BGIOSGA033504

csld4:biosynthesis of cell walls and plant growth

Annotated Information

Fig.1.Vegetative phenotypes of wild-type and mutation

Fig.2. Development of the third-leaf blade in wild-type and mutation.

Fig.3. Reproductive phenotypes of wild-type and mutation .

Fig.4.In situhybridization of OsCSLD4in longitudinal sections of 10-d-old shoot apex

Fig.5.Double-target in situhybridization of OsCSLD4and CDKB2;1in the shoot apex of wild-type plants.

Function

Cellulose synthase-like (CSL) genes are predicted to catalyse the biosynthesis of non-cellulosic polysaccharides such as the β-d-glycan backbone of hemicelluloses and are classified into nine subfamilies (CSLA–CSLH and CSLJ). The CSLD subfamily is conserved in all land plants, and among the nine CSL subfamilies, it shows the highest sequence similarity to the cellulose synthase genes, suggesting that it plays fundamental roles in plant development. The mutants of csld4 showed rolled and narrow leaf blades and a reduction in plant height（figure1）. The narrow leaf blade of sle1 was caused by reduced cell proliferation beginning at the P3 primordial stage （figure 2）. In addition to the size reduction of organs, sle1 mutants exhibited serious developmental defects in pollen formation , anther dehiscence（figure 3）, stomata formation, and cell arrangement in various tissues.

Expression

In situ hybridization experiments showed that OsCSLD4 was expressed in a patchy pattern in developing organs（figure 4）. Double-target in situ hybridization and quantitative RT-PCR analyses revealed that SLE1 was expressed specifically during the M-phase of the cell cycle（figure 5）, and suggested that the cell-cycle regulation was altered in sle1 mutants. These results suggest that the OsCSLD4 protein plays a pivotal role in the M phase to regulate cell proliferation.

Labs working on this gene

1 Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113–8657, Japan 2 National Institute of Genetics, 1111 Yata, Mishima, Shizuoka, 411–8540, Japan

References

1. Takanori Yoshikawa;Mitsugu Eiguchi;Ken-Ichiro Hibara;Jun-Ichi Ito;Yasuo Nagato, Rice SLENDER LEAF 1 gene encodes cellulose synthase-like D4 and is specifically expressed in M-phase cells to regulate cell proliferation. Journal of Experimental Botany, 2013, 64(7): 2049-2061 2. Jiang Hu;Li Zhu;Dali Zeng;Zhenyu Gao;Longbiao Guo;Yunxia Fang;Guangheng Zhang;Guojun Dong;Meixian Yan;Jian Liu;Qian Qian.. Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice. Plant Molecular Biology, 2010, 73(3): 283-292 3. Chao Wu;Yaping Fu;Guocheng Hu;Huamin Si;Shihua Cheng;Wenzhen Liu. Isolation and characterization of a rice mutant with narrow and rolled leaves. Planta, 2010, 232(2): 313-324

