Os11g0117400

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Annotated Information

Function

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WRKY genes are a family of transcriptional regulatory factors that have specific functions in plants.Plant WRKY gene-encoded transcriptional regulators appear to be involved in various physiological programs, including disease-resistance,senescence,stress responses of biotic and abiotic, growth and development processes.

Expression

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Expression of OsW89p::GUS was observed in leaves, stems, and sheaths; pollens hadn't expression; mature seeds hadn't expression; the embryos of germinating seeds had expression; primary roots, adventitious roots and their lateral roots had expression but not in root tips of seedling stage; only lateral roots had expressin in roots of maturation phase.

Analyzing inducible expression pattern in T1 transgenic seedlings, the results showed: expression level of OsW89p::GUS can be increased by MeJA, IAA, ultraviolet, high temperature, low temperature and wounding treatment, inducible effects of MeJA and ultraviolet were distinct; and can be restrined by 2, 4-D; ABA and SA can't influence OsW89p::GUS expession; NaCl and PEG can restrain expression level of OsW89p::GUS in roots but increase expression level in leaves.GUS activity of OsW89p::GUS was higher than 35s::GUS in trangenic plants.In conclusion,we thought OsWRKY89 gene promoter was an inducible promoter with tissue specific expression characteristic.

RNA gel blot analysis indicated that OsWRK Y89 was strongly induced by treatments of methyl jasmonate and UV-B radiation. The transient expression analysis of the OsWRKY89–eGFP reporter in onion epidermal cells revealed that OsWR KY89 was targeted to nuclei. Transcriptional activity assays of OsWRKY89 and its mutants fused with a GAL4 DNA binding domain indicated that the 67 C-terminal amino acids were required for the transcrip tional activation and that the leucine zipper region at the N-term inus enhanced its transcriptional activity. Overexpression of OsWRKY89 led to growth retardation at the early stage and reduction of internode length. Scanning electron microscopy revealed an increase in wax deposition on leaf surfaces of the OsWRKY89 overexpression lines and a decrease in wax loading in the RNAi-me diated OsW RKY89 suppression lines. Moreover, extractable and cell-wall-bound phenolic compounds were decreased in the overexpressor lines, but its SA levels were increased. Lignin staining showed an increase in lignification inculms of the overexpressor lines. Intere stingly, overexpression of the OsWRKY89 gene enhanced resistance to the rice blast fungus and white-backed planthop per as well as tolerance to UV-B irradia-tion. These results suggest that OsW RKY89 plays an important role in response to biotic and abiotic stresses.

The inducible expression characteristic of OsW89p in rice detected by GUS fluorometric measurement revealed that:1)Expression level of GUS was increased 4-fold by MeJA treatment, and repressed by the presence of 2, 4-D, but was not influenced by salicylic acid; 2)The expression of GUS was repressed in the roots and promoted in the leaves by NaCl and PEG;3)Ultraviolet, high temperature(42°C), low temperature(5°C)and injury treatments can increase the expression of GUS leaves, and the induced efficiency of ultraviolet was the highest.

Evolution

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Accordingly, the rice genome has been pre-dicted to contain 1,611 transcription factors. However the genome of single-celled yeast Saccharomyces cerevi-sae contains only 12% of these genes. Thereby, indi-cating that evolution from primitive to complex life forms involves an increase or expansion in regulator genes. Sequence specific DNA-binding transcription regulators act in concert with other components of the transcriptional machinery to modulate the expression of specific target genes in temporal and spatial manner, necessary for normal development and proper response to physiological or environmental stimuli. Transcription factors are identified and classified according to their DNA-binding domains (DBDs) and therefore, the names of the DBDs, e.g., AP2/ERF (or EREBP), WRKY, NAC, are also used as thenames of the transcription factor families.

Knowledge Extension

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WRKY genes encode transcription factors that are involved in the regulation of various biological processes. These zinc-finger proteins, especially those members mediating stress responses, are uniquely expanded in plants.

The WRKY gene family, important for plant development and responses to both biotic and abiotic stresses, has encoun-tered several duplication and deletion events in very recent evolutionary history in Oryza sativa. Completing the annotation of this family in rice will enable the determination of whether these changes are simply neutral occurrences in a heavily redundant family of transcription factors or whether they ac-tively contributed to the variation between modern rice sub-species and cultivars. Mounting evidence indicates that WRKY genes modulate the signaling networks for all hormones and regulate the biosynthesis of starch, sesquiterpene, and alka-loids in a variety of plants, including important crops. There is no doubt that studies of this family of transcription factors will not only further our understanding of the fundamental pro-cesses that are controlled by WRKY genes, but also help en-hance agricultural productivity.

