Difference between revisions of "Os08g0424500"
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==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
| + | [[File:function.png|right|thumb|500px|'''Figure1.''' ''2AP Levels and Their Significant Differences among Nontransgenic Lines and Three Kinds of Transgenic Lines Carrying Different Badh2 CDS Driven by the CaMV35S Promoter.From(from reference <ref name="ref1"/>).'']] | ||
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This gene is reported as BADH2, encoding betaine aldehyde dehydrogenase (BADH2) inhibits the synthesis of 2-acetyl-1-pyrroline (2AP), a potent flavor component in rice fragrance.In rice the nitrogen in the pyrroline ring of proline becomes the nitrogen in the pyrroline ring of 2AP while the carboxyl group of proline is removed and replaced with an acetyl group from another source found that in L. hilgardii the acetyl group of 2AP can be derived from fructose when either ethanol or acetaldehyde are supplied in excess. They further suggested D1pyrroline, a product of proline catabolism via putrescine oxidation, is the immediate precursor of the pyrroline ring of 2AP and the acetyl group was most likely formed from reaction with acetyl-CoA or acetaldehyde in either a chemical or enzymatic reaction. Additionally, detailed precursor studies have revealed that the formation of 2AP in Bacillus cerus proceeds via acetylation of D1pyrroline. | This gene is reported as BADH2, encoding betaine aldehyde dehydrogenase (BADH2) inhibits the synthesis of 2-acetyl-1-pyrroline (2AP), a potent flavor component in rice fragrance.In rice the nitrogen in the pyrroline ring of proline becomes the nitrogen in the pyrroline ring of 2AP while the carboxyl group of proline is removed and replaced with an acetyl group from another source found that in L. hilgardii the acetyl group of 2AP can be derived from fructose when either ethanol or acetaldehyde are supplied in excess. They further suggested D1pyrroline, a product of proline catabolism via putrescine oxidation, is the immediate precursor of the pyrroline ring of 2AP and the acetyl group was most likely formed from reaction with acetyl-CoA or acetaldehyde in either a chemical or enzymatic reaction. Additionally, detailed precursor studies have revealed that the formation of 2AP in Bacillus cerus proceeds via acetylation of D1pyrroline. | ||
The intact BADH2 protein encoded by the complete Badh2 gene sequence was shown to influence this critical switch, possibly due to its strong AB-ald dehydrogenase activity. From its activity in vitro, we conclude that, in nonfragrant rice, the BADH2 enzyme converts AB-ald into GABA, inhibiting 2AP biosynthesis. In fragrant rice lacking intact BADH2, failure to convert AB-ald into GABA due to the absence of BADH2 enzymatic activity results in AB-ald accumulation, which activates 2AP biosynthesis. Interestingly, the low level of BADH2 detected in some transgenic lines overexpressing Badh2 might not completely inhibit the consumption of AB-ald, resulting