Difference between revisions of "Os05g0557700"
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===Evolution=== | ===Evolution=== | ||
| − | + | Plants require large amounts of phosphate (Pi) to maintain growth and development .To cope with Pi starvation, plants have evolved multiple strategies to increase Pi availability.The Pi starvation signaling and regulatory system has been well studied in Arabidopsis (Arabidopsis thaliana; Bari et al., 2006; Schachtman and Shin, 2007), demonstrating that PHR1, SIZ1, PHOSPHATE-RESPONSIVE2 (PHO2), and miR399 are major players. PHR1, a MYB transcription factor, is a key regulator of Pi starvation signaling. PHR1 overexpression in Arabidopsis led to excessive Pi accumulation in shoots and activation of Pi starvation-induced gene expression (Rubio et al., 2001; Nilsson et al., 2007). SIZ1 is a small plant ubiquitin-like modifier (SUMO) E3 ligase that controls PHR1 sumoylation (Miura et al., 2005). miR399, the target gene of PHR1, is specifically induced by Pi starvation (Fujii et al., 2005) and can negatively regulate PHO2 expression through mRNA degradation (Chiou et al., 2006). Both miR399 overexpression and mutations in PHO2 resulted in overaccumulation of Pi in Arabidopsis shoots (Fujii et al., 2005; Chiou et al., 2006). Expression of several Pi starvation-induced genes such as Pht1;8, Pht1;9, AtIPS1, and AT4 was increased in pho2 mutants even under Pi-sufficient conditions, suggesting that PHO2 is involved in the regulation of Pi starvation responses (Bari et al., 2006). | |
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==Labs working on this gene== | ==Labs working on this gene== | ||
Revision as of 08:33, 6 June 2014
Please input one-sentence summary here.
Contents
Annotated Information
Function
Although phosphate (Pi) starvation signaling is well studied in Arabidopsis (Arabidopsis thaliana), it is still largely unknown in rice (Oryza sativa). In this work, a rice leaf tip necrosis1 (ltn1) mutant was identified and characterized. Map-based cloning identified LTN1 as LOC_Os05g48390, the putative ortholog of Arabidopsis PHO2, which plays important roles in Pi starvation signaling.Analysis of transgenic plants harboring a LTN1 promoter::β-glucuronidase construct revealed that LTN1 was preferentially expressed in vascular tissues. The ltn1 mutant exhibited increased Pi uptake and translocation, which led to Pi overaccumulation in shoots. In association with enhanced Pi uptake and transport, some Pi transporters were up-regulated in the ltn1 mutant in the presence of sufficient Pi.
Expression
Please input expression information here.
Evolution
Plants require large amounts of phosphate (Pi) to maintain growth and development .To cope with Pi starvation, plants have evolved multiple strategies to increase Pi availability.The Pi starvation signaling and regulatory system has been well studied in Arabidopsis (Arabidopsis thaliana; Bari et al., 2006; Schachtman and Shin, 2007), demonstrating that PHR1, SIZ1, PHOSPHATE-RESPONSIVE2 (PHO2), and miR399 are major players. PHR1, a MYB transcription factor, is a key regulator of Pi starvation signaling. PHR1 overexpression in Arabidopsis led to excessive Pi accumulation in shoots and activation of Pi starvation-induced gene expression (Rubio et al., 2001; Nilsson et al., 2007). SIZ1 is a small plant ubiquitin-like modifier (SUMO) E3 ligase that controls PHR1 sumoylation (Miura et al., 2005). miR399, the target gene of PHR1, is specifically induced by Pi starvation (Fujii et al., 2005) and can negatively regulate PHO2 expression through mRNA degradation (Chiou et al., 2006). Both miR399 overexpression and mutations in PHO2 resulted in overaccumulation of Pi in Arabidopsis shoots (Fujii et al., 2005; Chiou et al., 2006). Expression of several Pi starvation-induced genes such as Pht1;8, Pht1;9, AtIPS1, and AT4 was increased in pho2 mutants even under Pi-sufficient conditions, suggesting that PHO2 is involved in the regulation of Pi starvation responses (Bari et al., 2006).
