Difference between revisions of "Os01g0683550"
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development of the panicle. Someone suggest that the conserved function of the SUI-family is to specify stem organs in vegetative and reproductive development. Studies shows that loss-of-function mutations of the only known homolog of SUI in Arabidopsis display pleiotropic phenotypes which include severe dwarfism (Yamaoka et al. 2011), which indicates that SUI1-like genes play pivotal roles in stem development in both dicot and monocot plants. | development of the panicle. Someone suggest that the conserved function of the SUI-family is to specify stem organs in vegetative and reproductive development. Studies shows that loss-of-function mutations of the only known homolog of SUI in Arabidopsis display pleiotropic phenotypes which include severe dwarfism (Yamaoka et al. 2011), which indicates that SUI1-like genes play pivotal roles in stem development in both dicot and monocot plants. | ||
SUI-family genes encode BE-PSSs, which are crucial for PS maintenance in plants. SUI1 possess the enzymatic activity and can catalyze PC or PE into PS in yeast. Measurement of PS levels revealed that the total PS contents of neither the sui2 mutant nor the SUI1 ectopic expression plant was significantly reduced. The VLCFA-PS levels are significantly altered in both sui2 mutants and SUI1 over-expressed plants, implying that SUI-family genes might be directly involved in VLCFA-PS biosynthesis. It is possible that the distribution of VLCFA-PS in the membrane provides signal information for downstream function in SUI-family genes. Confocal analysis of SUI1:GFP reveals SUI1 protein localized in the plasma membrane. In Arabidopsis, the AtPSS1 protein was found mainly in nuclei and the ER membrane in pollen cells. Protein purification analysis in mammal cells indicates that PSS1 and PSS2 proteins are mainly localized in mitochondria-associated membranes. Taken these data, we speculate that the cellular localization of SUI-family genes is related to its function in diverse biological process, and the localization between SUI-family members could differ. | SUI-family genes encode BE-PSSs, which are crucial for PS maintenance in plants. SUI1 possess the enzymatic activity and can catalyze PC or PE into PS in yeast. Measurement of PS levels revealed that the total PS contents of neither the sui2 mutant nor the SUI1 ectopic expression plant was significantly reduced. The VLCFA-PS levels are significantly altered in both sui2 mutants and SUI1 over-expressed plants, implying that SUI-family genes might be directly involved in VLCFA-PS biosynthesis. It is possible that the distribution of VLCFA-PS in the membrane provides signal information for downstream function in SUI-family genes. Confocal analysis of SUI1:GFP reveals SUI1 protein localized in the plasma membrane. In Arabidopsis, the AtPSS1 protein was found mainly in nuclei and the ER membrane in pollen cells. Protein purification analysis in mammal cells indicates that PSS1 and PSS2 proteins are mainly localized in mitochondria-associated membranes. Taken these data, we speculate that the cellular localization of SUI-family genes is related to its function in diverse biological process, and the localization between SUI-family members could differ. | ||
| + | It is unclear how expression patterns of OSH15 and Histone4 are altered by SUI1 pathway. A previous study showed that ectopic expression of OSH15 could result in dramatic morphological change in rice (Sentoku et al. 2000; Nagasaki et al. 2001), and it is possible that a subtle change in the expression pattern of OSH15 might be responsible for the outgrowth of internodes in transgenic plants. Alteration of the cell division and differentiation patterns was evident from analysis of the expression pattern of Histone4. Notably, the function of the SUI1 pathway implicated a novel mechanism mediating lipid metabolism to gene expression. The SUI1 protein is localized to the plasma membrane and possibly affects membrane behavior. Gene chip analysis revealed that alteration of SUI-family genes could affect the expression of lipid metabolic pathway genes, which suggests that the SUI1 pathway is related to lipid metabolism. It is speculated that the SUI1 gene does not directly act to regulate expression of meristem functional genes, and instead that SUI-family genes are critical for generating and sustaining cellular microenvironments for the initiate and maintenance of the IM stem cell state. | ||
===Expression=== | ===Expression=== | ||
Revision as of 07:26, 7 June 2014
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Contents
Annotated Information
Function
The Shortened Uppermost Internode 1(SUI1) family of genes are pivotal for development of rice stems. SUI-family genes regulate the development of intercalary meristem(IM) for internode elongation and also the cell expansion of the panicle stem rachis in rice. The SUI-family genes encode base-exchange types of phosphatidylserine synthases (PSSs), which possess enzymatic activity in a yeast complementary assay.
SUI-family genes are essential for stem development in rice. . Stem development in rice includes both internode elongation and panicle rachis expansion. The elongation of internodes involves regulation of IM activity, which is controlled by SUI-family genes. SUI1 plays a prominent role in the specification of IM, which is coinciding with reproductive development. Experiment have showedthat sui2 mutations and transgenic plants with down-regulation of SUI1, SUI2, and SUI3 impaired the
development of the panicle. Someone suggest that the conserved function of the SUI-family is to specify stem organs in vegetative and reproductive development. Studies shows that loss-of-function mutations of the only known homolog of SUI in Arabidopsis display pleiotropic phenotypes which include severe dwarfism (Yamaoka et al. 2011), which indicates that SUI1-like genes play pivotal roles in stem development in both dicot and monocot plants.
SUI-family genes encode BE-PSSs, which are crucial for PS maintenance in plants. SUI1 possess the enzymatic activity and can catalyze PC or PE into PS in yeast. Measurement of PS levels revealed that the total PS contents of neither the sui2 mutant nor the SUI1 ectopic expression plant was significantly reduced. The VLCFA-PS levels are significantly altered in both sui2 mutants and SUI1 over-expressed plants, implying that SUI-family genes might be directly involved in VLCFA-PS biosynthesis. It is possible that the distribution of VLCFA-PS in the membrane provides signal information for downstream function in SUI-family genes. Confocal analysis of SUI1:GFP reveals SUI1 protein localized in the plasma membrane. In Arabidopsis, the AtPSS1 protein was found mainly in nuclei and the ER membrane in pollen cells. Protein purification analysis in mammal cells indicates that PSS1 and PSS2 proteins are mainly localized in mitochondria-associated membranes. Taken these data, we speculate that the cellular localization of SUI-family genes is related to its function in diverse biological process, and the localization between SUI-family members could differ. It is unclear how expression patterns of OSH15 and Histone4 are altered by SUI1 pathway. A previous study showed that ectopic expression of OSH15 could result in dramatic morphological change in rice (Sentoku et al. 2000; Nagasaki et al. 2001), and it is possible that a subtle change in the expression pattern of OSH15 might be responsible for the outgrowth of internodes in transgenic plants. Alteration of the cell division and differentiation patterns was evident from analysis of the expression pattern of Histone4. Notably, the function of the SUI1 pathway implicated a novel mechanism mediating lipid metabolism to gene expression. The SUI1 protein is localized to the plasma membrane and possibly affects membrane behavior. Gene chip analysis revealed that alteration of SUI-family genes could affect the expression of lipid metabolic pathway genes, which suggests that the SUI1 pathway is related to lipid metabolism. It is speculated that the SUI1 gene does not directly act to regulate expression of meristem functional genes, and instead that SUI-family genes are critical for generating and sustaining cellular microenvironments for the initiate and maintenance of the IM stem cell state.
Expression
SUI1 is highly expressed in the young panicle, internode, and roots but is rarely expressed in leaves. SUI3 is expressed mainly in the panicle and internode, and SUI2is expressed ubiquitously in all organs The SUI-family genes encoded base-exchange types of phosphatidylserine synthases, overexpression of SUI1 and SUI2 can cause outgrowths of internodes during vegetative development. Overexpression and RNA knockdown of SUI-family genes can affecte downstream gene expression related to phospholipid metabolic pathways.Also, the quantity of very long chain fatty acids Phosphatidylserine is affected by transgene of SUI-family genes.
Labs working on this gene
College of Agronomy and Biotechnology of Southwestern University
References
1.Hengfu Yin;Peng Gao;Chengwu Liu;Jun Yang;Zhongchi Liu;Da Luo
SUI-family genes encode phosphatidylserine synthases and regulate stem development in rice Planta, 2013, 237(1): 15-27
2.Yamaoka Y, Yu YB, Mizoi J, Fujiki Y, Saito K, Nishijima M, Lee Y,Nishida I
PHOSPHATIDYLSERINE SYNTHASE1 is required for microspore development in Arabidopsis thaliana,
Plant J, 2011, 67:648–661
Structured Information
ORIGIN
1 agcagagacc gccgtcgctt ccacagcatt cggctcccat ccatcaccgt agacgagtaa
61 tcaactccat tgatgccagc ggtgaggtga tggaatatgg atctagtaac gatcagagga
121 tgcaggacat ggaaatatgg ccgtcggacg gcggcggcgt ggaggaatac gacccgtgga
181 cggcttggct ttacaagcca cacacggtct cggttctcct tgccggcgca tgcctcctga
241 tctgggcaag tggggtcctt catccagaga ttacaagctc tcatgataag gtcataccta
301 ttaaaagggg tgtctgggct atgatcgcag ttttcctagc atactgcact ctccaagcac
361 cttcaacgat acttattagg cctcatcctg ctgtgtggcg tttggtgcat ggcatggctg
421 ttgtgtacct tgttgctcta acttttcttc ttttccagaa acgtgatgat gccaggcagt
481 ttatgaagca ccttcaccct ggtctcggag ttgaactacc agagagatca tatggttctg
541 actgccgtat gtatgttcca gaaaacccta ccaacagatt tattaatatt caagagacat
601 tgtttgatga atttgttatt gcccatgtct tgggttggtg gggtaaggca gtaatgatac
661 ggaaccaact tctcctatgg gtcttgtcag ttggttttga gctcatggag cttacattta
721 ggcacatgct gccaaatttt aacgagtgtt ggtgggacag tattatattg gatatcatga
781 tatgtaattg gtttggtgta tttgggcggg gatgcacaca gtccgttact ttgatggcaa
841 aacatacgaa tgggttggac tgggtaaaaa ggtcattatg tcagttcaca cccgctaaat
901 gggataagga tcaatggcat cctttcatgg agccaaggag attcatccaa gtgttctgtc
961 tctgcgtcgg tttcatgaca gtggaactta acaccttctt cctgaaattt tgtctctgga
1021 ttcctcccag gaaccccttg aaaaccagtg aagaaggtgg gagctttttg ttggctttcg
1081 ttagctatat gcatggtaga gctgcttata tgcatgaagt ttggacacgg tgagttattt
1141 tatagtatgt agcatattct tggcaaatta acaagggcat acagtttatg tcgatacatg
1201 cttggtcagt ctttgtgcta acatgaatca acataaccag gtttgtttca agatccaatg
1261 cctacttggt tgattactct ttggatttgt gtggggattt ctcttgcgct tttcttgctg
1321 gaatggtcac gaagg
