Difference between revisions of "Os04g0622600"
(→Structure) |
(→Expression) |
||
| Line 5: | Line 5: | ||
===Expression=== | ===Expression=== | ||
| − | Xa1 gene expression was induced on inoculation with a bacterial pathogen and wound, unlike other isolated resistance genes in plants, which show constitutive expression. The induced expression may be involved in enhancement of resistance against the pathogen.<ref name="ref2" /> | + | Xa1 gene expression was induced on inoculation with a bacterial pathogen and wound, unlike other isolated resistance genes in plants, which show constitutive expression. The induced expression may be involved in enhancement of resistance against the pathogen.<ref name="ref2" />[[File:rice4.jpg|right|thumb|150px|]] |
===Evolution=== | ===Evolution=== | ||
Revision as of 03:26, 8 June 2014
Xa-1 is one of bacterial blight resistance genes in rice. Although at least 38 resistance genes have been identified, Xa-1 is specific to X. oryzae pv. Oryzae. Xa-1 can be considered as a dominant resistance gene[1].
Contents
Annotated Information
Function
The Xa1 gene in rice confers resistance to Japanese race 1 of Xanthomonas oryzae pv. oryzae, the causal pathogen of bacterial blight (BB). We isolated the Xa1 gene by a map-based cloning strategy. The deduced amino acid sequence of the Xa1 gene product contains nucleotide binding sites (NBS) and a new type of leucine-rich repeats (LRR); thus, Xa1 is a member of the NBS-LRR class of plant disease-resistance genes, but quite different from Xa21, another BB-resistance gene isolated from rice. [2]
Expression
Xa1 gene expression was induced on inoculation with a bacterial pathogen and wound, unlike other isolated resistance genes in plants, which show constitutive expression. The induced expression may be involved in enhancement of resistance against the pathogen.[2]
Evolution
Map-based cloning methods have been applied for isolation of Xa-1, one of the bacterial blight resistance genes in rice.Xa-1 was previously mapped on chromosome 4 using molecular markers. For positional cloning of Xa-1, a high-resolution genetic map was made for theXa-1 region using an F2population of 402 plants and additional molecular markers. Three restriction fragment length polymorphism (RFLP) markers, XNpb235, XNpb264 andC600 were found to be linked tightly to Xa-1, with no recombinants, and U08750 was mapped 1.5 cM from Xa-1. The screening of a yeast artificial chromosome (YAC) library using theseXa-1-linked RFLP markers resulted in the identification of ten contiguous YAC clones. Among these, one YAC clone, designated Y5212, with an insert of 340 kb, hybridized with all three tightly linked markers. This YAC was confirmed to possess the Xa-1 allele by mapping the Xa-1 gene between both end clones of this YAC (Y5212R and Y5212L).[1]
Based on the deduced amino acid sequence, the Xa1 gene is a class 1 resistance gene; a cytoplasmic receptor-like protein with NBS and LRR domains. Thus, the structure is quite different from the first cloned rice resistance gene, Xa21 (16), which is a class 4 gene. The most intriguing finding is that the expression of Xa1, unlike any previously studied resistance genes, is induced by pathogen infection and wound.[2]
Structure
- The composite nucleotide sequence of the Xa1 cDNA encoded a 5,406-bp ORF that was flanked by 59 and 39 untranslated regions of 112 and 392 bp, respectively. The nucleotide sequence of the 13.5-kb EcoRI-BamHI genomic fragment was also determined and compared with that of the cDNA sequence. The Xa1 gene was composed of four exons separated by three introns.[2]
- The C-terminal half of Xa1 gene is composed of LRR, the common motif of the several classes of resistance genes.
- XA1 protein contains 22 potential N-linked glycosylation sites. Six of these sites are at amino acid position 36 of each LRR repeat. These structural characteristics suggest that the Xa1 product interacts with other proteins in a defense reaction–signal transduction pathway[2].
Identification of the Xa1 Candidate cDNA Clone by High-Resolution Mapping and Sequence Analysis.We isolated a 340-kb YAC clone, Y5212, that spans the Xa1 locus (Fig. 1A) (27). To identify expressed sequences in the region of Y5212, a cDNA library of IR-BB1 (Xa1yXa1) was constructed with vector lambda gt10 and screened with Y5212clone DNA. Thirty-three positive clones were obtained; they were classified into 21 classes based on their 39 sequences.[2]
Labs working on this gene
- Department of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashiku, Fukuoka 812, Japan
- Rice Genome Research Program, National Institute of Agrobiological ResourcesySociety for Techno-innovation of Agriculture, Forestry and Fisheries, Kannondai, Tsukuba, Ibaraki 305, Japan
- National Institute of Agrobiological Resources, 1-2-1, Kannondai, Tsukuba, Ibaraki 305, Japan
- Plant Breeding Laboratory, Department of Agriculture, Kyushu University, Hakozaki, Higashiku, Fukuoka 812-81, Japan
References
- ↑ 1.0 1.1 Yoshimura S, Umehara Y, Kurata N, et al. Identification of a YAC clone carrying the Xa-1 allele, a bacterial blight resistance gene in rice[J]. Theoretical and Applied Genetics, 1996, 93(1-2): 117-122.
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 oshimura S, Yamanouchi U, Katayose Y, et al. Expression of Xa1, a bacterial blight-resistance gene in rice, is induced by bacterial inoculation[J]. Proceedings of the National Academy of Sciences, 1998, 95(4): 1663-1668.
Cite error: <ref> tag with name "ref3" defined in <references> is not used in prior text.
