Difference between revisions of "Os03g0718600"
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Plant cytoplasmic male sterility (CMS) results from incompatibilities between the organellar and nuclear genomes and prevents self pollination, enabling hybrid crop breeding to increase yields.The Wild Abortive CMS (CMSWA) has been exploited in the majority of ‘threeline’ hybrid rice production since the 1970s, but the molecular basis of this trait remains unknown. | Plant cytoplasmic male sterility (CMS) results from incompatibilities between the organellar and nuclear genomes and prevents self pollination, enabling hybrid crop breeding to increase yields.The Wild Abortive CMS (CMSWA) has been exploited in the majority of ‘threeline’ hybrid rice production since the 1970s, but the molecular basis of this trait remains unknown. | ||
Plant mitochondrial genomic transformation is currently infeasible, but CMS gene function can be tested by nuclear transformation of candidate gene(s) fused with a mitochondrial transit signal (MTS)[1,2] | Plant mitochondrial genomic transformation is currently infeasible, but CMS gene function can be tested by nuclear transformation of candidate gene(s) fused with a mitochondrial transit signal (MTS)[1,2] | ||
| − | The suppression of the WA352-interacting gene O. sativa COX11(OsCOX11) can produce male sterility. | + | The suppression of the WA352-interacting gene O. sativa COX11(OsCOX11) can produce male sterility ( Figure1 ). |
[[File:Figure1-d.png|right|thumb|150px|"Figure1. OsCOX11 RNAi was driven by the rice RTStapetum-specific promoter"]] | [[File:Figure1-d.png|right|thumb|150px|"Figure1. OsCOX11 RNAi was driven by the rice RTStapetum-specific promoter"]] | ||
| − | COX11 proteins are conserved in eukaryotes and function in the assembly of cytochrome c(Cyt c) oxidase[3] | + | COX11 proteins are conserved in eukaryotes and function in the assembly of cytochrome c(Cyt c) oxidase[3] ( Figure2 ). |
[[File:Supplementary fig 8.png|right|thumb|200px|"Figure2. Multiple alignments of COX11 protein sequences of five plant species and yeast. Accession numbers of the protein sequences are given in parenthesis: OsCOX11 of O. sativa (ABF98570), ZmCOX11 of Zea mays (ACG32461), SbCOX11 of Sorghum bicolor"]] | [[File:Supplementary fig 8.png|right|thumb|200px|"Figure2. Multiple alignments of COX11 protein sequences of five plant species and yeast. Accession numbers of the protein sequences are given in parenthesis: OsCOX11 of O. sativa (ABF98570), ZmCOX11 of Zea mays (ACG32461), SbCOX11 of Sorghum bicolor"]] | ||
Revision as of 17:26, 7 June 2014
In CMSWA lines, WA352 accumulates preferentially in the anther tapetum, thereby inhibiting COX11 function in peroxide metabolism and triggering premature tapetal programmed cell death and consequent pollen abortion.
Contents
Annotated Information
Function
Plant cytoplasmic male sterility (CMS) results from incompatibilities between the organellar and nuclear genomes and prevents self pollination, enabling hybrid crop breeding to increase yields.The Wild Abortive CMS (CMSWA) has been exploited in the majority of ‘threeline’ hybrid rice production since the 1970s, but the molecular basis of this trait remains unknown. Plant mitochondrial genomic transformation is currently infeasible, but CMS gene function can be tested by nuclear transformation of candidate gene(s) fused with a mitochondrial transit signal (MTS)[1,2] The suppression of the WA352-interacting gene O. sativa COX11(OsCOX11) can produce male sterility ( Figure1 ).
COX11 proteins are conserved in eukaryotes and function in the assembly of cytochrome c(Cyt c) oxidase[3] ( Figure2 ).
Expression
Please input expression information here.
Evolution
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You can also add sub-section(s) at will.
