Difference between revisions of "Os04g0677500"
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==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | *Institute of microbiology ,Chinese academy of sciences. | |
| + | *International Rice Research Institute, Manila, DAPO Box 7777, Philippines | ||
==References== | ==References== | ||
Revision as of 09:12, 31 May 2014
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Contents
Annotated Information
Function
this gene named Os04g0677500 is located in rice CHR 4:35005101-35008954,which the length of this gene is 3854bp.the expression of this gene is Rice mitochondrial Pyruvate kinase [1]. Pyruvate kinase (PK; ATP:pyruvate 2-O-phosphotranferase, EC 2.7.1.40) is a key regulatory enzyme of the glycolyticpathway. It catalyzes the final step of glycolysis, converting ADP and phosphoenolpyruvate (PEP) toATP and pyruvate by essentially irreversible transphosphorylation.This enzyme is critical for the control of the metabolicflux through the glycolytic pathway. Moreover, its substrate (PEP) and product (pyruvate) are both involved in a variety of metabolic processes. Thus, PK plays a key role in the cellular metabolic flux [2].
Expression
using bioinformatics methods to scan rice genome, we get 10 with PK extremely conservative regions (PK - PK - C) gene.Os04g0677500 is one of that Homologous genes
,and it was highly expressed and composed of sexual expression [1].OsPK1 was expressed mainly in leaf mesophyll cells, phloem companion cells in
stems, and cortical parenchyma cells in roots[2]. the Representative sequence Os04g0677500 is AK100393
this gene expressed in different Organs and tissues of rice , and their Feature Number is 9888 21058 422901. their expression as following pictures show.
.
Evolution
a single T-DNA was inserted in the transcriptional regulatory region of OsPK1 in ospk1. Quantitative RT-PCR result showed that OsPK1 expression was decreased by approximately 90% in ospk1 compared with that in WT [2].
Labs working on this gene
- Institute of microbiology ,Chinese academy of sciences.
- International Rice Research Institute, Manila, DAPO Box 7777, Philippines
References
Please input cited references here.
Structured Information
| Gene Name |
Os04g0677500 |
|---|---|
| Description |
Pyruvate kinase (EC 2.7.1.40) |
| Version |
NM_001060800.1 GI:115461329 GeneID:4337406 |
| Length |
3615 bp |
| Definition |
Oryza sativa Japonica Group Os04g0677500, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 4:35005323..35008937 |
| Sequence Coding Region |
