AB013448

Annotated Information

Function

The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.

Expression

The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.

Evolution

Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes.the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.

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Labs working on this gene

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References

1. Y. Koide;A. Kawasaki;M. J. Telebanco-Yanoria;A. Hairmansis;N. T. M. Nguyet;J. Bigirimana;D. Fujita;N. Kobayashi;Y. Fukuta. Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.),Plant Breeding, 2010, 129(6): 670-675

2. 刘洋;徐培洲;张红宇;徐建第;吴发强;吴先军.水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用.中国农业科学, 2008, 41(1): 9-14

3. Zi-Xuan Wang;Masahiro Yano;Utako Yamanouchi;Masao Iwamoto;Lisa Monna;Hiroshi Hayasaka;Yuichi Katayose and Takuji Sasaki.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal, 1999, 19(1): 55-64

4. L. Monna;A. Miyao;H. S. Zhong;M. Yano;M. Iwamoto;Y. Umehara;N. Kurata;H. Hayasaka;T. Sasaki.Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b,Theoretical and Applied Genetics, 1997, 94(2): 170-176

5. Masaru Miyamoto;Ikuo Ando;Krystyna Rybka;Osamu Kodama;Shinji Kawasaki.High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes. I. Pi-b.Molecular Plant-Microbe Interactions, 1996, 9(1): 6-13