Difference between revisions of "Os04g0677500"

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stems, and cortical parenchyma cells in roots<ref name="ref2" />. the Representative sequence Os04g0677500 is AK100393[[File:File0.jpg]]
 
stems, and cortical parenchyma cells in roots<ref name="ref2" />. the Representative sequence Os04g0677500 is AK100393[[File:File0.jpg]]
 
this gene expressed in different Organs and tissues of rice , and their Feature Number is 9888 21058 422901. their expression as following pictures show.[[File:File1.jpg]][[File:File.png]][[File:File3.png]][[File:File4.png]][[File:File5.png]][[File:File6.png]][[File:File7.png]].
 
this gene expressed in different Organs and tissues of rice , and their Feature Number is 9888 21058 422901. their expression as following pictures show.[[File:File1.jpg]][[File:File.png]][[File:File3.png]][[File:File4.png]][[File:File5.png]][[File:File6.png]][[File:File7.png]].
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Quantitative RT-PCR and GUS staining analyses exhibited that OsPK was expressed mainly in leaf mesophyll cells, phloem companion cells in
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stems, and cortical parenchyma cells in roots. The transcriptions of four other putative enzymes involved in the glycolysis/gluconeogenesis pathway were altered in ospk<ref name="ref3" />.
  
 
===Evolution===
 
===Evolution===

Revision as of 10:22, 8 June 2014

Please input one-sentence summary here.

Annotated Information

Function

this gene named Os04g0677500 is located in rice CHR 4:35005101-35008954,which the length of this gene is 3854bp.the expression of this gene is Rice mitochondrial Pyruvate kinase [1]. Pyruvate kinase (PK; ATP:pyruvate 2-O-phosphotranferase, EC 2.7.1.40) is a key regulatory enzyme of the glycolyticpathway. It catalyzes the final step of glycolysis, converting ADP and phosphoenolpyruvate (PEP) toATP and pyruvate by essentially irreversible transphosphorylation.This enzyme is critical for the control of the metabolicflux through the glycolytic pathway. Moreover, its substrate (PEP) and product (pyruvate) are both involved in a variety of metabolic processes. Thus, PK plays a key role in the cellular metabolic flux [2].Pyruvate kinase (PK) catalyzes the final step of glycolysis.Pyruvate kinase (PK; ATP:pyruvate 2-O-phosphotranferase,EC 2.7.1.40) is a key regulatory enzyme of the glycolytic pathway. It catalyzes the final step of glycolysis, converting ADP and phosphoenolpyruvate (PEP) toATP and pyruvate by essentially irreversible transphosphorylation.[3].

Expression

using bioinformatics methods to scan rice genome, we get 10 with PK extremely conservative regions (PK - PK - C) gene.Os04g0677500 is one of that Homologous genes ,and it was highly expressed and composed of sexual expression [1].OsPK1 was expressed mainly in leaf mesophyll cells, phloem companion cells in stems, and cortical parenchyma cells in roots[2]. the Representative sequence Os04g0677500 is AK100393File0.jpg this gene expressed in different Organs and tissues of rice , and their Feature Number is 9888 21058 422901. their expression as following pictures show.File1.jpgFile.pngFile3.pngFile4.pngFile5.pngFile6.pngFile7.png. Quantitative RT-PCR and GUS staining analyses exhibited that OsPK was expressed mainly in leaf mesophyll cells, phloem companion cells in stems, and cortical parenchyma cells in roots. The transcriptions of four other putative enzymes involved in the glycolysis/gluconeogenesis pathway were altered in ospk[3].

Evolution

a single T-DNA was inserted in the transcriptional regulatory region of OsPK1 in ospk1. Quantitative RT-PCR result showed that OsPK1 expression was decreased by approximately 90% in ospk1 compared with that in WT [2].

