Difference between revisions of "Os10g0403000"
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==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
| − | + | The molecular basis of plastochron regulation remains to be uncovered. plastochron 1 (pla1) is the first mutant that drastically alters plastochron, in which leaf primordia are formed approximately twofold faster than in the wild type. Concomitantly, leaves of pla1 become short, suggesting that PLA1 regulates organ size. The PLA1 gene encodes a cytochrome P450 family protein (CYP78A11). An Arabidopsis homolog of PLA1, KLUH, was shown to regulate organ size. Subsequently, the PLA2 and PLA3 genes, loss-of-function mutants of which exhibit similar phenotypes to that of pla1, were identified. These encode an RNA-binding protein and glutamate carboxypeptidase, respectively. Interestingly, PLA1 and PLA2 are expressed in young leaf primordia, but not in shoot meristems. Therefore, based on analyses of the developmental processes of leaves, the primary functions of PLA1 and PLA2 are suppression of precocious leaf maturation, and that some non-cell autonomous signals move from leaf primordial through shoot meristems to suppress the formation of a new leaf primordium. Thus, PLA1 and PLA2 are key genes for elucidating leaf development. | |
| − | |||
===Expression=== | ===Expression=== | ||
PLA1 and PLA2 gene expression is associated with GA signaling. Thus, we examined the effect of GA on PLA1 and PLA2 gene expression. Ten-day-old seedlings were treated with 10 lM GA3 and PLA gene expression was monitored for 24 h by real-time PCR. PLA1 and PLA2 expression increased as | PLA1 and PLA2 gene expression is associated with GA signaling. Thus, we examined the effect of GA on PLA1 and PLA2 gene expression. Ten-day-old seedlings were treated with 10 lM GA3 and PLA gene expression was monitored for 24 h by real-time PCR. PLA1 and PLA2 expression increased as | ||
Revision as of 17:22, 6 June 2014
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Contents
Annotated Information
Function
The molecular basis of plastochron regulation remains to be uncovered. plastochron 1 (pla1) is the first mutant that drastically alters plastochron, in which leaf primordia are formed approximately twofold faster than in the wild type. Concomitantly, leaves of pla1 become short, suggesting that PLA1 regulates organ size. The PLA1 gene encodes a cytochrome P450 family protein (CYP78A11). An Arabidopsis homolog of PLA1, KLUH, was shown to regulate organ size. Subsequently, the PLA2 and PLA3 genes, loss-of-function mutants of which exhibit similar phenotypes to that of pla1, were identified. These encode an RNA-binding protein and glutamate carboxypeptidase, respectively. Interestingly, PLA1 and PLA2 are expressed in young leaf primordia, but not in shoot meristems. Therefore, based on analyses of the developmental processes of leaves, the primary functions of PLA1 and PLA2 are suppression of precocious leaf maturation, and that some non-cell autonomous signals move from leaf primordial through shoot meristems to suppress the formation of a new leaf primordium. Thus, PLA1 and PLA2 are key genes for elucidating leaf development.
Expression
PLA1 and PLA2 gene expression is associated with GA signaling. Thus, we examined the effect of GA on PLA1 and PLA2 gene expression. Ten-day-old seedlings were treated with 10 lM GA3 and PLA gene expression was monitored for 24 h by real-time PCR. PLA1 and PLA2 expression increased as early as 3 h after treatment, and a high level of expression was maintained for 24 h (Fig. 3a). To investigate the longterm effect of GA, wild-type seeds were inoculated and grown for 8 days on culture media containing 10 lM GA3. PLA1 and PLA2 expression was maintained at a high level for 8 days (Fig. 3b). Next, we examined the effect of uniconazole, a GA biosynthesis inhibitor. Uniconazole treatment markedly suppressed PLA1 and PLA2 gene expression (Fig. 3b). Therefore, GA regulates the expressions of both PLA1 and PLA2.
