Difference between revisions of "Os02g0666200"
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The partial identification of Physcomitrella aquaporins demonstrates that the diversification into PIP, TIP, NIP and SIP subfamilies, as well as the differentiation into PIP1 and PIP2 classes, pre-dates the divergence of bryophytes and tracheophytes, whereas the differentiation of α-, γ- and δ-TIPs must have occurred during the evolution of tracheophytes. Identification of aquaporins in charophytes should elucidate to what degree the plant aquaporins had diversified before the algal ancestors invaded the land.<ref name="ref3" /> | The partial identification of Physcomitrella aquaporins demonstrates that the diversification into PIP, TIP, NIP and SIP subfamilies, as well as the differentiation into PIP1 and PIP2 classes, pre-dates the divergence of bryophytes and tracheophytes, whereas the differentiation of α-, γ- and δ-TIPs must have occurred during the evolution of tracheophytes. Identification of aquaporins in charophytes should elucidate to what degree the plant aquaporins had diversified before the algal ancestors invaded the land.<ref name="ref3" /> | ||
| − | [[File:diversification.png| | + | |
| + | [[File:diversification.png|600px|"diversification of aquaporins (from reference <ref name="ref3"/>)."]] | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
Revision as of 07:06, 7 June 2014
One of most highly expressed aquaporin gene in the leaves and roots of rice, highly responsible to environmental stresses.[1]
Contents
Annotated Information
Introduction
According to the nomenclature of PIP genes in maize, this gene has been designated as OsPIP1-1. PIP genes are the protein encoding genes of plasma membrane intrinsic proteins (PIPs) -- a subfamily of aquaporins that enable fast and controlled translocation of water across the membrane. There are recently 10 designated PIP genes in the gnome of rice, they are classified into two groups, that is OsPIP1-1 to OsPIP1-3 and OsPIP2-1 to OsPIP2-7, based on the similarity of their amino acid sequences.[2] The ubiquitously expression of OsPIP1-1 gene in rice leaves and roots indicates its important physiological role.
Function
Experiments show that when transiently expressed as EGFP-OsPIP1 in rice cell protoplasts, it is mainly distributed in cytoplasm, While co-expressing with EGFP-OsPIP1 make it re -translocate to plasma membrane. This observation is further confirmed by the water permeability measuring experiment.[1]
Seed germination
Transgenic rice seeds with overexpressed OsPIP1 exhibit a significant higher germination rate than the control, this is consistent with the higher α-amylase activity in transgenic seeds.[1]
Salt tolerance
Experiments show that when not overexpressed at high level,OsPIP1 will efficiently promote salt resistance in rice, but when has high OsPIP1 expression level, rice will become more sensitive to high salt environment than control.[1]
Seed yield
Transgenic rice plants with various OsPIP1 express level show that, appropriate low level of overexpressed OsPIP1 will increase seed yield, while high level of OsPIP1 expression will decrease seed yield, and extremely high expression will even make rice sterile.[1]
Expression
From the data in 2006, we can see OsPIP1 is highly expressed in both the roots and leavels of rice, with transcript copy numbers more than 10^3.[2]
![(transcript copy numbers of each PIP gene in rice (from reference [2])."](https://ngdc.cncb.ac.cn/ricewiki/index.php/File:2.jpg)
Evolution