in a small quantity of 2AP. | The intact BADH2 protein encoded by the complete Badh2 gene sequence was shown to influence this critical switch, possibly due to its strong AB-ald dehydrogenase activity. From its activity in vitro, we conclude that, in nonfragrant rice, the BADH2 enzyme converts AB-ald into GABA, inhibiting 2AP biosynthesis. In fragrant rice lacking intact BADH2, failure to convert AB-ald into GABA due to the absence of BADH2 enzymatic activity results in AB-ald accumulation, which activates 2AP biosynthesis. Interestingly, the low level of BADH2 detected in some transgenic lines overexpressing Badh2 might not completely inhibit the consumption of AB-ald, resulting in a small quantity of 2AP. | ||
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===Expression=== | ===Expression=== | ||
Revision as of 05:39, 1 June 2014
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Contents
Annotated Information
Function
This gene is reported as BADH2, encoding betaine aldehyde dehydrogenase (BADH2) inhibits the synthesis of 2-acetyl-1-pyrroline (2AP), a potent flavor component in rice fragrance.In rice the nitrogen in the pyrroline ring of proline becomes the nitrogen in the pyrroline ring of 2AP while the carboxyl group of proline is removed and replaced with an acetyl group from another source found that in L. hilgardii the acetyl group of 2AP can be derived from fructose when either ethanol or acetaldehyde are supplied in excess. They further suggested D1pyrroline, a product of proline catabolism via putrescine oxidation, is the immediate precursor of the pyrroline ring of 2AP and the acetyl group was most likely formed from reaction with acetyl-CoA or acetaldehyde in either a chemical or enzymatic reaction. Additionally, detailed precursor studies have revealed that the formation of 2AP in Bacillus cerus proceeds via acetylation of D1pyrroline. The intact BADH2 protein encoded by the complete Badh2 gene sequence was shown to influence this critical switch, possibly due to its strong AB-ald dehydrogenase activity. From its activity in vitro, we conclude that, in nonfragrant rice, the BADH2 enzyme converts AB-ald into GABA, inhibiting 2AP biosynthesis. In fragrant rice lacking intact BADH2, failure to convert AB-ald into GABA due to the absence of BADH2 enzymatic activity results in AB-ald accumulation, which activates 2AP biosynthesis. Interestingly, the low level of BADH2 detected in some transgenic lines overexpressing Badh2 might not completely inhibit the consumption of AB-ald, resulting in a small quantity of 2AP.
Expression
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Evolution
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Labs working on this gene
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References
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Structured Information
| Gene Name |
Os08g0424500 |
|---|---|
| Description |
Similar to Betaine aldehyde dehydrogenase |
| Version |
NM_001068368.1 GI:115476473 GeneID:4345606 |
