Os04g0452500

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Annotated Information

Function

Nucleotide sequence analysis of the ZEP1 cDNA revealed that it is comprised of 3391 bp with an open reading frame of 2610 bp. ZEP1 has 21 exons and 20 introns The ZEP1 protein contains 869 amino acid residues, with a central coiled-coil region of 650 residues (amino acids 64 to 713; The N-terminal globular domain of 63 residues (amino acids 1 to 63) is basic, which is different from the acidic N termini of the mouse and budding yeast TF proteins but consistent with the Arabidopsis protein. The C-terminal globular domain of 156 residues (amino acids 714 to 869) has a high pI (9.44) and putatively binds DNA［1］.Additionally, the C terminus of ZEP1 contains two S/TPXX motifs, SPET (amino acids 751 to 754) and SPIT (amino acids 767 to 770), which are possibly involved in DNA binding［2］.ZEP1 Is the TF Protein of SCs in Rice. ZEP1 is the central element of the synaptonemal complex. ZEP1 is reloaded onto chromosomes in early microspores as the chromosome decondense. ZEP1 might be important for maintenance of chromosome decondensation during early microspore development.

Expression

Wild type vs. Mutant The mutant is the Tos17-insertional zep1 mutants and four single Tos17-insertion mutant lines of the ZEP1 gene were identified by screening the public insertion line collections. Sequence analysis of their PCR products confirmed that Tos17 was inserted in exon 8 of two independent lines and in exon 12 and intron 12 of the other two lines, which were referred to as zep1-1, zep1-2, zep1-3, and zep1-4, respectively. The four homozygous zep1 mutants showed normal vegetative growth and development, but with decreased seed sets. The seed setting and pollen fertility of the ZEP1/zep1 heterozygote plants are normal(Figure 1). The zep1 mutant shows abnormal synaptic behaviors and more COs during prophase I. In zep1-1, the chromosome behavior was almost the same as in the wild type from leptotene to zygotene (Figures 2J and 2K). At early pachytene, the two threads of the bivalents were always visible, although the homologous chromosomes could align perfectly along the entire chromosome length (Figure 2L).However,during early prophase I of the wild type, the homologous chromosomes began to pair (Figures 2A and 2B). The SCs were fully assembled at pachytene (Figures 2C and 2D) and fell apart. At late pachytene, all of the chromosomes were separated into single threads, while the two homologous chromosomes of each bivalent were still connected by several chiasmata, indicating that COs had occurred during the early stages (Figure 1M). At diakinesis, 12 bivalents could be detected in every nucleus (Figure 1N), and the two homologous chromosomes of the bivalents were tightly connected side-by-side and ringshaped bivalents were seldom detected. At metaphase I, 12 bivalents in zep1-1 were lined up on the equatorial plate (Figure 1O), but the two centromeres of each bivalent were dragged out far away in opposite directions by spindle fibers. the chromosome behavior was almost the same as in the wild type at anaphase I to zygotene and during meiosis II. By means of RT-PCR analysis shows that ZEP1 isexpressed mainly in young panicles and roots, and to a lesserextent in leaves and stems (Figure 3A).

Evolution

ZEP1, a transverse filament (TF) protein, is the rice (Oryza sativa) homolog of Arabidopsis thaliana ZYP1. TF proteins have been reported in many organisms, including budding yeast, Drosophila melanogaster, Caenorhabditis elegans, mouse, and Arabidopsis thaliana［3］［4］［5］［6］［7］［8］［9］［10］.ZIP1 in budding yeast was the first TF protein identified［5］［11］. You can also add sub-section(s) at will.