35005655..35006467,35007045..35007506,35008380..35008640 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008397:35005323..35008937 source=RiceChromosome04 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008397:35005323..35008937 source=RiceChromosome04 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggcgaacatcgacatggggaagatcctggcggggctggagaacgacgacgcgcgggtgcccaagaccaagctggtctgcacgctcggcccggcctcccgctccgtccccatgctcgagaagctgctccgcgccgggatgaacgtcgcgcgcttcaacttctcccacggcacccacgagtaccaccaggagaccctcgacaacctccgccaggccatgcacaacaccggcgtcctctgcgccgtcatgctcgataccaagggtcctgagattcgtactggatttttgaaggatggcaagccaatcaagctaacaaagggtcaagaactcactgttaccaccgattatgagatcaagggtgatgagaacatgattaccatgagttacaagaaactgccagttgatgtgaagcctggaaatgtcattctctgcgccgatggtacaatctctttgactgttttgtcctgtgatccaaaggctggaactgtgaggtgtaggtgtgagaacacagcaatgcttggcgagagaaagaattgcaatctgccaggaattgttgtggaccttcctacactgactgagaaggataaagaagacattttgggatggggtgtgccaaatgacatagacatgattgctctgtcgtttgtccgtaaaggatcagatttggttaccgtcagacaacttcttggacagcatgcaaagcgcatcaagctgatgtcaaaggttgaaaaccaagagggtgttgtaaacttcgatgagatcttgagggaaacggatgcatttatggttgctagaggtgatcttggaatggagattccagttgagaagatattccttgcacagaagatgatgatttacaagtgcaaccttgctggaaagcctgttgtgactgctactcagatgcttgagtcgatgatcaaatcaccacgtccaactcgtgctgaggcaactgacgttgcaaatgcagttcttgatggaactgactgcgtcatgcttagtggagagagtgctgctggagcataccctgaagtagctgtgaagatcatggcacgtatatgtgttgaggcagagtcttcccttgacaacgaagctgtcttcaaggagatgatcaggtctgcgccccttccgatgagcccattggagtctctcgcatcctctgctgtacgcacagccaacaaggccaaggcagccctgattgttgtcttgactcgtggtggtaccacggcaaagctggttgccaagtatcgtcccagggttccaatcctctctgtggttgtccccgtgttgacaaccgattcattcgactggacaatcagctcggagggcccagcaaggcacagcctaatctacagaggtcttgttcctctcctggctgagggttctgccaaagccaccgattcggagtcgacagaggtcatccttgatgctgctctcaagtcagctgtacagaagcagttgtgcaagcctggtgatgctgttgttgctctgcaccgtattggcgtcgcatccgtgatcaagatctgcatcgtgaagtaa</cdnaseq> |
| Protein Sequence |
<aaseq>MANIDMGKILAGLENDDARVPKTKLVCTLGPASRSVPMLEKLLR AGMNVARFNFSHGTHEYHQETLDNLRQAMHNTGVLCAVMLDTKGPEIRTGFLKDGKPI KLTKGQELTVTTDYEIKGDENMITMSYKKLPVDVKPGNVILCADGTISLTVLSCDPKA GTVRCRCENTAMLGERKNCNLPGIVVDLPTLTEKDKEDILGWGVPNDIDMIALSFVRK GSDLVTVRQLLGQHAKRIKLMSKVENQEGVVNFDEILRETDAFMVARGDLGMEIPVEK IFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSG ESAAGAYPEVAVKIMARICVEAESSLDNEAVFKEMIRSAPLPMSPLESLASSAVRTAN KAKAALIVVLTRGGTTAKLVAKYRPRVPILSVVVPVLTTDSFDWTISSEGPARHSLIY RGLVPLLAEGSAKATDSESTEVILDAALKSAVQKQLCKPGDAVVALHRIGVASVIKIC IVK</aaseq> |
| Gene Sequence |
<dnaseqindica>2471..3283#1432..1893#298..558#acctcgaccctctgcccctcaactcggtggtgaccacgcgtccacatctctcatctttctttctccgctattctcctccagtcctcttctcttcttcctccctcccttcgcccactgaatcgccaaaaaaattcccctcctccactccatctctataaaaatcctcccccaaatctcgcctcgtttagccagcacaactcacacaactctctctctctctctctctctctctctctcgtcgtctgcaagcttccgcctcccggatcgcggcgaccaccggaggaggagggagcagccatggcgaacatcgacatggggaagatcctggcggggctggagaacgacgacgcgcgggtgcccaagaccaagctggtctgcacgctcggcccggcctcccgctccgtccccatgctcgagaagctgctccgcgccgggatgaacgtcgcgcgcttcaacttctcccacggcacccacgagtaccaccaggagaccctcgacaacctccgccaggccatgcacaacaccggcgtcctctgcgccgtcatgctcgataccaaggttaccccctcactgctttgcttgtcgatctctacatgcttgtatgctgctgggttgttcggatcggaccagtcggggtagcttgcgtttgatacagagcggatttcgatttggatctgctcgtcttttttatcgggagaagctgggtttgatttggaggcagcatggtaggattatgagttgagtttacagcggtttgactttggaggactcgacaaaatggtatccggggttagatttgcctgcgcttgcgcttttgccttgtcgcagatggctggatcttggattgggggctcaggcttgcgtctccatgtgggtagttagttttggagtcttatggattctgagcaagtgtaagtgtgtaactggttcgtagtgtgatccgtttgcggtgttggtgagattgatgagctgcgtgcatcttgtttgtcatatttagctttacagttcgtctctatgctttttcaagttaatttattcatttgtagagttggattggaaggatggttaacagatcttgggatcggtggctttggagtatggagtgttcaaatcctagtgttctgattttgttgcttttgtaggaggatatgattaacacgtaaaagaaggctttattagtgtagtcatttcttctattatgccttgatgtttctgtcatgcacgtaactattggtgcttatctttttacactctcacacttcatggtttgctttgtagttcaaataagttacttacagaattgtactgcacacagtttatcttttttatcacttcttgtactgcaattagttccattatttttacctgtttagtagcatatttaaggcgattctgtggtgactgttctgaacatttcattcattgcttcagggtcctgagattcgtactggatttttgaaggatggcaagccaatcaagctaacaaagggtcaagaactcactgttaccaccgattatgagatcaagggtgatgagaacatgattaccatgagttacaagaaactgccagttgatgtgaagcctggaaatgtcattctctgcgccgatggtacaatctctttgactgttttgtcctgtgatccaaaggctggaactgtgaggtgtaggtgtgagaacacagcaatgcttggcgagagaaagaattgcaatctgccaggaattgttgtggaccttcctacactgactgagaaggataaagaagacattttgggatggggtgtgccaaatgacatagacatgattgctctgtcgtttgtccgtaaaggatcagatttggttaccgtcagacaacttcttggacagcatgcaaagcgcatcaagctgatgtcaaaggtttgtaatcattatcactattttttgggtcctgttaggttttcagttccacagtttgcttaagtatttaattaccaggacggttgagtgacaatacttctcagttagatgctttaattaatgttttgttgtccctttctggtatatactggagccagaattgtttggtctctgcctttgtacattgaccatttgacccctttttgtgctatattaggtaataaaatgcaattttgttccttttctatgtgcttgaacaactagtttcagaataaccttttttcacgtttgcttgttcaaatagcagctacttattttctcatgcactttcactgatagggtttagcttgttgcctgatatgcattaaaatgaggaattcaggttattgcatttctttgctgctaactgaaattttggatctagatggttcaccgtgttataaatgacctgtcttactgccatgatgataaaatatattcgtgatgatgataatacatatgaggtgcctggtaatttaccatgctggttatgttgctgtgcgtctaatttctttcttaactttttccttgtttttaggttgaaaaccaagagggtgttgtaaacttcgatgagatcttgagggaaacggatgcatttatggttgctagaggtgatcttggaatggagattccagttgagaagatattccttgcacagaagatgatgatttacaagtgcaaccttgctggaaagcctgttgtgactgctactcagatgcttgagtcgatgatcaaatcaccacgtccaactcgtgctgaggcaactgacgttgcaaatgcagttcttgatggaactgactgcgtcatgcttagtggagagagtgctgctggagcataccctgaagtagctgtgaagatcatggcacgtatatgtgttgaggcagagtcttcccttgacaacgaagctgtcttcaaggagatgatcaggtctgcgccccttccgatgagcccattggagtctctcgcatcctctgctgtacgcacagccaacaaggccaaggcagccctgattgttgtcttgactcgtggtggtaccacggcaaagctggttgccaagtatcgtcccagggttccaatcctctctgtggttgtccccgtgttgacaaccgattcattcgactggacaatcagctcggagggcccagcaaggcacagcctaatctacagaggtcttgttcctctcctggctgagggttctgccaaagccaccgattcggagtcgacagaggtcatccttgatgctgctctcaagtcagctgtacagaagcagttgtgcaagcctggtgatgctgttgttgctctgcaccgtattggcgtcgcatccgtgatcaagatctgcatcgtgaagtaatcgctacacggtgtcttcttagcgccattttgctgggttaagtggcaaaacaaaaactgctatgcttgtttttactcccatagtccatctttgggagacaatttaattaccgtatcagcccctgcagttcgcagggtgcctttcgttacatagatttgctggtaaaatgaggagagatagaactccattatgtagagctctatttatcgtcgatgccatgtagtatggcaggtggtttctattccatgcctgcacaattttctggtagctactgtgttgtttgattactgttttttcaggtgatgtcgatggagttgataataatattggca</dnaseqindica> |
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