Evolution
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Labs working on this gene
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References
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Structured Information
| Gene Name |
Os10g0403000 |
|---|---|
| Description |
Cytochrome P450 78A11 |
| Version |
NM_001071087.1 GI:115481917 GeneID:4348574 |
| Length |
1754 bp |
| Definition |
Oryza sativa Japonica Group Os10g0403000, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 10:14016190..14017943 |
| Sequence Coding Region |
14016190..14016822,14016909..14017943 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008403:14016190..14017943 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008403:14016190..14017943 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggcaatggccaccgccaccgcctcctcctgcgtcgacgccacgtggtgggcgtacgccctcccggcgctcctcggcgccgacaccctctgcgcccacccggcgctgctcgccggcgccgtcctcctggccttcgccaccgccgcggtgctcgcctgggccgcgtcccccggcgggccggcgtgggcgcacggccgcggccgcctcggcgcgacgcccatcgaggggccccgggggctccccgtgttcggcagcatcttcgcgctctcccggggcctcccgcaccgcgcgctcgacgcgatgtcgcgcgacgcggcggcgccacgggcgagggagctcatggcgttctccgtcggggagacgccggcggtggtgtcgtcgtgcccggcgacggcgagggaggtgctcgcgcacccgtcgttcgccgaccgcccgctgaagcgctcggcgcgggagctgctgttcgcgcgcgccatcgggttcgcccccagcggcgagtactggcgcctcctccgccgcatcgcctccacccacctcttctcccctcgccgcgtcgccgcgcacgagccggggcgccaggccgacgccacggcgatgctgtccgccatggccgccgagcagtccgccaccggcgccgtcgtgctccgcccccacctccaggccgccgcgctcaacaacatcatgggcagcgtgttcggccggcgctacgacgtctcctcctcctccggcgccgccgccgacgaggccgagcagctcaagagcatggtgcgcgaggggttcgagctcctcggcgcgttcaactggtccgaccacctcccatggctcgcccacctctacgaccccaaccacgtcgcccgccgctgcgccgcgctcgtcccccgcgtccaggcgttcgtccgcggcgtcatccgcgaccaccgcctccgccgcgactcctcctccaccgccgccgacaatgccgacttcgtcgacgtcctcctctccctcgaggcccacgagaacctcgccgaggacgacatggtcgccgtcctctgggagatgatatttcgtgggacggacacgacggcgttggtgacggagtggtgcatggcggaggtggtgaggaacccggcggtgcaggcgaggctgagggcggaggtggacgcggcggtgggcggcgacgggtgtcccagcgacggcgacgtggcgcggatgccgtacctgcaggcggtggtgaaggagacgctgagggcgcacccgccggggccgctgctgagctgggcgcggctggccaccgccgacgtggggctcgccaacggcatggtggtgccggcgggcacgacggcgatggtgaacatgtgggccatcacccacgacggcgaggtgtgggccgacccggaggcgttcgcgccggagcggttcatcccgtcggagggcggcgccgacgtcgacgtccgcggcggcgacctccgcctggcgccgttcggcgccgggcgccgcgtctgccccggcaagaacctcggcctcgccaccgtcaccctctgggtcgcccgcctcgtccacgccttcgactggttcctccccgacggctcgccgccggtgtccctcgacgaggtcctcaagctctccctcgagatgaagacccctctcgccgccgccgccaccccccgccgccgccgcgccgcctga</cdnaseq> |
