Difference between revisions of "Qsw5"
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The biological function of gene Qsw5 is that it through increasing the number of cells in rice flowers lemma,then increaseing the capacity of rice husk, and ultimately increase the grain width.Pets the gene loci of Kasalath qSW5, the grain width lines into smaller, field production decreased by 10%.But through the gene RNAi regulate ORF1's expression down (ORF1 is one sets of Kasalath qSW5) ,then can make larger grain width and increase output.Therefore, loss of function qSW5 sites have value in breeding. | The biological function of gene Qsw5 is that it through increasing the number of cells in rice flowers lemma,then increaseing the capacity of rice husk, and ultimately increase the grain width.Pets the gene loci of Kasalath qSW5, the grain width lines into smaller, field production decreased by 10%.But through the gene RNAi regulate ORF1's expression down (ORF1 is one sets of Kasalath qSW5) ,then can make larger grain width and increase output.Therefore, loss of function qSW5 sites have value in breeding. | ||
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Expression | Expression | ||
| − | + | As Darwin (1857) noted, crop domestication resembles a rapid evolutionary process that results from artificial selection but that otherwise has all the characteristics of evolution by means of natural selection. With increasing availability of crop genome information, the accumulation of knowledge about domestication-related genes, and the identification of functional nucleotide polymorphisms (FNPs), the crop domestication process is being increasingly elucidated (Doebley et al. 2006). In maize, for example, extensive genome analysis to find genes with reduced natural variation among cultivars, landraces and their wild relatives has suggested that thousands of genes might have been subjected to selection during domestication (Gaut et al. 2000, Matsuoka et al. 2002, Yamasaki et al. 2005). In wheat, archeological analysis of plant remains has revealed that the domestication process took a few thousand years (Tanno and Willcox 2006, Dubcovsky and Dvorak 2007). In barley, natural variation among landraces from Europe to Asia has been examined to reveal how barley cultivation propagated throughout these regions, with some genome changes detected and two distinct domestication processes proposed for European and Asian barley (Morrell and Clegg 2007, Pourkheirandish and Komatsuda 2007, Saisho and Purugganan 2007). In addition, some domestication-related genes have been cloned, and key natural variations in these genes (i.e. FNPs) have been examined to elucidate the domestication process in several crops, including maize, wheat and barley (Wang et al. 2005, Doebley et al. 2006, Simons et al. 2006, Komatsuda et al. 2007), even though the domestication process itself remains largely unknown. | |
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Evolution | Evolution | ||
We can express the OsSUT gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source. | We can express the OsSUT gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source. | ||
Revision as of 04:26, 6 June 2014
Function
The biological function of gene Qsw5 is that it through increasing the number of cells in rice flowers lemma,then increaseing the capacity of rice husk, and ultimately increase the grain width.Pets the gene loci of Kasalath qSW5, the grain width lines into smaller, field production decreased by 10%.But through the gene RNAi regulate ORF1's expression down (ORF1 is one sets of Kasalath qSW5) ,then can make larger grain width and increase output.Therefore, loss of function qSW5 sites have value in breeding.
Expression
As Darwin (1857) noted, crop domestication resembles a rapid evolutionary process that results from artificial selection but that otherwise has all the characteristics of evolution by means of natural selection. With increasing availability of crop genome information, the accumulation of knowledge about domestication-related genes, and the identification of functional nucleotide polymorphisms (FNPs), the crop domestication process is being increasingly elucidated (Doebley et al. 2006). In maize, for example, extensive genome analysis to find genes with reduced natural variation among cultivars, landraces and their wild relatives has suggested that thousands of genes might have been subjected to selection during domestication (Gaut et al. 2000, Matsuoka et al. 2002, Yamasaki et al. 2005). In wheat, archeological analysis of plant remains has revealed that the domestication process took a few thousand years (Tanno and Willcox 2006, Dubcovsky and Dvorak 2007). In barley, natural variation among landraces from Europe to Asia has been examined to reveal how barley cultivation propagated throughout these regions, with some genome changes detected and two distinct domestication processes proposed for European and Asian barley (Morrell and Clegg 2007, Pourkheirandish and Komatsuda 2007, Saisho and Purugganan 2007). In addition, some domestication-related genes have been cloned, and key natural variations in these genes (i.e. FNPs) have been examined to elucidate the domestication process in several crops, including maize, wheat and barley (Wang et al. 2005, Doebley et al. 2006, Simons et al. 2006, Komatsuda et al. 2007), even though the domestication process itself remains largely unknown.
Evolution
We can express the OsSUT gene in yeast to test whether it's functional. Choose the yeast strain which is unable to hydrolyse exogenous sucrose but if transformed with a functional SUT, can import sucrose and hydrolyse it internally , allowing it to grow on media containing sucrose as the sole carbon source.
" Test function in yeast(from reference [2]).. "
There is also a novel fluorescent assay for sucrose transporter activity based on the ability of type I SUTs to transport the highly fluorescent molecule esculin (6,7-dihydroxycoumarin β-D-glucoside). Using fluorescence microscopy, we can do the research conveniently.
" Esculin uptake by yeast cells expressing StSUT1 was detected using FACS.(from reference [5]).. "Labs working on this gene
Univ Minnesota, Dept Plant Biol, Biol Sci Ctr 250, 1445 Gortner Ave, St Paul, MN 55108 USA. CSIRO Plant Ind, Canberra, ACT 2601, Australia. Chinese Acad Sci, Inst Genet & Dev Biol, Natl Key Lab Plant Genom, Beijing 100101, Peoples R China Natl Agr Res Ctr, Dept Rice Res, Niigata 9430193, Japan National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences,Peoples R China Department of Rice Research, National Agricultural Research Center, Joetsu, Niigata, 943-0193 JapanReferences
↑ Lu J M-Y. and Bush D R.(1998) His-65 in the proton-sucrose symporter is an essential amino acid whose modification with site-directed mutagenesis increases transport activity. Proc Natl Acad 95: 9025–9030. ↑ 2.0 2.1 2.2 Aoki N, Hirose T, Scofield G N, et al.(2003）The Sucrose Transporter Gene Family in Rice. Plant and Cell Physiology 44:223-232. ↑ Furbank R T, Scofield G N, Hirose T, et al. (2001) Cellular localisation and function of a sucrose transporter OsSUT1 in developing rice grains. Aust. J. Plant Physiol 28: 1187–1196. ↑ Hirose T, Imaizumi N, Scofield G N, et al. (1997) cDNA cloning and tissue-specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol 38: 1389–1396. ↑ Gora P J, Reinders A, Ward J M, et al.(2012)A novel fluorescent assay for sucrose transporters. Plant Methods 8:13.
