Difference between revisions of "Os01g0217900"
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===Expression=== | ===Expression=== | ||
| − | ROS1a is the longest gene of six putative DNA demethylase genes（ROS1a–d and DML3a and DML3b） in the rice genome , comprising 17 exons that encode a protein with 1952 amino acids, and 5’ and 3’ UTRs of 73 and 607 bp, respectively.(figure1) ROS1a was toxic to a E. colidcm+ strain containing 5-meC in an ITG-dependent manner. | + | ROS1a is the longest gene of six putative DNA demethylase genes（ROS1a–d and DML3a and DML3b） in the rice genome , comprising 17 exons that encode a protein with 1952 amino acids, and 5’ and 3’ UTRs of 73 and 607 bp, respectively.(figure1) ROS1a contains an endonuclease III domain with significant similarities to base excision DNA repair pro-teins in the HhH-GPD superfamily. ROS1a was toxic to a E. colidcm+ strain containing 5-meC in an ITG-dependent manner. |
[[File:Schematic representation of ROS1a.jpg]] | [[File:Schematic representation of ROS1a.jpg]] | ||
Revision as of 15:16, 7 June 2014
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Contents
Annotated Information
Function
Gene Ros1a encodes a DNA demethylase, Repressor of silence 1a(Ros1a), which mediates 5-methylcytosine(5-meC) DNA demethylation in rice. It is a bi-functional DNA glycosylases that not only recognize and remove 5-meC from double-stranded DNA, but also has lyase activity, which nicks double-stranded DNA at an abasic site. It plays animportant role in both male and female gametophytes development, fertilization and endosperm development.
Expression
ROS1a is the longest gene of six putative DNA demethylase genes（ROS1a–d and DML3a and DML3b） in the rice genome , comprising 17 exons that encode a protein with 1952 amino acids, and 5’ and 3’ UTRs of 73 and 607 bp, respectively.(figure1) ROS1a contains an endonuclease III domain with significant similarities to base excision DNA repair pro-teins in the HhH-GPD superfamily. ROS1a was toxic to a E. colidcm+ strain containing 5-meC in an ITG-dependent manner.
Evolution
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Labs working on this gene
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References
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Structured Information
| Gene Name |
Os01g0217900 |
|---|---|
| Description |
Conserved hypothetical protein |
| Version |
NM_001185305.1 GI:297719744 GeneID:9271145 |
| Length |
3118 bp |
| Definition |
Oryza sativa Japonica Group Os01g0217900, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 1:6451851..6454968 |
| Sequence Coding Region |
6451852..6451888,6453241..6453279,6454305..6454441 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008394:6451851..6454968 source=RiceChromosome01 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008394:6451851..6454968 source=RiceChromosome01 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>gtttactttggaacttcaattccgacaatatttaaaggtttgacaactgaagaaatacaacattgcttttggagaggatttgtgtgcgtgagaggctttgataggacatcaagagcacccagaccactgtatgcaagactccactttccagcaagcaaaattaccaggaataaaaaatctgcaggttctgctccaggaagagatgatgaatag</cdnaseq> |
| Protein Sequence |
<aaseq>VYFGTSIPTIFKGLTTEEIQHCFWRGFVCVRGFDRTSRAPRPLY ARLHFPASKITRNKKSAGSAPGRDDE</aaseq> |
