Difference between revisions of "Os10g0467800"
(→Function: The gene encodes a essential subunit which is required for the correct assembly of the enzyme responsible for cellulose synthesis.) |
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===Expression=== | ===Expression=== | ||
| − | + | OsCesA7 cDNA, including 9 exons, encoding a 1063 amino acid composition of cellulose synthase catalytic subunit. OsCesA7 contains a UDP - Glc motif, QxxRW motif, P - CR conservative domain, VR1, VR2 variable region and the RING finger motif. The rsw1mutation in AtCesA1 causes a reduction of cellulose synthesis, when grown at the nonpermissive temperature, resulting in disassembly of the rosette complexes, widespread morphological abnormalities, and the accumulation of noncrystalline β-1,4-glucan. The irx3 mutant of AtCesA7 shows a reduction of cellulose content in the stem and a defect in the secondary cell wall formation in xylem that causes collapse of xylem elements. These results indicate that AtCesA1 and AtCesA7 contribute to cellulose syntheses in the properly developed primary and secondary cell wall, respectively. | |
| + | |||
| + | Based on the sequences shown in Figure 3, phylogenetic relationships of CesA genes from higher plants were examined (Fig. 4). Three rice CesA genes were considered to function to synthesize cellulose in the secondary cell walls responsible for the overall strength of the plant, judging from the brittle culm phenotype (Fig. 1) exhibited by their respective mutants (Fig. 2). | ||
| + | |||
| + | The phylogenetic relationships between the three rice CesA genes and all of the Arabidopsis genes indicated that OsCesA9, OsCesA4, and OsCesA7 are the functional analogues for AtCesA7, AtCesA8, and AtCesA4, respectively. This result strongly suggests that these three rice CESA proteins, like the three Arabidopsis CESA proteins, make a complex essential for cellulose synthesis in secondary cell wall. Two cotton CesA genes (GhCesA1 and -3) that are considered to be members of secondary wall-forming genes (Pear et al., 1996; Peng et al., 2002) belong to the same group including OsCesA4 and AtCesA8. GhCesA1 and -3 may have been evolved by recent gene duplication. In maize, only eight complete amino acid sequences have been determined, and no CesA genes analogous to the three rice/Arabidopsis genes have been isolated, probably due to low abundance of the cDNAs. | ||
===Evolution=== | ===Evolution=== | ||
Revision as of 08:56, 3 June 2014
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Contents
Annotated Information
Function
Cellulose is the major component in wood, which is present universally in plant cell walls and provides mechanical strength to the plant. Cellulose accounts for about 20 % and 50 % of the primary and secondary cell walls in higher plants, respectively. Membrane-bound cellulose synthase enzyme complexes (CelS) are believed to play a key role in the biosynthesis of cellulose. These complexes are discernible as hexameric rosettes under freeze-fracture electron microscopy and consist of 36 cellulose synthase (CesA) proteins per rosette.The AtCesA7 gene encodes a essential subunit which is required for the correct assembly of the enzyme responsible for cellulose synthesis. Cellulose is a homogenous polymer of β-1,4-glucan synthesized from UDP-Glc. Plant CesA (cellulose synthase catalytic subunit) genes were first isolated by random sequencing of cotton (Gossypium hirsutum) expressed sequence tags . Based on mutant analyses, characterization of six CesA genes of Arabidopsis has been reported. But cellulose biogenesis is a complex process, which still requires ongoing study to comprehend completely its pathways and mechanisms. Even with the completion of the Arabidopsis thaliana genome sequencing project, researchers still struggle to understand the biogenesis of cellulose in A. thaliana，let alone other plants that are less well studied.
Expression
OsCesA7 cDNA, including 9 exons, encoding a 1063 amino acid composition of cellulose synthase catalytic subunit. OsCesA7 contains a UDP - Glc motif, QxxRW motif, P - CR conservative domain, VR1, VR2 variable region and the RING finger motif. The rsw1mutation in AtCesA1 causes a reduction of cellulose synthesis, when grown at the nonpermissive temperature, resulting in disassembly of the rosette complexes, widespread morphological abnormalities, and the accumulation of noncrystalline β-1,4-glucan. The irx3 mutant of AtCesA7 shows a reduction of cellulose content in the stem and a defect in the secondary cell wall formation in xylem that causes collapse of xylem elements. These results indicate that AtCesA1 and AtCesA7 contribute to cellulose syntheses in the properly developed primary and secondary cell wall, respectively.
