Difference between revisions of "Os09g0451500"
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===Expression=== | ===Expression=== | ||
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| + | Complementation analyses using PDIL1;1 knockout esp2 mutant indicated that the a and a' TRX domains of PDIL1;1 exhibited similar redox activities and that PDIL2;3 was unable to perform the PDIL1;1 functions. PDIL2;3 knockdown inhibited the accumulation of Cys-rich 10-kD prolamin (crP10) in the core of PB-I. Conversely, crP10 knockdown dispersed PDIL2;3 into the ER lumen. Glutathione S-transferase-PDIL2;3 formed a stable tetramer when it was expressed in Escherichia coli, and the recombinant PDIL2;3 tetramer facilitated α-globulin(C79F) mutant protein to form nonnative intermolecular disulfide bonds in vitro. | ||
===Evolution=== | ===Evolution=== | ||
Revision as of 03:59, 5 June 2014
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Contents
Annotated Information
Function
PDIL2;3 and PDIL1;1 are not functionally redundant in sulfhydryl oxidations of structurally diverse storage proteins and play distinct roles in PB development. PDIL2;3-dependent and PDIL2;3-independent oxidation pathways that sustain disulfide bonds of crP10 in PB-I.
Expression
Complementation analyses using PDIL1;1 knockout esp2 mutant indicated that the a and a' TRX domains of PDIL1;1 exhibited similar redox activities and that PDIL2;3 was unable to perform the PDIL1;1 functions. PDIL2;3 knockdown inhibited the accumulation of Cys-rich 10-kD prolamin (crP10) in the core of PB-I. Conversely, crP10 knockdown dispersed PDIL2;3 into the ER lumen. Glutathione S-transferase-PDIL2;3 formed a stable tetramer when it was expressed in Escherichia coli, and the recombinant PDIL2;3 tetramer facilitated α-globulin(C79F) mutant protein to form nonnative intermolecular disulfide bonds in vitro.
Evolution
Protein disulfide isomerase (PDI) family oxidoreductase PDIL2;3, an ortholog of human P5, contains a conserved structural disulfide in the redox-inactive thioredoxin-like (TRX) domain and was efficiently targeted to the surface of PB-I in a redox active site-dependent manner, whereas PDIL1;1, an ortholog of human PDI, was localized in the ER lumen.
Labs working on this gene
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References
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Structured Information
| Gene Name |
Os09g0451500 |
|---|---|
| Description |
Thioredoxin domain 2 containing protein |
| Version |
NM_001069866.1 GI:115479474 GeneID:4347226 |
| Length |
4864 bp |
| Definition |
Oryza sativa Japonica Group Os09g0451500, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 9:17584752..17589615 |
| Sequence Coding Region |
17584851..17584976,17585080..17585238,17586351..17586464,17587063..17587244,17587504..17587603 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008402:17584752..17589615 source=RiceChromosome09 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008402:17584752..17589615 source=RiceChromosome09 