Os10g0404500

Please input one-sentence summary here.

Annotated Information

Function

Plant sucrose transporters (SUTs) mediate the active transport of sucrose across plasma membrane barriers in a process that is coupled to proton symport. Since sucrose is the major carbohydrate translocated through the phloem in most plant species, the sucrose/H+ symporters are thought to play important roles in mediating carbon partitioning in plants, for example apoplastic phloem loading in leaves, transport of sucrose into and/or out of temporary storage sinks such as stem tissue and post-phloem transport of sucrose into sink tissue such as seeds.

The rice has five families of sucrose transporters. Despite of the previously reported OsSUT1, four putative sucrose transporter are known as OsSUT2, 3, 4 and 5. OsSUT1 encodes a functional SUT protein that is essential for transport of assimilate into filling rice grains. It has also been proposed that OsSUT1 is involved in transport of assimilate remobilised from starch reserves in leaf sheaths and in germinating seeds. Expression of OsSUT3, 4 and 5 in sink rice leaf suggests that they may be important for supplying sucrose, as a carbon source for growing tissues or possibly to supply sucrose to temporary storage tissues.Unlike the other four OsSUT genes, OsSUT2 seems to be expressed at almost equal levels in various tissues of rice plants.

Expression

The five families all contained a region that is highly conserved in known functional plant SUT genes, including OsSUT1. This domain includes the first membrane spanning helix, the following extracellular loop, the second membrane spanning helix and the next cytoplasmic loop have shown, by site-directed mutagenesis of the Arabidopsis AtSUC1 protein, that a conserved histidine residue in the extracellular loop is responsible for sucrose binding in the transport process. This histidine residue was also found to be present in all of the putative OsSUT peptides.

" The functionally important and conserved histidine residue is shown in bold. Dots indicate non-conserved amino acids, and horizontal bars indicate gaps in the sequence alignments(from reference ).. "

OsSUT1 mRNA accumulated to high levels in germinating seeds, source leaf sheaths and panicles, but to very low level in roots. OsSUT2 mRNA accumulated to nearly equal levels in all tissues tested. The expression patterns of OsSUT3 and 5 were found to be similar, the expression level is at its highest in sink leaves and the lowest in germinating seeds. OsSUT4 showed preferential expression in sink leaves.

" Analysis of expression of the five OsSUT genes, by semi-quantitative RT-PCR. For each gene, transcript levels in different tissue samples are comparable(from reference ).. "

Evolution

We can express the OsSUT gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.

" Test function in yeast(from reference ).. "

There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.

" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference ).. "

Labs working on this gene

Please input related labs here.

References

<references> 3.Lu J M-Y. and Bush D R.(1998) His-65 in the proton-sucrose symporter is an essential amino acid whose modification with site-directed mutagenesis increases transport activity. Proc Natl Acad 95: 9025–9030.</ref> 4.Furbank R T, Scofield G N, Hirose T, et al. (2001) Cellular localisation and function of a sucrose transporter OsSUT1 in developing rice grains. Aust. J. Plant Physiol 28: 1187–1196.</ref> 5.Hirose T, Imaizumi N, Scofield G N, et al. (1997) cDNA cloning and tissue-specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol 38: 1389–1396.</ref>