Based on the sequences shown in Figure 3, phylogenetic relationships of CesA genes from higher plants were examined (Fig. 4). Three rice CesA genes were considered to function to synthesize cellulose in the secondary cell walls responsible for the overall strength of the plant, judging from the brittle culm phenotype (Fig. 1) exhibited by their respective mutants (Fig. 2).
The phylogenetic relationships between the three rice CesA genes and all of the Arabidopsis genes indicated that OsCesA9, OsCesA4, and OsCesA7 are the functional analogues for AtCesA7, AtCesA8, and AtCesA4, respectively. This result strongly suggests that these three rice CESA proteins, like the three Arabidopsis CESA proteins, make a complex essential for cellulose synthesis in secondary cell wall. Two cotton CesA genes (GhCesA1 and -3) that are considered to be members of secondary wall-forming genes (Pear et al., 1996; Peng et al., 2002) belong to the same group including OsCesA4 and AtCesA8. GhCesA1 and -3 may have been evolved by recent gene duplication. In maize, only eight complete amino acid sequences have been determined, and no CesA genes analogous to the three rice/Arabidopsis genes have been isolated, probably due to low abundance of the cDNAs.
Evolution
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Labs working on this gene
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References
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Structured Information
| Gene Name |
Os10g0467800 |
|---|---|
| Description |
Similar to Cellulose synthase (Fragment) |
| Version |
NM_001071346.1 GI:115482435 GeneID:4348853 |
| Length |
4512 bp |
| Definition |
Oryza sativa Japonica Group Os10g0467800, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 10:17719262..17723773 |
| Sequence Coding Region |
17719294..17719507,17719609..17719736,17719852..17719921,17720009..17720165,17720266..17720878 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008403:17719262..17723773 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008403:17719262..17723773 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggacaccgcctccgtcaccggtggcgagcacaaggggaaggagaagacgtgccgggtgtgcggcgaggaggtggcggcgagggaggacgggaagccgttcgtggcgtgcgccgagtgcggcttcccggtgtgcaagccctgctacgagtacgagcgcagcgagggcacccagtgctgcccccagtgcaacacccgctacaagcgccacaaagggtgcccacgggtggaaggcgacgaggacgacggcggcgacatggacgacttcgaggaggagttccagatcaagagccccaccaagcagaaacccccccacgagcccgtcaacttcgacgtctactcggagaacggcgagcagccggcacagaagtggcgccctggaggcccggcgctctcttccttcaccggaagcgtggctgggaaggatctggagcaggagagggagatggagggtggcatggagtggaaggacaggatcgacaagtggaagacgaagcaggagaagcggggcaagctcaaccgcgacgacagcgacgacgacgacgacaagaacgacgacgagtacatgctgctcgcggaggcgaggcagccgctgtggaggaaggtgccgatcccgtcgagcaagatcaacccgtaccggatcgtgatcgtgctccggctggtggtgctctgcttcttcctcaagttccggatcacgacgccggcgatggacgcggtgccgctgtggctggcctcggtgatctgcgagctgtggttcgcgctgtcgtggatcctcgaccagctgcccaagtggtcgccggtgacgagggagacgtacctggaccggctggccctccggtacgagcgcgacggcgagccgtgccgcctggccccgatcgatttcttcgtcagcacggtggacccgctcaaggagccgcccatcatcaccgccaacaccgtgctgtccatcctcgccgtcgactaccccgtcgaccgcgtctcctgctacgtctccgacgacggcgcgtccatgctgctcttcgacacgctctccgagaccgccgagttcgcccgccggtgggtccccttctgcaagaagttcaccatcgagccccgcgcccccgagttctacttctcccagaagatcgactacctcaaggacaaggtccagcccaccttcgtcaaagaacgccgcgccatgaagagagagtatgaggagttcaaggtgaggataaacgcgctggtggcgaaggcgcagaagaagccggaggaagggtgggtgatgcaggacgggacgccatggccggggaacaacacgagggaccacccggggatgatccaggtgtacctgggcagccagggcgcgctcgacgtcgagggcagcgagctgccgcggctggtgtacgtgtcccgcgagaagcggcccggctacaaccaccacaagaaggccggcgccatgaactccctcgttcgcgtctccgccgtgcttaccaacgcccccttcatcctcaacctcgactgcgaccactacgtcaacaacagcaaggccgtccgcgaggccatgtgcttcctcatggacaagcagctcggcaagaagctgtgctacgtccagttcccccagcgcttcgacggcatcgaccgccacgatcgctacgccaaccgcaacaccgtcttcttcgacatcaacatgaaggggctggacgggatacaggggccggtgtacgtggggacggggacggtgttcaacaggcaggcgctgtacggatacgacccgccgcggccggagaagaggccgaagatgacgtgcgactgctggccgtcgtggtgctgctgctgctgctgcttcggcggggggaagcgcggcaagtcgcacaagaacaagaagggcggcggcggcggcgagggcggcggcctcgacgagccgcgccgcgggctgctcgggttctacaagaagaggagcaagaaggacaagctcggcggcggcgcggcgtcgctcgccggagggaagaaagggtaccggaagcaccagcgcgggttcgagctggaggagatcgaggagggcctcgaggggtacgacgagctggagcgctcgtcgctcatgtcgcagaagagcttcgagaagcggttcggccagtcgccggtgttcatcgcctccaccctcgtcgaggacggcggcctcccccagggcgccgccgccgaccccgccgccctcatcaaggaggccatccacgtcatcagctgcggctacgaggagaagaccgagtggggcaaggagattgggtggatctacgggtcggtgacggaggacatcttaacggggttcaagatgcattgccgtgggtggaagtcggtgtactgcacgccggcgagggcggcattcaaggggtcggcgcccatcaacctgtcggatcgtctgcaccaggtgctccggtgggcgctcggctccgtcgagatcttcatgagccgccattgcccgctctggtacgcctatggcggccgcctcaagtggctcgagcgcttcgcctacaccaacaccatcgtctaccccttcacctccattcccctcctcgcctactgcaccatccccgccgtctgcctcctcaccggcaagttcatcatccccacgcttaacaatttggcgagcatatggttcatagcgcttttcctgtcgatcatcgcgacgggggtgctggagctgcggtggagcggggtgagcatcgaggactggtggaggaacgagcagttctgggtgatcggcggcgtgtcggcgcacctgttcgccgtgttccagggcctcctcaaggtgctcggcggcgtggacaccaacttcacggtgacgtccaaggccgccgccgacgagaccgacgcgttcggcgagctctacctgttcaagtggacgacgctgctggtgccgccgacgacgctgatcatcatcaacatggtggggatcgtcgccggcgtgtcggacgccgtgaacaacgggtacgggtcgtggggcccgctgttcgggaagctcttcttctccttctgggtcatcctccacctctaccccttcctcaaggggctcatggggaggcagaaccggacgcccaccattgtcgtgctctggtccatcctcctcgcctccatcttctccctcgtctgggtcaggatcgaccccttcatccccaagcccaagggccccgtcctcaagccatgcggggtctcgtgctga</cdnaseq> |
| Protein Sequence |
<aaseq>MDTASVTGGEHKGKEKTCRVCGEEVAAREDGKPFVACAECGFPV CKPCYEYERSEGTQCCPQCNTRYKRHKGCPRVEGDEDDGGDMDDFEEEFQIKSPTKQK PPHEPVNFDVYSENGEQPAQKWRPGGPALSSFTGSVAGKDLEQEREMEGGMEWKDRID KWKTKQEKRGKLNRDDSDDDDDKNDDEYMLLAEARQPLWRKVPIPSSKINPYRIVIVL RLVVLCFFLKFRITTPAMDAVPLWLASVICELWFALSWILDQLPKWSPVTRETYLDRL ALRYERDGEPCRLAPIDFFVSTVDPLKEPPIITANTVLSILAVDYPVDRVSCYVSDDG ASMLLFDTLSETAEFARRWVPFCKKFTIEPRAPEFYFSQKIDYLKDKVQPTFVKERRA MKREYEEFKVRINALVAKAQKKPEEGWVMQDGTPWPGNNTRDHPGMIQVYLGSQGALD VEGSELPRLVYVSREKRPGYNHHKKAGAMNSLVRVSAVLTNAPFILNLDCDHYVNNSK AVREAMCFLMDKQLGKKLCYVQFPQRFDGIDRHDRYANRNTVFFDINMKGLDGIQGPV YVGTGTVFNRQALYGYDPPRPEKRPKMTCDCWPSWCCCCCCFGGGKRGKSHKNKKGGG GGEGGGLDEPRRGLLGFYKKRSKKDKLGGGAASLAGGKKGYRKHQRGFELEEIEEGLE GYDELERSSLMSQKSFEKRFGQSPVFIASTLVEDGGLPQGAAADPAALIKEAIHVISC GYEEKTEWGKEIGWIYGSVTEDILTGFKMHCRGWKSVYCTPARAAFKGSAPINLSDRL HQVLRWALGSVEIFMSRHCPLWYAYGGRLKWLERFAYTNTIVYPFTSIPLLAYCTIPA VCLLTGKFIIPTLNNLASIWFIALFLSIIATGVLELRWSGVSIEDWWRNEQFWVIGGV SAHLFAVFQGLLKVLGGVDTNFTVTSKAAADETDAFGELYLFKWTTLLVPPTTLIIIN MVGIVAGVSDAVNNGYGSWGPLFGKLFFSFWVILHLYPFLKGLMGRQNRTPTIVVLWS ILLASIFSLVWVRIDPFIPKPKGPVLKPCGVSC</aaseq> |
| Gene Sequence |
<dnaseqindica>33..246#348..475#591..660#748..904#1005..1617#2048..2524#2602..3195#3275..3625#3724..4311#gcccaccaacgtcgcttccccggcacctcaccatggacaccgcctccgtcaccggtggcgagcacaaggggaaggagaagacgtgccgggtgtgcggcgaggaggtggcggcgagggaggacgggaagccgttcgtggcgtgcgccgagtgcggcttcccggtgtgcaagccctgctacgagtacgagcgcagcgagggcacccagtgctgcccccagtgcaacacccgctacaagcgccacaaaggtaacatcatgtgttgtgtccatgtgatcaatcgcaatttagttcacaagatcgagcttgacaagaatgtctgtctgtctgtctcgatcgatcggtcgcagggtgcccacgggtggaaggcgacgaggacgacggcggcgacatggacgacttcgaggaggagttccagatcaagagccccaccaagcagaaacccccccacgagcccgtcaacttcgacgtctactcggtgagagctcaactcgactcgatcgatccatcgccattgatttctcactgcaattgctctgcaatcaagtgcttgtaaactgatgcgaaacctgtatacaacctttgtcttgcaggagaacggcgagcagccggcacagaagtggcgccctggaggcccggcgctctcttccttcaccggaagcggtcggtactcaaatcccgcaagaaattgcgtttcttggtgtaacaattcatctgaagatgtgtgtgtggattgctgtgaaattgcagtggctgggaaggatctggagcaggagagggagatggagggtggcatggagtggaaggacaggatcgacaagtggaagacgaagcaggagaagcggggcaagctcaaccgcgacgacagcgacgacgacgacgacaagaacgacgacgagtacatgctgtaagcttagctctccaaagatctatctatcgatcgacctcagatttttcaaatcttgtgaactttggcatgatcgtgatgatcgaatttctgcatgcaggctcgcggaggcgaggcagccgctgtggaggaaggtgccgatcccgtcgagcaagatcaacccgtaccggatcgtgatcgtgctccggctggtggtgctctgcttcttcctcaagttccggatcacgacgccggcgatggacgcggtgccgctgtggctggcctcggtgatctgcgagctgtggttcgcgctgtcgtggatcctcgaccagctgcccaagtggtcgccggtgacgagggagacgtacctggaccggctggccctccggtacgagcgcgacggcgagccgtgccgcctggccccgatcgatttcttcgtcagcacggtggacccgctcaaggagccgcccatcatcaccgccaacaccgtgctgtccatcctcgccgtcgactaccccgtcgaccgcgtctcctgctacgtctccgacgacggcgcgtccatgctgctcttcgacacgctctccgagaccgccgagttcgcccgccggtgggtccccttctgcaagaagttcaccatcgagccccgcgcccccgagttctacttctcccagaagatcgactacctcaaggacaaggtccagcccaccttcgtcaaagaacgccgcgccatgaaggtaaaccttctccactccatctgcgggttgagtgtcgtggatataccggattcaagggtttaatatattgttttaggcatttgtgttggggggaccgatatagccggttatttcgatccaaaattattttattttattttgaaaaaaaatgattaaaatttgaataaatattatcaaattcacaaaaatttgaaaaacatttaaaagtttcgtccgaaataatatcatatcaggttgtccaatagaactgaaatgtcaaaaattatgttatgtaattttgaaccctcattcaattggagcatctcgtgatcagtctgtttttggagatgtaggctatggctaaacttcgttaccgatatatcccttctcgatgttgtcatccagtttagatcgtctataagttttttaacacctactcttgatgtgacagagagagtatgaggagttcaaggtgaggataaacgcgctggtggcgaaggcgcagaagaagccggaggaagggtgggtgatgcaggacgggacgccatggccggggaacaacacgagggaccacccggggatgatccaggtgtacctgggcagccagggcgcgctcgacgtcgagggcagcgagctgccgcggctggtgtacgtgtcccgcgagaagcggcccggctacaaccaccacaagaaggccggcgccatgaactccctcgttcgcgtctccgccgtgcttaccaacgcccccttcatcctcaacctcgactgcgaccactacgtcaacaacagcaaggccgtccgcgaggccatgtgcttcctcatggacaagcagctcggcaagaagctgtgctacgtccagttcccccagcgcttcgacggcatcgaccgccacgatcgctacgccaaccgcaacaccgtcttcttcgacgtgagcactgccttcttctccggcgtgcgtgcgtcgcgccgtgacgtgagctaacgatggtgtcgtttgatgtgcagatcaacatgaaggggctggacgggatacaggggccggtgtacgtggggacggggacggtgttcaacaggcaggcgctgtacggatacgacccgccgcggccggagaagaggccgaagatgacgtgcgactgctggccgtcgtggtgctgctgctgctgctgcttcggcggggggaagcgcggcaagtcgcacaagaacaagaagggcggcggcggcggcgagggcggcggcctcgacgagccgcgccgcgggctgctcgggttctacaagaagaggagcaagaaggacaagctcggcggcggcgcggcgtcgctcgccggagggaagaaagggtaccggaagcaccagcgcgggttcgagctggaggagatcgaggagggcctcgaggggtacgacgagctggagcgctcgtcgctcatgtcgcagaagagcttcgagaagcggttcggccagtcgccggtgttcatcgcctccaccctcgtcgaggacggcggcctcccccagggcgccgccgccgaccccgccgccctcatcaaggaggccatccacgtcatcagctgcggctacgaggagaagaccgagtggggcaaggaggtaatccatcaaaaaatctccaccattttcaagctcgaggaagatgatcgaagctaaccaaaaatcaaaattcttgcagattgggtggatctacgggtcggtgacggaggacatcttaacggggttcaagatgcattgccgtgggtggaagtcggtgtactgcacgccggcgagggcggcattcaaggggtcggcgcccatcaacctgtcggatcgtctgcaccaggtgctccggtgggcgctcggctccgtcgagatcttcatgagccgccattgcccgctctggtacgcctatggcggccgcctcaagtggctcgagcgcttcgcctacaccaacaccatcgtctaccccttcacctccattcccctcctcgcctactgcaccatccccgccgtctgcctcctcaccggcaagttcatcatccccacggtaaagaaccaaccaagaacatcaaaatttccgctaaaatttcaagctccaaatgatcttgcactgaccgatcgatcgcgatcttggtgtacgtgtagcttaacaatttggcgagcatatggttcatagcgcttttcctgtcgatcatcgcgacgggggtgctggagctgcggtggagcggggtgagcatcgaggactggtggaggaacgagcagttctgggtgatcggcggcgtgtcggcgcacctgttcgccgtgttccagggcctcctcaaggtgctcggcggcgtggacaccaacttcacggtgacgtccaaggccgccgccgacgagaccgacgcgttcggcgagctctacctgttcaagtggacgacgctgctggtgccgccgacgacgctgatcatcatcaacatggtggggatcgtcgccggcgtgtcggacgccgtgaacaacgggtacgggtcgtggggcccgctgttcgggaagctcttcttctccttctgggtcatcctccacctctaccccttcctcaaggggctcatggggaggcagaaccggacgcccaccattgtcgtgctctggtccatcctcctcgcctccatcttctccctcgtctgggtcaggatcgaccccttcatccccaagcccaagggccccgtcctcaagccatgcggggtctcgtgctgagctgctgctgctacttctctgtgtctctgcattttgcaagagggatgaccggatggatgattcttgttgtatggagtattttgacttgttcatgtacaagtttttgtgagtgggataaaagtgttttgggggtaaaatttgtaagaactgaggtggagattatactcgaatttaagaacaattgtttttgaattttctttt</dnaseqindica> |
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