Labs working on this gene

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Department of Plant Pathology, State Key Laboratory of Agrobiotechnology, China Agricultural University, Yuanmenyuan West Rd. 2, Beijing 100094, China

Biotechnology Institute, Zhejiang University, Hangzhou 310029, China

Department of Biological Sciences, Hunan University of Science and Technology, Xiangtan 411201, China

Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China

Bioinformatics Core, School of Life Sciences, University of Nevada, Las Vegas, Nevada 89154, USA

College of Agriculture and Biotechnology Zhejiang University, Hangzhou, P. R. China May. 2005

Structured Information

Gene Name	Os11g0117400
Description	DNA-binding WRKY domain containing protein
Version	NM_001072123.2 GI:297611109 GeneID:4349611
Length	1015 bp
Definition	Oryza sativa Japonica Group Os11g0117400, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 11
Location	Chromosome 11:781474..782488
Sequence Coding Region	781474..781891,781997..782104,782198..782463
Expression	GEO Profiles:Os11g0117400
Genome Context	<gbrowseImage1> name=NC_008404:781474..782488 source=RiceChromosome11 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008404:781474..782488 source=RiceChromosome11 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atgatagagcaccaaaaggctctcatggtggagctgcgtggcatggtcatgccattacttccgagcgacaatgaacaagccaagcttgctcttcaactcttgggagatatattgagttgctcagataaggctatctccatgctagaacttggtggagacactaaaaagctgaccaatcttgttggaggtaagagaaaaggtgacaagcatagcatggacaaccacaacttggaggaggaagccaaagaaagtgttagcaagagaagaaagaatgcagaacacacaggttcaactgtggctcaagcacctcacaatgatggacatcaatggaggaagtatggtcagaaatggatctctagagcaaaacattccaggagctactataggtgtgccaatagtaaagtgcaaggctgtcctgctacaaagacagtgcaacaaatggattccagtggaaatggaacatcaaagttgttcaacgttgactactatggccaacacacatgcaggggagatggcatagccgatccatatgttgtcgacacagcccatcacagtatggaacctatcaatcaaaacgaatgcaatagccctacacttgaacacgaagcccatgaagttcaagatgaaagatttgaaaacttgtgtatggtacaaaatatgccagagtatttgatagattttgaattggagagagccttcgagtttattgtgaactcaccattgggttctgagcattggacgttcgatgattcaataagatgtgagcacagtccaatatgcatatggggatga</cdnaseq>
Protein Sequence	<aaseq>MIEHQKALMVELRGMVMPLLPSDNEQAKLALQLLGDILSCSDKA ISMLELGGDTKKLTNLVGGKRKGDKHSMDNHNLEEEAKESVSKRRKNAEHTGSTVAQA PHNDGHQWRKYGQKWISRAKHSRSYYRCANSKVQGCPATKTVQQMDSSGNGTSKLFNV DYYGQHTCRGDGIADPYVVDTAHHSMEPINQNECNSPTLEHEAHEVQDERFENLCMVQ NMPEYLIDFELERAFEFIVNSPLGSEHWTFDDSIRCEHSPICIWG</aaseq>
Gene Sequence	<dnaseqindica>598..1015#385..492#26..291#tagtgactgtcaagtagtgatcaacatgatagagcaccaaaaggctctcatggtggagctgcgtggcatggtcatgccattacttccgagcgacaatgaacaagccaagcttgctcttcaactcttgggagatatattgagttgctcagataaggctatctccatgctagaacttggtggagacactaaaaagctgaccaatcttgttggaggtaagagaaaaggtgacaagcatagcatggacaaccacaacttggaggaggaagccaaagaaagtgttagcaagagaaggtatatgtatataaatacatgccactagaggaatgtccactagcttatcattcattgctaacgaaatttgattgatcctcttgattatgatagaaagaatgcagaacacacaggttcaactgtggctcaagcacctcacaatgatggacatcaatggaggaagtatggtcagaaatggatctctagagcaaaacattccaggtataaatgagcatatgaactcctgtttatttccattcctcgaagcatattggttttatctaacagcaaaatatatacatctaatgagctcatttgcttaagcaggagctactataggtgtgccaatagtaaagtgcaaggctgtcctgctacaaagacagtgcaacaaatggattccagtggaaatggaacatcaaagttgttcaacgttgactactatggccaacacacatgcaggggagatggcatagccgatccatatgttgtcgacacagcccatcacagtatggaacctatcaatcaaaacgaatgcaatagccctacacttgaacacgaagcccatgaagttcaagatgaaagatttgaaaacttgtgtatggtacaaaatatgccagagtatttgatagattttgaattggagagagccttcgagtttattgtgaactcaccattgggttctgagcattggacgttcgatgattcaataagatgtgagcacagtccaatatgcatatggggatga</dnaseqindica>
External Link(s)	NCBI Gene:Os11g0117400, RefSeq:Os11g0117400