Difference between revisions of "Os03g0666200"
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===Expression=== | ===Expression=== | ||
| − | + | The interaction ''in vivo'' between OsSWAP70A and OsRac1 in rice protoplasts were analysed by a bimolecular fluorescence complementation (BiFC) assay (Figure 2b). OsRac1 tagged with the N-terminal domain [amino acids (aa) 1–154] of Venus (Vn-OsRac1) and OsSWAP70A tagged with the C-terminal domain (aa 155–238) (OsSWAP70A-Vc) were co-expressed in rice protoplasts together with the control red fluorescent protein (RFP). The Venus fluorescence was detected at the plasma membrane, whereas RFP was observed throughout the cytoplasm. These results indicate that OsRac1 and OsSWAP70A interacted with each other at the plasma membrane. | |
===Evolution=== | ===Evolution=== | ||
Revision as of 01:51, 7 June 2014
SWAP-70, an unusual phosphatidylinositol-3-kinase-dependent protein that interacts with the RhoGTPase Rac.
Contents
Annotated Information
Function
SWAP70 has been shown to exhibit GEF activities toward Cdc42, Rac, and Rho. In the present study, we found that rice [Oryza sativa (Os)] SWAP70 interacted with OsRac1 and the DH domain exhibited GEF activity toward OsRac1 in vitro, evidence that plants possess a functional DH domain. In addition, OsSWAP70 regulates chitin-induced ROS production and defense gene expression in rice. Thus, it is likely that SWAP70 functions as a GEF for Rac/Rop GTPases in rice.
Expression
The interaction in vivo between OsSWAP70A and OsRac1 in rice protoplasts were analysed by a bimolecular fluorescence complementation (BiFC) assay (Figure 2b). OsRac1 tagged with the N-terminal domain [amino acids (aa) 1–154] of Venus (Vn-OsRac1) and OsSWAP70A tagged with the C-terminal domain (aa 155–238) (OsSWAP70A-Vc) were co-expressed in rice protoplasts together with the control red fluorescent protein (RFP). The Venus fluorescence was detected at the plasma membrane, whereas RFP was observed throughout the cytoplasm. These results indicate that OsRac1 and OsSWAP70A interacted with each other at the plasma membrane.
Evolution
SWAP70 protein possessed a PH domain at the N-terminus of the putative DH domain, a characteristic of human SWAP70 Rho GEF (Shinohara et al., 2002). Both the putative PH and DH domains had 22% identity and 64% similarity to human SWAP70 (Figure 1,2).
The PH domain contained four basic amino acids conserved in the PH domains of animal SWAP70 proteins, which are critical for binding to phosphatidylinositol 3,4,5-triphosphate (PIP3) (Oka et al., 2007). We also found an additional SWAP70 homologous gene (Os07g0138100) in rice by a BLAST search. Therefore, Os03g0666200 and Os07g0138100 were named OsSWAP70A and OsSWAP70B, respectively. OsSWAP70A and OsSWAP70B shared 64.2% identity (data not shown). A BLAST-based search for plant SWAP70 indicated that maize and sorghum have two genes, and there is one gene in Arabidopsis, Vitis vinifera, Ricinus communis, and Populus trichocarpa (Figure 3), suggesting that the SWAP70 homologs are conserved in the plant kingdom.