Aquaporin family occur in greatest numbers and with the greatest diversity in plants. Angiosperm species have ~30 different aquaporins grouped into four subfamilies: plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin 26-like intrinsic proteins (NIPs), and small, basic intrinsic proteins (SIPs). An EST library of the moss Physcomitrella patens reveals that its aquaporins fall into the same subfamilies, indicating that the main radiation of plant aquaporins was already established when land plant evolution began.[3]
The partial identification of Physcomitrella aquaporins demonstrates that the diversification into PIP, TIP, NIP and SIP subfamilies, as well as the differentiation into PIP1 and PIP2 classes, pre-dates the divergence of bryophytes and tracheophytes, whereas the differentiation of α-, γ- and δ-TIPs must have occurred during the evolution of tracheophytes. Identification of aquaporins in charophytes should elucidate to what degree the plant aquaporins had diversified before the algal ancestors invaded the land.[3]
!["diversification of aquaporins (from reference [3])."](https://ngdc.cncb.ac.cn/ricewiki/index.php/File:Diversification.png)
Labs working on this gene
Graduate School of Bioresource Sciences, Akita Prefectural University, Shimoshinjo, Akita 010-0195, Japan
Institute of Plant Science and Resources, Okayama University, Kuashiki 710-0046, Japan
College of Life Science, Northeast Agricultural University, Harbin 150030, China
Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, National Laboratory for Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
References
Structured Information
| Gene Name |
Os02g0666200 |
|---|---|
| Description |
Aquaporin |
| Version |
NM_001054207.1 GI:115447784 GeneID:4330248 |
| Length |
3041 bp |
| Definition |
Oryza sativa Japonica Group Os02g0666200, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 2:27931202..27934242 |
| Sequence Coding Region |
27931313..27931649,27932641..27932936,27933296..27933436,27933924..27934019 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008395:27931202..27934242 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008395:27931202..27934242 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggaggggaaggaggaggacgtgcggctgggggcgaacaggtactcggagaggcagccgatagggacggcggcgcagggcgccggggacgacaaggactacaaggagccgccgccggcgccgctgttcgagccaggggagctcaagtcgtggtctttctaccgggccgggatcgccgagttcgtcgccaccttcctcttcctctacatcaccatcctcaccgtcatgggggtctccaagtcctcctccaagtgcgccaccgtcggcatccagggcatcgcctggtccttcggaggcatgatcttcgcgctcgtctactgcaccgccggcatctccggaggacacatcaacccagcagttacttttgggctgttcttggccaggaagctgtccctgacccgggccatcttctacatagtgatgcaatgcctaggggccatctgcggagctggagttgtgaagggcttccagcagggtctgtacatgggcaatggcggtggtgccaatgtagttgccagtggctacaccaagggtgacggtcttggtgctgagattgttggcaccttcatcctggtctacaccgtcttctcagccactgatgccaagaggaatgccagggactcacatgttcctatccttgccccactgccaattggttttgcggtgttcctggtccacctggccaccatccccatcaccggtactggcatcaacccagccaggagccttggcgctgccatcatctacaacaaggaccatgcctggaatgaccattggatcttctgggttggtcccttcgttggcgctgccctggctgccatctaccaccaggtgatcatcagggcgatcccattcaagagcaggtcttaa</cdnaseq> |
| Protein Sequence |
<aaseq>MEGKEEDVRLGANRYSERQPIGTAAQGAGDDKDYKEPPPAPLFE PGELKSWSFYRAGIAEFVATFLFLYITILTVMGVSKSSSKCATVGIQGIAWSFGGMIF ALVYCTAGISGGHINPAVTFGLFLARKLSLTRAIFYIVMQCLGAICGAGVVKGFQQGL YMGNGGGANVVASGYTKGDGLGAEIVGTFILVYTVFSATDAKRNARDSHVPILAPLPI GFAVFLVHLATIPITGTGINPARSLGAAIIYNKDHAWNDHWIFWVGPFVGAALAAIYH QVIIRAIPFKSRS</aaseq> |
| Gene Sequence |
