Difference between revisions of "Os09g02650"
(→Structure) |
(→Structure) |
||
| Line 20: | Line 20: | ||
''BC12'' harbors a conserved motor domain at the N-terminus, a neck linker after the motor domain,a long coiled-coil region and the NLS at the C-terminus. The coiled-coil region is responsible for dimerization. BC12 shows microtubule-dependent ATPase activity, and the neck linker plays a critical role for this activity. | ''BC12'' harbors a conserved motor domain at the N-terminus, a neck linker after the motor domain,a long coiled-coil region and the NLS at the C-terminus. The coiled-coil region is responsible for dimerization. BC12 shows microtubule-dependent ATPase activity, and the neck linker plays a critical role for this activity. | ||
[[File:structure 1.png]] | [[File:structure 1.png]] | ||
| + | |||
| + | == Sequence == | ||
| + | |||
| + | The sequence of this gene are as following: | ||
| + | 1 ATGACGATGG AGCACGGCGA GGATTGCTGC GTCAAGGTGG CCGTCCATGT | ||
| + | 51 CCGCCCGCTC ATCGGCGACG AGAAGCTTCA GGGCTGTAAG GACTGCGTCT | ||
| + | 101 CCGTCGTCTC CGGCAAGCCG CAGGTTCAGA TCGGGAGCCA TTCCTTCACC | ||
| + | 151 TTCGACCATG TGTACGGCAG CTCCGGCACG CCGTCGGCGG CCATGTTCGA | ||
| + | 201 GGAGTGCGTG GCGCCGCTCG TCGACGGCCT CTTCCAGGGC TACAACGCCA | ||
| + | 251 CCGTGCTCGC ATACGGCCAG ACTGGGTCAG GGAAGACCTA CACCATGGGG | ||
| + | 301 ACGGCCTGCA AGGAGGGCTC ACACATTGGG ATTATCCCGC GTGCCATGGC | ||
| + | 351 CACGCTGTTC GACAAGATCG ACAAGCTCAA GAACCAAGTA GAGTTCCAGC | ||
| + | 401 TGCGCGTTTC GTTTATTGAG ATTCTGAAAG AAGAGGTGAG GGATTTGCTT | ||
| + | 451 GATCCTGCTA CTGCTGCTGT TGGCAAACTT GAGAATGGAA ATGGACATGC | ||
| + | 501 AACCAAGTTG TCGGTTCCAG GTAAACCCCC TGTTCAGATC CGGGAGGCGT | ||
| + | 551 CGAACGGGGT CATAACGTTA GCAGGATCGA CCGAAGTGCA TGTCACTACC | ||
| + | 601 CAGAAAGAAA TGACGGCATG CCTTGAGCAA GGATCTCTGA GTCGTGCAAC | ||
| + | 651 TGGAAGTACC AACATGAACA ACCAATCAAG TCGTTCCCAT GCCATCTTCA | ||
| + | 701 CAATCACATT GGAGCAGATG CGCAAAGCAG ATCCCATCAT GACTTTAGAT | ||
| + | 751 GGAATGCCTA TTGAAGAGAT GAATGAAGAC TATCTCTGTG CCAAACTCCA | ||
| + | 801 CTTAGTAGAT CTTGCTGGAT CAGAACGTGC TAAAAGAACT GGTTCTGATG | ||
| + | 851 GCCTTCGGTT TAAGGAAGGT GTTCATATCA ACAGAGGACT TCTTGCTCTT | ||
| + | 901 GGCAATGTCA TCAGTGCTCT CGGTGATGAG AAAAAGAGGA AAGAAGGCGC | ||
| + | 951 TCATGTTCCT TACCGGGACA GCAAACTCAC TCGCCTTCTG CAGGACTCTC | ||
| + | 1001 TAGGTGGAAA CAGCAAGACT GTAATGATAG CCTGTATTAG TCCAGCAGAT | ||
| + | 1051 ATCAATGCTG AAGAAACACT GAACACTTTG AAATATGCTA ACCGCGCTCG | ||
| + | 1101 TAACATCCAG AATAAGCCAA TTGTAAATAG AAATCCTGTT GCTGATGAGA | ||
| + | 1151 TGAAAAGGAT GCGCCAGCAA ATTGAATACT TGCAAGCAGA ACTCGTTTCA | ||
| + | 1201 GCTCGAGGAG GAGTTGTCTT AGATGATGTT CAGGGTCTCA GGGAAAGGAT | ||
| + | 1251 CTCAATGCTT GAACAGAAAA ATGAAGACCT TTGCAGGGAA CTGTATGACC | ||