Labs working on this gene

State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China

Structured Information

Gene Name	Os04g0452500
Description	Prefoldin domain containing protein
Version	NM_001059487.2 GI:297602830 GeneID:4336010
Length	8343 bp
Definition	Oryza sativa Japonica Group Os04g0452500, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 4
Location	Chromosome 4:22981985..22990327
Sequence Coding Region	22981985..22982141,22982236..22982285,22983717..22983924,22984349..22984447,22984609..22985285 ,22985363..22985611,22985688..22985816,22985901..22985969,22986063..22986173 ,22987241..22987348,22987421..22987531,22987605..22987691,22989003..22989092 ,22989175..22989267,22989395..22989484,22989579..22989641,22990229..22990327
Expression	GEO Profiles:Os04g0452500
Genome Context	<gbrowseImage1> name=NC_008397:22981985..22990327 source=RiceChromosome04 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008397:22981985..22990327 source=RiceChromosome04 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atgcagaagctgggtttatcggggcttaggggtctcgaggggttccgatctctcgctggatccacctcgacggccgcgaaggccccgaaccccaagccgtcgtccgatattggagggagcacgtacgggagcttcgccaatcttaagatcacagcagaaaagctggttaaggagcaggcttcagtgaagactgatctagaaatgacgcataccaagttgagaagagcaacagaacagataaatctgttagaagcaaagctccaacaagctgtcaatgaaaatgcaaagcttaaggtgaaacagactgaagattcaaagctctggcagggattagattcaaaagtttcctcaacaaagactttgtgcaatcaactgacagaaactctgcagcagttagctagtcagacagaaagagggaaacaggagatgttgcggatcaaacaagagaaagaagagatggatcagagttacaaggaacagctatatgcaagtgatactaccataagagaaaaaaattctctcatcaaacagttggaagattccattgaacaaaataaagcccgactgttgtatgttgactcccgtttggaatgcatggagcaagagttgaagctaaaagaagatgtttgcatttgtctgaaagaaaacctggcaagcactgaaagtgaaaagaatgacttgaagcttaggaacgagggctacactttggaagttcagaaactatccaaggacaacaaggagcttaatgaattgcttagtggttttacggttaaggtaactgagctagataaagagcacacatcaatttcaagtcatgtcactcagttgatttcttcatttgagagatatgatggaaaggttcacgaggaaaaaatgttgatgataaaatctgccaaggacaaatttgaacatctccaaaatcagtatgtaaatttgatatcagaaaacaatgctctccaaactgaaatcgaggagctgaagtccagaatcatagagctgcagaaaactcaagagattgtcatggttcaacatgtagaagaatgccaagttgctgaagataagatcagaagattggagtctgaagcagaaatttctgcctccaacattagtcagctagaaaaggtagcttctgacctagaagggagggttcaaaaacttctggaggattctagatctgctgaaaatcacaagcaagagttgcttcagaagattctgaagctagaatcagataatcaggagcttctaggtcgagtgcaatctgttttgaatgagaagtctaatgatacggaatctctgcaaggagagatagctaagcgtgaccaacaggttgagacacttgagaaccaggtcaatcagctttgcagtattattgatgagaaagaacaactacacacttgtgctgtagaaagagagaagaatttagaggaacagaagttacaggtccaagcatcacttgctgcaacagaaagccaacttactgaggcaaaaaaacagtatgatatcatgcttgaaggtaaaaagatagagctgtccaagcatctgaaagaattatcactgaaaaatgaccaggcaatcaatgaaatccgtaggaaatatgaactagaaaaggtagaaatcattaatattgaaaaagaaaaggccgagaagctcataaaggaaatggaaaacaaatgcaatgaaaagatttcagaaaacaggcaagattctgagaggtatttgatgtgtctaaaggaggagcatggctcaatggttgcaagaattcagcaggataatgagcataaggaatcaactcttcgagcttatcataaagaagaactccagcgcatccaatctcaggctgagaatgaattgagggagagattgtcatcgctcagaaaggatcatgaaattcaaatgaaatcattgactaaaaagcatgaggaaaattgtcagaagcttcaggatgaactggagcttcagaagtcaaaggaggagaagcaaagagcattgttacagttacaatggaaagtaatgggcgaaacccaacaggttgatcaagaagtaaactctaagaaggaatactctgtttcatcaataaagaggagagatccatatatcagaaaagaacatcagcttcagttggttagtcctgagaccaaacggaaggatgttaatctatctggcattatacaatcaccaattacaaacatgctgagaaaggtagagaaaggaacccaggatattcctaagcatagaaaggtaacacatcatgaatatgaagttgagacagcaaatggaagaatcacaaagcgcaggaaaactaagagcaccgtcatgtttggggtacaggaacccaacactcagaagtcattgcatgatactgctgacaaggatcctacaaaaatgaagaaggttgttgctggatcccatccccaccctgcaaacattggtgaattgttttctgagggctcgttgaatccatatgccgaagatccttatgcatttggctag</cdnaseq>
Protein Sequence	<aaseq>MQKLGLSGLRGLEGFRSLAGSTSTAAKAPNPKPSSDIGGSTYGS FANLKITAEKLVKEQASVKTDLEMTHTKLRRATEQINLLEAKLQQAVNENAKLKVKQT EDSKLWQGLDSKVSSTKTLCNQLTETLQQLASQTERGKQEMLRIKQEKEEMDQSYKEQ LYASDTTIREKNSLIKQLEDSIEQNKARLLYVDSRLECMEQELKLKEDVCICLKENLA STESEKNDLKLRNEGYTLEVQKLSKDNKELNELLSGFTVKVTELDKEHTSISSHVTQL ISSFERYDGKVHEEKMLMIKSAKDKFEHLQNQYVNLISENNALQTEIEELKSRIIELQ KTQEIVMVQHVEECQVAEDKIRRLESEAEISASNISQLEKVASDLEGRVQKLLEDSRS AENHKQELLQKILKLESDNQELLGRVQSVLNEKSNDTESLQGEIAKRDQQVETLENQV NQLCSIIDEKEQLHTCAVEREKNLEEQKLQVQASLAATESQLTEAKKQYDIMLEGKKI ELSKHLKELSLKNDQAINEIRRKYELEKVEIINIEKEKAEKLIKEMENKCNEKISENR QDSERYLMCLKEEHGSMVARIQQDNEHKESTLRAYHKEELQRIQSQAENELRERLSSL RKDHEIQMKSLTKKHEENCQKLQDELELQKSKEEKQRALLQLQWKVMGETQQVDQEVN SKKEYSVSSIKRRDPYIRKEHQLQLVSPETKRKDVNLSGIIQSPITNMLRKVEKGTQD IPKHRKVTHHEYEVETANGRITKRRKTKSTVMFGVQEPNTQKSLHDTADKDPTKMKKV VAGSHPHPANIGELFSEGSLNPYAEDPYAFG</aaseq>
Gene Sequence	<dnaseqindica>1..157#252..301#1733..1940#2365..2463#2625..3301#3379..3627#3704..3832#3917..3985#4079..4189#5257..5364#5437..5547#5621..5707#7019..7108#7191..7283#7411..7500#7595..7657#8245..8343#atgcagaagctgggtttatcggggcttaggggtctcgaggggttccgatctctcgctggatccacctcgacggccgcgaaggccccgaaccccaagccgtcgtccgatattggagggagcacgtacgggagcttcgccaatcttaagatcacagcaggttttggtttcattttccccttgtttccttagagggaggctggctacttcgtgcgttcactgatttttccctcaattttattgcttgatcacagaaaagctggttaaggagcaggcttcagtgaagactgatctagaaatgacggtatatttttcagcgtttaccattatttatggaaattttactggatggggtatatagttcggttgtcttgtgctcctgtcacagtttcgatttctggatattaggatatatctggcactgcctttgtggaattgtggttataatgcctaatagcagaaaaactgtcttctgattgatcaatgtcaaacttgcaaagttgtttatgttagtagggctgtttcagttatataatcctggcggcatgatacatacataggcaggggtgtacctatgtgtagtctacaggggtcctaggaccccgggtagattatctaattctagctaatttatgtcatgataattagtataatcggtaaataattaaataattagtatgtttgagaccccggtaaattttgttttggattctccactgtaacataggggtagttttctattcccaattctgtcagggaatcctatcatgattttgtggactactgaaaccagcagtaggtaaaaccaatgaactgtgcacacacctggagctaccttattagctttgaatcgagtaactggtaactatgcacgcatgccagttaccaacatgtcaataaaaatcttctaccattgtaggcagttcacaagcatcaaattcttactccctccgttttttaatatatgacgccgttgacttttgttacaacctttgaccgtttgtcttattcaaaaattttatacaaatcttaaaaatattagtcatgcttaaagttcatttagtgataaatcaaaccacgacaaaagaaaatatatttacttaaatattttgaataagacgaatggtcaaacatgtgagaaaaagtcaatggcgtcatctattaaaaaacggaggtagtataattttagggctgcatgtagatagtgtggatctcatgactgtactttgtgtttgttattcctattactttcctcagaatttacaaatacacagtcgtgctgaaaattgaaacagttttctacttaaaggatgttggatgctgtatatcatcagttttcactattcagcatgacagagtcacattctggaattatgtatttttataaatttgaactttgtacctggaaaaactagaatgttttggtgctttatcctctgtggtttctgttaaacacattggagatcaagcaatttctgccttccatttcatttgtcccatatcctgagctttgaacataagcttgcgcccttccatgttcagtatatggcaatattatctttactgtagtcatctttcatggtgcaaatgttctagttatatcatggataaaaggtaaattgactcaagtcctgaacagatatatcaccatatatcatcgttttgtacaggaacatattgtacttgtttacataatatttcttactactgatgcctatgctctatataactttagcataccaagttgagaagagcaacagaacagataaatctgttagaagcaaagctccaacaagctgtcaatgaaaatgcaaagcttaaggtgaaacagactgaagattcaaagctctggcagggattagattcaaaagtttcctcaacaaagactttgtgcaatcaactgacagaaactctgcagcagttagctagtcagacagaaagaggtaatgtttccatttactcatgcatgagtgctgtatagatctaactggtgaaacaacgcattggcagctgaggaagataagaagttttttgaggagattcttggcaagaattcaaaagcttttgaagaattcaactgcctgttgcacgattcatcaataaaactggaatgtgcagaacaaatgatcatttcaggttggtcagtttcttcacgcgtcagctctagcactagtattttagaatgtctgtcaattcagtaacttgctactggagactgtagatctgctaggggatcactatctgctgtgcttctgtttatgtcatctaaaccatgattattttgcagttccatgtttcttgacatgatttgtgaataataatgtgctgcaattaggtagtaatgcttcatttgtttcattgacacagggaaacaggagatgttgcggatcaaacaagagaaagaagagatggatcagagttacaaggaacagctatatgcaagtgatactaccataagagaaaaaagtatgaatctaatactaaaaattgcatcatgactaaacagaaattaagttagctatggtgcgatagtccactgaaaattttttgtatactccattcatgtgcatccggtgtattagcacaaacaaaaagtagtaacacaactgtgtccatcttttatgcagattctctcatcaaacagttggaagattccattgaacaaaataaagcccgactgttgtatgttgactcccgtttggaatgcatggagcaagagttgaagctaaaagaagatgtttgcatttgtctgaaagaaaacctggcaagcactgaaagtgaaaagaatgacttgaagcttaggaacgagggctacactttggaagttcagaaactatccaaggacaacaaggagcttaatgaattgcttagtggttttacggttaaggtaactgagctagataaagagcacacatcaatttcaagtcatgtcactcagttgatttcttcatttgagagatatgatggaaaggttcacgaggaaaaaatgttgatgataaaatctgccaaggacaaatttgaacatctccaaaatcagtatgtaaatttgatatcagaaaacaatgctctccaaactgaaatcgaggagctgaagtccagaatcatagagctgcagaaaactcaagagattgtcatggttcaacatgtagaagaatgccaagttgctgaagataagatcagaagattggagtctgaagcagaaatttctgcctccaacattagtcagctagaaaaggtagcttctgacctagaagggagggttcaaaaacttctggaggattctagatctgctgaaaatcacaaggtttgtgtcctagcctgttcagtgacaaaacatattgctatttaacctgtaagattcatgaggtttgaattttgcagcaagagttgcttcagaagattctgaagctagaatcagataatcaggagcttctaggtcgagtgcaatctgttttgaatgagaagtctaatgatacggaatctctgcaaggagagatagctaagcgtgaccaacaggttgagacacttgagaaccaggtcaatcagctttgcagtattattgatgagaaagaacaactacacacttgtgctgtagaaagagagaagaatttagaggaacagaagttacaggtctgtgcttattagaactttaaatctcattgtaatgatctatagacaacatacaatcttttctgtctactgccaggtccaagcatcacttgctgcaacagaaagccaacttactgaggcaaaaaaacagtatgatatcatgcttgaaggtaaaaagatagagctgtccaagcatctgaaagaattatcactgaaaaatgaccaggtattacgcttctatcagctgtatcctgtatgacactttgctatatcaatcacaagtgacagttagaaaagtgcatttttgcaggcaatcaatgaaatccgtaggaaatatgaactagaaaaggtagaaatcattaatattgaaaaagaaaaggtaagttaacctcttttttcaaagttattatgctagctgttttgagtttgatcttattctctatctattttctggatgttgaaaaatatttaggccgagaagctcataaaggaaatggaaaacaaatgcaatgaaaagatttcagaaaacaggcaagattctgagaggtatttgatgtgtctaaaggaggagcatggctcaatggtaggtggtactggttggatctgtccattaaagttaagctgttttgaaaaaaaaaagtgaagtttacaattagttatgataacaatgctaattttgaaatttgaaaatgattattaataattattttaggttgaagtttgtttatttgtcttggtagcaatcatgaaaagagagctaggcaataaaagtgaataaaaacctttaaaattaatgctaaaatcaaatcctaaaattgaaaaaagctgcagtttttaagggaaaaaactgagccaagtggagccacatgcgccagtttccattggaaatgttagctctagtatttgaacaaggtgttgatagcatggatacataattgcattgtggatgttcatgttctgctatgctgcattccgctacagaggattagtaacctaaaaaggttactcaaggtctttctacgtgtgcttttaatatgctttgtccaaaacacccacttggtagttatatgtgttgcaaagtttctttgaaggtgctttaggtaatgctctccttgcctgtcttttatttaatttgcactaggcacaaaacatgacatcaaagtatgatctatcatatttcatcaatatacattgttaatgtaaatggtatttggactttactggttgatttcatggttctcttaagatttaataagtcaaaaatgtcatctgatatttgagcagagctgcagagaatgtatttgacacaactttatggccgtaggacttacacatctattctaatataattttgatgttccatttggttttgcagcagttttgagatggtatatttttttacataaagtaaaaggggtgcagtgaaaagtctctgtaaaaatatgttcctgtaaattttgagagtcaaactttcatcactatctgtagaatcctgccactaatgaatttccatttgttgaagattgaaactattgtctaaaggatcccacctctggagtggggaagcactgaacactacctcagcggaaagtctgaaattagactgtttttcttctaaaatgaccatgaaagttgtttcttgatatttaggttgcaagaattcagcaggataatgagcataaggaatcaactcttcgagcttatcataaagaagaactccagcgcatccaatctcaggctgagaatgaattgagggaggtcagtaataaattccaccagatgttttgatgatgattgttcatactaattaaatctggatcaaatatgcagagattgtcatcgctcagaaaggatcatgaaattcaaatgaaatcattgactaaaaagcatgaggaaaattgtcagaagcttcaggatgaactggagcttcagaagtcaaaggtagtcactctggtgatcttcttgttctggaaatcgttacttgaaaatatatctgaacacttttttttttcaggaggagaagcaaagagcattgttacagttacaatggaaagtaatgggcgaaacccaacaggttgatcaagaagtaaactctaagaaggtattctctgatttctatctcatatactatttttaagcaattcatatttaagttatgttatattaattgcatagcatgctaagcatttaatataaagatgcaagtgcatatgtttcactaattgaaatgctgctgcttatttcatgtaacaataaattcaagaaattgagtattttgacttggtttggcattaatttataaagccttgtggcttgtatttcgaatatgtaggtcaagacaattggttttttttccaggtttgacattttctaccacaaccattatttccttgtggattgttattaagaaaaattagatgaagattaggaatgagtcatttggttgatttgattggataaattaatggaaagtaaaggataagattcattttccatgctttttaattctttatttgatattgtcaatatattcctaaaccatacataatagagagattttaatccctatgtggtaattatgaaaacattctgttttaactttcgagttggctgttgatgcatgcaaaactttttaacataatcaagtttgttccttactctcatgtatatatatatatatatatatgactcctaactcagatatacatgactgtggggagtacttctaaaaaaactactattgtgtcaaatttctgtgcacttagttatcttttaacacatcacactttgctttccttttttattatgagcagaaatagtatttgtttcagactttatgttgtagattttgatgcgccattttggccttttatttttcattacaatcctattatttatttcatgccttgcaaactctgaacttgctgtgtttgcatataggttcctgtgtgtgcgatttggttcatctgtagaggtgctagttgcaaactagtgaaatactattgacttttttttttcacgatcttgaccatagatttgttcaacccctaagtcccagtatgacactttttgataagaataaatgaaagaaaaaacatttttcttcacctgtttggattataataccagatgttcatacatctcattcttgctagattttcccaaagagctccttaaacatatcttacaaattcaggtaaaaatggaaaatatctttaaaaatggttaccatgatttggtcacgtgctcatgttggggcagaaccagaaaggctgatatagtccatatcatgcattattttcagtttttgttaatcttataattggttctttgtaatttgtttcaccgtttacgttttgtatgtgccaatgctctccttaagagttaagacatacatattttatttttttaggaatactctgtttcatcaataaagaggagagatccatatatcagaaaagaacatcagcttcagttggttagtcctgagaccaaacggaaggtaagacatctctctttaaacgttgactttatcactgcatgcttcattgcacaaattctaaattcgcatgttctactaccaggatgttaatctatctggcattatacaatcaccaattacaaacatgctgagaaaggtagagaaaggaacccaggatattcctaagcatagaaaggtgaatcattttacatttagattcatcattctcttattagagaatgtatcaagtttcactaattgaatgaattaatctgtaaaatacatgttttcatgtgacatgttttcggtgtccttttaactaggtaacacatcatgaatatgaagttgagacagcaaatggaagaatcacaaagcgcaggaaaactaagagcaccgtcatgtttggggtacaggtttttcctctcttttttctcctttgcatttgattggtggtctagtatttcattcttgtaatcatgttaacagtagcctttttctcccattcaggaacccaacactcagaagtcattgcatgatactgctgacaaggatcctacaaaaatgaagaaggtatatttgtgtgattaaatgtgttcgccaacttctttccatatgcatgtctaaactactccctccgtttcataatgtaagtcattctagcattgcctatattcatatagatgttaatgaatctagacatagacatatatctagattcattaacatccatatgaatgtgagcattgctagaatgacttacattatgaaacggaggaagtagattcattgacctctgtaattttgttttttgtttttgggcatatagggatacttttatagaattgaaatattttctgtgtactatatcattttctgaattatgatctgtactatgtgatatattcaggaatgcctgttctcatagatgtttctgttgtaagaacatttgtagttgacttactactttgatggtacagcatacatttattagatagtgttgactcagttttattctctcgctaaatgactgaaaatttgtgattgctcatgtggtggaattcgatcagagtctatccgaaaacatgcatccagaatattggttttcccagatgcttttgctcaacttttgttaacatttacgcttcttcactctccaggttgttgctggatcccatccccaccctgcaaacattggtgaattgttttctgagggctcgttgaatccatatgccgaagatccttatgcatttggctag</dnaseqindica>
External Link(s)	NCBI Gene:Os04g0452500, RefSeq:Os04g0452500