Difference between revisions of "Os02g0148100"
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| − | + | OsMAPK33 (Os02g0148100) is a Mitogen-activated protein kinases (MAPK) cDNA clone in rice, which is mainly induced by drought stress | |
==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
| − | + | Under dehydration conditions, the suppressed lines showed lower osmotic potential compared with that of wild-type plants, suggesting a role ofOsMAPK33 in osmotic homeostasis. Nonetheless, the suppressed lines did not display any significant difference in drought tolerance compared with their wild-type plants. With increased salinity, there was still no difference in salt tolerance between OsMAPK33-suppressed lines and their wild-type plants. However, the overexpressing lines showed greater reduction in biomass accumulation and higher sodium uptake into cells, resulting in a lower K+/Na+ ratio inside the cell than that in the wild-type plants andOsMAPK33-suppressed lines. These results suggest that OsMAPK33could play a negative role in salt tolerance through unfavourable ion homeostasis. Gene expression profiling ofOsMAPK33transgenic lines through rice DNA chip analysis showed that OsMAPK33altered expression of genes involved in ion transport.<ref name="ref1" /> | |
===Expression=== | ===Expression=== | ||
| − | + | OsMAPK33 is found to exhibit organ-specific expression with relatively higher expression in leaves as compared with roots or stems, and to exist as a single copy in the rice genome.<ref name="ref1" /> | |
===Evolution=== | ===Evolution=== | ||
| − | + | OsMAPK33 showed approximately 47–93% identity at the amino acid level with other plant MAPKs.<ref name="ref1" /> | |
| − | + | ==Labs working on this gene== | |
| + | 1. Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration, Suwon 441–857, Republic of Korea | ||
| + | |||
| + | 2. Department of Biomedical Science, Sun Moon University, Asan 336–708, Republic of Korea | ||
| + | |||
| + | 3. Soybean Genomics and Improvement Lab, USDA-ARS, Beltsville, MD 20705, USA | ||
| − | + | 4. Department of Biological Sciences, Seoul National University, Seoul 151–742, Republic of Korea | |
| − | |||
==References== | ==References== | ||
| − | + | <references> | |
| + | <ref name="ref1"> | ||
| + | Lee S-K, Kim B-G, Kwon T-R, Jeong M-J, Park S-R, Lee J-W, Byun M-O, Kwon H-B, Matthews BF, Hong C-B and Park S-C 2011 Overexpression of the mitogen-activated protein kinase geneOsMAPK33enhances sensitivity to salt stress in rice (Oryza sativaL.).J. Biosci.36 139–151 | ||
| + | </ref> | ||
| + | </references> | ||
==Structured Information== | ==Structured Information== | ||
Revision as of 08:18, 6 June 2014
OsMAPK33 (Os02g0148100) is a Mitogen-activated protein kinases (MAPK) cDNA clone in rice, which is mainly induced by drought stress
Contents
Annotated Information
Function
Under dehydration conditions, the suppressed lines showed lower osmotic potential compared with that of wild-type plants, suggesting a role ofOsMAPK33 in osmotic homeostasis. Nonetheless, the suppressed lines did not display any significant difference in drought tolerance compared with their wild-type plants. With increased salinity, there was still no difference in salt tolerance between OsMAPK33-suppressed lines and their wild-type plants. However, the overexpressing lines showed greater reduction in biomass accumulation and higher sodium uptake into cells, resulting in a lower K+/Na+ ratio inside the cell than that in the wild-type plants andOsMAPK33-suppressed lines. These results suggest that OsMAPK33could play a negative role in salt tolerance through unfavourable ion homeostasis. Gene expression profiling ofOsMAPK33transgenic lines through rice DNA chip analysis showed that OsMAPK33altered expression of genes involved in ion transport.[1]