| Length |
6153 bp |
| Definition |
Oryza sativa Japonica Group Os08g0424500, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 8:20467829..20473981 |
| Sequence Coding Region |
20467975..20468083,20468183..20468325,20469378..20469458,20469554..20469702,20470400..20470492 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008401:20467829..20473981 source=RiceChromosome08 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008401:20467829..20473981 source=RiceChromosome08 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggccacggcgatcccgcagcggcagctcttcgtcgccggcgagtggcgcgcccccgcgctcggccgccgcctccccgtcgtcaaccccgccaccgagtcccccatcggcgagatcccggcgggcacggcggaggacgtggacgcggcggtggcggcggcgcgggaggcgctgaagaggaaccggggccgcgactgggcgcgcgcgccgggcgccgtccgggccaagtacctccgcgcaatcgcggccaagataatcgagaggaaatctgagctggctagactagagacgcttgattgtgggaagcctcttgatgaagcagcatgggacatggacgatgttgctggatgctttgagtactttgcagatcttgcagaatccttggacaaaaggcaaaatgcacctgtctctcttccaatggaaaactttaaatgctatcttcggaaagagcctatcggtgtagttgggttgatcacaccttggaactatcctctcctgatggcaacatggaaggtagctcctgccctggctgctggctgtacagctgtactaaaaccatctgaattggcttccgtgacttgtttggagcttgctgatgtgtgtaaagaggttggtcttccttcaggtgtgctaaacatagtgactggattaggttctgaagccggtgctcctttgtcatcacaccctggtgtagacaaggttgcatttactgggagttatgaaactggtaaaaagattatggcttcagctgctcctatggttaagcctgtttcactggaacttggtggaaaaagtcctatagtggtgtttgatgatgttgatgttgaaaaagctgttgagtggactctctttggttgcttttggaccaatggccagatttgcagtgcaacatcgcgtcttattcttcataaaaaaatcgctaaagaatttcaagaaaggatggttgcatgggccaaaaatattaaggtgtcagatccacttgaagagggttgcaggcttgggcccgttgttagtgaaggacagtatgagaagattaagcaatttgtatctaccgccaaaagccaaggtgctaccattctgactggtggggttagacccaagcatctggagaaaggtttctatattgaacccacaatcattactgatgtcgatacatcaatgcaaatttggagggaagaagtttttggtccagtgctctgtgtgaaagaatttagcactgaagaagaagccattgaattggccaacgatactcattatggtctggctggtgctgtgctttccggtgaccgcgagcgatgccagagattaactgaggagatcgatgccggaattatctgggtgaactgctcgcaaccctgcttctgccaagctccatggggcgggaacaagcgcagcggctttggacgcgagctcggagaagggggcattgacaactacctaagcgtcaagcaagtgacggagtacgcctccgatgagccgtggggatggtacaaatccccttccaagctgtaa</cdnaseq> |
| Protein Sequence |
<aaseq>MATAIPQRQLFVAGEWRAPALGRRLPVVNPATESPIGEIPAGTA EDVDAAVAAAREALKRNRGRDWARAPGAVRAKYLRAIAAKIIERKSELARLETLDCGK PLDEAAWDMDDVAGCFEYFADLAESLDKRQNAPVSLPMENFKCYLRKEPIGVVGLITP WNYPLLMATWKVAPALAAGCTAVLKPSELASVTCLELADVCKEVGLPSGVLNIVTGLG SEAGAPLSSHPGVDKVAFTGSYETGKKIMASAAPMVKPVSLELGGKSPIVVFDDVDVE KAVEWTLFGCFWTNGQICSATSRLILHKKIAKEFQERMVAWAKNIKVSDPLEEGCRLG PVVSEGQYEKIKQFVSTAKSQGATILTGGVRPKHLEKGFYIEPTIITDVDTSMQIWRE EVFGPVLCVKEFSTEEEAIELANDTHYGLAGAVLSGDRERCQRLTEEIDAGIIWVNCS QPCFCQAPWGGNKRSGFGRELGEGGIDNYLSVKQVTEYASDEPWGWYKSPSKL</aaseq> |
| Gene Sequence |