<dnaseqindica>112..448#1440..1735#2095..2235#2723..2818#ttcttcttcagtgtactctgcctttataacaccctactcctctctctcacctccaccatctagctcactcacacagtctccactcacacgcattgcagaggagaggcgacaatggaggggaaggaggaggacgtgcggctgggggcgaacaggtactcggagaggcagccgatagggacggcggcgcagggcgccggggacgacaaggactacaaggagccgccgccggcgccgctgttcgagccaggggagctcaagtcgtggtctttctaccgggccgggatcgccgagttcgtcgccaccttcctcttcctctacatcaccatcctcaccgtcatgggggtctccaagtcctcctccaagtgcgccaccgtcggcatccagggcatcgcctggtccttcggaggcatgatcttcgcgctcgtctactgcaccgccggcatctccggtcagttcactctcctttggcttcctcccccaccttcaagatctcaagtcttcaactttgtcatgattttgccttggtttgtcttgctagttgatacttacttgttacctgcactttgcttggctcagtagtttcttcatactagcatgctcactttagtaaagcatgaggtatagaatcatgtgtactactactgctccttccataagtagcaagactttgaggcatatggcattaacactgaataattgatgaaagacttttagttacctgcttttggggtgttagatcagtattagtaactggaaattcactaaatttgttgtccatgctcttgtagttttaaagaattattatagctttctacaggtgttgaatgcaccaaaaatttatgacactaccttttgctcttgtaccatcatgtaccttgtctgcgcaaagatttgtcgctggcatattgtactgctgtggtatgtgaattttgttcttatgcaaaggtttgatttacgactagaagattaaggatttaaggtcatccgattgatttaactcttaacccagcattttcttcggtagtgttctaactgttgatgctgaaaccacacaaattatcacacaaaaatatattatttttagttcatcaaaattgaatgtgcacttggagccttggaggcaaagaaatatgtgatgccaaaattttctccttggttaatcttcctaccagaatattaacaaaactatatgaacaagtaaatatcttgtgttgtcaacttatacagtctatcacaactcgcaatcttagaagttaatttctgtaccagttttatataatgcttcaaaatctcaaatactaccttgttaatctcaaaatatgaatacatgagggtacactatacagtcttacttcaactgtcatgacaactgaccacaaaacaactacggcaattaagggcactactaatatccattatattgtctgttactgcaacaggaggacacatcaacccagcagttacttttgggctgttcttggccaggaagctgtccctgacccgggccatcttctacatagtgatgcaatgcctaggggccatctgcggagctggagttgtgaagggcttccagcagggtctgtacatgggcaatggcggtggtgccaatgtagttgccagtggctacaccaagggtgacggtcttggtgctgagattgttggcaccttcatcctggtctacaccgtcttctcagccactgatgccaagaggaatgccagggactcacatgttcctgtaagtatccttctcctttttgcagtgtgatatgtgaaagttcagtagtagttagtgtggtctaatgagttatgcactgtgataactaattcatcaccaaaccctaaagtgataatgtgcaaatcactaagcagtattttggttagcacctttacatacctcactcatacagtatgcctttaacaatgaactggtgtgctcataacttgtaggtgtgcgccatttatggctagacttttaactagtttaatttatcatgtttgctcaagaaattgactgtatgcgtatagttactggaagtatatatgggttttttgtgtgttgcataacgtatttctgtcctttgttggtgaaaacagatccttgccccactgccaattggttttgcggtgttcctggtccacctggccaccatccccatcaccggtactggcatcaacccagccaggagccttggcgctgccatcatctacaacaaggaccatgcctggaatgaccatgtgagcaacctttcttctctttttacctactcgccgttctcagcaattggttcctcttacattatcagatattagtcagcacatcattgataatcgatacattttttgttggcaaattgaggtctaagctgataaacatgtcagtatatataatcaaatgttctcagcaattggttcctcttacattatcagatattagtcagcacattactgataatcgatacattttttgttggcaaattgaggtctaagctgataaacatgtcagtatatataatcaaatgttctgatactttttttggttatatttttctctttgtatgggtaggtctaagatgataaacatgtcactataaccaaatgtagcagcatgctcttgctaaagtttacttagtagcttaccagcttactagcctttgctcaagaaaagagtagaagacacgacaaagagtttttccttacaaattttggtatctaaattttgcagtggatcttctgggttggtcccttcgttggcgctgccctggctgccatctaccaccaggtgatcatcagggcgatcccattcaagagcaggtcttaagccccgcgccgccgctgcgcagccgacgacatgcaacgcaatcgtgatgtcctgtttcccgcgcgctactgctgcgcatctgtcgattccctctatctctagtccccaagatgtttttcctatctgaaccctgaacaactcaatcgtgtaatccagtactcagtcactgtatgtttttatgtgatggagatcttaattcttaagttatcatctctgttgctgg</dnaseqindica> |
| External Link(s) |
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Liu C, Fukumoto T, Matsumoto T, Gena P, Frascaria D, Kaneko T, Katsuhara M, et al. (2013) Aquaporin OsPIP1;1 promotes rice salt resistance and seed germination. Plant Physiol Biochem.63:151-8.
- ↑ 2.0 2.1 2.2 2.3 Guo L, Wang ZY, Lin H, Cui WE, et al.(2006) Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family. Cell Res. 16(3):277-86.
- ↑ 3.0 3.1 3.2 3.3 Borstlap AC. (2002) Early diversification of plant aquaporins. Trends Plant Sci. 7(12):529-30.