Structured Information

Gene Name	BGIOSGA033504
Description	Csld4-cellulose synthase-like family d
Definition	Oryza sativa Indica Group BGIOSGA033504, complete gene.
LocusTag	OSJNBA0056G17.16
Ensembl Transcript ID	BGIOSGA033504-TA
Ensembl Protein ID	BGIOSGA033504-PA
Length	3474
Source	Oryza sativa Indica Group ORGANISM Oryza sativa Indica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 10
Location	Chromosome 10:22128267..22131740
Sequence Coding Region	22128267..22130942,22131033..22131740
Genome Context	<gbrowseImage1> name=IndicaChromosome10:22128267..22131740 source=RiceIndica10 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=IndicaChromosome10:22128267..22131740 source=RiceIndica10 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>ATGGCGTCGAAGGGCATCCTCAAGAACGGCGGTAAGCCGCCGACGGCGCCATCGTCGGCGGCGCCCACGGTGGTGTTCGGTCGGCGGACGGACTCGGGGCGATTCATCAGCTACTCCCGCGACGACCTTGACTCGGAGATCAGCAGCGTGGACTTCCAGGACTACCACGTCCACATCCCCATGACGCCGGACAACCAGCCCATGGACCCTGCCGCCGGCGACGAGCAGCAGTACGTGTCCAGCTCGCTCTTCACCGGCGGGTTCAACAGCGTGACACGCGCGCACGTGATGGAGAAGCAGGCGTCGTCGGCGAGGGCGACGGTGTCGGCGTGCATGGTGCAGGGGTGCGGGTCCAAGATCATGCGCAACGGGCGCGGCGCCGACATCCTCCCCTGCGAGTGCGACTTCAAGATCTGCGTGGACTGCTTCACCGACGCCGTCAAGGGCGGCGGCGGCGTGTGCCCGGGGTGCAAGGAGCCGTACAAGCACGCGGAGTGGGAGGAGGTGGTGTCGGCGTCCAACCACGACGCCATCAACAGGGCGCTGTCGCTGCCGCACGGGCACGGGCATGGCCCCAAGATGGAGAGGCGGCTGTCGCTGGTGAAGCAGAACGGGGGGGCGCCCGGCGAGTTCGACCACAACCGCTGGCTCTTCGAGACCAAGGGCACCTACGGCTACGGCAACGCAATCTGGCCCGAGGACGACGGCGTGGCGGGGCACCCCAAGGAGCTGATGAGCAAGCCATGGCGGCCGCTGACCCGCAAGCTCCGGATCCAGGCGGCGGTGATCAGCCCGTACAGGCTGCTGGTCCTGATCCGGCTGGTGGCGCTGGGGTTGTTCCTGATGTGGCGCATCAAGCACCAGAACGAGGACGCCATCTGGCTGTGGGGGATGTCCATCGTGTGCGAGCTGTGGTTCGCCTTGTCGTGGGTGCTGGACCAGCTGCCCAAGCTGTGCCCCATCAACCGCGCCACCGACCTGAGCGTGCTCAAGGACAAGTTCGAGACGCCCACGCCGAGCAACCCCACCGGCAAGTCCGACCTCCCCGGGATCGACATCTTCGTCTCCACCGCCGACCCGGAGAAGGAGCCCGTGCTGGTGACGGCCAACACCATCCTCTCCATCCTGGCCGCCGACTACCCCGTCGACAAGCTGGCGTGCTACGTGTCCGACGACGGCGGGGCGCTGCTGACGTTCGAGGCCATGGCGGAGGCGGCGAGCTTCGCCAACCTGTGGGTGCCATTCTGCCGGAAGCACGAGATCGAGCCGAGGAACCCCGACAGCTACTTCAACCTCAAGAGGGACCCGTTCAAGAACAAGGTGAAGGGCGACTTCGTCAAGGACAGGCGGCGGGTGAAGCGGGAGTACGACGAGTTCAAGGTCCGCGTCAATGGCCTCCCCGACGCCATCCGCCGCCGCTCCGACGCGTACCACGCCCGCGAGGAGATCCAGGCCATGAACCTGCAGCGGGAGAAGATGAAGGCCGGCGGCGACGAGCAGCAGCTGGAGCCGATAAAGATCCCCAAGGCGACGTGGATGGCGGACGGCACGCACTGGCCGGGGACGTGGCTGCAGGCGTCGCCGGAGCACGCGAGGGGCGACCACGCGGGGATCATACAGGTGATGCTAAAGCCTCCCAGTCCCAGTCCCAGCAGCAGCGGCGGCGACATGGAAAAGAGGGTGGATTTGTCGGGCGTGGACACCCGGCTGCCGATGCTGGTGTACGTGTCGCGGGAGAAGCGGCCGGGGTACGACCACAACAAGAAGGCGGGCGCCATGAACGCGCTGGTGCGGGCGTCGGCGATCATGTCCAACGGGCCGTTCATCCTCAACCTGGACTGCGACCACTACGTGTACAACTCCAAGGCGTTCCGGGAGGGGATGTGCTTCATGATGGACCGCGGCGGCGACCGCCTCTGCTACGTCCAGTTCCCGCAGCGCTTCGAGGGCATCGACCCCTCCGACCGCTACGCCAACCACAACACCGTCTTCTTCGACGTCAACATGCGCGCCCTCGACGGCCTCCAGGGCCCCGTCTATGTCGGAACTGGCTGCCTCTTCCGCCGCATCGCGCTCTACGGCTTCGACCCGCCCAGGTCCAAGGACCACACCACCCCGTGGTCCTGCTGCCTGCCACGTCGCCGCCGAACAAGATCCCAGCCCCAGCCCCAGGAGGAGGAGGAGGAGACGATGGCCCTCCGCATGGACATGGACGGCGCGATGAACATGGCGAGCTTCCCCAAGAAGTTCGGCAACTCGAGCTTCCTGATCGACTCCATCCCGGTGGCGGAGTTCCAGGGACGGCCGCTGGCGGACCACCCGTCGGTGAAGAACGGGCGGCCGCCGGGGGCGCTGACGATCCCGCGGGAGACGCTGGACGCGTCGATCGTGGCGGAGGCGATCAGCGTGGTGTCGTGCTGGTACGAGGAGAAGACGGAGTGGGGGACGAGGGTGGGTTGGATATACGGGTCGGTGACGGAGGACGTGGTCACCGGCTACCGCATGCACAACCGGGGGTGGAAGTCCGTCTACTGCGTCACCCACCGCGACGCCTTCCGCGGCACCGCCCCCATCAACCTCACCGACCGCCTCCACCAGGTCCTCCGATGGGCCACTGGCTCCGTCGAGATCTTCTTCTCACGCAACAACGCACTCTTCGCTTCCTCAAAGATGAAGGTTCTCCAGCGCATCGCTTACCTCAACGTTGGCATCTACCCCTTCACCTCCGTCTTCCTCATCGTCTACTGCTTCCTCCCGGCGCTCTCCCTCTTCTCGGGGCAGTTCATCGTGCAGACGCTCAACGTCACCTTCCTCACCTACCTGCTCATCATCACCATCACGCTCTGCCTCCTGGCCATGCTGGAGATCAAGTGGTCCGGCATCGCGCTGGAGGAGTGGTGGCGGAACGAGCAGTTCTGGCTCATCGGCGGAACCAGCGCCCACCTCGCCGCCGTCCTGCAGGGCCTTCTCAAGGTCATCGCCGGCATCGAGATCTCCTTCACCCTCACCTCCAAGCAGCTCGGCGACGACGTCGACGACGAGTTCGCCGAGCTCTACGCGGTCAAGTGGACGTCCCTCATGATACCGCCGCTCACCATCATAATGATCAACCTCGTCGCCATCGCCGTCGGCTTCAGCCGCACCATCTACAGCACCATCCCGCAGTGGAGCAAGCTTCTCGGTGGAGTCTTCTTCAGCTTCTGGGTCCTCGCGCATCTCTACCCCTTCGCCAAGGGCCTCATGGGCAGGAGGGGAAGGACACCCACCATCGTCTACGTCTGGTCTGGCCTCGTCGCCATCACCATCTCCTTGCTCTGGATTGCAATCAAGCCTCCTTCTGCACAAGCCAACTCACAGCTAGGAGGATCCTTCTCTTTCCCCTGA</cdnaseq>