Expression
OsMAPK33 is found to exhibit organ-specific expression with relatively higher expression in leaves as compared with roots or stems, and to exist as a single copy in the rice genome.[1]
Evolution
OsMAPK33 showed approximately 47–93% identity at the amino acid level with other plant MAPKs.[1]
Labs working on this gene
1. Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration, Suwon 441–857, Republic of Korea
2. Department of Biomedical Science, Sun Moon University, Asan 336–708, Republic of Korea
3. Soybean Genomics and Improvement Lab, USDA-ARS, Beltsville, MD 20705, USA
4. Department of Biological Sciences, Seoul National University, Seoul 151–742, Republic of Korea
References
Structured Information
| Gene Name |
Os02g0148100 |
|---|---|
| Description |
MAP kinase MAPK2 (MAP kinase 3) |
| Version |
NM_001052424.1 GI:115444218 GeneID:4328297 |
| Length |
3305 bp |
| Definition |
Oryza sativa Japonica Group Os02g0148100, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 2:2643054..2646358 |
| Sequence Coding Region |
2643310..2643999,2645043..2645465 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008395:2643054..2646358 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008395:2643054..2646358 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggcgatcatggtggatcctccaaatgggatgggtaaccaagggaagtattactactcgatgtggcaaactttgtttgagatagacactaagtatgtgccaatcaagcccatcgggcgaggagcttatgggattgtttgttcatccataaatcgtgagaccaacgagaaagtagcaataaagaagatacacaatgtttttgacaaccgtgttgatgcactaaggactttgcgggagctgaaacttctccggcatctccgccatgagaatgttattgctttgaaggatataatgatgccagtacacaggaggagttttaaagatgtgtacttagtttatgaactcatggatactgacctgcatcagataatcaagtcacctcagggtctttccaatgatcactgccaatattttctttttcagttgcttcgaggactgaaatatctccattcagcagagatactccacagagacctgaaacctggaaatctactggttaatgcaaactgtgatctcaagatatgtgattttggtcttgcacgcacaaacagtagtaaaggtcagtttatgactgagtatgttgtcacccgctggtatagagctcctgagttgctcctttgctgtgacaactatggcacttccattgatgtttggtctgttggttgcatcttcgctgagctacttggtcggaaacctattttcccaggaactgagtgcctaaatcagctcaagctcattgtcaatgttcttggcaccatgagcgagtctgacttggagttcattgacaacccaaaagctcgcagatatatcaaatccctcccctacactcccggtgtgcccctcgcgagtatgtatccgcatgcacaccctcttgccattgatcttttacagaagatgctcatatttgatcctaccaaaagaatcagtgtcaccgaggctcttgagcacccttacatgtcccctctgtatgatccaagtgcaaatcctcccgcgcaagtgcctatcgatctggacatagacgagaacatcagtgcagatatgatcagggaaatgatgtggcacgagatgctccactaccaccctgaagttgttgcagcaatgagtgcccgatga</cdnaseq> |
| Protein Sequence |
<aaseq>MAIMVDPPNGMGNQGKYYYSMWQTLFEIDTKYVPIKPIGRGAYG IVCSSINRETNEKVAIKKIHNVFDNRVDALRTLRELKLLRHLRHENVIALKDIMMPVH RRSFKDVYLVYELMDTDLHQIIKSPQGLSNDHCQYFLFQLLRGLKYLHSAEILHRDLK PGNLLVNANCDLKICDFGLARTNSSKGQFMTEYVVTRWYRAPELLLCCDNYGTSIDVW SVGCIFAELLGRKPIFPGTECLNQLKLIVNVLGTMSESDLEFIDNPKARRYIKSLPYT PGVPLASMYPHAHPLAIDLLQKMLIFDPTKRISVTEALEHPYMSPLYDPSANPPAQVP IDLDIDENISADMIREMMWHEMLHYHPEVVAAMSAR</aaseq> |
| Gene Sequence |