Structured Information
| Gene Name |
Os04g0622600 |
|---|---|
| Description |
Similar to XA1 |
| Version |
NM_001060453.1 GI:115460635 GeneID:4337041 |
| Length |
24820 bp |
| Definition |
Oryza sativa Japonica Group Os04g0622600, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 4:32036440..32061259 |
| Sequence Coding Region |
32036638..32036944,32037050..32037359,32038010..32040669,32041690..32042325,32059375..32060870 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008397:32036440..32061259 source=RiceChromosome04 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008397:32036440..32061259 source=RiceChromosome04 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggaggaggtggaagccggttggctggagggcgggatcaggtggctggcggagaccatcctggataacctggacgccgacaagctggatgaatggattcgccagattaggctcgccgctgacaccgagaagctacgggctgagatcgagaaggtggatggggtggtggctgccgtgaaggggagggcgatcgggaacaggtcgctggcccgatcgctcggccgtctcagggggttgctgtacgacgccgacgatgcggtcgacgagctcgactacttcaggctccagcagcaggtcgagggaggagttactacacggtttgaggctgaagagacggtcggagatggagcagaggacgaggacgatattccgatggacaatactgatgtaccggaggcagtggcggcaggcagcagcaagaaacggtccaaggcatgggaacactttactaccgtagagttcactgctgacgggaaggattctaaagcacggtgcaagtactgccacaaggacctatgttgcacatctaagaacgggacatcagctttgcgcaaccatctcaatgtttgcaagaggaaacgtgtaacaagtactgaccaaccggtaaatccatcaagtgccggtgagggtgcatcaaatgcaactggtaattcagttggcagaaaaaggatgagaatggatgggacttcaacacaccacgaggcagttagcacgcacccttggaacaaggctgaactttccaacaggatccaatgcatgactcatcagttagaagaggctgtaaatgaggttatgaggctatgtcgatcctcaagttcaaaccagagtcgacagggtacaccaccggccacaaatgcaacaacatcgtcttatcttccggagcccatagtgtatgggagggctgcagagatggaaaccatcaaacagctgatcatgagcaatagatctaatggcataaccgtcctgccaattgtaggcaatggagggataggaaaaaccactttggcgcaactggtctgcaaagatctggtaattaaaagtcagtttaatgttaagatatgggtgtatgtatctgataaatttgatgtagttaagattacaaggcagattttggatcatgtctccaaccagagccacgaaggaataagcaaccttgatacgcttcagcaggatcttgaggaacaaatgaaatctaagaagttcctcattgtcttagatgatgtgtgggaaatccgtacagatgactggaaaaaactactggctcctttaagacctaatgatcaggtgaattcgtcacaggaagaggcaacaggtaatatgataattttgacaactcgtatacagagtattgccaaaagtcttggaacagtacaatcaattaagttagaagctctgaaagatgacgatatatggtcactatttaaagtgcatgcttttggtaatgataaacatgatagtagtccaggcttacaggttcttgggaagcaaattgctagcgagctaaaaggcaacccactggcagcaaaaactgtgggttcactattaggaacgaatcttaccatcgatcattgggatagcattataaagagtgaagaatggaaatccctgcaacaagcttatggcatcatgcaagcgctgaagttgtgctatgatcatctatccaaccccttacagcaatgcgtctcttattgttctcttttccccaagggttattctttcagcaaagcacaactaatacaaatatggattgctcaaggatttgtggaagaatccagtgagaagttggagcagaaaggatggaaatatctagctgagttggtaaattcgggtttccttcagcaagttgaaagcacacggttttcatcagaatattttgttgtgcacgatcttatgcatgatttagcgcaaaaggtttcacaaacagaatatgcaactatagatggctcagagtgcacagagttagccccaagtatacgccatttgtcaatagtaactgattctgcataccgcaaggagaaatatagaaacatatctcgtaatgaggtgtttgagaaaaggttgatgaaagttaagtcaaggagtaagttgaggtcactggtattaattgggcaatatgattctcatttttttaaatatttcaaagatgctttcaaggaagcacaacatctgcgactgctgcagatcactgcaacttatgctgattctgattcatttctctccagtttggtaaattctacacatctccggtatctgaaaattgtgactgaagaatccggcagaactttgccccgatctctaaggaagtattaccatcttcaagtactagatattggctatagatttggaattccccgtatatctaatgatataaataatcttctcagcctgcggcatcttgttgcatatgatgaagtgtgttcttccattgctaacattggtaaaatgacctcacttcaggaactaggcaattttattgttcagaataatttaagtggttttgaggtgacacaattgaaatccatgaacaagcttgtacaacttagtgtgtctcaacttgaaaatgttagaactcaggaggaggcatgtggggcaaaactgaaagacaaacaacacttagaaaagctacatttgtcctggaaggatgcatggaatggatatgacagtgacgaaagctatgaagatgaatacggcagtgatatgaatatagaaacagaaggggaggaactgtcagttggtgatgccaatggtgcccaaagcttacaacatcacagtaatataagctctgaacttgcttcaagtgaggtgctcgaaggtcttgaaccacatcacggcctcaagtatctacggatatctgggtataatggatctacctccccaacttggcttccttcttcacttacctgtctgcaaacacttcatctagaaaaatgtggaaaatggcaaatacttcctttagaaaggctagggttacttgtaaagctcgtgttgatcaaaatgaggaatgcaacagaactctcaatcccttcactggaggagcttgtgttaattgcattgccaagcttgaacacatgctcctgcacttccatcaggaacttgaactccagtttaaaggttctgaaaattaagaattgccctgtactgaaggtatttcccttgtttgagatttgccagaaatttgaaatcgagcggacgtcgtcatggttgccccatcttagcaagcttaccatctataattgtcctctttcctgtgtgcacagttctctgccaccttcatctatcagcggccatgaagaagatggaatgtgtatccttccgcaatcacttgaggaaatttacatctgcgagtactctcaagagaggctgcagctctgctttccaggaagcctcacccgcctgaaaaaactagtagtgttgggaaaccaaagtttaacatctctgcagctccattcctgcacagcactccaagagttgataattcaaagctgtgagtcgcttaattctctggaaggcttgcaatggctcggcaacctcaggttgctgcaggcacacagatgcctcagtggttatggagaaaatggaaggtgtatccttccacaatcacttgaggaactttacatcagagagtattctcaagaaacgctgcagccctgctttccagggaacctcaccagcttgaaaaaactagaagtacagggaagccaaaagttaatatctctgcagctgtattcctgcacagcactccaagagttgatgattgaaagttgtgtgtcgcttaattctctggaaggcctgcaatggctcgtcaacctcaggttgctgcgggcacacagatgcctcagtggttatggagaaaatggaaggtgtatccttccacaatcacttgagggactttacatcagagagtattctcaagaaacgctgcagctctgctttccaggaaacctcacccgcctgaaaacactagtagtgttgggaaaccaaagtttaacatctctgcagctccattcctgcacagcactccaagagttgataattcaaagatgtgaatcacttaattctctggaaggtttgcaattgctcggcaatctcagggggctgctggcacacagatgcctcagcggccatggagaagatggaaggtgtatccttccgcaatcacttgagaaactttacatctgggagtactctcaagagaggctgcagctctgctttccaggaaacctcacccgccagaaaatactaggagtgttgggaagccaaagtttaacatctctgcagctccattcctgcacagcactccaagagttgatgattcgaagctgtgaatcgcttaattctctggaaggcttgcaatggctcggcaacctcagggtgctgcgggcacacagatgcctcagtggttatggagaatatggaaggtgtacccttccgcaatcacttgaggaactttacatccatgagtattctcaagaaactctgcagccctgcttttcagggaacctcactctcctgagaaaattacaagtaaaggggaactcaaatttagtgtctctgcagctccattcttgcacatcactccaagagttgataattgaaagctgtaagtcaattaattcgctggaaggcttgcaatcgcttggcaacctcaggttgttgcgggcattcagatgcctcagtggttatggagaatatggaaggtgtatccttccgcaatcacttgaggaacttttcatcagtgagtattctctagaaactctgcagccctgcttcctgacgaatctcacctgcttaaaacaattagaggtatcaggcaccacaagtttaaaatctctagaactgcaatcatgcactgcactcgaacatttgaagattcaaggttgtgcgtcgcttgctacattggaggggttgcaattcctccacgccctcaggcatatggaagtattcagatgccctggcttgcctccatatttggggagttcgtcagagcagggctatgagctatgcccacgactggaaaggctcgacatcgatgacccctctatccttaccacgtcgttctgcaagcacctcacctccctccaacgcctagagcttaactatcgcggaagtgaagtggcaagactaacggatgagcaagagagagcgcttcagctcctattgtccctgcaagagctccggtttaagtcttgctacgatctcgtagatcttcctgcggggctccacagccttccctccctcaagaggttggagatctggtggtgcaggagcatcgcgaggctgccagagatgggcctcccaccttcgttggaagaactggttatcgtagattgcagtgacgagctagctcatcagtgcagaactctagcaagcaagctgaatgtcaaaattaatggggaatatgtgaactga</cdnaseq> |
| Protein Sequence |
<aaseq>MEEVEAGWLEGGIRWLAETILDNLDADKLDEWIRQIRLAADTEK LRAEIEKVDGVVAAVKGRAIGNRSLARSLGRLRGLLYDADDAVDELDYFRLQQQVEGG VTTRFEAEETVGDGAEDEDDIPMDNTDVPEAVAAGSSKKRSKAWEHFTTVEFTADGKD SKARCKYCHKDLCCTSKNGTSALRNHLNVCKRKRVTSTDQPVNPSSAGEGASNATGNS VGRKRMRMDGTSTHHEAVSTHPWNKAELSNRIQCMTHQLEEAVNEVMRLCRSSSSNQS RQGTPPATNATTSSYLPEPIVYGRAAEMETIKQLIMSNRSNGITVLPIVGNGGIGKTT LAQLVCKDLVIKSQFNVKIWVYVSDKFDVVKITRQILDHVSNQSHEGISNLDTLQQDL EEQMKSKKFLIVLDDVWEIRTDDWKKLLAPLRPNDQVNSSQEEATGNMIILTTRIQSI AKSLGTVQSIKLEALKDDDIWSLFKVHAFGNDKHDSSPGLQVLGKQIASELKGNPLAA KTVGSLLGTNLTIDHWDSIIKSEEWKSLQQAYGIMQALKLCYDHLSNPLQQCVSYCSL FPKGYSFSKAQLIQIWIAQGFVEESSEKLEQKGWKYLAELVNSGFLQQVESTRFSSEY FVVHDLMHDLAQKVSQTEYATIDGSECTELAPSIRHLSIVTDSAYRKEKYRNISRNEV FEKRLMKVKSRSKLRSLVLIGQYDSHFFKYFKDAFKEAQHLRLLQITATYADSDSFLS SLVNSTHLRYLKIVTEESGRTLPRSLRKYYHLQVLDIGYRFGIPRISNDINNLLSLRH LVAYDEVCSSIANIGKMTSLQELGNFIVQNNLSGFEVTQLKSMNKLVQLSVSQLENVR TQEEACGAKLKDKQHLEKLHLSWKDAWNGYDSDESYEDEYGSDMNIETEGEELSVGDA NGAQSLQHHSNISSELASSEVLEGLEPHHGLKYLRISGYNGSTSPTWLPSSLTCLQTL HLEKCGKWQILPLERLGLLVKLVLIKMRNATELSIPSLEELVLIALPSLNTCSCTSIR NLNSSLKVLKIKNCPVLKVFPLFEICQKFEIERTSSWLPHLSKLTIYNCPLSCVHSSL PPSSISGHEEDGMCILPQSLEEIYICEYSQERLQLCFPGSLTRLKKLVVLGNQSLTSL QLHSCTALQELIIQSCESLNSLEGLQWLGNLRLLQAHRCLSGYGENGRCILPQSLEEL YIREYSQETLQPCFPGNLTSLKKLEVQGSQKLISLQLYSCTALQELMIESCVSLNSLE GLQWLVNLRLLRAHRCLSGYGENGRCILPQSLEGLYIREYSQETLQLCFPGNLTRLKT LVVLGNQSLTSLQLHSCTALQELIIQRCESLNSLEGLQLLGNLRGLLAHRCLSGHGED GRCILPQSLEKLYIWEYSQERLQLCFPGNLTRQKILGVLGSQSLTSLQLHSCTALQEL MIRSCESLNSLEGLQWLGNLRVLRAHRCLSGYGEYGRCTLPQSLEELYIHEYSQETLQ PCFSGNLTLLRKLQVKGNSNLVSLQLHSCTSLQELIIESCKSINSLEGLQSLGNLRLL RAFRCLSGYGEYGRCILPQSLEELFISEYSLETLQPCFLTNLTCLKQLEVSGTTSLKS LELQSCTALEHLKIQGCASLATLEGLQFLHALRHMEVFRCPGLPPYLGSSSEQGYELC PRLERLDIDDPSILTTSFCKHLTSLQRLELNYRGSEVARLTDEQERALQLLLSLQELR FKSCYDLVDLPAGLHSLPSLKRLEIWWCRSIARLPEMGLPPSLEELVIVDCSDELAHQ CRTLASKLNVKINGEYVN</aaseq> |
| Gene Sequence |
<dnaseqindica>199..505#611..920#1571..4230#5251..5886#22936..24431#catcagacgattaatccacgacgaccaggagcgctgccccgagccacacgcccacacgcactggtgaatcttctcctttccacctagttttcactctgcagtctcctctcctagctactactctgccttcccatcctcgcttgttgcagccctcttgcacacgccattggccacagtccaaggactgctcctgttccgatggaggaggtggaagccggttggctggagggcgggatcaggtggctggcggagaccatcctggataacctggacgccgacaagctggatgaatggattcgccagattaggctcgccgctgacaccgagaagctacgggctgagatcgagaaggtggatggggtggtggctgccgtgaaggggagggcgatcgggaacaggtcgctggcccgatcgctcggccgtctcagggggttgctgtacgacgccgacgatgcggtcgacgagctcgactacttcaggctccagcagcaggtcgagggaggaggtactgtctttgcatatatccgtgccttttaattaagtttgcaagctgcgttgcctgcaacaatggcgtattggcgtcagtttccaatccatgcttgtgctacagttactacacggtttgaggctgaagagacggtcggagatggagcagaggacgaggacgatattccgatggacaatactgatgtaccggaggcagtggcggcaggcagcagcaagaaacggtccaaggcatgggaacactttactaccgtagagttcactgctgacgggaaggattctaaagcacggtgcaagtactgccacaaggacctatgttgcacatctaagaacgggacatcagctttgcgcaaccatctcaatgtttgcaagaggaaacgtgtaacaagtactgaccaaccggtaaatccatcaaggtaatgctaatggagttctgaatttagtgtaaatccgttgaagtgtaaatttggcccgttacatctgcttaagatctcattctgtctctaatcttctaatagccaactcatggtcattttttttcctaatatatagtaccggtgatggtgcaccaaatgtaattagatgcaaggaaacaaaagtgaacaattgtatatatcaaatataattatatctaaaacatgagtagtgtatcaaatccaattctttcaaaaatctactatgcaaaattgagtgacaaaatctgctgcctttttttttttacagaaagcaaccaattaatataagtcaaatataaaaacgctttgtagtctccaataaaatagctcattgtttcgtttatacttatgtttataaatttaaatttaaaacttaattttggagttgattttgtggttttcttttcatcctattttattttacaacatttgattttgaatagttaagaatgcgtatataaaaattttacccataagttattttttaaattgttaataaatcgtaaggataatcataagtataagtgaaatgattcgctcttcatctacttaagattgcgttatattgctgacctttctaatcgcctaaccacgatcacatgctcttccagtgccggtgagggtgcatcaaatgcaactggtaattcagttggcagaaaaaggatgagaatggatgggacttcaacacaccacgaggcagttagcacgcacccttggaacaaggctgaactttccaacaggatccaatgcatgactcatcagttagaagaggctgtaaatgaggttatgaggctatgtcgatcctcaagttcaaaccagagtcgacagggtacaccaccggccacaaatgcaacaacatcgtcttatcttccggagcccatagtgtatgggagggctgcagagatggaaaccatcaaacagctgatcatgagcaatagatctaatggcataaccgtcctgccaattgtaggcaatggagggataggaaaaaccactttggcgcaactggtctgcaaagatctggtaattaaaagtcagtttaatgttaagatatgggtgtatgtatctgataaatttgatgtagttaagattacaaggcagattttggatcatgtctccaaccagagccacgaaggaataagcaaccttgatacgcttcagcaggatcttgaggaacaaatgaaatctaagaagttcctcattgtcttagatgatgtgtgggaaatccgtacagatgactggaaaaaactactggctcctttaagacctaatgatcaggtgaattcgtcacaggaagaggcaacaggtaatatgataattttgacaactcgtatacagagtattgccaaaagtcttggaacagtacaatcaattaagttagaagctctgaaagatgacgatatatggtcactatttaaagtgcatgcttttggtaatgataaacatgatagtagtccaggcttacaggttcttgggaagcaaattgctagcgagctaaaaggcaacccactggcagcaaaaactgtgggttcactattaggaacgaatcttaccatcgatcattgggatagcattataaagagtgaagaatggaaatccctgcaacaagcttatggcatcatgcaagcgctgaagttgtgctatgatcatctatccaaccccttacagcaatgcgtctcttattgttctcttttccccaagggttattctttcagcaaagcacaactaatacaaatatggattgctcaaggatttgtggaagaatccagtgagaagttggagcagaaaggatggaaatatctagctgagttggtaaattcgggtttccttcagcaagttgaaagcacacggttttcatcagaatattttgttgtgcacgatcttatgcatgatttagcgcaaaaggtttcacaaacagaatatgcaactatagatggctcagagtgcacagagttagccccaagtatacgccatttgtcaatagtaactgattctgcataccgcaaggagaaatatagaaacatatctcgtaatgaggtgtttgagaaaaggttgatgaaagttaagtcaaggagtaagttgaggtcactggtattaattgggcaatatgattctcatttttttaaatatttcaaagatgctttcaaggaagcacaacatctgcgactgctgcagatcactgcaacttatgctgattctgattcatttctctccagtttggtaaattctacacatctccggtatctgaaaattgtgactgaagaatccggcagaactttgccccgatctctaaggaagtattaccatcttcaagtactagatattggctatagatttggaattccccgtatatctaatgatataaataatcttctcagcctgcggcatcttgttgcatatgatgaagtgtgttcttccattgctaacattggtaaaatgacctcacttcaggaactaggcaattttattgttcagaataatttaagtggttttgaggtgacacaattgaaatccatgaacaagcttgtacaacttagtgtgtctcaacttgaaaatgttagaactcaggaggaggcatgtggggcaaaactgaaagacaaacaacacttagaaaagctacatttgtcctggaaggatgcatggaatggatatgacagtgacgaaagctatgaagatgaatacggcagtgatatgaatatagaaacagaaggggaggaactgtcagttggtgatgccaatggtgcccaaagcttacaacatcacagtaatataagctctgaacttgcttcaagtgaggtgctcgaaggtcttgaaccacatcacggcctcaagtatctacggatatctgggtataatggatctacctccccaacttggcttccttcttcacttacctgtctgcaaacacttcatctagaaaaatgtggaaaatggcaaatacttcctttagaaaggctagggttacttgtaaagctcgtgttgatcaaaatgaggaatgcaacagaactctcaatcccttcactggaggagcttgtgttaattgcattgccaagcttgaacacatgctcctgcacttccatcaggaacttgaactccagtttaaaggttctgaaaattaagaattgccctgtactgaaggtatttcccttgtttgagatttgccagaaatttgaaatcgagcggacgtcgtcatggttgccccatcttagcaagcttaccatctataattgtcctctttcctgtgtgcacagttctctgccaccttcatctattgtttccaaattatcgatcggtaaagtttcaacacttccaacggtgagggggtcatctagtggaacattaataattggactgcaccccgatgaagttgatgatgatgatggtttggaggattctgatcagctgaaaacgttggatgacaaagtactattattccataacctgaggttcctaactagcttggcaatatatggttgtcgaaatcttgcgactatttcaattgaaagtttaaggcaactcgtttgtttgaagagtttggaattatacggctgcccaaaacttttctcttcagatgttccaccagagcttacatgtgaatatatgtcaggagcaaatcacagcgccctcccatctctcgaatgtctctatattgaggattgtggaataacggggaagtggctgtctctgatgttgcaacatgtgcaggccctacaggaactgagtttagaggactgccagcagataacaaggctatcgataggagaggaagaaaacagtcaaccaaatcttatgtcagctatggaggatccgtcattaggatatccagatcgagacgaacttctgcgccttccgttaaatctcatctcttctctgaaaaaggtatctattacatattgctatgatttaacattctacggcagcaaggtagatttcgctggatttacctcccttgaggagttagtgatttcacgatgccccaagctggtgtcgttcttggcgcataacgacggaaatgatgaacagtcgaatggaagatggctcctaccgctatcacttggaaaacttgagattaactatgttgattccctaaaaacgctgcagctctgctttccggggaacctcacccgcctgaaaaaactagtagtgttgggaaaccaaagtttaacatctctgcagctccattcctgcacagcactccaagagttgataattcgaagctgtgagtcgcttaattctctggaaggcttgcaattgctcggcaatctcaggttgctgtgtgcacacagatgcctcagcggccatgaagaagatggaatgtgtatccttccgcaatcacttgaggaaatttacatctgcgagtactctcaagagaggctgcagctctgctttccaggaagcctcacccgcctgaaaaaactagtagtgttgggaaaccaaagtttaacatctctgcagctccattcctgcacagcactccaagagttgataattcaaagctgtgagtcgcttaattctctggaaggcttgcaatggctcggcaacctcaggttgctgcaggcacacagatgcctcagtggttatggagaaaatggaaggtgtatccttccacaatcacttgaggaactttacatcagagagtattctcaagaaacgctgcagccctgctttccagggaacctcaccagcttgaaaaaactagaagtacagggaagccaaaagttaatatctctgcagctgtattcctgcacagcactccaagagttgatgattgaaagttgtgtgtcgcttaattctctggaaggcctgcaatggctcgtcaacctcaggttgctgcgggcacacagatgcctcagtggttatggagaaaatggaaggtgtatccttccacaatcacttgagggactttacatcagagagtattctcaagaaattctacagccctgcttccagacgaatctcacttgcttaaaaagattagaggtatcaggcactggaagtttcaaatctctggagttgcaatcatgcactgcactcgaacatttgaagattgaaggttgttcatcacttgccacattagagggcttgcgattcctccacaccctcaggcatttgaaagtacacagatgtcccagattgcctccatattttgagagtttgtcaggacagggctatgagctatgcccacgactggaaaggctcgagatcaattatccctcaatccttaccacgtcgttttgcaagaacctcacctctctacaatacctagagctttgcaatcacggattggaaatggaaagactaacggacgaggaagagagagcgcttcaactcctcacttccctgcaagagctccgatttaactgttgctacaatctcgtagatcttcccacagggctccacaaccttccctccctcaagaggttggagatctggaattgcgggagcatcgcgaggccgctggaaaagggtctcccaccttcgttggaagaactggctatcgtagattgcagtaatgagctagctcagcagtgcagattgctagcaagcaagcggaaggtcaaaattaatcagagatatgtgaattgattactcggtggctttttccacctgcccaactggcatgggctcgttcaggcgttcaagctgctgtaaattccattgccgcaatgacgaccttcagaaccgttacacaatacaaaggacatatgatggctagatcaactgtcacatacaaattctataattctatctactgaaaaggatgattgctgttcattttcgtgattacagaaggaactgtgtatatcgtgctatttttgcattcacattgtgttccaggttgtgctcggatcagccaatttcggtggttaattcactttgctgtgtcctctgtgcagtgtgcactcaagaattgttcgcacatgcaattcagttgagcatcgcactacgcaagtttttttttttttgttaacaaaggagtggttgacagcattgcaggttgctaaaacagtgtcaaaaatttgctaacaaaagaattctcttcagaaatagtatgaaaataaatgccacataagtaatctgagtaaaacagacagaatcactagagaagactaacgacaaactcttctttttctatttctgtggtgaaaaactcagcatataatctcatgtatgcattggaggtgcataccatttcgatcatgtatgcatttagctgaacattatcaagaagcaaatttattgcacgggcaaccataagctaaactaattctcaagcaatttaataatcacaaaacagtctgcaataccaagatatataaaacttttgccagcatggagcaacaacactgaaacactacagtcaagacagctcccagaaaagattatatgttgctacttcaattgccacagttacaatcacccataatgacacatcaaattacataagagtattaagagtatatgacagttgtaacaaaacaactgcattgagaactagaagaacagacagccacatgaaccatattcacttcttcagatcagaaagcctggtgaactaccagtctaccaattgctaccgtagtctccatttttcacttatatgtacataggacacctgcgctatcagaatagccacaaagccagttcactcgctacaaattaatcttgactaaatccaaacagataccaactcctcttaatcaacttacaaaacacctacacttttgttgtctctacaccaccaatcacacaaaaggaaattaatcatccacctactcgtatcctactgttagaatggaggccttacaccagagaatgggcattgccagattctgagacactgtcctttgagctccctgaatctgaaccagtaacaaaaatcaaataagacacaacccaaaaccttccgttcatcaagaagttggatgttcaaattataaagtgacagttcgatcgtaccactggatgatgaggacccactgctcgcggcctcagtatccttctcaatctccactgattggtaagttgctgttggcatttcatccccgacatctacacaggggcggacccagggctggtgccgggtatgcaccggcatacccagaaaattcggaaaagatttagtaggcagtatgcatcagtgggctgaaactaagcaaaagcccaagaagtaaaagtctagctgttgcacagcccaccagaagatgatgttcccctttggctagcactcatgcggtagggtttggagcaggcgttggcggcgctgcgacagcgtggcgcgctgtggcactgtatcattgatcggcagcctcatggcaagcacgacgcatggacggtttgagcgtcgtggcgctgcggccggacagcgagcaccggtggcagaggctgccggtcactgggctaggcgctgactaaatcacctggtccagttccactcattatctgtccatgtccaagtatactcaaacttcttcagttctttgtctctctgatttttttttcttaacagatggagacatagattgattgaaaattgaaaactataatgagctaacaagtgaaatcaatgagagtacaaacttcaaaaattgaatacatgtttttgtgatttgtacgctgagatcaagttgaatcaatcagattagaatacgaagaagaatatgggtattctttttctttctttagcaaagcaccattagattctgcttcatcaggccatgccctatgcttcacatttctaatggtgatgggaaaccttagaacagggagggaaagtaactgaacaaagtgagttgtgttattttgttcttcaattcaccctcaggatacaccaatactttcctaaaattaagactatatttgtgtcatcatgtgcatatagtgtttgatttgcctctcttgatagctagcatgggcaaacccaggacaaaaattctgggtccgccactgcatctacatactcatccaactgctcggcctgtgtagtgatcttgtcaggatccttgctctcctgtaaacaacaaaacacatctattatacaattaaaggaatagaaaaaggagcctccacgttcgctctcatggcctagaaattctcacattaatcgaagaaaaagaaaaacagagtccatatatagaaatacaatttagaaatagttgaaattcgaaattaaaaaataaggaatattagaagatgagactagagtccatatagaaatacactttagaaatagttgaaattcggaattaaaaaataaggaatattagaagaggagtatagagtccatatagaaatacaattaggaaataatagaaattcggaattaaaaataaggaatattagaagtagagtatagagtccatatagaaatacaattaagaaaaaaaatagaaattcggaattaaaaaataagaaatattagaagtagagtatagagtccatataggaatttaaaactaactaaaattcggaaaaaaaaaagaagccaccacgttcgctctcatgacctagaaattctcacattaatcggagaaaaagaaaaggcagagtctatatagaaatacaattcagaaatagctgaaattcagaattaaaaaaataaggaatattaaaagaggagactagagtctatatagaaatacaatttagaaatagctaaaattcggaattaaaaaataaggaatattagaagaggagactagagtccatatagaaatatgattcagaaatagctgaaattcggaattaaaaaataaggaatattagaacaggagactagagtccatatagaaatacaattaggaaataacaaaaattcggaattaaaaataaggaatattagaagtagagtatagagtccatatagaaatacaattaagaaataaccgaaattcggaattaaaaataagaaatattagaagtagagtatagagtccatatagaaatacaattaagaaaaaaaatagaaatttgaaattaaaaaataaggaatactataagtagagtatagagttcatattggaatttaaaaccaactaaaattcggaataaaaagtagcctccacgttcgctctcatggtctagaaattctcacattaatcagaaaaaaagaaaaagcagagtccatatagaaatacaatttagaaatagctgaaattcagaattaaaaaataaggaatattagaagaggagactagagtagttattatacattagtagttttgaaaagttattgcaaaatttaaaattatgttgtcattgtaatatattttaataatataatgagaaaatatatatgatattatataagagaaaatataatgatgctagccgcgtaatctacgcggaccaccatgctagttaaccattataagagctcatttcacacataagctaatagcaaccaagaacaaagcatgctgctcactattgccatcgtcacatcaccgttgtccaccatcgtagcggtactgccattcaccagcatatcactatcattagccacggaagcatcaatgacatcggcattttcaccattaacaatggcagcccgtcgactcttgttgagcgccttcttgaatttgttgacgaatcgatcaagctcccactgtgtctcgacatccatctcatcaatatccagctcgatctcacccccaaccagctcaggattgccattcctcttccgcacaatctgcaacacattatgcatcttctcctctggcaagctctccaacccaaccctcaacaagttcttctcctccagggtcatctccctcttgttcggctcccttgccttcgttttccgcattttcacattccctgcccttggtttcacctgcgccggagctttcgccggtggcaattccggtggcggcactggcatcggcggctcaaggatcttgagttcctgctcgaaccaagacaccgacgctttgtacatcttctcaaaggacgcgaggaggtcgccggcgaaggtgtggacctcgtgtccagcagggttgtaccgcagcgcgttgctgaacgtgagccggatgtcggcggcgaagtcgtcgtgcgaggggtaccttccggcggcgaggttcgccctcaccgtgccgagatccatggggcacttgatgacggcgtggtagtcttggagtccgaggcggtcgacctccacgggggcgttgaaccagatgctccgcttgtccttccgcagcttcgccaggatctggtcgcaccgcttccgcatcgccgccctgagcttcgccggcggcggggggaggccccggcgaggcgagccatgggcgcttcggcggcttctgctgttgctgaccccacgagccaatgcgggagatgaggcgcggaccaggccgaggcccaactcgcctgcgactccgcgaggcccatgagagttcgtttgggctatcagtccattacggacaagaagcccgagggcgcggcccacatccttccgtcttccccgcctttccttccctgcctcgtcgaccgcgttatcccgtctcaagtctcaactcccaacctgcgatgcgccgtagaaatttgatactcgtgtcactcgtcgtcttcctttgcttcactaatcactccaatgacgcgacacgcatctctctcaaaaagaaaaggattggtatgtaactgtctacaagtgatgaattcatcactcacgcagttcactgcacatcgtcccagtacgcgcactatcttggcagcaaaaagttgtaccagaatgatcttgcgttcttggcaggacatgatcgatcaaaataatctcaagcactttggctacacacaatgaacgaaattttgcagctcggcagctcaccaaggatgggcgcaactcaaccacgactgaaatcggtgctgacacttttagcagcatctttgtgattcagccgtggtttctccggcgcgtctccttctgttcagagaaaaccgaatagttcgggttcggattcagttcgaagcaagcatggcttaaaatcagttcatgcacacacagctgcccccttggcatttgtgtgcactgacacgatgccgtctgtcctacgcgggttggtagtatgatgaaagggtttggaaaccgatctaaaaaaccgttcggatcatcgggtttttgactagccatgaaagtgaaattgtccgaaaaggataaaagagcgaacgacgtcgtcggtccagcacaaagctcagcgctcaatcacgtcagagaaaccgatcacttcgggtccgggttcggttctggagagctaactaaggacttacggagtggtatgttcaactgttcagagtatttaacttcacggccaatacgttgtcgtccaccagagtgcatgtgagcgtcacgacgcatcggataatttgaccattcgtaggttgctgttggcgagcacggcgaaacccgagacgcgggttcggtttcggttaatccgtttcgcggtcgcgcgcggccacgggcacacggccatggcgccatgccgctgtctccccctcgctggctgcgacgcttgactatttttattcttcatctccatttgattgatttaatctctcgttgcagcagcaggcaacgtgcaatcggttcggttttgctactgttcgggttcccctggccctctcccccctataaatacacgcctccaaagtccaatccaatccgccatgaacactcggtgctcgagccatttccaattccaagcttacatcgaatcaccatccacgacaaagaagacgcatacgctggtgatggagctcatggcctccctcatcaacatcttggcgctgatctccgaggcatgccgcagcgcggagaagctgccggcggcgctgatcactggcggcgtcgtggaggccgcggcggcgatcttcgtcgccttcttcaagccgcccggtggcgtatttcagcaccacggcaaggcaccattctacctgtattacggcattataggaggcgtggccatcttcggcttcgcggaggcgtgggccgggttctgggtctccggcgacctgaacggccggcgcgccgtcggaaagacgatactgtgggtgtcgattttgcctcttgtcttggtagctgcgctcggagggttcgtcttcatgagatagttgcaaacctatgttaatttcctctgtttgtacgagtgggtgtaatctcctgtacctctgtgtgtagtagttgtagcgtgtatgcactagtttttgctttgtaatttttagcctggtgtatttatgatatttcagttattatgtgatgtaacagtatgattgttaacttttacaaggttttatgtaattatacataaggttattttttgtgtgatactccctccgttttttaatagtgacgccgttgactttttctcacatgtttaaccattcgtcttattcaaaaaatttatgtaattataatttattttgttatgagttattttatcactcatagtacttcaagtatgatttatatcatatacatttgcataaaatttttgaataagacgaatcatcaaacatgtgagaaaaagccaatggcgtcatatattaaaaaacggaggtagtatgtgattatgcgcgttttccttgcatttctttagctacggatgttgtacaggtgtacctcttttatgagtattcctccaatcttctgcttatatccttctcgaaatcatggcagtaatcaaccaatcataatcttaaaagaatcatggtagacgcagagcaaccagttagtacatatttgaaagaacaggcccttgcattgcagggccgtatcctgtaggctgcggtgtttcaaagcaggaaaaagtacaccgaaggtccctcaacttgttatcgagttacaaaatcgtccctcaaccgcaaaaccataccggacgtccctcaactaacaaaactgttcactttaacggtggttttgaccccggttttatttgaggtggcggctgagtcagcgtgggacccacgtgggccccacatgtcagaatgccaggtcgtctctctctcctcttctctcccttcctcgtctctccctctcacttctctcctctctgcagggcggctggcgggtggggaggaggtccggcagccagcggcggcgcgtgcgacctcggggtcggaccgtcggagaggcccgggaggcggctgcggcggcggcggcgacgcttgagaagggagaggcggccggtggcgccggcgcgggaagaagctgtcttcggcgtcgtgcccgctctgccctccaccgcctgcttcgcccgccgctgccccgcgctcacacactcccgccggcgacgccgctgacggcgcgggcgcgagaactgcggccatggatgacgacggcggcgacatcctcgctccccccgttccgctccccgtcggcgccatcttcctctcccgcagcgcgcctgcccgccgctcgtctgctccgtcggccggccgctccccgcgcagtcggcggtggcgaaggtgaccgtgccggcgtcgaggtcgtagccgacgtggatgttctgctgcgccaggttcccaaggatggacaccggctgctgcttcgtcgtcgccacgattgccaggcacagcgtcccctcctacaccgtcacggacgcgttctccggcttcagcgccaccgccgcgcggccgccgccaccgccgaactccagcgtcaagtccggactcgcttgttcggtttgcttcctgctggatgtacacatgaggtcattcaagatcagtaactcatactctattctagcaagctggctgttggtgagaattctgaattctcattgcatcgcctaacgaagatagaagatcggagtgttatacatcaatcaatttcttagctgggaatgttactcctgcattgctctacaatttatgggtcatgtaatttgatctctcacagaatctatagcgaggtaagaaagcgaaaccggcgccacaaaatatcttgaaattctgcttgttcatacactgttcttctgattaaagaacaacttagcttgagattgttctgttcacttgcaatttgcaggagtaacaaagcttatttttgcattgcccgagtccacaatgatgcgggagctcgccgccgatgccaccgtcttgttgccgaccctgacgaagtcgaggaccacggtgtagtacgtgtccacgtccccggcgacgagcggcgtgctcgctgcgccgagctcggtgacgttggcgagggtgccgaagttgaacgccgacgatgagttgacggagtgcgggacgaggctgtaggagaatctccggccgagcgacgcctcgccgccgagctgcgtcacgagggagaccgcgtcgccgctgagcccgaccagcccgtcggccgggaacgagccggccgtcgtggtggagcagccgaacttgacgctgcacacgccgcctcgtggtcgtgggcctcgtgggtggcggccggcgggggagcccgcgagccgctcctacccctgccgccctccaccactgcccgcccgcaggtcctccccccactgctgccgcgtcgcccgtcgccgccaccaccgcagccgcgtcgccatcgaatcggggagaagtcagaaagacatgaggaaaggagagaagaggggaggagagagatgacctggcatcctgatatgtggggcccacgtgggtcccacgctgactcaaccgtcacgtcacacaaaaccaggatcaaaaccaccggagaatctaaagtgaacggttttattagctgagggacgtccggtatctggttttgtggttaagggacgattttgtaactcgatgataagttgagggaccttcggtgtactttttcctttcaaagcataatgaagaaatcaaacttgtcaggtctcagatggctcgcaagcagttctccggaaaaggaggagagaagaagatatggacatacggggttaatactaatgcattagcataagctgttcagttccgggttcggttgtgacatgtactgatttctttgttaatagttaatcagcggaaatcatgtctttttcttattggtgggtccccgactgtggatctttttgcgcgcctctcttggtttggattgcaaagaagcctaccgacccggtttcgggttcgggttcgggttcgttgctcagcgcttgtgttgctttgctcaggcatactgcatacatatgctttcagaatgtctctatcaatccgggttgaaaaaagtggtcttgttgagatgaaattgcttatcggcaccattcgggttcggttcaagtcgtcggtttcctgtactactgctagtcagaaaaccgagctacgtggccgatcgggttcgggtttccgagagcaaacaacgtagcgtgtgctcgtcattttgcaatcgctccacttgctgcgagctcggcgaacggttaatctggacgccatccctacgaaaacccgagacgcggtctggcgggggtcggtgcttcggttcggttcatggtatagatggcctttcggcccgcccggcacggcccggcccaggaacggcccaccagccatcgggccggcacggcccgatcggcacttcgtgccgggccgtgccgacccatgggctgcacctcccgcccaggcacggcccaccagccgtcgggccgtgccgggccggcccgaaggcgcgggtggcccatcgtgcctttttatataagtctatattccatccctcctctttgggccgtgaaatatatatagcgaaaataagtctatttgccgtccctcttctttgggctgtggcatatatatagtgaaaataagtctattttctgtccctcctctttgggttgacatatatatatatatagctaaaataagactattttacgtctctattctttcgaccatggcatataatatgtctatttttactccctattgtttatgtgtcgggcctagcctgaggcccatcgtgccgtgccggcccggcacggccgggccggcccggtagtcgggccgggccagcacgggcccaacccctaacgggccgtgccgtgcttgggccgggccaaaacgccgtgccgtgggccgggccatcgagcgtcgggccttttggccatctatagttcatggtgtgtgggctgtgttccctatgggaccattcgggtgatttaattagcacgaaaaacggagtagttaattagcacgttgattaattaagtattatttaattttctttttaaaaatggatcaatataaattttttaaagaactttcatacagaaatttttttaaataaacatactgtttgtcagtttaaaaaacgtacacgcggaaaacgagaaaggagtgttaagaatttgctcttgcaaacacagccatggatggacacgatggatcatggatcgctgcgacttgcccgcgaatgaatctccgtcgagggctcgaggcagtcctgggtttggctttgtgcgtcgggtttcgccggtgtattaaacaggcacaagcataaaactccaagtcaccgagcagctccgatttttttatttttagtttttattaaaatatttacaaaactaattttgtgttttaaaaatttacaaaactagtcacccatcgcccgtggcagatggcagttgcctcatgcctaacgggcggcaaagaagggcgtgccattttgcagatggccccccttgctgcccttaggttataaataccaaagaggccgcttccaaaatgccaagccgtcctcgccgcccttttgcaggcggcccccgcacagtagaaaatcgccctctataagggtggcaagggggccgcctgcaaaaatgtccctacggacggcaggagggtagcctgcaaaatggcaggccttcctttgccggccgttagacatgaggcaacgatcgtctgccacgtcattaaaatcgatctttaggagggatttatggaccagtaagtccaacgtgtgcaaagtattccacccgaaaaatccgtcgggaaaccgatagagtatatgcgtggccgttcgggtttatcattttgtatttcttccgcttgcgcgcgttggcactgcactgctcactacgctctcggcgagtgattactctggtcgcgcgacaacccgtaacgcgatctcgcggtcgcgggttcggtttcggttgagctgtggtgtggatcgctgcaacttttgcttgaaaaatgaaaaacatgttcgtccgcgattttctgtccgtcgcctctcactgatcgagagatgcaacacagtctgagcgatcgggttcggtttttgtgctctgctttcaccatgaagtcatcaccaggctcactgcgtctgcctcctcgcaccattcagcattcaccgagttgaaaaaaccacaaactaaggcgttccccctgatgatacagcacgcgagctacgcacgcacagacatacggtacagacatgccggcgcccgcatcgacgtcgagaagctgctcactcatcccgtgccggcgtcttgcaagtcgcggcggcggcgctctgcctgggcttacggcccaaattcggcctgcaggcccgattttgagttgtcaagtcagagcccaccgacttggaggcagtcggcccaagcatttgaaagcccagaaacatctgctccgagtgctcaccttccgtgtagcttcctagtagttcctacactaccaaactaccgcattatttatatccctcacaaaaaaaaaataaaaaggactacccgcattttatattgctcatctgccctcctcgaatacctcccatattattcccatctctgccttttgcttaccactgaccactgtcttctcgcgaggctcgccactggtccattgtcgcccaagctccaaaggcttctcttccgccggatctgatggaggaggtggaggtcggtttgctggagggagggatcgggtggctggtgcagaccatcctggagaacctggacaccgataagctgggtgagtggattcgtcaggttgggctcaccgatgacaccgagaagctcaggtcagagatcgagagggtggaggtggtgacggctgccgtgaaggggagggcgatcgggaacaggtcgcttgcccgatcgctcagccgtctcagggagctgctctacgacgccgacgacgcgatcgacgagctcgactactacaggctccaacagcaggttcaaggaggtaaagcttgtgtatgcaagatgtatccatttttgtgtccaaggaggtgcattcattcccctttttctgttgtttcagatgcatggcaaggtggcactggaagtttagatgaacctgaagcagagcaagcagagagaccgagtatcaacgctgctattgcgattagcagtggtagcaaaaagcggtccaaggcatgggggcactttgatatcactgaagaagaaaatggaaagcctgtgaaggcaaggtgtattcactgtcacacggtggtcaagtgcggttctgaaaaagggacatcagttttgcataatcacctcaagagtggcagctgtaacaagaagcgtgaggcaactgatcagcagccaaacccgtcatcaaggtacggtatatgatttatgtaacgtactttcccccgcagcaagccagcaactgtgaaaccgttctgtttttttttaaaaaatatttttattttgcagtactgctgatactgcagcaaatagcactcttgttgaactcggcggttcaggttcagacatcagaaaaaagatgaggattaatggtgagtcaacacacaacgatgcaccttatgcacacccttggaaaaaggctgaatgttccacaaggatacagcaaataactcgtgagttacaagatgcacggggggctgtgagtgaaattcttaagctacatggaccgtgctctgttggaaattcaaaccatcgtacgagtacaaccacaactctctgcagaagaacgtcaagtcttaatccacacaaaatatatggaagagacgcagagaagaacaccatcatgaagattattacagatgacagttatgacggagtaactgtagtccctattgtgggcatcggaggagttgggaagacagctctcgctcaacttgtatacaacgaaccaacggtgaaacgtgactttgagcggatatgggtttgggtgtctgataactatgatgaattgaggatcacaatggagattctagattttgtctctcaagaaagacacgaagaatctccctgtagaaaagaaatacggaaaggagtaagtagctttgcaaagcttcaggagattttgaatgggtatatggacatccagtcgaaaaagtttttgcttgttttagatgacgtatgggacagcatggatgattacagatggaatattttgttggatccattgaaatcaaatcatccaaaaggtaatatgatccttgtgacaactagacttttgtctctcgcacagaggataggcacagtcaaaccaatcgagttaggtgctttgtcaaaagaggatttttggttgtattttaaaacatgtacatttggtgatgagaattacaaagcacatccaagtttgaacatcattgggcagaagatagctgacaagttaaagggcaatccattagcagcaaaagcaacagcgctgctattaagagaaaaacttactgttgatcattggagcaacattctgatgaacgaagattggaaatccctgcatttcagtagaggcatcatgcctgctttgaagcttagctatgatcagctgccttaccatttacaacagtgtttgttgtattgttccatattccctagtagttatcgctttgtcagcaaggagttgatctgtatttggatttctcaaggctttgtgcattgcaactcttcaagtaagagactggaggagatagggtgggactacctaactgatttggtgaactctggcttctttcagaaagttgatcatacacactatatcatgtgtggccttatgcatgattttgcaaggatggtttcaaggactgagtacgcaactatagataatctacagagcaacaaaatactgccaactatacgtcatttgtcaatactaaacaattctgcacactatgaagatcctagtaacgacaaggttgaaggaagaattagaaatgcagttaaagcaatgaaacatttgaggactttggtgctaattgggaaacatagctctttattcttccaatccttcaaagatgtagtccagaagggacatcatttacgtctgttgcaaatctctgaaacatgtacttatgttgaccccttgctttgcaatctggtgaatccagcccatattcgctatatgaagcttcacaaaagagctttgcctcaatctttcagcaagttttaccatcttcaagtattagatgttggctcaaaatctgatctgattatacctaatggtgtggatgatctagttagtctgcagcatcttgtagcagcagagaaagcatgctcgtccatcactagcatcagcaaaatgacctctcttcaggaactacataactttggtgttcaaaattctagcggctgggagatagcacaactccagtccatgaaccagcttgtacagctcggtgtgtctcaacttgaaaatgtcacaactagagctgaggcttgtggtgcaaaactaagagacaaacagaacttagaaaagctgcgcctttcgtggactaatttacataaattgggtcatttggggactaacgtgccatgggatgaacgtgaaaatgcaagagcagtgcttgagggtcttgaaccacatacaaatcttaagcacctagagatatattcgtacaatggtgctacccctccaacatggcttgccacttcacttacctctttacagactctccgtctagagtgttgtggacaatggaaaatgattccatcactggaacgtcttccctttcttaaaaagatgaagttggagagtatgcagaaaataatagaaatgacagttccttcgctggaggagctgatgttaattgacatgccaaatttggagagatgctcctgcacttccatgagggacttaaactgcagtttaagggttctgaaggttaaaaagtgccctgtgctgaaggtctttccattgttcgaggactgccaaaaatttgaaattgagcggaaatcatggttgtcccaccttagcaagcttactatccatgattgtcctcatttgcatgtgcacaatcctcttccaccttctactattgttttggaattatccatcgccaaagtttcaacacttccaacgttgaaggggtcatccaatggaacattaacaatttggcttcccaatgatgatgatgttcctgataagctgataacgttggatgataacattatgtcgttccataacctgagtttcctaactggattggaaatatatggtttccaaaatccgacgtctatttcattccatggtttgaggcaactcagatgtttgaagactttaaaaatatacgactgcccaaaacttctcccttcaaatgttccatcagagcttaccggtgaatatatgtcaggagaaaatcacagcgcccttccatctctcgtacgtctccatattgagaagtgtggaataatgaggaagtggctgtctctgttgttgcaacatgtgcaggccctacaggaactgagtttagataactgcaagcagataacagggctatcgttaggacaggaagaaaacaatcaaccaaatcttatgtcagctatggaggatccatcattaggatatccaggtgaagataaacttatgcgccttccattaaatctcctctcctctctgaaaaaggtatctattacattgtgcaatgatataacattctacggcagcaaggaagatttcgctggatttacctcccttgaggagttagtgatttcacgatgcctcaagctggtgtcgttcttggcgcataacgacggaaatgatgaacagtcgaatggaagatggctcctaccgctatcacttggaaaacttgagattaaacatgttgattccctaaaaacgctgcagctctgctttccaggaaacctcacccgcctgaaaacactagtagtgttgggaaaccaaagtttaacatctctgcagctccattcctgcacagcactccaagagttgataattcaaagatgtgaatcacttaattctctggaaggtttgcaattgctcggcaatctcagggggctgctggcacacagatgcctcagcggccatggagaagatggaaggtgtatccttccgcaatcacttgagaaactttacatctgggagtactctcaagagaggctgcagctctgctttccaggaaacctcacccgccagaaaatactaggagtgttgggaagccaaagtttaacatctctgcagctccattcctgcacagcactccaagagttgatgattcgaagctgtgaatcgcttaattctctggaaggcttgcaatggctcggcaacctcagggtgctgcgggcacacagatgcctcagtggttatggagaatatggaaggtgtacccttccgcaatcacttgaggaactttacatccatgagtattctcaagaaactctgcagccctgcttttcagggaacctcactctcctgagaaaattacaagtaaaggggaactcaaatttagtgtctctgcagctccattcttgcacatcactccaagagttgataattgaaagctgtaagtcaattaattcgctggaaggcttgcaatcgcttggcaacctcaggttgttgcgggcattcagatgcctcagtggttatggagaatatggaaggtgtatccttccgcaatcacttgaggaacttttcatcagtgagtattctctagaaactctgcagccctgcttcctgacgaatctcacctgcttaaaacaattagaggtatcaggcaccacaagtttaaaatctctagaactgcaatcatgcactgcactcgaacatttgaagattcaaggttgtgcgtcgcttgctacattggaggggttgcaattcctccacgccctcaggcatatggaagtattcagatgccctggcttgcctccatatttggggagttcgtcagagcagggctatgagctatgcccacgactggaaaggctcgacatcgatgacccctctatccttaccacgtcgttctgcaagcacctcacctccctccaacgcctagagcttaactatcgcggaagtgaagtggcaagactaacggatgagcaagagagagcgcttcagctcctattgtccctgcaagagctccggtttaagtcttgctacgatctcgtagatcttcctgcggggctccacagccttccctccctcaagaggttggagatctggtggtgcaggagcatcgcgaggctgccagagatgggcctcccaccttcgttggaagaactggttatcgtagattgcagtgacgagctagctcatcagtgcagaactctagcaagcaagctgaatgtcaaaattaatggggaatatgtgaactgattactcggtggcttgttaggcgcacctttttccacctgcccaactggcgtgggctcgttcaggcgttcaagctgctgtaaattccattgccgcaatgacgaccttcagaaccgttacacaatacaaaggacatatgatggctagatcaactgtcgcagagctagctttggttcacctgaaaacataaggccaaacgcgtggttcttttaatcagaagtatcaaaaattggttttggtttttaacgaaaagatgttagaaattggttttaatgtgccagattctgtaccagaattctgtattttgctccgtaattctgtgctacgactgatgttgtatttaactgatagcaatcgaccggcaaaaccaatccgctccaatgcattgtcac</dnaseqindica> |
| External Link(s) |