Structured Information

Gene Name	Os10g0404500
Description	Sucrose transporter
Version	NM_001071090.1 GI:115481923 GeneID:4348577
Length	6221 bp
Definition	Oryza sativa Japonica Group Os10g0404500, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 10
Location	Chromosome 10:14136198..14142418
Sequence Coding Region	14136282..14136437,14138096..14138161,14138312..14138410,14139725..14139789,14139915..14139999 ,14140094..14140127,14140244..14140343,14140516..14140591,14140790..14141113 ,14141795..14142310
Expression	GEO Profiles:Os10g0404500
Genome Context	<gbrowseImage1> name=NC_008403:14136198..14142418 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008403:14136198..14142418 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaaggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcagggtccagctcgcgctctgatggccgatttgtcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcgatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactga</cdnaseq>
Protein Sequence	<aaseq>MAVDMELDGGGDGKGKAPPQISLSGLFLACMVAGGVQYGWALQL SLLTPYVQTLGIPHALTSVMWLCGPIAGLIVQPCVGLYSDKCTSSLGRRRPFILTGCI IICISVIVIGFSSDIGYALGDTTEDCKVYRGPRYHAAAAFILGFWLLDFSNNTVQGPA RALMADLSGRHGPSAANAIFCSWMALGNILGYSSGSTNDWHKWFPFLMTRACCEACAN LKAAFLVAVVFLGLSTAVTMVFAREVALDPVAAAKRNEGEASGLLAVFKGMKNLPVGM PSVLIVTGLTWLSWFPFILFDTDWMGREIYHGRPDGSPAEVTAFQEGVRQGAFGLLLN SIVLGISSFLIEPMCRRLGARAVWVMSSAVVCVAMAAVSVLSAWSLGDFGGSVQDAAR APAEEGGVRASALALFVFLGLPFAVLCSVPFAVTAQLAASRGGGQGLCTGVLNISIVV PQMAIALGAGPWDELFGEGNIPAFAMASVFAAAAAAAGVVLLPKVSVRSVSMAGGH</aaseq>
Gene Sequence	<dnaseqindica>85..240#1899..1964#2115..2213#3528..3592#3718..3802#3897..3930#4047..4146#4319..4394#4593..4916#5598..6113#cttcgatctcttgggatataactagcttagctatagctctagctaaataggttcgtacgtataggatatttgatccattgatcgatggccgtcgacatggagctcgacggcggtggcgacggcaagggcaaagccccgccgcagataagcctgtcggggctattccttgcgtgcatggtcgccggtggcgtgcagtacggctgggcgctgcagctctctctcctcaccccgtacgttcaggtatgtgtttaaggcgttttggcctttttcattttcgtctatattcattagcatctatatgaatttgaacacacacacacacacacatatatatatatatatatatatatatatatatatatatatatatatatggattcacgtatgaatctattcaaaacccaaactgtcttaactaataatatggaacagagaacatagtactacttagttgttgtgattgtatatatgtactatacttttcttgtctccaagagggagatttttttttctattgtcttttaactctttattgatgtgaaggtgtaatttgtttgtgtggaaatatttggaggaattccaaatagtattgggaatatcagatttcctcttatgctgcctgtttgctatggctaatcttgatctaactcccacctcaaaaaaaaaacatgtagcttaacatctagctttcatattagcatcctcccaattaagctggttttagctatagcctattttatggtgtttttactcattatgttcctttggaaagcaacaatataattgttttcctcattcttgtagagttgaattaattcctgtgaaaatctgaaaaaaattgtgtgtacaattccagcatttctaaacaaaagcttgaacatttgctaatatacagttttcaaaagaaaaattttggaactggatttcacgattcatttttccaaggcacttgtcctgcaaaaagttctatagctgtaatatgggcaattccttgagtaataagtcatttatttcaccgcaagttcttgcgtagttgtggtgtaggtcagattgtttttatccatgaacttattacttctgtaaattaatttggtatatattgttaagttcagctatatcctggtaatagaaaatgaatgtttttattagtaaaagttttttttataaaatgaaatgaattcatttctatgtttaggagtaaagcattgtctagcacagcaagggactgtaactaaaaatggtatgtgagttgtttcatttttgttctaactgaaccatggcaccatataacaaagtattaatcttgaataaagtcaccagatgttattttttggtcaaaataaaaaggcattttccatatatttacaatcatactaccaaattaaaattatactccctccggtttttaatgtaccacgtcattaacttttagacatatgtttgaactttttgtgttattaaaaaaataatgcaaatacgtacatattatcaagattgcggtagattcagatcaattttcttgtgtggcttgttttggcctaggagtacacggactaaccaagttattgtatcaaaatgaacaacctacaagttgttgtatctatttgcacccaccacgcaagttcatgtaccatgagcacaatttactccttatattatctttaatataaaacaaatcaaaacaaaataaatatttattacataattcttttaataagacaaatgatcaaacatcgtccaaaagtcaacagcgtcgtacatgcatggaaaacctgagggagtacccttgttgtgctttgataaaatccagcatgaactaattactaatttctcttcatatatgtgcatggcttaattcattcactaacttcatttcactatcactttgtttggaacagacattgggaattcctcatgcactcacttcagttatgtggctctgtggccctattgctggcttaattgtaagtagtgccctatctatggcgatgtcatatatcattccctggtcttaaaaaaagaaaactcacaaaattatatcaagatttatgatttcagaaacatgatccctcaaagtacgttttctcctcacgtttgtgcctttcatcctgcaggttcagccatgcgtcggcctgtacagtgacaagtgcacatccagccttggaagacgtagaccgttcatcctcactggatgcataatcatctgcatatctgtgagaaaaacccaattatcatccaactactctcttcgtttcatattataaatcgtttaaacttttttttaagttaaacttgttttaaaaaattttgatcatcaaatgtatataaaaatatagtaatatttatagcaccaatttgcactacattcccagtgtcctgcaaccaaattgatagagagatattcgcggcagccacgaccatcaccctatgagatagaaagacggcgagtttctgcactcgcacaggcttgaaaacaagattcctaagacaatggcaccggccatctacgcatcttcaaggtgaaagaatcaaatagtttgcgaggccctctcctctaacactttgattcgtgacatctgactgctacaaatcacaacagtagatcacctaagtcagtatgtcgagctatggaccatgatccagagaataggccccttaggatactagagagacaccatcacttagaagtttacagcaaatggtgaatagtcggcaaaatcggcttatcagctacaattcctatgtgcagtaggaacggtgttcaatcaaacaatttggaaatgttaggcgcccctaaagtgcaaatttttcatatagttggtcatccaaaatagggtgtggacggctgataggctatagaagagaggttagcaaaaccaaagagtctgccccttctatagagtatcacaggaaacgacaatccatcttatcgcaaaatgcaggctctctataaggatttagacagccatacaacattgggcgagcatagacctcaaaatacaagattgggacgcttgcgcttcgatggaagaatggtggaagagggttcttcacacaccgcacatccccaaaggacctttaaaatccctgattatcctggtgggttgggagctctggtgtgagagaaacactaggatattccgccacgtggctaccaccctgacaaccatcatcgctaaaatcaaagaagaggggttggcctggatcaaagtgggatcaaagtgggagccgcaaaagctcgttgagttaacttcgttggaagaaccctatagtttctccttctgtgggctctttttttttatggcaggccctgtaaataactctttttttggttgtttgttttttctcctactctatcaatatatgcaaggtaaagctttcgtctttttttttaaaaaaaaagtgcactatatctaacaattcataaatttagttaaaactaaagtgacttaaaagttaaaaaaactaataatatgaaacggaggtaagtagttagttatttgattaactaattacatgactgacaggtgatcgtcatcgggttctcctcggacatcggctacgctctcggcgacacgactgaggactgcaagtaattaagctgctcgatcactgacagtgacagagcttgatgaattcgctgattaaatcattggtcgattagtaagcaaaatttcaggattcggactcgctaatcgagcgacttaatccatccagggtttacaggggtcctcgttaccatgcagcggcggcattcatcctcggattctggctgctcgacttctccaacaacaccgtgcaggtatgcagcctgaagaagaagaaagaaaactctcagctctgaatccatctgaaactctgaataatctgcaatgtgatatatgattctcgcgcagggtccagctcgcgctctgatggccgatttgtcaggtgagtcgtgagaagattaacaccagattttatacctgatttgatgacagattagttgtgttcttggttttggttttctgaattttgttgcttactgaacattggaatatattcaggtcggcatggcccaagcgcggccaacgcgatcttctgttcttggatggcattaggcaacatccttggctactcctccggatccaccaatgactggcacaagtcggtgatcgatcgctgatgactacagctgctctgaattttctcatcatcaaaatcatctatggaatgctaaatcagcagctttgatcagttcttgatgccaatttttttttctaagaaaaatgaacggaatgctatattcatagtggaattgcattgcatgtggccgcaggtggtttccctttctcatgaccagggcttgctgcgaggcctgcgcgaatctcaaagcggccttcttggttgcagtggtaattattttatttaattaaaagctgcagcttttatacaactatagtaccttcaaaattgaaggtttttacgtgacatgtatttttttctattacacgaaagacgatgcgtaatgcatcatggaacacacattcaagacaagaccagcgacaaaatctctaaaattactaaatttgattttgatgtaatgtgcgcaggtgtttttggggttgtcgacggcggtgacgatggtgtttgcgagggaggtggcgctggacccagtggcggcggcgaagcggaacgagggtgaggcgtcggggctcctcgccgtgttcaaggggatgaagaacctccccgtcgggatgccgtcggtgcttatcgtcaccggcctcacctggctctcgtggttccccttcatcctcttcgacaccgactggatgggccgcgagatctaccatggccgcccggacggctcccccgccgaggtcaccgccttccaagagggtgtccgccaaggcgccttcggcctcctccttaactcggtcagatcgagctatccatcaattacttgcctttttttctctccggtttaaaatatttgatgttaagaataatatttaaattattttttaaaaaataaaattgacctaaaacctaaagttttgaattgtgatagtgttaatacttttcgaggcaaatatgcacataaaagttttctttttctacttttttaactaacaatttgaaaaatgatttaactgtcaagttttcaaaatcctgatcaaatcttttccctgaacgtctattattttcttttacggagactgtatctactagctacttcattccacgttataataataagacgttatgatatcaccgttactcgtatagattcactatctttcatatgaatctaaacacatatataatacacacgaattaatctatacgttaatctttactacttaaaacaatgaagatgtttcttcgtccatccaaataaaaaaaaagcaaaaaaaaacataaaatgaaaggggaggcaaataaaaaaccggtgagccgaaaagaggtacaccgagcagagtgaaggaatgagtgtttggtccaaaaccaaggaaatacaatgtataggataaagctgttgtaacacacagtcattttttcattccattgaaaacactaaacgtcttgttgaactgacacgtcggcaatggccgcagatcgtgctgggcatcagctcgttcctgatcgagccgatgtgccgtcggctgggcgcccgcgcggtgtgggtgatgagcagcgccgtcgtgtgcgtcgccatggcggccgtctccgtgctcagcgcgtggtcgctcggcgacttcggcggctcggtgcaggacgcagcgcgggcgccggcggaggagggcggcgtcagggcgtcggcgctggcgctcttcgtcttcctcggcctccccttcgccgtcctctgcagcgtcccgttcgccgtcacggcgcagctcgcggcgagccgcggcggcgggcagggcctctgcaccggcgtcctcaacatctccatcgtcgtgccgcagatggccatcgccctcggcgccgggccctgggacgagctgttcggggaggggaacatcccggcgttcgccatggcgtccgtgttcgccgccgcggccgccgccgccggcgtcgtcttgctgcccaaggtctccgtccgctccgtcagcatggccggcggccactgatcactactagtctatcaccctttttttttccgtggttttaaatctcccgctatagctgctgctatctccagttagagcgggtactatagcaggctataagccagctgt</dnaseqindica>
External Link(s)	NCBI Gene:Os10g0404500, RefSeq:Os10g0404500