| Gene Sequence |
<dnaseqindica>2..38#1391..1429#2455..2591#tgtttactttggaacttcaattccgacaatatttaaaggtatttcactaataaattttgaccaagaataggatttttggcagcgccaaatgtgccactatctttattgtgtgaagtccattatgtgattgtaataatttgaatcaccaagaggactaaggcctgctttgggacatattacgagcagcttttgcttgcaaagaaaccagattctggtgccgcaccttctccgctcttctgccacccaagtccgtccaatacccctcattgagcgcttggatcctaaccccatctgccatcatgcatcatcctgctaacaactgcttccaccattgcctgtttctgttgttgggaggcactcacgctgcttgctatagtttaggttttctttgtgtcctgatttagatggaatttccagctgctgtcttttacataactagctaaatgtccgcgctttgctatggataatagaaaatatattataatattgtcaaataaattaaatatgttttatacgaaatgtgttaacaatccttttgctatagggaatattgaccttaatttgattttatatgtggctatccatttagatttgtttgtttttctaataataataagttcaagggctaatgtacaaaattgacaatgggagtaggtggggtggcagattcactgccaccaccactaccttcttttaaaggggtatatagatttgcagcagtggttgcttgatctgtgatttgaaatgtcaagtacacgctcatgcatcagcaccatatgtctacgctcctgacccaacatgcaaccaatgcaattgagggttggctctgatacaattactaatgtcctatatccaaaacaactataggcctatgaccaaacataattaataacctcgcttgcgcttttgtcctcacttgctccatgtaaaagggttaacccgaggttactatgttaggaatagctgggtttatgaaacggttcaactctcaactcctcatatagcactaattcatgtattgctgtcagcagtgatttgagttccagatcatgctcataagataggaccaaattgtccttactatctactccctccgtcccaaaatataaggtatttccggtcaaaatatcttatattttgggatggagggagtactatactacggacccaccaccaaatagtgccgcagaagagagagagagagagagaagagggggtgggggtgggggtgtatgggtgaaataagaatagtgccaagtatttgccaacaaatgaggcggtcaaatgtgtcacatcaattgggaagtatgtcagatcaactgaaaatttgattgggaaattattattcatgcaacaaagctgtacaactgatcccatgtttctatcgcaggtttgacaactgaagaaatacaacattgcttttggagaggtaatcatttttttttgtatgtacgttttggtttccataacaaagagagatgaagtgtataggtactatgtttactgacaaggataataatagtagcaagtatataggcagaggagcatgtctctattctaccagtattattactcataataactagtatatccttttttttgccatttcagctgatagctactctccagtcaaaatatttgccatctctattgaacttttcattgtcttctgaatgtatcttactcttggatcattaatatttcattttgtcacgatatagtggtataggacaataaaatcatgggaagtatttattttcatcaccaatctactcatataattttcaaatgacaattataaatatcttaaaaatatattgttagttgtcctgtataaaataattgtcacaccctagtccacagcgacaagaatttgtgtctacaggctagagtgagtactctagaagtatcttcataggaatcggaataaaatgccaatgtgaatgaacaaggatatcaagtataccctcaaaatctctagagaggattgcgtaaatatgtaggtgtaattaaacaattgtttcatatggagggttttcttaaaggaggtacaagacttatcaatatgggtaaagtagtttttatccataggcattgttggcagaaagctgcttagggtagaatgctactccctccgtcccacaatataagagattttgagtttttgcttgcaacgtttgaccactcggcttattcaaaaatttttgaaattattatttattttatttgtgacttactttattattcacagtactttaagtacaacttttcgttttttatatttgcaaaaaaaattgtataagacgagtggtcaaacgttgtacgcaaaaactcaaaatcccttatattgtgggacggagggagtacttatggatgccttttttgtccaagatgtcagtaacattttctttcagggatgtggatttttacttcttttttccctaactttttcaggatttgtgtgcgtgagaggctttgataggacatcaagagcacccagaccactgtatgcaagactccactttccagcaagcaaaattaccaggaataaaaaatctgcaggttctgctccaggaagagatgatgaataggccatctggaaaaccagaaaggaaataaagaggaggtacatatgatctgccagaagatcactgacctgaaatggatcgctgaccaataagttgccgtaggcaattcaattatttctggccatatacatctgctgaaagttatgaactccagccactgacgaattcgtggtgctggtattcttcggcaacatgatccatcatacagattctatgcttggttgttgcaagcaattcttatgcggtgacagttgctgctgatagggagaaaaggcatgtccggcggctcagcggctctaactgtactttcatatgagtggaaccgattgttgtacatgtgaaaagtttgccattcaaaatggtcattcatgttgttaggtcattcatgtagtcgatgtcaaattaatcatcaattatttgatttgattcattcacaagtttaattggctctagatacttgtgagctgcaggccagcaatgtcataatgtatgtcgggaaaaggtagatataaatcgtcagcatttacctg</dnaseqindica> |
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