Labs working on this gene
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References
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Structured Information
| Gene Name |
Os03g0666200 |
|---|---|
| Description |
Pleckstrin homology-type domain containing protein |
| Version |
NM_001057380.1 GI:115454488 GeneID:4333661 |
| Length |
3925 bp |
| Definition |
Oryza sativa Japonica Group Os03g0666200, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 3:26968125..26972049 |
| Sequence Coding Region |
26968410..26968770,26968898..26969190,26969267..26969494,26970120..26970351,26970574..26970655 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008396:26968125..26972049 source=RiceChromosome03 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008396:26968125..26972049 source=RiceChromosome03 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggccaccaacggcagctccccgagggcgagggagactgagagcagcctggagaaggtgaagcggcagctctcgtcggggtccgggcggtacctgctgcaggggccactgctgaagcgatctgagacgttgaggaaatggaatgagagatgggtaattttagaccctacttcaggaaagatggaatacaaaattcggagaaatgaaactgccattaaaggaaccattatatttgatgcttcaagcactattaccttgtcccctgtaaatttccaagggatgccaaagtatgatggctgctgtttctacattggaactcctcaaaaaaaggattactttctttgtgctgaaactcctggtgctgcaaaggcttgggtatctacgttgcttgcaacacaattagtactacaagcgcataaagaagcagtgaattctttggctgggaacggctctcctgccacgttaggtacagttgctacagcagttgctaatgccaacgctactgctttggaggctaccaaggagatagaagcagcaatgaaagtttcaatgagggcagctcttgggttgggtgcaaacaatccaaaggaaggtcaacttgatgatttaaccatcatgaaggagactcttcgggtgaaagatgaggagttgcagaatttggccaaggatattcgtgctcgggatgctacaattaaggaaatagcaaataaattaacagagactgcagaggctgcggaagctgctgcttctgcagcccatacgatggatgaacagagaagacttctatgttcggagatcgagcgcctaagacaagcaatggaaagacaaatggaacaatccatgctcaagctaagacaatctgaagaaaaggtaattagcttgagtaaagagaaggaccaattgctgaaggaaagagatgctgcacttcaggaggctcatatgtggcgcactgagctagggaaagctagagagcaagcagtaatacaagaagcaactattgccagagcagatgagaaggtaagggcatctgaagcagatgctgcagctcggataaaggaagctgctgaaaagttacatgctgttgagaaagaaaaagaggagcttctatcccttgttggtattcttcaatcacaagtccagagagagcaaagcagcacgaagcaggtatgcgaagagagatctgaatcatgctccggcaccgataactcccctcccttgacaaagcacgtcgatgcatcagatgacgatgtcgacaaagcttgtgtgagtgactcaagatctgtcctagtttccaatgataacactgaagtccaactggccgtggatggggtcgacatccgtcctatcggtgatgcggagtggggtagtttccagcagtcggaagcattgatcgccgatgtgcgggaggtctccccagaatcagaaggcggcagcttggatatccctgtggtcaaccccccaccagtgagcgatcatatccagggaggcgcaacccacccctga</cdnaseq> |
| Protein Sequence |
<aaseq>MATNGSSPRARETESSLEKVKRQLSSGSGRYLLQGPLLKRSETL RKWNERWVILDPTSGKMEYKIRRNETAIKGTIIFDASSTITLSPVNFQGMPKYDGCCF YIGTPQKKDYFLCAETPGAAKAWVSTLLATQLVLQAHKEAVNSLAGNGSPATLGTVAT AVANANATALEATKEIEAAMKVSMRAALGLGANNPKEGQLDDLTIMKETLRVKDEELQ NLAKDIRARDATIKEIANKLTETAEAAEAAASAAHTMDEQRRLLCSEIERLRQAMERQ MEQSMLKLRQSEEKVISLSKEKDQLLKERDAALQEAHMWRTELGKAREQAVIQEATIA RADEKVRASEADAAARIKEAAEKLHAVEKEKEELLSLVGILQSQVQREQSSTKQVCEE RSESCSGTDNSPPLTKHVDASDDDVDKACVSDSRSVLVSNDNTEVQLAVDGVDIRPIG DAEWGSFQQSEALIADVREVSPESEGGSLDIPVVNPPPVSDHIQGGATHP</aaseq> |
| Gene Sequence |
<dnaseqindica>3280..3640#2860..3152#2556..2783#1699..1930#1395..1476#1285..1316#1121..1204#973..1034#229..333#115..138#ggtcgacgccaccccccaccggccggccccgtctcgccgtcgccgccgtcgtctccgccacccaaaaccctagccctgcagcctcccctccgcagctcgccccctctcctcgccatggccaccaacggcagctccccggtacgacgacgacccgggatgtttctgggactttttcccccgatttccggcgattcttatgagagtttttttttcttttttgggttgcagagggcgagggagactgagagcagcctggagaaggtgaagcggcagctctcgtcggggtccgggcggtacctgctgcaggggccactgctgaagcgatctgagacggtatggtctcgccggtcgtatttcgtgagcgggtgtgatgggattgtatggggggtgtttattaggcgaggtttttggtgataggtttgatctatgaggtttttctggtgttgaatggttcgatttgagtctgcccttgagaattcttgtgtgcgagtattagggattttgatgtgtggttggttggtggtaggagaaattatggttcgattatgtgtgtagctattctgttcgatttcttggaatgatttttgttgaagtgatagggagtatgtgagatttggtgagtaagagctaggagcatcgcattgacctttgtcggtggcatcgtccaagtaaccatgttgtggtatttgagtctagtgtgatagactagagagaaaggggaagccaggtgaatcttattccaagggtagaatgaactttattttgagataaacataagtcatagggcgcctgccgttgatacattggtgcacacgaatttgctactgaccaggaaaatacaatctagtgaatcgactaggaccaattctgttcaaacaaatgcaatcagtggaaactagcaaacatagtttgttctgaaatgtgaaaatcacggcagcaatctaacctcttcgatttttttccgtcgtacagttgaggaaatggaatgagagatgggtaattttagaccctacttcaggaaagatggaatacaagtatggagaactttgaaagttttgtgactaaataccaattaaattcctgctattttctctgtactcatttttttcccttgttgcagaattcggagaaatgaaactgccattaaaggaaccattatatttgatgcttcaagcactattaccttgtcccctgtaaatttccagtaagattatctactgtatataaacttttcttggtatggtatttcgaagtttgaactaactactgctcaaattcttacagagggatgccaaagtatgatggctgctgtttctgtatcctttacacttgctcaatgtttttttaaggagtgtatatgcttttagtttttagtgttccttgacttcagatagacattggaactcctcaaaaaaaggattactttctttgtgctgaaactcctggtgctgcaaaggcttgggtatctacgttgctgtaagctttctgatccatgacttgtcaaatattgtcatactcccagattgctgcccactgccagtcatttacaaatgctaattcctaaacattactgcaaacacacatactccaatatgaacgggcaaatgaatgcgatgtattttctctctctctgaaaaaggtgtaggcaggagaattgctaccactctatcttgggctgatattatctattttaaacagtgcaacacaattagtactacaagcgcataaagaagcagtgaattctttggctgggaacggctctcctgccacgttaggtacagttgctacagcagttgctaatgccaacgctactgctttggaggctaccaaggagatagaagcagcaatgaaagtttcaatgagggcagctcttgggttgggtgcaaacaatccaaaggaaggtcaacttgatgatttaaccatcatgaaggtatgcattcttttctcagcgaagatagattctctcatctaaaatcttgtttcatattgcaagtggtcttgctcttctagataatctttactttgctgctttcaaatattttctgtgataacatttctattttattagttttaaatcgtatggtgtcccatctctacctatgaagcagaccacactctagtattcgcacttggtactgatttcatacagggcattacgaactgcagacaatattcctacatgctttacatcgttgctgtcaacctgtgctagtgtaatattaggttggttgcctcaggctagattgccagattaatatattatgtataaacttggtgcaaatttatgccacagaacatacctggttaattataagtagatgagatttggcttttattgggagtctgaaaattgttgtaatattgtatgacataaatttacactgtttggtatgatttgttcaacaatgttttccatttctaaacattttagatggttacatctttgcttattaggtttcaattttcatgcatgtgctgctaggcacatggttgggtatctttgattttattcatgagcattattttgtcaattttctacttttcatattcctcaggagactcttcgggtgaaagatgaggagttgcagaatttggccaaggatattcgtgctcgggatgctacaattaaggaaatagcaaataaattaacagagactgcagaggctgcggaagctgctgcttctgcagcccatacgatggatgaacagagaagacttctatgttcggagatcgagcgcctaagacaagcaatggaaagacaaatggaacaatccatgctcaaggtaaatagttatatttaatatctgaatttcgaatgctctgaattctcatgctccaaatggtctatggatattacagctaagacaatctgaagaaaaggtaattagcttgagtaaagagaaggaccaattgctgaaggaaagagatgctgcacttcaggaggctcatatgtggcgcactgagctagggaaagctagagagcaagcagtaatacaagaagcaactattgccagagcagatgagaaggtaagggcatctgaagcagatgctgcagctcggataaaggaagctgctgaaaagttacatgctgttgagaaagaaaaagaggagcttctatcccttgttggtattcttcaatcacaagtccagaggtttgtcttgttttatgtgttctgcatttactgagttaaaagagaaatttatataagagatggaagcatctcacgagttatgcattacaacattgtcattgacctaaacacgaacctactgatgcagagagcaaagcagcacgaagcaggtatgcgaagagagatctgaatcatgctccggcaccgataactcccctcccttgacaaagcacgtcgatgcatcagatgacgatgtcgacaaagcttgtgtgagtgactcaagatctgtcctagtttccaatgataacactgaagtccaactggccgtggatggggtcgacatccgtcctatcggtgatgcggagtggggtagtttccagcagtcggaagcattgatcgccgatgtgcgggaggtctccccagaatcagaaggcggcagcttggatatccctgtggtcaaccccccaccagtgagcgatcatatccagggaggcgcaacccacccctgaaggcctggagccaaatacattctgggattgttgtgattattgaggataaccgactgggatttgaatcctttcgagaattgctgtggcaggagttcgcaccggtgcctggttgtatgttcattgtattaaaactatacgactcaaataaaagatagcggtggcttgtgtgtgtaaattgttcggttcaagctctcttttgttacattctgacttcaatgcattaaagtagtagtaactgttaaaacggcagattagcagtgtgaatgtgagatcttgtcaacttgg</dnaseqindica> |
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