Labs working on this gene

  • Institute of microbiology ,Chinese academy of sciences.
  • International Rice Research Institute, Manila, DAPO Box 7777, Philippines

References

<references> [1] [2] [3]

Structured Information

Gene Name

Os04g0677500

Description

Pyruvate kinase (EC 2.7.1.40)

Version

NM_001060800.1 GI:115461329 GeneID:4337406

Length

3615 bp

Definition

Oryza sativa Japonica Group Os04g0677500, complete gene.

Source

Oryza sativa Japonica Group 

 ORGANISM  Oryza sativa Japonica Group
           Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
           Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
           clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome

Chromosome 4

Location

Chromosome 4:35005323..35008937

Sequence Coding Region

35005655..35006467,35007045..35007506,35008380..35008640

Expression

GEO Profiles:Os04g0677500

Genome Context

<gbrowseImage1> name=NC_008397:35005323..35008937 source=RiceChromosome04 preset=GeneLocation </gbrowseImage1>

Gene Structure

<gbrowseImage2> name=NC_008397:35005323..35008937 source=RiceChromosome04 preset=GeneLocation </gbrowseImage2>

Coding Sequence

<cdnaseq>atggcgaacatcgacatggggaagatcctggcggggctggagaacgacgacgcgcgggtgcccaagaccaagctggtctgcacgctcggcccggcctcccgctccgtccccatgctcgagaagctgctccgcgccgggatgaacgtcgcgcgcttcaacttctcccacggcacccacgagtaccaccaggagaccctcgacaacctccgccaggccatgcacaacaccggcgtcctctgcgccgtcatgctcgataccaagggtcctgagattcgtactggatttttgaaggatggcaagccaatcaagctaacaaagggtcaagaactcactgttaccaccgattatgagatcaagggtgatgagaacatgattaccatgagttacaagaaactgccagttgatgtgaagcctggaaatgtcattctctgcgccgatggtacaatctctttgactgttttgtcctgtgatccaaaggctggaactgtgaggtgtaggtgtgagaacacagcaatgcttggcgagagaaagaattgcaatctgccaggaattgttgtggaccttcctacactgactgagaaggataaagaagacattttgggatggggtgtgccaaatgacatagacatgattgctctgtcgtttgtccgtaaaggatcagatttggttaccgtcagacaacttcttggacagcatgcaaagcgcatcaagctgatgtcaaaggttgaaaaccaagagggtgttgtaaacttcgatgagatcttgagggaaacggatgcatttatggttgctagaggtgatcttggaatggagattccagttgagaagatattccttgcacagaagatgatgatttacaagtgcaaccttgctggaaagcctgttgtgactgctactcagatgcttgagtcgatgatcaaatcaccacgtccaactcgtgctgaggcaactgacgttgcaaatgcagttcttgatggaactgactgcgtcatgcttagtggagagagtgctgctggagcataccctgaagtagctgtgaagatcatggcacgtatatgtgttgaggcagagtcttcccttgacaacgaagctgtcttcaaggagatgatcaggtctgcgccccttccgatgagcccattggagtctctcgcatcctctgctgtacgcacagccaacaaggccaaggcagccctgattgttgtcttgactcgtggtggtaccacggcaaagctggttgccaagtatcgtcccagggttccaatcctctctgtggttgtccccgtgttgacaaccgattcattcgactggacaatcagctcggagggcccagcaaggcacagcctaatctacagaggtcttgttcctctcctggctgagggttctgccaaagccaccgattcggagtcgacagaggtcatccttgatgctgctctcaagtcagctgtacagaagcagttgtgcaagcctggtgatgctgttgttgctctgcaccgtattggcgtcgcatccgtgatcaagatctgcatcgtgaagtaa</cdnaseq>