Protein Sequence	<aaseq>MASKGILKNGGKPPTAPSSAAPTVVFGRRTDSGRFISYSRDDLDSEISSVDFQDYHVHIPMTPDNQPMDPAAGDEQQYVSSSLFTGGFNSVTRAHVMEKQASSARATVSACMVQGCGSKIMRNGRGADILPCECDFKICVDCFTDAVKGGGGVCPGCKEPYKHAEWEEVVSASNHDAINRALSLPHGHGHGPKMERRLSLVKQNGGAPGEFDHNRWLFETKGTYGYGNAIWPEDDGVAGHPKELMSKPWRPLTRKLRIQAAVISPYRLLVLIRLVALGLFLMWRIKHQNEDAIWLWGMSIVCELWFALSWVLDQLPKLCPINRATDLSVLKDKFETPTPSNPTGKSDLPGIDIFVSTADPEKEPVLVTANTILSILAADYPVDKLACYVSDDGGALLTFEAMAEAASFANLWVPFCRKHEIEPRNPDSYFNLKRDPFKNKVKGDFVKDRRRVKREYDEFKVRVNGLPDAIRRRSDAYHAREEIQAMNLQREKMKAGGDEQQLEPIKIPKATWMADGTHWPGTWLQASPEHARGDHAGIIQVMLKPPSPSPSSSGGDMEKRVDLSGVDTRLPMLVYVSREKRPGYDHNKKAGAMNALVRASAIMSNGPFILNLDCDHYVYNSKAFREGMCFMMDRGGDRLCYVQFPQRFEGIDPSDRYANHNTVFFDVNMRALDGLQGPVYVGTGCLFRRIALYGFDPPRSKDHTTPWSCCLPRRRRTRSQPQPQEEEEETMALRMDMDGAMNMASFPKKFGNSSFLIDSIPVAEFQGRPLADHPSVKNGRPPGALTIPRETLDASIVAEAISVVSCWYEEKTEWGTRVGWIYGSVTEDVVTGYRMHNRGWKSVYCVTHRDAFRGTAPINLTDRLHQVLRWATGSVEIFFSRNNALFASSKMKVLQRIAYLNVGIYPFTSVFLIVYCFLPALSLFSGQFIVQTLNVTFLTYLLIITITLCLLAMLEIKWSGIALEEWWRNEQFWLIGGTSAHLAAVLQGLLKVIAGIEISFTLTSKQLGDDVDDEFAELYAVKWTSLMIPPLTIIMINLVAIAVGFSRTIYSTIPQWSKLLGGVFFSFWVLAHLYPFAKGLMGRRGRTPTIVYVWSGLVAITISLLWIAIKPPSAQANSQLGGSFSFP</aaseq>
Gene Sequence	<dnaseqindica>1..2676#2767..3474#ATGGCGTCGAAGGGCATCCTCAAGAACGGCGGTAAGCCGCCGACGGCGCCATCGTCGGCGGCGCCCACGGTGGTGTTCGGTCGGCGGACGGACTCGGGGCGATTCATCAGCTACTCCCGCGACGACCTTGACTCGGAGATCAGCAGCGTGGACTTCCAGGACTACCACGTCCACATCCCCATGACGCCGGACAACCAGCCCATGGACCCTGCCGCCGGCGACGAGCAGCAGTACGTGTCCAGCTCGCTCTTCACCGGCGGGTTCAACAGCGTGACACGCGCGCACGTGATGGAGAAGCAGGCGTCGTCGGCGAGGGCGACGGTGTCGGCGTGCATGGTGCAGGGGTGCGGGTCCAAGATCATGCGCAACGGGCGCGGCGCCGACATCCTCCCCTGCGAGTGCGACTTCAAGATCTGCGTGGACTGCTTCACCGACGCCGTCAAGGGCGGCGGCGGCGTGTGCCCGGGGTGCAAGGAGCCGTACAAGCACGCGGAGTGGGAGGAGGTGGTGTCGGCGTCCAACCACGACGCCATCAACAGGGCGCTGTCGCTGCCGCACGGGCACGGGCATGGCCCCAAGATGGAGAGGCGGCTGTCGCTGGTGAAGCAGAACGGGGGGGCGCCCGGCGAGTTCGACCACAACCGCTGGCTCTTCGAGACCAAGGGCACCTACGGCTACGGCAACGCAATCTGGCCCGAGGACGACGGCGTGGCGGGGCACCCCAAGGAGCTGATGAGCAAGCCATGGCGGCCGCTGACCCGCAAGCTCCGGATCCAGGCGGCGGTGATCAGCCCGTACAGGCTGCTGGTCCTGATCCGGCTGGTGGCGCTGGGGTTGTTCCTGATGTGGCGCATCAAGCACCAGAACGAGGACGCCATCTGGCTGTGGGGGATGTCCATCGTGTGCGAGCTGTGGTTCGCCTTGTCGTGGGTGCTGGACCAGCTGCCCAAGCTGTGCCCCATCAACCGCGCCACCGACCTGAGCGTGCTCAAGGACAAGTTCGAGACGCCCACGCCGAGCAACCCCACCGGCAAGTCCGACCTCCCCGGGATCGACATCTTCGTCTCCACCGCCGACCCGGAGAAGGAGCCCGTGCTGGTGACGGCCAACACCATCCTCTCCATCCTGGCCGCCGACTACCCCGTCGACAAGCTGGCGTGCTACGTGTCCGACGACGGCGGGGCGCTGCTGACGTTCGAGGCCATGGCGGAGGCGGCGAGCTTCGCCAACCTGTGGGTGCCATTCTGCCGGAAGCACGAGATCGAGCCGAGGAACCCCGACAGCTACTTCAACCTCAAGAGGGACCCGTTCAAGAACAAGGTGAAGGGCGACTTCGTCAAGGACAGGCGGCGGGTGAAGCGGGAGTACGACGAGTTCAAGGTCCGCGTCAATGGCCTCCCCGACGCCATCCGCCGCCGCTCCGACGCGTACCACGCCCGCGAGGAGATCCAGGCCATGAACCTGCAGCGGGAGAAGATGAAGGCCGGCGGCGACGAGCAGCAGCTGGAGCCGATAAAGATCCCCAAGGCGACGTGGATGGCGGACGGCACGCACTGGCCGGGGACGTGGCTGCAGGCGTCGCCGGAGCACGCGAGGGGCGACCACGCGGGGATCATACAGGTGATGCTAAAGCCTCCCAGTCCCAGTCCCAGCAGCAGCGGCGGCGACATGGAAAAGAGGGTGGATTTGTCGGGCGTGGACACCCGGCTGCCGATGCTGGTGTACGTGTCGCGGGAGAAGCGGCCGGGGTACGACCACAACAAGAAGGCGGGCGCCATGAACGCGCTGGTGCGGGCGTCGGCGATCATGTCCAACGGGCCGTTCATCCTCAACCTGGACTGCGACCACTACGTGTACAACTCCAAGGCGTTCCGGGAGGGGATGTGCTTCATGATGGACCGCGGCGGCGACCGCCTCTGCTACGTCCAGTTCCCGCAGCGCTTCGAGGGCATCGACCCCTCCGACCGCTACGCCAACCACAACACCGTCTTCTTCGACGTCAACATGCGCGCCCTCGACGGCCTCCAGGGCCCCGTCTATGTCGGAACTGGCTGCCTCTTCCGCCGCATCGCGCTCTACGGCTTCGACCCGCCCAGGTCCAAGGACCACACCACCCCGTGGTCCTGCTGCCTGCCACGTCGCCGCCGAACAAGATCCCAGCCCCAGCCCCAGGAGGAGGAGGAGGAGACGATGGCCCTCCGCATGGACATGGACGGCGCGATGAACATGGCGAGCTTCCCCAAGAAGTTCGGCAACTCGAGCTTCCTGATCGACTCCATCCCGGTGGCGGAGTTCCAGGGACGGCCGCTGGCGGACCACCCGTCGGTGAAGAACGGGCGGCCGCCGGGGGCGCTGACGATCCCGCGGGAGACGCTGGACGCGTCGATCGTGGCGGAGGCGATCAGCGTGGTGTCGTGCTGGTACGAGGAGAAGACGGAGTGGGGGACGAGGGTGGGTTGGATATACGGGTCGGTGACGGAGGACGTGGTCACCGGCTACCGCATGCACAACCGGGGGTGGAAGTCCGTCTACTGCGTCACCCACCGCGACGCCTTCCGCGGCACCGCCCCCATCAACCTCACCGACCGCCTCCACCAGGTCCTCCGATGGGCCACTGGCTCCGTCGAGATCTTCTTCTCACGCAACAACGCACTCTTCGCTTCCTCAAAGATGAAGGTGCCTAGCTTGCTATTCGATCGATTATTTTTCTTCTTCTTCTTCTTCTTCCGCTGCCGCCAAACAAACAATTATCCATTGCATTTGCAGGTTCTCCAGCGCATCGCTTACCTCAACGTTGGCATCTACCCCTTCACCTCCGTCTTCCTCATCGTCTACTGCTTCCTCCCGGCGCTCTCCCTCTTCTCGGGGCAGTTCATCGTGCAGACGCTCAACGTCACCTTCCTCACCTACCTGCTCATCATCACCATCACGCTCTGCCTCCTGGCCATGCTGGAGATCAAGTGGTCCGGCATCGCGCTGGAGGAGTGGTGGCGGAACGAGCAGTTCTGGCTCATCGGCGGAACCAGCGCCCACCTCGCCGCCGTCCTGCAGGGCCTTCTCAAGGTCATCGCCGGCATCGAGATCTCCTTCACCCTCACCTCCAAGCAGCTCGGCGACGACGTCGACGACGAGTTCGCCGAGCTCTACGCGGTCAAGTGGACGTCCCTCATGATACCGCCGCTCACCATCATAATGATCAACCTCGTCGCCATCGCCGTCGGCTTCAGCCGCACCATCTACAGCACCATCCCGCAGTGGAGCAAGCTTCTCGGTGGAGTCTTCTTCAGCTTCTGGGTCCTCGCGCATCTCTACCCCTTCGCCAAGGGCCTCATGGGCAGGAGGGGAAGGACACCCACCATCGTCTACGTCTGGTCTGGCCTCGTCGCCATCACCATCTCCTTGCTCTGGATTGCAATCAAGCCTCCTTCTGCACAAGCCAACTCACAGCTAGGAGGATCCTTCTCTTTCCCCTGA</dnaseqindica>
External Link(s)	EnsemblPlants:BGIOSGA033504