<dnaseqindica>2360..3049#894..1316#atccacacctcacgggaagctgtcccttctcctcttctcctcctcggctgcggccacggcgacgacgacgacgacgaattcgagcgaggcgatcggcgatggaggcggtggcgatctgatccgctcttcccgtctcggcctcgcgctcctctccctccctcgcggtgtctcggggtgaggggaatcttttgtttttgttttttttgaccgtactgttctggctgggtgtttcttggtcgtggtccaagatcggcgggtggtttgtttgtccgggctccatggatcgatctctctctgttgatctgtgtagctggagaagctcagctccgtgggaggattacgcgcgcgtgattggttgggctgtgtgtaaatttggttgccggccatttttgtgctccggtggtgaatttgggtggtgaggctgtctgcacaatccgtggcgatgagctgggaaatgtgatgggggtgtgctcgtatcgggatgatggtgggttgtttggttgacccggatgagtgatgatgattcacaatcttgcatccatgaaccgtgggatgggtgtccatgtctttctccgggattgggaattggtaccaccaccatggcggttctaaaggcgggaggagataaatgcaggcggcaatgcatgcttggtgatggagcatctgtatgagtgtgcggggatcgatgctccatgttcattcagaaccatagccgagcaactgaaagctcatcttgaagactgggactgcgtttaatttatagctcatctttgattacaaagttgtttgatcttctgcattcatagatagtttggcagttgtgtaagctcaaaaagccgttgttatttcctaaggtagtactaacttattttcttttgtttccttagtaggaaatggcgatcatggtggatcctccaaatgggatgggtaaccaagggaagtattactactcgatgtggcaaactttgtttgagatagacactaagtatgtgccaatcaagcccatcgggcgaggagcttatgggattgtttgttcatccataaatcgtgagaccaacgagaaagtagcaataaagaagatacacaatgtttttgacaaccgtgttgatgcactaaggactttgcgggagctgaaacttctccggcatctccgccatgagaatgttattgctttgaaggatataatgatgccagtacacaggaggagttttaaagatgtgtacttagtttatgaactcatggatactgacctgcatcagataatcaagtcacctcagggtctttccaatgatcactgccaatattttctttttcaggtaatacctggacctctcttttctattctattctacatttacttactcaagagtaattgtgtgtttttaatggcccttgatgattgcttagaatttgtgacagtgcatcttatatcggcaagcctcatccagtcttataagatggttcattcccaaatgcacgaggcacaatcttaacttgaatggttcttctgtcagttaaaacttcaaagtcaaactgattttttaaacatgtaaaattgtgaatgttttgcttcagcaatgatttgtgagcatgtcaatgacaatacttttttgagtaatgtattcctaatttgaaataaccaatgtaacttgtagtatcattcagtttgattccagttgatttgatgtttttgcattagtagtatttgaaaaaaactgaaaaaatggcctactatatttattccaacccagattccaacagtttagactgataataaatagatggaaggcaaaactatatccctgtagtctgcagctttgagacaaactaaactacacactttgtgcattaagtattgccatcatgattcctctttttatacattttattttaatttgtagagttgaacatttattttcatgtgtcacaggcacaacatccatgcattcccttgttgcctcatcaagtacataaaaaagcagctattctaatgtccgttcaacctaaaactggaactggaaccatctagccctttttagatttccatctattctaacttattttattaatttcgttaaaccacatttccattaagaatttcatatacagttcataatctaaaacctagatcttctttcaaatagttctgcatagaggcttctgcatgaaaccacacaagtagaccctcttgtttacaagacataaatatttttctgatgaattgtctgttcatcctacccttcagattcatttattctgttgtcttgagttcattttggattgtctaccatgtatttcttattttttgcaatccgtattttccagatgatttaagagaatgttgttttcttgattgtagttgcttcgaggactgaaatatctccattcagcagagatactccacagagacctgaaacctggaaatctactggttaatgcaaactgtgatctcaagatatgtgattttggtcttgcacgcacaaacagtagtaaaggtcagtttatgactgagtatgttgtcacccgctggtatagagctcctgagttgctcctttgctgtgacaactatggcacttccattgatgtttggtctgttggttgcatcttcgctgagctacttggtcggaaacctattttcccaggaactgagtgcctaaatcagctcaagctcattgtcaatgttcttggcaccatgagcgagtctgacttggagttcattgacaacccaaaagctcgcagatatatcaaatccctcccctacactcccggtgtgcccctcgcgagtatgtatccgcatgcacaccctcttgccattgatcttttacagaagatgctcatatttgatcctaccaaaagaatcagtgtcaccgaggctcttgagcacccttacatgtcccctctgtatgatccaagtgcaaatcctcccgcgcaagtgcctatcgatctggacatagacgagaacatcagtgcagatatgatcagggaaatgatgtggcacgagatgctccactaccaccctgaagttgttgcagcaatgagtgcccgatgatcttcaactgtccccaggaactttgcaggctcacttttttttcttcttctaaaagcctacggcgattatcgcacctattaagtaaccaagacgtgcaagacttatttcatgtatatatgagcgaaataagaacgttgttgtatccatatagttcatgttatggaccattacttggtgtatgcatttcacatttccactggacagttatgttgttgtactagttcatgatgagaagtgctattaaagtgaattcagt</dnaseqindica> |
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