Protein Sequence

<aaseq>MANIDMGKILAGLENDDARVPKTKLVCTLGPASRSVPMLEKLLR AGMNVARFNFSHGTHEYHQETLDNLRQAMHNTGVLCAVMLDTKGPEIRTGFLKDGKPI KLTKGQELTVTTDYEIKGDENMITMSYKKLPVDVKPGNVILCADGTISLTVLSCDPKA GTVRCRCENTAMLGERKNCNLPGIVVDLPTLTEKDKEDILGWGVPNDIDMIALSFVRK GSDLVTVRQLLGQHAKRIKLMSKVENQEGVVNFDEILRETDAFMVARGDLGMEIPVEK IFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSG ESAAGAYPEVAVKIMARICVEAESSLDNEAVFKEMIRSAPLPMSPLESLASSAVRTAN KAKAALIVVLTRGGTTAKLVAKYRPRVPILSVVVPVLTTDSFDWTISSEGPARHSLIY RGLVPLLAEGSAKATDSESTEVILDAALKSAVQKQLCKPGDAVVALHRIGVASVIKIC IVK</aaseq>

Gene Sequence

<dnaseqindica>2471..3283#1432..1893#298..558#acctcgaccctctgcccctcaactcggtggtgaccacgcgtccacatctctcatctttctttctccgctattctcctccagtcctcttctcttcttcctccctcccttcgcccactgaatcgccaaaaaaattcccctcctccactccatctctataaaaatcctcccccaaatctcgcctcgtttagccagcacaactcacacaactctctctctctctctctctctctctctctcgtcgtctgcaagcttccgcctcccggatcgcggcgaccaccggaggaggagggagcagccatggcgaacatcgacatggggaagatcctggcggggctggagaacgacgacgcgcgggtgcccaagaccaagctggtctgcacgctcggcccggcctcccgctccgtccccatgctcgagaagctgctccgcgccgggatgaacgtcgcgcgcttcaacttctcccacggcacccacgagtaccaccaggagaccctcgacaacctccgccaggccatgcacaacaccggcgtcctctgcgccgtcatgctcgataccaaggttaccccctcactgctttgcttgtcgatctctacatgcttgtatgctgctgggttgttcggatcggaccagtcggggtagcttgcgtttgatacagagcggatttcgatttggatctgctcgtcttttttatcgggagaagctgggtttgatttggaggcagcatggtaggattatgagttgagtttacagcggtttgactttggaggactcgacaaaatggtatccggggttagatttgcctgcgcttgcgcttttgccttgtcgcagatggctggatcttggattgggggctcaggcttgcgtctccatgtgggtagttagttttggagtcttatggattctgagcaagtgtaagtgtgtaactggttcgtagtgtgatccgtttgcggtgttggtgagattgatgagctgcgtgcatcttgtttgtcatatttagctttacagttcgtctctatgctttttcaagttaatttattcatttgtagagttggattggaaggatggttaacagatcttgggatcggtggctttggagtatggagtgttcaaatcctagtgttctgattttgttgcttttgtaggaggatatgattaacacgtaaaagaaggctttattagtgtagtcatttcttctattatgccttgatgtttctgtcatgcacgtaactattggtgcttatctttttacactctcacacttcatggtttgctttgtagttcaaataagttacttacagaattgtactgcacacagtttatcttttttatcacttcttgtactgcaattagttccattatttttacctgtttagtagcatatttaaggcgattctgtggtgactgttctgaacatttcattcattgcttcagggtcctgagattcgtactggatttttgaaggatggcaagccaatcaagctaacaaagggtcaagaactcactgttaccaccgattatgagatcaagggtgatgagaacatgattaccatgagttacaagaaactgccagttgatgtgaagcctggaaatgtcattctctgcgccgatggtacaatctctttgactgttttgtcctgtgatccaaaggctggaactgtgaggtgtaggtgtgagaacacagcaatgcttggcgagagaaagaattgcaatctgccaggaattgttgtggaccttcctacactgactgagaaggataaagaagacattttgggatggggtgtgccaaatgacatagacatgattgctctgtcgtttgtccgtaaaggatcagatttggttaccgtcagacaacttcttggacagcatgcaaagcgcatcaagctgatgtcaaaggtttgtaatcattatcactattttttgggtcctgttaggttttcagttccacagtttgcttaagtatttaattaccaggacggttgagtgacaatacttctcagttagatgctttaattaatgttttgttgtccctttctggtatatactggagccagaattgtttggtctctgcctttgtacattgaccatttgacccctttttgtgctatattaggtaataaaatgcaattttgttccttttctatgtgcttgaacaactagtttcagaataaccttttttcacgtttgcttgttcaaatagcagctacttattttctcatgcactttcactgatagggtttagcttgttgcctgatatgcattaaaatgaggaattcaggttattgcatttctttgctgctaactgaaattttggatctagatggttcaccgtgttataaatgacctgtcttactgccatgatgataaaatatattcgtgatgatgataatacatatgaggtgcctggtaatttaccatgctggttatgttgctgtgcgtctaatttctttcttaactttttccttgtttttaggttgaaaaccaagagggtgttgtaaacttcgatgagatcttgagggaaacggatgcatttatggttgctagaggtgatcttggaatggagattccagttgagaagatattccttgcacagaagatgatgatttacaagtgcaaccttgctggaaagcctgttgtgactgctactcagatgcttgagtcgatgatcaaatcaccacgtccaactcgtgctgaggcaactgacgttgcaaatgcagttcttgatggaactgactgcgtcatgcttagtggagagagtgctgctggagcataccctgaagtagctgtgaagatcatggcacgtatatgtgttgaggcagagtcttcccttgacaacgaagctgtcttcaaggagatgatcaggtctgcgccccttccgatgagcccattggagtctctcgcatcctctgctgtacgcacagccaacaaggccaaggcagccctgattgttgtcttgactcgtggtggtaccacggcaaagctggttgccaagtatcgtcccagggttccaatcctctctgtggttgtccccgtgttgacaaccgattcattcgactggacaatcagctcggagggcccagcaaggcacagcctaatctacagaggtcttgttcctctcctggctgagggttctgccaaagccaccgattcggagtcgacagaggtcatccttgatgctgctctcaagtcagctgtacagaagcagttgtgcaagcctggtgatgctgttgttgctctgcaccgtattggcgtcgcatccgtgatcaagatctgcatcgtgaagtaatcgctacacggtgtcttcttagcgccattttgctgggttaagtggcaaaacaaaaactgctatgcttgtttttactcccatagtccatctttgggagacaatttaattaccgtatcagcccctgcagttcgcagggtgcctttcgttacatagatttgctggtaaaatgaggagagatagaactccattatgtagagctctatttatcgtcgatgccatgtagtatggcaggtggtttctattccatgcctgcacaattttctggtagctactgtgttgtttgattactgttttttcaggtgatgtcgatggagttgataataatattggca</dnaseqindica>

External Link(s)

NCBI Gene:Os04g0677500, RefSeq:Os04g0677500

  1. ↑ 1.0 1.1 1.2 .吴金山1， 张艳2， 朱传凤2， 罗丽娟1.4 个水稻线粒体丙酮酸激酶基因（mtOsPKs）表达分析.热带作物学报.Vol．31 No．5.
  2. ↑ 2.0 2.1 2.2 2.3 Yan Zhang • Wenkai Xiao • Lijuan Luo •Jinhuan Pang • Wei Rong • Chaozu He, Downregulation of OsPK1, a cytosolic pyruvate kinase, by T-DNA insertion causes dwarfism and panicle enclosure in rice.Planta (2012) 235:25–38.
  3. ↑ 3.0 3.1 3.2 Yan Zhang • Wenkai Xiao • Lijuan Luo • Jinhuan Pang • Wei Rong • Chaozu He.Downregulation of OsPK1, a cytosolic pyruvate kinase, by T-DNA insertion causes dwarfism and panicle enclosure in rice.Planta (2012) 235:25–38.
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