| + | 1301 TTCGCAACCA TGGTTACACT GATCCTTGTG AACCTGAACT GCAAAAAATT | ||
| + | 1351 GGAACTGGTT ACACTAAAGG TGAAGGGCTC AAAAGAAGCT TGCAAAGTAC | ||
| + | 1401 AGAACCATTT GATGTCCCCA TGACTGACTC AGTAAGAGCA GGCAGCCCTA | ||
| + | 1451 AAGATATTGA TGATGAAGTG GCCAAAGAAT GGGAACACAC AATGCTGCAG | ||
| + | 1501 GATAGCATGG GCAAAGAGTT GAATGAATTA AACAGACAAC TGGAGCAAAA | ||
| + | 1551 GGAGTCTGAG ATGAAAATGT ATGGATCTGA CACTGTTGCA CTTAAACAAC | ||
| + | 1601 ACTTTGGAAA GAAACTTTTG GAGCTTGAAG AAGAGAAAAG AGCTGTACAG | ||
| + | 1651 CAAGAAAGGG ACAGATTGTT AGCTGAAGTT GAAAGTCTAA ATGCTGATGG | ||
| + | 1701 ACAAACACAC AAGTTGCGAG ATGCCCAGCT GCAAAAATTA AAAACCCTTG | ||
| + | 1751 AAGCACAGAT TCTAGACCTC AAGAAAAAGC AGGAGAACCA AGTTCAACTT | ||
| + | 1801 CTGAAGGAAA AGCAAAAGAG TGATGAAGCT GCTAAGAAGT TGCAGGAAGA | ||
| + | 1851 AATTCATTCT ATAAAGGCAC AGAAGGTTCA ACTACAACAT AAAATCAAAC | ||
| + | 1901 AAGAAGCGGA ACAATTCCGG CAATGGAAGG CTACCCGTGA AAAGGAACTC | ||
| + | 1951 CTGCAGTTGA GGAAAGAGGG GCGGCGAAAT GAGTATGAAC GCCACAAACT | ||
| + | 2001 TCAAGCACTT AATCAGCGGC AGAAGTTGGT TTTGCAGAGG AAGACTGAAG | ||
| + | 2051 AAGCTGCGAT GGCTACCAAA AGGCTGAAAG AGTTACTAGA GGCTCGGAAA | ||
| + | 2101 TCATCAGGAC GTGACAACTC AGGCATGAAT GGTACTTCTC CTGGCTCTCA | ||
| + | 2151 TATGACTGAG AAATCATTGC AAAAATGGCT AGAGCAAGAT TTGGAAGTCA | ||
| + | 2201 TGGTGCATGT TCATGAAGTT CGAAACGAAT ATGAAAAGCA AAGTCAATTG | ||
| + | 2251 CGTGCTGCAC TTGGTGAGGA GCTTGCCATT TTAAAACAAG AAGATGTCAT | ||
| + | 2301 GTCTGGTGCA GCTAGTCCGC CCAGAGGGAA GAATGGAAAC TCTAGGGCAA | ||
| + | 2351 ATACTTTGTC ACCAAATGCA AGACAAGCTA GGATAGCATC ACTTGAAAGC | ||
| + | 2401 ATGGTGACAA TATCTTCAAA TACTCTCGTT GCTATGGCTT CTCAACTCTC | ||
| + | 2451 AGAAGCTGAA GAAAGAGAGC GTGCATTCTC TGGGCGTGGT CGGTGGAATC | ||
| + | 2501 AGTTGCGTTC AATGGCAGAG GCAAAGAGTT TACTGCAGTA TATATTTAAC | ||
| + | 2551 GTTGCTGCAG ATGCAAGATG CCAAGTAAGG GAGAAAGAGA TGGAGATCAA | ||
| + | 2601 GGAAATGAAG GAGCAAATGA CAGAGCTTGT GACCATCCTT CGACACAGCG | ||
| + | 2651 AATCACGTAG AAGGGAAACG GAAAAGCAGC TTAAGCAAAG AGAGCAGGCA | ||
| + | 2701 GCTGTAACTG CCACTACATC TCCAGGAAAC GGAAATGGTT CAGTGAAGCA | ||
| + | 2751 CTCTGCTGAT GACTCCAACA CACCATTGTC ACCAGTTGCG GTGCCTGCAC | ||
| + | 2801 AGAAGCAGCT GAAGTACTCT GCTGGAATTG TAAATAGCCC CAGCAAAGGG | ||
| + | 2851 GTTCCTGCAT TCAACAAACA ACATCTTAAG ATGGTTCCTA TGGCACAGTT | ||
| + | 2901 GCCTGTTGGC AAGAAGGTTT CAATAGCAGG GCAATCAGGA AAACTTTGGA | ||
| + | 2951 GATGGAAAAG AAGCCACCAC CAGTGGCTAC TGCAGTTCAA GTGGAAGTGG | ||
| + | 3001 CAAAAGCCCT GGAAATTGTC CGAGATGATT CGACACAGTG ACGAAACGAT | ||
| + | 3051 GACAAGGACT CGACCCAGAC CCCAGCTTCT TCCTCATAGA CCTCAAAGAG | ||
| + | 3101 TGATGTGA | ||
== Knowledge extension == | == Knowledge extension == | ||
