgcaggtaaaaatcgtatcccctagatttcgagctacccgtggcattccatggtggggttctctcgggctcagcttccgcacctcctcctaaaccaggggcagcggcgcgcggtggcggtgtgatcccggccggtaagggtggcagccttcggtcgccggggaaacccgtcgtgctcgccgacatcaccaacaccgggaggcccaaccctactggatccgtccacgccatcgctgacgtcctcaaggtgatttcagttctagctctattttttttttcttcgggggatttcggttctaactcaaacaagcagacaaccctagcagcgccaagttgaatgctagtaattccgatttccatccgattaaaccatttaattcctcccttgctttcaggagaacgccaagttgcgtcatctgcttgctgagcgaaagtatgcattgcctctaccatccattttgttgtcggaggataaaacctgcggtttctcaagtagcacagttttttttaaattattttttgctttcttatttgcagcaaagtcattgaagtcagcagagtagagttgcagaaaatccgcctcgcactgcaagccatgcagcagaagaacttgcaactcgtgcaggctaattcgcagatgtttgcggtttgtctacttatccattagctccctacctcaccattcttgtgtcaatgtttgtttatacttgtgcaatgctggttcaccaggcctgtaggttgagcacatagtttgagttatagctatgcataatggcaaatgagaagtgtttgtcttacttcattgctgattgttttgtggtagttacagactgtttgatatgtctatgttttcttcttccttgcaggaaataaatcaaggaaaagatagagtaagctgcctctggttctttgttgtttgaattttcaaatgcagtatgatttctgatttggggattaatctgtggatatgacatgcagatcaagttattgcaacatgaacttgcatgcacaatagccgtgctcaaagtaaagggttctgaactcgaggtaattctaaagcttgactgtatgaacctctgtttgttacatataacatgcttttctcatcgtctactattgcagaagatgagtaagacttctaacaatcaacaaaatagagcaaagatattggtatgcagtcaaattcttaagcaaatgattcaccatgtttagctgcattatttctagttctgaattctgaaatcgttctgttcgtgcaacataatcaggagaaaaaaaccaggtcttccaaatgcgcaccaactaaggctcatcaaatggctgcaggttctattagagaacatttggttgaaattcaatgtaatgctcactcttctcactatttctttcttgaactagatactatacctggcaatcgtttcatctatcctactttatggttataatttaccccctttatatgaagcaattgaagttttatgttttttcctctgccataagttaggacactagtaaaattctaatcttcatttgttgccaatttcttctcagcagctgtgccatcttatactagctgtcatgagcctccacaggataaaacaaacaagaggtattttcatctctttactgatgtttgagtgggtgactggttgaccgggttatagtagcctatttcatgcatcactacttattcagcatgtttgcaaacaggtgcacaaataggcggaagtcagaatcatgtgaagttacaatggataccaacacagtgcaacatagctgcagacctcatgtggaatataatgggtcatcgcatgatgatgatccaaggtaatcaatgcttaatgcttataacagaatgcgttcattgttgcatcattatctgatttaagcttgttcaaaactcttgggcacatgtggtcagaaaaactcgacggagaaggtctgccagattgaaccctggatcttttgaggtcgcagagatctgtgataaattgcatgaggatgctactgttccttcggctcctagttctaatgttccaaagctgcaggaaccaaatgctggaaaagatatggtagggttttactatttctttctgtttttctgtttcaaaaaaaatttcacaatgtggttatgattttgtgctagatttgtggtggcaagatgaagtcattgcaaaaagaacttccatgtgatgcaatagcgcaagtagtagaggctccagaactcaaggtaattgagcagagtggttcgcatcaaatttttgctagtcttacatatattaatttattttttagtaaaatctggatcctgattgtatgtactagaaaaaacgaagtatgcacacacaaaaggaagtgtatctggaggtgggcaggacagttcttatgtaaataatcctcacatttatccatgaagaagtcttaattcttattatgttcctacttctttcgtccaatgaaattaggagatacaagaagcaggttccagcgttgctggaggtgaggcgcataaatttgatattgaggatccagagccaccaaggtatggcacatcattttagcagggtttaagatgcatggtacatcacacaattcacctaaacaatatgctctgtccaatcagaaaatcaatgcgtattgatgcaaacaaacggaagctggaatcatttgagagtcgattggcctcgaacaaagaggactgcatcaatgccatatgcgactcaacttcaagcgtgccaattcagcatgaacaaaagaggtaactctctcctatattccaacgatgcactttcctctgcatggaacatgtcaatcatggctgcccttaatactgcagaaaattgtcaaggagaaaatcctcaagactggaccctggaccttgggaggtcacaaatggcacttttgaaattgtccaggaagatacagttgccccgtctgctccttccagttcaaatgctttgatcgagcagaccaaaaatgatatgcaaaacgaccgcagttgctcgactaaaccttctgacgagcaggtgataggtagaagatcttcagttgggaggccctcaagacgggcagcagaaaagatagtctcctacaaggaggtgcccttgaatattaagatgcgacgaccatgatccccacttgcatgcaaagagcacaaacaccattggcatttattttatggtgtagaatcaagtttgttgtgtatctatctgttttggtcgctgtcaataggtaggttagctcctctatctaccccaacatggatactcaccctgcatcccagtttcaaactgaccatggaatttatctgatttagcctcagtctggactgatgatgccgataacaaccagcaaaccattgttcctgttctgatgaagtagagaccagacaacaataatgtggttagtactgttttattttcttgatggttctcatatgttgagagatc</dnaseqindica>
External Link(s)	NCBI Gene:Os02g0799100, RefSeq:Os02g0799100