Difference between revisions of "Os09g0451500"
(→Function) |
|||
| Line 3: | Line 3: | ||
==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
| − | + | ||
| + | Complementation analyses using PDIL1;1 knockout esp2 mutant indicated that the a and a' TRX domains of PDIL1;1 exhibited similar redox activities and that PDIL2;3 was unable to perform the PDIL1;1 functions. PDIL2;3 knockdown inhibited the accumulation of Cys-rich 10-kD prolamin (crP10) in the core of PB-I. Conversely, crP10 knockdown dispersed PDIL2;3 into the ER lumen. Glutathione S-transferase-PDIL2;3 formed a stable tetramer when it was expressed in Escherichia coli, and the recombinant PDIL2;3 tetramer facilitated α-globulin(C79F) mutant protein to form nonnative intermolecular disulfide bonds in vitro. These results indicate that PDIL2;3 and PDIL1;1 are not functionally redundant in sulfhydryl oxidations of structurally diverse storage proteins and play distinct roles in PB development. We discuss PDIL2;3-dependent and PDIL2;3-independent oxidation pathways that sustain disulfide bonds of crP10 in PB-I. | ||
===Expression=== | ===Expression=== | ||
Revision as of 03:56, 5 June 2014
Please input one-sentence summary here.
Contents
Annotated Information
Function
Complementation analyses using PDIL1;1 knockout esp2 mutant indicated that the a and a' TRX domains of PDIL1;1 exhibited similar redox activities and that PDIL2;3 was unable to perform the PDIL1;1 functions. PDIL2;3 knockdown inhibited the accumulation of Cys-rich 10-kD prolamin (crP10) in the core of PB-I. Conversely, crP10 knockdown dispersed PDIL2;3 into the ER lumen. Glutathione S-transferase-PDIL2;3 formed a stable tetramer when it was expressed in Escherichia coli, and the recombinant PDIL2;3 tetramer facilitated α-globulin(C79F) mutant protein to form nonnative intermolecular disulfide bonds in vitro. These results indicate that PDIL2;3 and PDIL1;1 are not functionally redundant in sulfhydryl oxidations of structurally diverse storage proteins and play distinct roles in PB development. We discuss PDIL2;3-dependent and PDIL2;3-independent oxidation pathways that sustain disulfide bonds of crP10 in PB-I.
Expression
Please input expression information here.
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this gene
Please input related labs here.
References
Please input cited references here.
Structured Information
| Gene Name |
Os09g0451500 |
|---|---|
| Description |
Thioredoxin domain 2 containing protein |
| Version |
NM_001069866.1 GI:115479474 GeneID:4347226 |
| Length |
4864 bp |
| Definition |
Oryza sativa Japonica Group Os09g0451500, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 9:17584752..17589615 |
| Sequence Coding Region |
17584851..17584976,17585080..17585238,17586351..17586464,17587063..17587244,17587504..17587603 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008402:17584752..17589615 source=RiceChromosome09 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008402:17584752..17589615 source=RiceChromosome09 