''FRA1''(FRAGILE FIBER 1) is the homolog gene of ''BC12'' in Arabidopsis. FRA1 which is found in the cytoplasm,was the first functionally identified kinesin-4 member in plants. The ''fra1'' mutant exhibits reduced plant height and fragile fibers as a result of aberrant deposition of cellulose microfibrils in the cell wall. | ''FRA1''(FRAGILE FIBER 1) is the homolog gene of ''BC12'' in Arabidopsis. FRA1 which is found in the cytoplasm,was the first functionally identified kinesin-4 member in plants. The ''fra1'' mutant exhibits reduced plant height and fragile fibers as a result of aberrant deposition of cellulose microfibrils in the cell wall. | ||
Revision as of 09:46, 1 June 2014
The rice Brittle Culm 12 (BC12) gene encodes a dual-targeting kinesin-4 protein, controls cell-cycle progression and wall properties in rice.
Function
This gene encodes a kinesin-4 protein, which is participated in cell-cycle progression, cellulose microfibril deposition and cell wall composition in the monocot plant rice. bc12 mutants display dwarfism resulting from a significant reduction in cell number and brittleness due to an alteration in cellulose microfibril orientation and wall composition. Similar to the typical kinesin-4 in animals,BC12 has an nuclear locational signal (NLS) and functions in mitosis. BC12 is present in both the nucleus and cytoplasm and associated with microtubule arrays during cell division. BC12 interacts with CDKA;3 (cyclin-dependent kinase) and probably undergoing phosphorylation for the purpose of regulation. BC12 is essential to exert multiple roles in cell division and cell wall biogenesis in rice.
Mutation
bc12: A 26bp deletion in the 4th exon of one open reading frame (ORF), results in a frameshift. which is related with the dwarfism and brittleness phenotype.
Expression pattern
BC12 is universally expressed in all organs. with higher expression in panicles and culms. RNA in situ hybridization reveals that BC12 is expressed in young tissues, includeing the initiating adventitious roots, primary root tips, flower primordia and intercalary meristems, all tissues in which cells are undergoing vigorous cell division.
Structure
The CDS coordinates(5'-3') of this gene is from 1158082 to 1167873. The ORF of the BC12 is 3108 nucleotides long and encodes a kinesin protein of the kinesin-4 subfamily which is 1036 amino acid residues long. The predicted molecular weight is about 116386, and the predicted pI is 9.4858.
BC12 harbors a conserved motor domain at the N-terminus, a neck linker after the motor domain,a long coiled-coil region and the NLS at the C-terminus. The coiled-coil region is responsible for dimerization. BC12 shows microtubule-dependent ATPase activity, and the neck linker plays a critical role for this activity.