Labs working on this gene
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References
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Structured Information
| Gene Name |
Os03g0718600 |
|---|---|
| Description |
Cytochrome c oxidase assembly protein CtaG/Cox11 family protein |
| Version |
NM_001057624.2 GI:297601593 GeneID:4333922 |
| Length |
2954 bp |
| Definition |
Oryza sativa Japonica Group Os03g0718600, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 3:29862461..29865414 |
| Sequence Coding Region |
29862661..29862762,29862860..29862940,29863453..29863557 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008396:29862461..29865414 source=RiceChromosome03 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008396:29862461..29865414 source=RiceChromosome03 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>gtgaaggttaaacctggtgaaagtgctcttgcattttatactgctgaaaatcgtagttcagctccaataactggtgtatccacatataacgtagctcctatgaaggctgcaatatatttcaataagatacaatgtttttgctttgaggagcaaacacttcttccaggggagcagattgacatgccggtgttcttctacatcgatcctgagtttgagacagaccccaaaatggaaggggtgaacaatattgtcctttcttacacattctttaaggtgaacgacagttaa</cdnaseq> |
| Protein Sequence |
<aaseq>VKVKPGESALAFYTAENRSSAPITGVSTYNVAPMKAAIYFNKIQ CFCFEEQTLLPGEQIDMPVFFYIDPEFETDPKMEGVNNIVLSYTFFKVNDS</aaseq> |
| Gene Sequence |
<dnaseqindica>2653..2754#2475..2555#1858..1962#gtagcgtcgaacaccgtacagcgaatcgcggcggcggaatcacacatcgccgcctctcgcacgcgccgccgcgatgccgccgccgccgccgccttcgttggccaggctccaccaacgcctctccctctcgctcctccggggacgctccccccccgcggcggccgacgccttcctccgccgcgggctcgcctcgtccgcctcctcctcctcttccgccgccgccgccgcggcggtggcggcggcggcggcaggccgggagaagagctcgaggaggacgctagcgtacctgctaggcgtggccgcggcgatggtcggcgcctcctacgccgccgtcccgctctaccgccgcttctgccaggccaccggctatggcggcaccgtccagcgccgcgaggtctggtcttcatttccctaacttgcctctatctttcttgttctcaatcgtaatatgctgaaattaacactgtgctgggacatcgattaagctgtgcttgattttgtggatagcgtctggttccattattggcaggttttagttttacaatctgctgcttggcactgtgatatgctgaaattgatgcgattcatcagctatgtggcctttgtcatgtggagaccgccgttccagttcttatagatgccatggacacgaacgtgttgttgtattgccatgtgtaaattatgagtctagaagattgtaatctattgatagatggcacttcctctcgccgcctctcgcctctcgcactctgcattctattaacaactatgaatatctgtgctggaatttgttaaaatgcccatcaaagggtgatcttacaaatgtgttgggagtggcatgaaatagtttttgattcatttttggtgcttgtatggataactggtctaagtagacattgctgaagcagggttattgttggactctatgaattctgtcaaaaaatagtaataaattttctgggtagtgaatcattcattattcattactggtgcattgaaaaagttctgataaatttggcatctttgtaactacctgattcaagtgagtttgaacctggagtattattccttaacaatgtttggctctctacagagtgtggaggagaagatctcacgacatgctcgagacggaacaacaacttcaaggtgactcttcctatgcccagggtccagttttttcactacttgtcgtttccagttccgaaaggcagtgccatggtggtttttaaaatatactttctatctcttgatagagccttgttgcaatgtgagctcaaactaggcctgtgttgccgatctgttgcctgttgtgtaaattttcctttactgttctcttacatgatcagatggctttttgccaatgtttatggttcagaagtaccttcatttcaaatagtgggatgacatgaaaccacatgtgatgatagatttagatgaagtttaacttggaatagccatacagccttgccagcaattgcatgtgtacagcaaatttgctgtaatcatgggctaatattttggaaaaccattatcttcatgtttatatgattgcaactaattacaagagtttcgggataaatttttaaatgaatttgttgtttcttgtttatccaaataatctcgtgtgctaaaatttagctgtgattcagttttttgatgacactttattttctttgctgttgagactaatgcaattaattttatttgcagagagataattgtccaatttaatgctgacgttgctgatggaatgccgtggaaattcattccaacacagagagaagtaaatgctttgcttaagaaacaatttatagccctttccgcagcatgtttatcctctacctattccatttgtaggtgaaggttaaacctggtgaaagtgctcttgcattttatactgctgaaaatcgtagttcagctccaataactggtgtatccacatataacgtagctcctatgaaggtatgtaattcagcacaacttttacacccttaaactgctgccatgtcctggaaagaaagatttgtgagagatgcagaagtgtctctggattcacaaagctactgtaggcctttaactcttaatcctttgttaattttgttagcctaaattagtggaaatatgttggatgtaatcctttaacttttaatcctatattcttttttgttaaatatgttaagttaaatagccccaactctcaagcatgatttctaggaagaaaatttttgaaaccattagccatgattagacttcctcaactgataagagtttgaaaactgtgatcagaaaattgtagaaaacataacctctgctgtgatattcttagtcaaggagtgactcaggtgacttcacaatatcatcctatttatgttgctaactcaaattatgctgaggtgtacagctagggcatttaagctaacaggaggcagactcctaatgttttgagtttacaactcttttctttcatcctgtaggctgcaatatatttcaataagatacaatgtttttgctttgaggagcaaacacttcttccaggggagcagattgacatgccggtaaattcaacttgcattcgatgaaatgcatggctagttagttatctggtgtttgtctacacttattccaactttgtatgtgaacttttttcttcaggtgttcttctacatcgatcctgagtttgagacagaccccaaaatggaaggggtgaacaatattgtcctttcttacacattctttaaggtgaacgacagttaattgtatcggtaccgtaaagtaagtggtaacattagttctatttaaacaccttgccaacgagtaacacaaaaattcctttcatgtaacacaagttagggttttgtttagtgtgtattaattgacatgtgtttcttggaaattttgcaaataaatatgtaagaattgttcacagaacaatcgaaatggtgcatcttgatttg</dnaseqindica> |
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