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgcgccccgcggtcgccgccgccctcctcctcgtcgccgccgccgtcgcggcctcgccggtttccgccctctactccgccggctctcccgtcctccagttcaaccccaacaacttcaaatccaaggtgctgaactcgaatggggtggtgctggtggagttcttcgcgccgtggtgcgggcactgccagcagctgacgccgatttgggagaaggccgcgggcgtcctcaagggcgtcgccacggtcgccgcgctcgacgccgacgcgcacaaggagctcgcgcaggaatatggaataagaggatttccaactattaaggtgtttgttcctggaaagcctccagtagattatcaaggagcaagagatgtaaagccaattgttgaatttgctctttcacaggtcaaggctcttcttagagatcggttgaatggaaagacatcagcaggttcaggtggcaagaaatcaggtggttcaagtgagaaaactgaaccaagtgcgtcaatagaattaaattctcaaaacttcgataaacttgtcaccaaaagcaaggatctttggattgttgagttctttgcaccatggtgtgggcactgcaagaaattggcacctgaatggaaaaaggctgcaaagaacttgaagggccaagtgaagctaggtcatgtggattgtgatgctgaaaagtctttgatgagcaagtacaaggtagaaggctttcctactattttggtatttggtgccgataaggagagcccattcccttaccagggggctagagttgcctctgctatcgagtcctttgcattggagcagttggaagccaatgctgctccacctgaagtttctgagttgactggcccagacgccatggaagagaaatgtgcttctgctgccatttgctttgtatctttccttccagatatcttggattcaaaggccgaaggaagaaacaagtaccttgagctgctattatctgttgctgagaaatttaaaaagagtccatacagttttgtctggacagctgctgggaagcaagctgatcttgagaagcaagttggagttggtggttatggctatccagcgatggttgctctcaacgtgaaaaaaggcgcatatgctccactccgtagtgctttccagcttgatgaaataactgagtttgtgaaggaagcagggcgcggtgggaagggtaatcttcctttggacggtaccccaacgatagtccaatctgaaccatgggacggcaaagatggtgaggttattgaagaggacgaattctcccttgaggagctgatggccgacaactctccagtaaatgacgagttgtga</cdnaseq> |
| Protein Sequence |
<aaseq>MRPAVAAALLLVAAAVAASPVSALYSAGSPVLQFNPNNFKSKVL NSNGVVLVEFFAPWCGHCQQLTPIWEKAAGVLKGVATVAALDADAHKELAQEYGIRGF PTIKVFVPGKPPVDYQGARDVKPIVEFALSQVKALLRDRLNGKTSAGSGGKKSGGSSE KTEPSASIELNSQNFDKLVTKSKDLWIVEFFAPWCGHCKKLAPEWKKAAKNLKGQVKL GHVDCDAEKSLMSKYKVEGFPTILVFGADKESPFPYQGARVASAIESFALEQLEANAA PPEVSELTGPDAMEEKCASAAICFVSFLPDILDSKAEGRNKYLELLLSVAEKFKKSPY SFVWTAAGKQADLEKQVGVGGYGYPAMVALNVKKGAYAPLRSAFQLDEITEFVKEAGR GGKGNLPLDGTPTIVQSEPWDGKDGEVIEEDEFSLEELMADNSPVNDEL</aaseq> |
| Gene Sequence |
<dnaseqindica>100..225#329..487#1600..1713#2312..2493#2753..2852#3429..3605#3695..3837#4241..4390#4473..4647#cctcgcgagcgaacttaaaatcgcagtcgcaccccggcgccgatcgcaaggtccacctcctcccctctccactccttctagaaccttctcgaccccgccatgcgccccgcggtcgccgccgccctcctcctcgtcgccgccgccgtcgcggcctcgccggtttccgccctctactccgccggctctcccgtcctccagttcaaccccaacaacttcaaatccaaggtctctcgatcgactcccccctcacactcttcccttccgtctccggatcggcttcccttacttgggggctctcgctgagttcgaatttcggttgggggtgcaggtgctgaactcgaatggggtggtgctggtggagttcttcgcgccgtggtgcgggcactgccagcagctgacgccgatttgggagaaggccgcgggcgtcctcaagggcgtcgccacggtcgccgcgctcgacgccgacgcgcacaaggagctcgcgcaggtgagcaaacgtcctgtttggcatctcggttcattgggattggctgatctaccttttcctggtgtgatctgatctctgcatcagttgttgcgtcaacgtaatttgatcgagctaggacataactgcgtatatgcaatgtgcaaactcgactccataaaaaaaaaagttacacctgttatgggttttttgtgtagctctcagtatcagagagtacaatgccacatttgtttggtggaggaaatatgggaagatcaatgtttggtgtctacacgtactgatcaaaaaggggcgtcgtttttattttttaaaggagggcattaacatcggatatgagttatacaataccgaattaatgcatattaaaatgctgtagctgggattaatattgatggaatgtggtgcattgttgatttcatacagtaaatttatttattttttcactttttcagcatggtgattggatgctgttctcttttttttaaaaaaaataacctggattttgaattatattgaacgaattaagcattttaattggtgttaattagggttttatctgtttttcttttgatccgaaggtgagagcggccttggttagtttattgccaataaaagtgtatatgtgactttagatgtgggaaatgcatacttttatgaaggacgaaaccgtggaagccaatgggttgtgcaactttacaatatctgatgcaacattgtttattggtgaatcagaaaattaggttatgtatcccagattctctaactgaaaatttagtcactcatcaaatgaaataaaggcatctatcctgagagaaggcaagcaagagtgaaagatagttatgtacctttgccattgtacctgcagtgcggtgattttgagtctctgtcatatttagacatatatttttttttacagtaattttacagtatcatgcttattgaccaagaggcattttttagctacgttatatctttgttttggtgagtattagtcttctgtctaggctaaatcaataaaaactgcacattagaacgtcttccaccattgccatttgtaaatattttgactggatgctatcaagtttttcctgatgcatgatgcctttgttctgcaggaatatggaataagaggatttccaactattaaggtgtttgttcctggaaagcctccagtagattatcaaggagcaagagatgtaaagccaattgttgaatttgctctttcacaggtttgttgcagcttgccctatttgatatatataaaaatgatatgttgtttttacttttattgctcctgttctatctgccactccactagtgtatacaactacaaatccttttctgaagatgttaagtgcttcctggattttcacagaccaaacttattcagattgcaattgtcagctgaatgttgacataaataacttttatcttaacatgaagtattactaggggggaaaaacaacagatatcatatatcaactgatactaatgtcctaggtagctaattgattttgctatatatctccctcaaatgctaaacctttcaaacttatcaactttttatatgggatactggatatgaggtgatgcagtgatgcttctcagttcttaccatggtggagctttatgtacctgctcttgcacagctggtgcttctggtagcacccatgattttcgttaaaaaatacactgtctgtcgctgctgtgttacagtgggttgccattttaccttttttttttgggtatttctctgtaaaaaatttcagccttgtgacacacgatatgagtttccacttttaaatacaatgaatctgtatcctacaggtcaaggctcttcttagagatcggttgaatggaaagacatcagcaggttcaggtggcaagaaatcaggtggttcaagtgagaaaactgaaccaagtgcgtcaatagaattaaattctcaaaacttcgataaacttgtcaccaaaagcaaggatctttggattgttgagttctttgcaccatggtatgtggcttaatgcatttatggttttcatgttgactgatttattctttactgttgtttctgaacttaactctgaatccaaaagtagagaaggaaagtgaagaaaaacacaaaatttcatacaatttagtcacatgttagtattgtagtgtgtacattttagatcattgcccttaagattatgttgttcaagataataagtaaatcctgttaactgaagacattatttgactcattaactgtactggtcttttgtaaggtgtgggcactgcaagaaattggcacctgaatggaaaaaggctgcaaagaacttgaagggccaagtgaagctaggtcatgtggattgtgatgctgaaaaggttcttgccctttttctatttggtcataagtttttgttgtatgtgcatttcttgtttgcaaataatttatacaaaattataaactgaaatttgacaatgcattggatatttcatcaattcaaaggactttgataaaggtattgtaaaataaaattaaactgagggttagcactctagtttatagggacacaatcaggatatgcataggaaagtacaaaatgctgtatcaaggaaagcaatggcctaactttatcctaaggcatttgttgggatcttattttgtgctgttttaggttgtctctttaacagtttaacatgatgtactatgattgaagttttatgtttttttttctagaatactaattcaagtgtttggtcagttgataaaagttaccgagtgagtttaaatgaacttaggcagaaaattcaggcagtgtatcagtatatttataaaacacagttaatgtttgacctttttaaagcattcatgtgcgttctgtacaacatttgtcaaactatacaacctagttttcaagtaccacttatgtaacataacgtgtgacttgaattttgcagtctttgatgagcaagtacaaggtagaaggctttcctactattttggtatttggtgccgataaggagagcccattcccttaccagggggctagagttgcctctgctatcgagtcctttgcattggagcagttggaagccaatgctgctccacctgaagtttctgagttgactggcccagtaagttttcgtgatattaacaaagttatccttttgtggagacaatggtctgggattgatgttttgacgaggtggtttggcttctgtaggacgccatggaagagaaatgtgcttctgctgccatttgctttgtatctttccttccagatatcttggattcaaaggccgaaggaagaaacaagtaccttgagctgctattatctgttgctgagaaatttaaaaagagtccatacaggcaagattcaactaaacttgttgtggactttataccattctacctatcattccttgtcttgttatgtcttttgagataaaaaaatccacagttaaattgatacattaatttccttttgctattgaatattgattgacaaaaagattgatagtaaccttcatatatttgaaacaccttaaaagaattaaatgacttagttcgataatgttatatgccaaggcgtgtatactataagctctaaacataatggagattttatatattatatatgcaaatctgccatgtgactgagttctatgttgagaagtttcctcagacaaatccattcacttagttaatgccttgaaatctcttccgtttcttgagaaagccattctgagctagaaaaagcattgtcttgcagttttgtctggacagctgctgggaagcaagctgatcttgagaagcaagttggagttggtggttatggctatccagcgatggttgctctcaacgtgaaaaaaggcgcatatgctccactccgtagtgctttccagcttgatgaaataacgtaagttccttgtgcatagaaaattaattgctgcttaaggttcatttgccagatgtttgataacaaaaccggtctcctgcagtgagtttgtgaaggaagcagggcgcggtgggaagggtaatcttcctttggacggtaccccaacgatagtccaatctgaaccatgggacggcaaagatggtgaggttattgaagaggacgaattctcccttgaggagctgatggccgacaactctccagtaaatgacgagttgtgattctgataattccggaaatgatgttagagttgattagccagactgtaaatctgagtaacactaatgcttcactcgtgtcaactgtacaatttgggcgggacaattttcagaatgctcctttggtgttattacagtacattttagttgaagaaaaacatcgtttgtggtcttttaggagggatgcttttctctgggaaataaaactacttcgtaatct</dnaseqindica> |
| External Link(s) |