Sequence
The sequence of this gene are as following:
1 ATGACGATGG AGCACGGCGA GGATTGCTGC GTCAAGGTGG CCGTCCATGT 51 CCGCCCGCTC ATCGGCGACG AGAAGCTTCA GGGCTGTAAG GACTGCGTCT 101 CCGTCGTCTC CGGCAAGCCG CAGGTTCAGA TCGGGAGCCA TTCCTTCACC 151 TTCGACCATG TGTACGGCAG CTCCGGCACG CCGTCGGCGG CCATGTTCGA 201 GGAGTGCGTG GCGCCGCTCG TCGACGGCCT CTTCCAGGGC TACAACGCCA 251 CCGTGCTCGC ATACGGCCAG ACTGGGTCAG GGAAGACCTA CACCATGGGG 301 ACGGCCTGCA AGGAGGGCTC ACACATTGGG ATTATCCCGC GTGCCATGGC 351 CACGCTGTTC GACAAGATCG ACAAGCTCAA GAACCAAGTA GAGTTCCAGC 401 TGCGCGTTTC GTTTATTGAG ATTCTGAAAG AAGAGGTGAG GGATTTGCTT 451 GATCCTGCTA CTGCTGCTGT TGGCAAACTT GAGAATGGAA ATGGACATGC 501 AACCAAGTTG TCGGTTCCAG GTAAACCCCC TGTTCAGATC CGGGAGGCGT 551 CGAACGGGGT CATAACGTTA GCAGGATCGA CCGAAGTGCA TGTCACTACC 601 CAGAAAGAAA TGACGGCATG CCTTGAGCAA GGATCTCTGA GTCGTGCAAC 651 TGGAAGTACC AACATGAACA ACCAATCAAG TCGTTCCCAT GCCATCTTCA 701 CAATCACATT GGAGCAGATG CGCAAAGCAG ATCCCATCAT GACTTTAGAT 751 GGAATGCCTA TTGAAGAGAT GAATGAAGAC TATCTCTGTG CCAAACTCCA 801 CTTAGTAGAT CTTGCTGGAT CAGAACGTGC TAAAAGAACT GGTTCTGATG 851 GCCTTCGGTT TAAGGAAGGT GTTCATATCA ACAGAGGACT TCTTGCTCTT 901 GGCAATGTCA TCAGTGCTCT CGGTGATGAG AAAAAGAGGA AAGAAGGCGC 951 TCATGTTCCT TACCGGGACA GCAAACTCAC TCGCCTTCTG CAGGACTCTC
1001 TAGGTGGAAA CAGCAAGACT GTAATGATAG CCTGTATTAG TCCAGCAGAT 1051 ATCAATGCTG AAGAAACACT GAACACTTTG AAATATGCTA ACCGCGCTCG 1101 TAACATCCAG AATAAGCCAA TTGTAAATAG AAATCCTGTT GCTGATGAGA 1151 TGAAAAGGAT GCGCCAGCAA ATTGAATACT TGCAAGCAGA ACTCGTTTCA 1201 GCTCGAGGAG GAGTTGTCTT AGATGATGTT CAGGGTCTCA GGGAAAGGAT 1251 CTCAATGCTT GAACAGAAAA ATGAAGACCT TTGCAGGGAA CTGTATGACC 1301 TTCGCAACCA TGGTTACACT GATCCTTGTG AACCTGAACT GCAAAAAATT 1351 GGAACTGGTT ACACTAAAGG TGAAGGGCTC AAAAGAAGCT TGCAAAGTAC 1401 AGAACCATTT GATGTCCCCA TGACTGACTC AGTAAGAGCA GGCAGCCCTA 1451 AAGATATTGA TGATGAAGTG GCCAAAGAAT GGGAACACAC AATGCTGCAG 1501 GATAGCATGG GCAAAGAGTT GAATGAATTA AACAGACAAC TGGAGCAAAA 1551 GGAGTCTGAG ATGAAAATGT ATGGATCTGA CACTGTTGCA CTTAAACAAC 1601 ACTTTGGAAA GAAACTTTTG GAGCTTGAAG AAGAGAAAAG AGCTGTACAG 1651 CAAGAAAGGG ACAGATTGTT AGCTGAAGTT