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgcgccccgcggtcgccgccgccctcctcctcgtcgccgccgccgtcgcggcctcgccggtttccgccctctactccgccggctctcccgtcctccagttcaaccccaacaacttcaaatccaaggtgctgaactcgaatggggtggtgctggtggagttcttcgcgccgtggtgcgggcactgccagcagctgacgccgatttgggagaaggccgcgggcgtcctcaagggcgtcgccacggtcgccgcgctcgacgccgacgcgcacaaggagctcgcgcaggaatatggaataagaggatttccaactattaaggtgtttgttcctggaaagcctccagtagattatcaaggagcaagagatgtaaagccaattgttgaatttgctctttcacaggtcaaggctcttcttagagatcggttgaatggaaagacatcagcaggttcaggtggcaagaaatcaggtggttcaagtgagaaaactgaaccaagtgcgtcaatagaattaaattctcaaaacttcgataaacttgtcaccaaaagcaaggatctttggattgttgagttctttgcaccatggtgtgggcactgcaagaaattggcacctgaatggaaaaaggctgcaaagaacttgaagggccaagtgaagctaggtcatgtggattgtgatgctgaaaagtctttgatgagcaagtacaaggtagaaggctttcctactattttggtatttggtgccgataaggagagcccattcccttaccagggggctagagttgcctctgctatcgagtcctttgcattggagcagttggaagccaatgctgctccacctgaagtttctgagttgactggcccagacgccatggaagagaaatgtgcttctgctgccatttgctttgtatctttccttccagatatcttggattcaaaggccgaaggaagaaacaagtaccttgagctgctattatctgttgctgagaaatttaaaaagagtccatacagttttgtctggacagctgctgggaagcaagctgatcttgagaagcaagttggagttggtggttatggctatccagcgatggttgctctcaacgtgaaaaaaggcgcatatgctccactccgtagtgctttccagcttgatgaaataactgagtttgtgaaggaagcagggcgcggtgggaagggtaatcttcctttggacggtaccccaacgatagtccaatctgaaccatgggacggcaaagatggtgaggttattgaagaggacgaattctcccttgaggagctgatggccgacaactctccagtaaatgacgagttgtga</cdnaseq> |
| Protein Sequence |
<aaseq>MRPAVAAALLLVAAAVAASPVSALYSAGSPVLQFNPNNFKSKVL NSNGVVLVEFFAPWCGHCQQLTPIWEKAAGVLKGVATVAALDADAHKELAQEYGIRGF PTIKVFVPGKPPVDYQGARDVKPIVEFALSQVKALLRDRLNGKTSAGSGGKKSGGSSE KTEPSASIELNSQNFDKLVTKSKDLWIVEFFAPWCGHCKKLAPEWKKAAKNLKGQVKL GHVDCDAEKSLMSKYKVEGFPTILVFGADKESPFPYQGARVASAIESFALEQLEANAA PPEVSELTGPDAMEEKCASAAICFVSFLPDILDSKAEGRNKYLELLLSVAEKFKKSPY SFVWTAAGKQADLEKQVGVGGYGYPAMVALNVKKGAYAPLRSAFQLDEITEFVKEAGR GGKGNLPLDGTPTIVQSEPWDGKDGEVIEEDEFSLEELMADNSPVNDEL</aaseq> |
| Gene Sequence |
<dnaseqindica>100..225#329..487#1600..1713#2312..2493#2753..2852#3429..3605#3695..3837#4241..4390#4473..4647#cctcgcgagcgaacttaaaatcgcagtcgcaccccggcgccgatcgcaaggtccacctcctcccctctccactccttctagaaccttctcgaccccgccatgcgccccgcggtcgccgccgccctcctcctcgtcgccgccgccgtcgcggcctcgccggtttccgccctctactccgccggctctcccgtcctccagttcaaccccaacaacttcaaatccaaggtctctcgatcgactcccccctcacactcttcccttccgtctccggatcggcttcccttacttgggggctctcgctgagttcgaatttcggttgggggtgcaggtgctgaactcgaatggggtggtgctggtggagttcttcgcgccgtggtgcgggcactgccagcagctgacgccgatttgggagaaggccgcgggcgtcctcaagggcgtcgccacggtcgccgcgctcgacgccgacgcgcacaaggagctcgcgcaggtgagcaaacgtcctgtttggcatctcggttcattgggattggctgatctaccttttcctggtgtgatctgatctctgcatcagttgttgcgtcaacgtaatttgatcgagctaggacataactgcgtatatgcaatgtgcaaactcgactccataaaaaaaaaagttacacctgttatgggttttttgtgtagctctcagtatcagagagtacaatgccacatttgtttggtggaggaaatatgggaagatcaatgtttggtgtctacacgtactgatcaaaaaggggcgtcgtttttattttttaaaggagggcattaacatcggatatgagttatacaataccgaattaatgcatattaaaatgctgtagctgggattaatattgatggaatgtggtgcattgttgatttcatacagtaaatttatttattttttcactttttcagcatggtgattggatgctgttctcttttttttaaaaaaaataacctggattttgaattatattgaacgaattaagcattttaattggtgttaattagggttttatctgtttttcttttgatccgaaggtgagagcggccttggttagtttattgccaataaaagtgtatatgtgactttagatgtgggaaatgcatacttttatgaaggacgaaaccgtggaagccaatgggttgtgcaactttacaatatctgatgcaacattgtttattggtgaatcagaaaattaggttatgtatcccagattctctaactgaaaatttagtcactcatcaaatgaaataaaggcatctatcctgagagaaggcaagcaagagtgaaagatagttatgtacctttgccattgtacctgcagtgcggtgattttgagtctctgtcatatttagacatatatttttttttacagtaattttacagtatcatgcttattgaccaagaggcattttttagctacgttatatctttgttttggtgagtattagtcttctgtctaggctaaatcaataaaaactgcacattagaacgtcttccaccattgccatttgtaaatattttgactggatgctatcaagtttttcctgatgcatgatgcctttgttctgcaggaatatggaataagaggatttccaactattaaggtgtttgttcctggaaagcctccagtagattatcaaggagcaagagatgtaaagccaattgttgaatttgctctttcacaggtttgttgcagcttgccctatttgatatatataaaaatgatatgttgtttttacttttattgctcctgttctatctgccactccactagtgtatacaactacaaatccttttctgaagatgttaagtgcttcctggattttcacagaccaaacttattcagattgcaattgtcagctgaatgttgacataaataacttttatcttaacatgaagtattactaggggggaaaaacaacagatatcatatatcaactgatactaatgtcctaggtagctaattgattttgctatatatctccctcaaatgctaaacctttcaaacttatcaactttttatatgggatactggatatgaggtgatgcagtgatgcttctcagttcttaccatggtggagctttatgtacctgctcttgcacagctggtgcttctggtagcacccatgattttcgttaaaaaatacactgtctgtcgctgctgtgttacagtgggttgccattttaccttttttttttgggtatttctctgtaaaaaatttcagccttgtgacacacgatatgagtttccacttttaaatacaatgaatctgtatcctacaggtcaaggctcttcttagagatcggttgaatggaaagacatcagcaggttcaggtggcaagaaatcaggtggttcaagtgagaaaactgaaccaagtgcgtcaatagaattaaattctcaaaacttcgataaacttgtcaccaaaagcaaggatctttggattgttgagttctttgcaccatggtatgtggcttaatgcatttatggttttcatgttgactgatttattctttactgttgtttctgaacttaactctgaatccaaaagtagagaaggaaagtgaagaaaaacacaaaatttcatacaatttagtcacatgttagtattgtagtgtgtacattttagatcattgcccttaagattatgttgttcaagataataagtaaatcctgttaactgaagacattatttgactcattaactgtactggtcttttgtaaggtgtgggcactgcaagaaattggcacctgaatggaaaaaggctgcaaagaacttgaagggccaagtgaagctaggtcatgtggattgtgatgctgaaaaggttcttgccctttttctatttggtcataagtttttgttgtatgtgcatttcttgtttgcaaataatttatacaaaattataaactgaaatttgacaatgcattggatatttcatcaattcaaaggactttgataaaggtattgtaaaataaaattaaactgagggttagcactctagtttatagggacacaatcaggatatgcataggaaagtacaaaatgctgtatcaaggaaagcaatggcctaactttatcctaaggcatttgttgggatcttattttgtgctgttttaggttgtctctttaacagtttaacatgatgtactatgattgaagttttatgtttttttttctagaatactaattcaagtgtttggtcagttgataaaagttaccgagtgagtttaaatgaacttaggcagaaaattcaggcagtgtatcagtatatttataaaacacagttaatgtttgacctttttaaagcattcatgtgcgttctgtacaacatttgtcaaactatacaacctagttttcaagtaccacttatgtaacataacgtgtgacttgaattttgcagtctttgatgagcaagtacaaggtagaaggctttcctactattttggtatttggtgccgataaggagagcccattcccttaccagggggctagagttgcctctgctatcgagtcctttgcattggagcagttggaagccaatgctgctccacctgaagtttctgagttgactggcccagtaagttttcgtgatattaacaaagttatccttttgtggagacaatggtctgggattgatgttttgacgaggtggtttggcttctgtaggacgccatggaagagaaatgtgcttctgctgccatttgctttgtatctttccttccagatatcttggattcaaaggccgaaggaagaaacaagtaccttgagctgctattatctgttgctgagaaatttaaaaagagtccatacaggcaagattcaactaaacttgttgtggactttataccattctacctatcattccttgtcttgttatgtcttttgagataaaaaaatccacagttaaattgatacattaatttccttttgctattgaatattgattgacaaaaagattgatagtaaccttcatatatttgaaacaccttaaaagaattaaatgacttagttcgataatgttatatgccaaggcgtgtatactataagctctaaacataatggagattttatatattatatatgcaaatctgccatgtgactgagttctatgttgagaagtttcctcagacaaatccattcacttagttaatgccttgaaatctcttccgtttcttgagaaagccattctgagctagaaaaagcattgtcttgcagttttgtctggacagctgctgggaagcaagctgatcttgagaagcaagttggagttggtggttatggctatccagcgatggttgctctcaacgtgaaaaaaggcgcatatgctccactccgtagtgctttccagcttgatgaaataacgtaagttccttgtgcatagaaaattaattgctgcttaaggttcatttgccagatgtttgataacaaaaccggtctcctgcagtgagtttgtgaaggaagcagggcgcggtgggaagggtaatcttcctttggacggtaccccaacgatagtccaatctgaaccatgggacggcaaagatggtgaggttattgaagaggacgaattctcccttgaggagctgatggccgacaactctccagtaaatgacgagttgtgattctgataattccggaaatgatgttagagttgattagccagactgtaaatctgagtaacactaatgcttcactcgtgtcaactgtacaatttgggcgggacaattttcagaatgctcctttggtgttattacagtacattttagttgaagaaaaacatcgtttgtggtcttttaggagggatgcttttctctgggaaataaaactacttcgtaatct</dnaseqindica> |
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