Difference between revisions of "Os10g0403000"
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===Expression=== | ===Expression=== | ||
| − | + | PLA1 and PLA2 gene expression is associated with GA signaling. Thus, we examined the effect of GA on PLA1 and PLA2 gene expression. Ten-day-old seedlings were treated with 10 lM GA3 and PLA gene expression was monitored for 24 h by real-time PCR. PLA1 and PLA2 expression increased as | |
| + | early as 3 h after treatment, and a high level of expression was maintained for 24 h (Fig. 3a). To investigate the longterm effect of GA, wild-type seeds were inoculated and grown for 8 days on culture media containing 10 lM GA3. PLA1 and PLA2 expression was maintained at a high level for 8 days (Fig. 3b). Next, we examined the effect of uniconazole, a GA biosynthesis inhibitor. Uniconazole treatment markedly suppressed PLA1 and PLA2 gene expression (Fig. 3b). Therefore, GA regulates the expressions of both PLA1 and PLA2. | ||
===Evolution=== | ===Evolution=== | ||
Revision as of 17:17, 6 June 2014
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Contents
Annotated Information
Function
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Expression
PLA1 and PLA2 gene expression is associated with GA signaling. Thus, we examined the effect of GA on PLA1 and PLA2 gene expression. Ten-day-old seedlings were treated with 10 lM GA3 and PLA gene expression was monitored for 24 h by real-time PCR. PLA1 and PLA2 expression increased as early as 3 h after treatment, and a high level of expression was maintained for 24 h (Fig. 3a). To investigate the longterm effect of GA, wild-type seeds were inoculated and grown for 8 days on culture media containing 10 lM GA3. PLA1 and PLA2 expression was maintained at a high level for 8 days (Fig. 3b). Next, we examined the effect of uniconazole, a GA biosynthesis inhibitor. Uniconazole treatment markedly suppressed PLA1 and PLA2 gene expression (Fig. 3b). Therefore, GA regulates the expressions of both PLA1 and PLA2.
Evolution
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Labs working on this gene
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References
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Structured Information
| Gene Name |
Os10g0403000 |
|---|---|
| Description |
Cytochrome P450 78A11 |
| Version |
NM_001071087.1 GI:115481917 GeneID:4348574 |
| Length |
1754 bp |
| Definition |
Oryza sativa Japonica Group Os10g0403000, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 10:14016190..14017943 |
| Sequence Coding Region |
14016190..14016822,14016909..14017943 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008403:14016190..14017943 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008403:14016190..14017943 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggcaatggccaccgccaccgcctcctcctgcgtcgacgccacgtggtgggcgtacgccctcccggcgctcctcggcgccgacaccctctgcgcccacccggcgctgctcgccggcgccgtcctcctggccttcgccaccgccgcggtgctcgcctgggccgcgtcccccggcgggccggcgtgggcgcacggccgcggccgcctcggcgcgacgcccatcgaggggccccgggggctccccgtgttcggcagcatcttcgcgctctcccggggcctcccgcaccgcgcgctcgacgcgatgtcgcgcgacgcggcggcgccacgggcgagggagctcatggcgttctccgtcggggagacgccggcggtggtgtcgtcgtgcccggcgacggcgagggaggtgctcgcgcacccgtcgttcgccgaccgcccgctgaagcgctcggcgcgggagctgctgttcgcgcgcgccatcgggttcgcccccagcggcgagtactggcgcctcctccgccgcatcgcctccacccacctcttctcccctcgccgcgtcgccgcgcacgagccggggcgccaggccgacgccacggcgatgctgtccgccatggccgccgagcagtccgccaccggcgccgtcgtgctccgcccccacctccaggccgccgcgctcaacaacatcatgggcagcgtgttcggccggcgctacgacgtctcctcctcctccggcgccgccgccgacgaggccgagcagctcaagagcatggtgcgcgaggggttcgagctcctcggcgcgttcaactggtccgaccacctcccatggctcgcccacctctacgaccccaaccacgtcgcccgccgctgcgccgcgctcgtcccccgcgtccaggcgttcgtccgcggcgtcatccgcgaccaccgcctccgccgcgactcctcctccaccgccgccgacaatgccgacttcgtcgacgtcctcctctccctcgaggcccacgagaacctcgccgaggacgacatggtcgccgtcctctgggagatgatatttcgtgggacggacacgacggcgttggtgacggagtggtgcatggcggaggtggtgaggaacccggcggtgcaggcgaggctgagggcggaggtggacgcggcggtgggcggcgacgggtgtcccagcgacggcgacgtggcgcggatgccgtacctgcaggcggtggtgaaggagacgctgagggcgcacccgccggggccgctgctgagctgggcgcggctggccaccgccgacgtggggctcgccaacggcatggtggtgccggcgggcacgacggcgatggtgaacatgtgggccatcacccacgacggcgaggtgtgggccgacccggaggcgttcgcgccggagcggttcatcccgtcggagggcggcgccgacgtcgacgtccgcggcggcgacctccgcctggcgccgttcggcgccgggcgccgcgtctgccccggcaagaacctcggcctcgccaccgtcaccctctgggtcgcccgcctcgtccacgccttcgactggttcctccccgacggctcgccgccggtgtccctcgacgaggtcctcaagctctccctcgagatgaagacccctctcgccgccgccgccaccccccgccgccgccgcgccgcctga</cdnaseq> |
| Protein Sequence |
<aaseq>MAMATATASSCVDATWWAYALPALLGADTLCAHPALLAGAVLLA FATAAVLAWAASPGGPAWAHGRGRLGATPIEGPRGLPVFGSIFALSRGLPHRALDAMS RDAAAPRARELMAFSVGETPAVVSSCPATAREVLAHPSFADRPLKRSARELLFARAIG FAPSGEYWRLLRRIASTHLFSPRRVAAHEPGRQADATAMLSAMAAEQSATGAVVLRPH LQAAALNNIMGSVFGRRYDVSSSSGAAADEAEQLKSMVREGFELLGAFNWSDHLPWLA HLYDPNHVARRCAALVPRVQAFVRGVIRDHRLRRDSSSTAADNADFVDVLLSLEAHEN LAEDDMVAVLWEMIFRGTDTTALVTEWCMAEVVRNPAVQARLRAEVDAAVGGDGCPSD GDVARMPYLQAVVKETLRAHPPGPLLSWARLATADVGLANGMVVPAGTTAMVNMWAIT HDGEVWADPEAFAPERFIPSEGGADVDVRGGDLRLAPFGAGRRVCPGKNLGLATVTLW VARLVHAFDWFLPDGSPPVSLDEVLKLSLEMKTPLAAAATPRRRRAA</aaseq> |
| Gene Sequence |
<dnaseqindica>1122..1754#1..1035#atggcaatggccaccgccaccgcctcctcctgcgtcgacgccacgtggtgggcgtacgccctcccggcgctcctcggcgccgacaccctctgcgcccacccggcgctgctcgccggcgccgtcctcctggccttcgccaccgccgcggtgctcgcctgggccgcgtcccccggcgggccggcgtgggcgcacggccgcggccgcctcggcgcgacgcccatcgaggggccccgggggctccccgtgttcggcagcatcttcgcgctctcccggggcctcccgcaccgcgcgctcgacgcgatgtcgcgcgacgcggcggcgccacgggcgagggagctcatggcgttctccgtcggggagacgccggcggtggtgtcgtcgtgcccggcgacggcgagggaggtgctcgcgcacccgtcgttcgccgaccgcccgctgaagcgctcggcgcgggagctgctgttcgcgcgcgccatcgggttcgcccccagcggcgagtactggcgcctcctccgccgcatcgcctccacccacctcttctcccctcgccgcgtcgccgcgcacgagccggggcgccaggccgacgccacggcgatgctgtccgccatggccgccgagcagtccgccaccggcgccgtcgtgctccgcccccacctccaggccgccgcgctcaacaacatcatgggcagcgtgttcggccggcgctacgacgtctcctcctcctccggcgccgccgccgacgaggccgagcagctcaagagcatggtgcgcgaggggttcgagctcctcggcgcgttcaactggtccgaccacctcccatggctcgcccacctctacgaccccaaccacgtcgcccgccgctgcgccgcgctcgtcccccgcgtccaggcgttcgtccgcggcgtcatccgcgaccaccgcctccgccgcgactcctcctccaccgccgccgacaatgccgacttcgtcgacgtcctcctctccctcgaggcccacgagaacctcgccgaggacgacatggtcgccgtcctctgggtaaaaaaaaaaaaaaaaaaacaaattctactcaaacatttcaaactcaaatgtttttttaaaaatgtttttgtgtattttggcaggagatgatatttcgtgggacggacacgacggcgttggtgacggagtggtgcatggcggaggtggtgaggaacccggcggtgcaggcgaggctgagggcggaggtggacgcggcggtgggcggcgacgggtgtcccagcgacggcgacgtggcgcggatgccgtacctgcaggcggtggtgaaggagacgctgagggcgcacccgccggggccgctgctgagctgggcgcggctggccaccgccgacgtggggctcgccaacggcatggtggtgccggcgggcacgacggcgatggtgaacatgtgggccatcacccacgacggcgaggtgtgggccgacccggaggcgttcgcgccggagcggttcatcccgtcggagggcggcgccgacgtcgacgtccgcggcggcgacctccgcctggcgccgttcggcgccgggcgccgcgtctgccccggcaagaacctcggcctcgccaccgtcaccctctgggtcgcccgcctcgtccacgccttcgactggttcctccccgacggctcgccgccggtgtccctcgacgaggtcctcaagctctccctcgagatgaagacccctctcgccgccgccgccaccccccgccgccgccgcgccgcctga</dnaseqindica> |
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