<dnaseqindica>147..255#355..497#1550..1630#1726..1874#2572..2664#2796..2919#3004..3069#3260..3326#4019..4095#4451..4564#4965..5042#5124..5275#5379..5439#5711..5817#5916..6006#agaacagagcactccctctcccaccaccactccacacctgacaccacgcgggctgcctccatcgtcatcgatccatctccgtatctctcaccgaccccaaatcgcacagctccagctcctcctcgatccccgtcgcgatcggcgagatggccacggcgatcccgcagcggcagctcttcgtcgccggcgagtggcgcgcccccgcgctcggccgccgcctccccgtcgtcaaccccgccaccgagtcccccatcggtaccctcctcttcaccctctccaccctctgcttctgcctctgattagcctttttgttgttgttgttgttgctgctgttttttgcgtgtcggtgcgcaggcgagatcccggcgggcacggcggaggacgtggacgcggcggtggcggcggcgcgggaggcgctgaagaggaaccggggccgcgactgggcgcgcgcgccgggcgccgtccgggccaagtacctccgcgcaatcgcggccaaggtagggtggtgactacccccacccccccccccccccccaacgcgacccgcgtgcgtgtgttccgtacagggggaggagctccgcgtggctctccagtaggtttttgagccccaaatcgatcgatatgctctagttttaagtttgctgcttaaattcctcaagggtttagtttgcaaccaaatccttattttagcttcggtataagccccccatatgatgtgcgtgcgtcggcatcggaagtgcgtatcctctgttctggactaggaattggccataggttgatcgacagttcgagtattctgcttctgtttggaataagttggaagcatggctgattgtgtatctggatgctgtttttgtggtgattcgtttcaagctcttgttaattgatgggttcaagcggagagggtgcgcaacaacaagtgtatatggctcacggccatgggtgtgcacatttgattggtgcgcaacaacaagtgtatattgtttgtgtgcttcgttagttggcaggtcctagtcactaaatcactattggattggtactagttacttttgtgccttgacgatgggactggattactagccttttggttgcctttgtggtattccgttgttatgggcctgttgatggatggatccctttaatttctagtgccaaatgcatgctagatttctcacagtttttctcttcaggttatatttctcgtatttccttttcctaaaggattgctttttcatgtattttctggcatatataggttattattattattattctccagaacaagattacccatattatggatcactagtgtacacttttttggatgaaaaacctacttactgaaagtaaaacagtgaccagtgcacactttacttgaactgtcaaaccatcaattttctagcaaagcaggggatgctagccttccagtctaaatgacagtaaactactatacttttgtccgtaggtttggaaatatgctaatttctatcataaaaattttcatggcatatgcgagcattttatgatcaccttttccctttttcttcagataatcgagaggaaatctgagctggctagactagagacgcttgattgtgggaagcctcttgatgaagcagcatgggacatggtatgtggccagttatccactgtatgaatatgtagttgcctacacagcaatctttcctgaacatgaatcctgatgtatgatattccatttgtcaggacgatgttgctggatgctttgagtactttgcagatcttgcagaatccttggacaaaaggcaaaatgcacctgtctctcttccaatggaaaactttaaatgctatcttcggaaagagcctatcggtgtagttgggttgatcacaccttggtatttcacatttttctctcatcctgcgcttatatttatttatgacccaagcatggtactaaatagtactagtaacatgcatatactgaatgagtttacaactttacatgatttttttgaactatgaaagttgaagacatttgagattttattcctcttctcttgtgcaaacatattattgtctcacaaattgtacctagcagctactctctccgtttcatattataagtcgtttgacttttttcctagtcaaaatgtgttaagtttgaccaagtttatagaaaaatttagcaacatctaaaatatcaaagtcatgttttagtgttttttcaggctctcatgtaagcaattttgatgtgccctctcctttcttcttaatataatgatacacagctcttgtgtattcaaaggaaaatatatatatatataatgatacacacctctcctccgtgttaatgcagctcatttgttctgtcccggttcaaatatctatttttctcatatgttgtcagcatgattcacttaatttagtatatagaagatgccattatttatgtctggaatcttactgcagaagggaaaacaattgataacggaattgattgcattctaatttgttgtttctttgttatgttcttatcgacaattacaaatttgattctgagaatcatgttcgggatgtgtatttctactgcaggaactatcctctcctgatggcaacatggaaggtagctcctgccctggctgctggctgtacagctgtactaaaaccatctgaattggcttccgtgtaagtttaacatgttaacttgttaatgtcatacccatgctagttgcaatgacatttgattttaaaatgttgtggcatgtccatgctgcaagcaatgtaatttgaaatctctctctatcattaattaccaggacttgtttggagcttgctgatgtgtgtaaagaggttggtcttccttcaggtgtgctaaacatagtgactggattaggttctgaagccggtgctcctttgtcatcacaccctggtgtagacaaggtacagctattcctcctgtaatcatgtataccccatcaatggaaatgatattcctctcaatacatggtttatgttttctgttaggttgcatttactgggagttatgaaactggtaaaaagattatggcttcagctgctcctatggttaaggtttgtttccaaatttctgtggatattttttgttctctttctactaactctctattatcaattctcaatgttgtccttttcttttaactcctttactttttagaattgtgatcaagacactttgagcatcattctagtagccagttctatcctgtttcttacctttttatggttcgtcttttcttgacagcctgtttcactggaacttggtggaaaaagtcctatagtggtgtttgatgatgttgatgttgaaaaaggtacatgccacttgctatgattaactaattctgaagtgcgggactttgtaaagcacttaactgagctggatgctagacccccaaaagccctttttggtgtcttgggcttgttgcagaaatactggtcccagacgagcaggatgcaagaaaattaactacttttgccactgattagtatttcttagaagttacacctcaaggattagcaatactttcttaaaatgtgctattgattaaaaagatgtcctgtattattttgagcagatcttgtactggttgatcggcttgcatgaaaatattgttgaggattataatgccatgccaactgagtaaagaaaagagttgtaaaatatgttatgcaacatgaatatatatgtgatttcatttttcctttttcttttcgtggcaaggaaggcagttaggaaggactgatgtgaaaagcacaagtactattcttagttctggaaaactgtgttctttattttcctaactacaattcaccttgattagtcagtaacttgatattggcaattctagctgattatgaattctgtttatatttcactaattttgaatctttaattacattttatggttgaaatttaacgttttgtctggttatggactctgtttgtattcactcaatttggatcttccattagatttcattgttggtccttcttcttgtacagctgttgagtggactctctttggttgcttttggaccaatggccagatttgcagtgcaacatcgcgtcttattcttcatgtaagcattgaatatatccgtcaatcataatctattgttgtacttgattttttttctgatcaactcctgagttcagattattatatgatgccattactattgcacagagcgaataaaattgtatttatgcacagcatgtattttgagtaatatatgcattgcctattatttaatatatagattgtagcacttaattttgtgtccatgtctctatgatgtttattactttattattgccggcatgaagcaactttgaactctatgttgatcttgaactaaaattgaaattaattggcttattgctattaatgatatagctttcagcttcttgctcctgaccatgaaagttttgcagaaaaaaatcgctaaagaatttcaagaaaggatggttgcatgggccaaaaatattaaggtgtcagatccacttgaagagggttgcaggcttgggcccgttgttagtgaaggacaggtaccacatgtaaactttttctaaattcaaaaaagaaatgccactgatcaatggtaggtccttccaagccttattgctggattgttgcactgttttgtcaattttgtgtaatatagttctgaatgaattagtcggtgtatgctcttgctagttgctagtatgtggtacagggtcttcctactttgagcaaattcgtgttaaaatgcattgatgaaaaggccacctttccgtaggtttatcttgtcataatttaaaccccaataaaattttaattttttgttttgaccccatggcactttaatgaaatcacttagccatgagcttttgtatatattttcaaagcaccagaatgtttagatggtttgttggaaatcttacacatcctattgccttgtgtcagtatgagaagattaagcaatttgtatctaccgccaaaagccaaggtgctaccattctgactggtggggttagacccaaggtaataatctactacacggttgtatatataggtacccacatatcattatgaagtagaaataatcttgtatgtttttgtcagcatctggagaaaggtttctatattgaacccacaatcattactgatgtcgatacatcaatgcaaatttggagggaagaagtttttggtccagtgctctgtgtgaaagaatttagcactgaagaagaagccattgaattggccaacgatactcagtgagttttttttttaatacagttcattgtcctgttcaatcttgcagcatatgtatatactctgtggcatatgaacttattctgctactactacttttgatagttatggtctggctggtgctgtgctttccggtgaccgcgagcgatgccagagattaactgaggtatatccaagtgaagggggttggcattgtttgattcatatgacatggttgcatcaagctgatattcaagaatctcatttattacttgcattctatgcatctccagttcttccctggactccggtcaatgttaatatagtttgtttgctagtagtatgctactccaattaagttgctcttcacttccacatcatctgatccatgactttatatttgaccccttttttttgcaaaagaaagggaaatacttaacgaaaatttcctactgcaggagatcgatgccggaattatctgggtgaactgctcgcaaccctgcttctgccaagctccatggggcgggaacaagcgcagcggctttggacgcgagctcggagaagggtgggtagcacacaacaatctcactttaaaacaccatttcgatcgtctgatgatctcgacctgacatcatgcctttggtattttcattcacttttcaggggcattgacaactacctaagcgtcaagcaagtgacggagtacgcctccgatgagccgtggggatggtacaaatccccttccaagctgtaatgtaatatgcgcatacgcatgacacgcccatctgttctgatcgtttggaataagaggccatagtatgacgggatggactttgtgcatatcgatgtttgatttgccatgtgctgagcctggaaataaaaaaggttatagttggagact</dnaseqindica> 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| External Link(s) |
- ↑ Cite error: Invalid
<ref>tag; no text was provided for refs namedref1