| Protein Sequence |
<aaseq>MAMATATASSCVDATWWAYALPALLGADTLCAHPALLAGAVLLA FATAAVLAWAASPGGPAWAHGRGRLGATPIEGPRGLPVFGSIFALSRGLPHRALDAMS RDAAAPRARELMAFSVGETPAVVSSCPATAREVLAHPSFADRPLKRSARELLFARAIG FAPSGEYWRLLRRIASTHLFSPRRVAAHEPGRQADATAMLSAMAAEQSATGAVVLRPH LQAAALNNIMGSVFGRRYDVSSSSGAAADEAEQLKSMVREGFELLGAFNWSDHLPWLA HLYDPNHVARRCAALVPRVQAFVRGVIRDHRLRRDSSSTAADNADFVDVLLSLEAHEN LAEDDMVAVLWEMIFRGTDTTALVTEWCMAEVVRNPAVQARLRAEVDAAVGGDGCPSD GDVARMPYLQAVVKETLRAHPPGPLLSWARLATADVGLANGMVVPAGTTAMVNMWAIT HDGEVWADPEAFAPERFIPSEGGADVDVRGGDLRLAPFGAGRRVCPGKNLGLATVTLW VARLVHAFDWFLPDGSPPVSLDEVLKLSLEMKTPLAAAATPRRRRAA</aaseq> |
| Gene Sequence |
<dnaseqindica>1122..1754#1..1035#atggcaatggccaccgccaccgcctcctcctgcgtcgacgccacgtggtgggcgtacgccctcccggcgctcctcggcgccgacaccctctgcgcccacccggcgctgctcgccggcgccgtcctcctggccttcgccaccgccgcggtgctcgcctgggccgcgtcccccggcgggccggcgtgggcgcacggccgcggccgcctcggcgcgacgcccatcgaggggccccgggggctccccgtgttcggcagcatcttcgcgctctcccggggcctcccgcaccgcgcgctcgacgcgatgtcgcgcgacgcggcggcgccacgggcgagggagctcatggcgttctccgtcggggagacgccggcggtggtgtcgtcgtgcccggcgacggcgagggaggtgctcgcgcacccgtcgttcgccgaccgcccgctgaagcgctcggcgcgggagctgctgttcgcgcgcgccatcgggttcgcccccagcggcgagtactggcgcctcctccgccgcatcgcctccacccacctcttctcccctcgccgcgtcgccgcgcacgagccggggcgccaggccgacgccacggcgatgctgtccgccatggccgccgagcagtccgccaccggcgccgtcgtgctccgcccccacctccaggccgccgcgctcaacaacatcatgggcagcgtgttcggccggcgctacgacgtctcctcctcctccggcgccgccgccgacgaggccgagcagctcaagagcatggtgcgcgaggggttcgagctcctcggcgcgttcaactggtccgaccacctcccatggctcgcccacctctacgaccccaaccacgtcgcccgccgctgcgccgcgctcgtcccccgcgtccaggcgttcgtccgcggcgtcatccgcgaccaccgcctccgccgcgactcctcctccaccgccgccgacaatgccgacttcgtcgacgtcctcctctccctcgaggcccacgagaacctcgccgaggacgacatggtcgccgtcctctgggtaaaaaaaaaaaaaaaaaaacaaattctactcaaacatttcaaactcaaatgtttttttaaaaatgtttttgtgtattttggcaggagatgatatttcgtgggacggacacgacggcgttggtgacggagtggtgcatggcggaggtggtgaggaacccggcggtgcaggcgaggctgagggcggaggtggacgcggcggtgggcggcgacgggtgtcccagcgacggcgacgtggcgcggatgccgtacctgcaggcggtggtgaaggagacgctgagggcgcacccgccggggccgctgctgagctgggcgcggctggccaccgccgacgtggggctcgccaacggcatggtggtgccggcgggcacgacggcgatggtgaacatgtgggccatcacccacgacggcgaggtgtgggccgacccggaggcgttcgcgccggagcggttcatcccgtcggagggcggcgccgacgtcgacgtccgcggcggcgacctccgcctggcgccgttcggcgccgggcgccgcgtctgccccggcaagaacctcggcctcgccaccgtcaccctctgggtcgcccgcctcgtccacgccttcgactggttcctccccgacggctcgccgccggtgtccctcgacgaggtcctcaagctctccctcgagatgaagacccctctcgccgccgccgccaccccccgccgccgccgcgccgcctga</dnaseqindica> |
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