Labs working on this gene
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References
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Structured Information
| Gene Name |
Os05g0557700 |
|---|---|
| Description |
The start codon is not identified. |
| Version |
NM_001062826.2 GI:297604894 GeneID:4339579 |
| Length |
7625 bp |
| Definition |
Oryza sativa Japonica Group Os05g0557700, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 5:27821672..27829296 |
| Sequence Coding Region |
27823037..27823285 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008398:27821672..27829296 source=RiceChromosome05 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008398:27821672..27829296 source=RiceChromosome05 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>tttcttgtacagcagttatatgatgttctttctttctattacacagatggtattcatgttagagtttatgaagaaaggatggacctcctgagggcatgccttgtaggtgcagctggaacaccttatcatgacaatcttttcttctttgatattttctttcctcctgattatcctcatgagccacctgtaagttcttcatttttcattgctcttacctacatcttctattccagattttcttgctgctga</cdnaseq> |
| Protein Sequence |
<aaseq>FLVQQLYDVLSFYYTDGIHVRVYEERMDLLRACLVGAAGTPYHD NLFFFDIFFPPDYPHEPPVSSSFFIALTYIFYSRFSCC</aaseq> |
| Gene Sequence |
<dnaseqindica>6012..6260#gcagcagcagcagcagcagaagcagaagcagtagaagaagaagcttttcttgctttgcttgctttctctctctctaggctccatctcctccctccaccactgcgtctcgaaatatccggctttcttgttgtgcgatttggtgcgaggtggtggtgattgcttcgtcggctgttgatttcttcgaggaattggagctgtgttgggggattcaggggggcttcatggtcgaagggctcggggatggattgaggcgtgggagaagaggagcttagcttcctcttcttcttcggtgatcgggtttggggttcttggaatcgcgaggaggcgggacatggcgggagattgggcttgtcacagactcaggtatggattgcattctcctctcgaatttggtttctttccggctgccctccgcgcagcccctgccccggcgaggctggccggcggcgactgccaccccgccgccggtgatgtgcgtgcaagaattgcgcttcaatggcgaatttccttgtgttgtctcgattttgaccatcacgtttacatggttaaaaatggtagtagtctttagaaaaaatggaagattcaggtgttcatcgttagctcttgatagcaatgcgtctgatttttttttctttttttgggtcagtctttgagacttaggattgttggattgggggagatttgtcatatatgagtactttgtgggtgctacgaattgttttcgcgccattttttcttctggtttttgggagagcgtgattagcacggaaggtagtttgttggatttgattagttacgtcggaaaggttaaatattagctgcttatgtcgactacgatgttgaaatgtctgagatttttctgtactgttgtttcaatcaagaaggcacccaaactggcaattccaagtaaaaaaaaactatgttttataacctactaggttcgtctaatttatgcatgattgatcatttcttgcgttaataatatgggcatgattttctgttgatgttttgttttcgctcgtatgttaatcctttagatatggactttcagatgtttccttgtagtattagttttgatttgacccctatctgtatgtggtcttttaatagtaaaatctaaccatataaatgtgcagatatttggagtaaaattcttctatttacatttctttcaacgactgttatctgaatttttttgcgtgcagtttgattttatactctcttttgcaagtaacgttaataatttttttaatcttaatagttttttgtgtcagtttaaagccttaagctggtttgtaagtaccaatgatttagaattttagatacttttactttcgattgactgttacgtagcaggatcatactgcagaatgctcatctgtgagctttctataggctctttactctctcttttaacatctgctatgctgtattgcaaactagctattcattatgtttcctgaataatgaaaatgtgtgtctgccggtctgcccctgtttggataagaaaatttcaagtactggcatataaaggaaaatggactatttcaggcaaatattcatttttgcttgttatgagacttatattattttactttagatacctttactatcaacaagtggtggttgattcatatcctacccttttttgtgtgtagagctgtaaatgcttaaccatgagcattgtagtatgtacatgagttagttccttgtagaactcaaattatcgtcaaatcatcatactaaagtatccgtacaagattcattgtacaaaacctttttttttggtaaaatttttttcgataatgttttggtaaaatttgcatcctatcaacaagcaacatgaaaccatgcatatacatatatgtagagttgtaaatgatcctttttttttatatcctcaaaaagaccaaaacgcacgcatatgtccagcacaaacatttgtagcaacatataaatctttatgaggttgatctttgtgcctaataatgccttttgatgagagtgttctctaaagcatctaagatactaatatttttctcaatttggattgtcgtgaccaattggactgaagttctcaccacatgtttgcagggcctgctgatcaatgcaaatagacttgaaagaacattatattctcgtgatcttatcaagtgaagccatatctaatgaaagcttgttatatttgttgctttcttgatgagatttgttgcttatgaccacttgtgtcctctaatcatccaaagcaaaaaaaatcattcagccggtctttacctctccaggaattaacacctgtgacaagttattccttgttttagggcaaatctcctttggcaattgcaccttcctgtcgatcgattatcctgagcatattcctcttattgagagaacccttcagctgggcaaatctcctttggcaaaatacctatctatagtctgtctattcgggcaaatctcctttggcatttagcttagattgcttccgggcaaatctcctttggcactacctttagcttcagattgctctttgggcaaatctcctttggcactaggtactctactactatccgcattagatatttggatcaaatatcctctggaagttagtggtattacatcattatttcctgcaactatatatcgtggtaattctcctttggcattagcactgtacacacattggttctaccacaatatcgatctcagtgaaagactgaacaccaggaagtccagaacgattcaaggtaatttgtcctcttttatggtgtatattttttatgtgagccgtcattttctataatatttattcacttacaaaagctttcctattttgactcaattttcagaaataatttttggctggtggccgttgtattttagcatcttggacaggaagctttcagattctgattgttcagtctggaagattgttttgttatttatcaactctatacatcagtgcgcctatcctaagaaggtgagcatgtagttaatagtatatttcctttaaatatttctgtgtctattccattatctaaaaaatatatatttctgtgtctgattccatgtgaaaaatgacaacgtactttgttttagggctcggaaagatggatctatatgcaattgactcggacactgagtcatacgctgaaactagtgacagtgaagaagaccaagaagaatgtgaacttacatactgtggtcatgcccagaacatcttgtccagcttggacgagagcattggaaagatcgataactttctaagttttgaaagaggatttctgcatggagacatagtttgctctgcagctgacccatcaggtcagctgggccgtgtagttggagttgatatgcttgttgacttggaaacgagctctggtgacataataaaacatgtcaacagcaagaaactttcaagagttagatcctttgtctctggtgattgtgtggtgatggggccatggattggacgggtgatcagggcatttgacctggtcactgttgtgttcagtgatggggcaaggtgtgaaatgctcttaagagattctgaggtactgaagcccattcctccaatcctttttgaagatgcaccttatttttactatccaggccaacgtgtgagaattgttcatccttccatttctaagtcgggtacatggttgtgtggatcatggaaagctagtagagatgaaggagtagtgtctcatgtcgacgttgggctggtacatgtgaactggatcacctcagtaacaaatgtttggggaggtcaatcatcaagtcctccaaactttcaggacccaaagaagctcactttgctttcatgcttcccttatgccaattggcagttgggtgactggtgcactctttcggattgcgagggaagtctctgggaaaattctgacaaatcttgcttcatgagtatgacctggaagtccagctccgacactcaaacggctatcggaacttatggttcagattattctcaaacatatgttgttgcaaagacaaagagtagtgttgatgttttatggcaagatggaagtacatctcttggtttagaacctcaaagtttggttcctgtgagtactcttggtgatcatgatttttggcctgggcaatttatccttgagaaattgacagttgaggataatggtagatgccagcggactgggatagtgacaagtgtggatgcacttgaacggactgtaaaggtaaaatgggctgtttcagttgatagtgacactgtcagttatggagatggactcactgaggagactgtgagtgcttatgagctagtattacacccagatttctccttcttcactggcgaagtcattattagatctgctgtcaacatagaaaattctgaagcaaatcttaccaatggaaccgtggcagttagcagggagagcttggatacctcctctgccttcttatcatgtattggcaatgttcttggatacaatgatgaaggacttgaagtccaatgggctagtggtgcgatatctagggtaagcttaactttgagaaactttcttctcttttttttgttgctttgatttattttatttacagaagtgttgctttactttataccagttttatttccaaactttgatgaaacatggatcggtccctggtcagactaatgataacataacttatcttgtacaaatttcaggttcagcattttgaaattattgcattagataggattctagatgattctctagaatccatgatcgaggagcacactactgatgatttggtagacatggctgagcaggagaaaatgcaccttgaggatactaaggtccgcttctgtctacaaattccattgtacggttattaaacttatgggttgaaatttcatcgatgatttatcattttcattcagagtgcgctggaagaatctgctggagactgtacaggatccttgcgcaaagccactgctttcctcttttccaagacagca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