GAAAGTCTAA ATGCTGATGG 1701 ACAAACACAC AAGTTGCGAG ATGCCCAGCT GCAAAAATTA AAAACCCTTG 1751 AAGCACAGAT TCTAGACCTC AAGAAAAAGC AGGAGAACCA AGTTCAACTT 1801 CTGAAGGAAA AGCAAAAGAG TGATGAAGCT GCTAAGAAGT TGCAGGAAGA 1851 AATTCATTCT ATAAAGGCAC AGAAGGTTCA ACTACAACAT AAAATCAAAC 1901 AAGAAGCGGA ACAATTCCGG CAATGGAAGG CTACCCGTGA AAAGGAACTC 1951 CTGCAGTTGA GGAAAGAGGG GCGGCGAAAT GAGTATGAAC GCCACAAACT 2001 TCAAGCACTT AATCAGCGGC AGAAGTTGGT TTTGCAGAGG AAGACTGAAG 2051 AAGCTGCGAT GGCTACCAAA AGGCTGAAAG AGTTACTAGA GGCTCGGAAA 2101 TCATCAGGAC GTGACAACTC AGGCATGAAT GGTACTTCTC CTGGCTCTCA 2151 TATGACTGAG AAATCATTGC AAAAATGGCT AGAGCAAGAT TTGGAAGTCA 2201 TGGTGCATGT TCATGAAGTT CGAAACGAAT ATGAAAAGCA AAGTCAATTG 2251 CGTGCTGCAC TTGGTGAGGA GCTTGCCATT TTAAAACAAG AAGATGTCAT 2301 GTCTGGTGCA GCTAGTCCGC CCAGAGGGAA GAATGGAAAC TCTAGGGCAA 2351 ATACTTTGTC ACCAAATGCA AGACAAGCTA GGATAGCATC ACTTGAAAGC 2401 ATGGTGACAA TATCTTCAAA TACTCTCGTT GCTATGGCTT CTCAACTCTC 2451 AGAAGCTGAA GAAAGAGAGC GTGCATTCTC TGGGCGTGGT CGGTGGAATC 2501 AGTTGCGTTC AATGGCAGAG GCAAAGAGTT TACTGCAGTA TATATTTAAC 2551 GTTGCTGCAG ATGCAAGATG CCAAGTAAGG GAGAAAGAGA TGGAGATCAA 2601 GGAAATGAAG GAGCAAATGA CAGAGCTTGT GACCATCCTT CGACACAGCG 2651 AATCACGTAG AAGGGAAACG GAAAAGCAGC TTAAGCAAAG AGAGCAGGCA 2701 GCTGTAACTG CCACTACATC TCCAGGAAAC GGAAATGGTT CAGTGAAGCA 2751 CTCTGCTGAT GACTCCAACA CACCATTGTC ACCAGTTGCG GTGCCTGCAC 2801 AGAAGCAGCT GAAGTACTCT GCTGGAATTG TAAATAGCCC CAGCAAAGGG 2851 GTTCCTGCAT TCAACAAACA ACATCTTAAG ATGGTTCCTA TGGCACAGTT 2901 GCCTGTTGGC AAGAAGGTTT CAATAGCAGG GCAATCAGGA AAACTTTGGA 2951 GATGGAAAAG AAGCCACCAC CAGTGGCTAC TGCAGTTCAA GTGGAAGTGG 3001 CAAAAGCCCT GGAAATTGTC CGAGATGATT CGACACAGTG ACGAAACGAT 3051 GACAAGGACT CGACCCAGAC CCCAGCTTCT TCCTCATAGA CCTCAAAGAG 3101 TGATGTGA
Knowledge extension
FRA1(FRAGILE FIBER 1) is the homolog gene of BC12 in Arabidopsis. FRA1 which is found in the cytoplasm,was the first functionally identified kinesin-4 member in plants. The fra1 mutant exhibits reduced plant height and fragile fibers as a result of aberrant deposition of cellulose microfibrils in the cell wall.
